CHRNB4 (NM_000750) Human Untagged Clone

CAT#: SC310321

CHRNB4 (untagged)-Human cholinergic receptor, nicotinic, beta 4 (CHRNB4)



  "NM_000750" in other vectors (6)

CNY 3,656.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_000750 edited
CGGCGGCTGCCACCCGGCCCCGCCGGCCATGAGGCGCGCGCCTTCCCTGGTCCTTTTCTT
CCTGGTCGCCCTTTGCGGGCGCGGGAACTGCCGCGTGGCCAATGCGGAGGAAAAGCTGAT
GGACGACCTTCTGAACAAAACCCGTTACAATAACCTGATCCGCCCAGCCACCAGCTCCTC
ACAGCTCATCTCCATCAAGCTGCAGCTCTCCCTGGCCCAGCTTATCAGCGTGAATGAGCG
AGAGCAGATCATGACCACCAATGTCTGGCTGAAACAGGAATGGACTGATTACCGCCTGAC
CTGGAACAGCTCCCGCTACGAGGGTGTGAACATCCTGAGGATCCCTGCAAAGCGCATCTG
GTTGCCTGACATCGTGCTTTACAACAACGCCGACGGGACCTATGAGGTGTCTGTCTACAC
CAACTTGATAGTCCGGTCCAACGGCAGCGTCCTGTGGCTGCCCCCTGCCATCTACAAGAG
TGCCTGCAAGATTGAGGTGAAGTACTTTCCCTTCGACCAGCAGAACTGCACCCTCAAGTT
CCGCTCCTGGACCTATGACCACACGGAGATAGACATGGTCCTCATGACGCCCACAGCCAG
CATGGATGACTTTACTCCCAGTGGTGAGTGGGACATAGTGGCCCTCCCAGGGAGAAGGAC
AGTGAACCCACAAGACCCCAGCTACGTGGACGTGACTTACGACTTCATCATCAAGCGCAA
GCCTCTGTTCTACACCATCAACCTCATCATCCCCTGCGTGCTCACCACCTTGCTGGCCAT
CCTCGTCTTCTACCTGCCATCCGACTGCGGCGAGAAGATGACACTGTGCATCTCAGTGCT
GCTGGCACTGACATTCTTCCTGCTGCTCATCTCCAAGATCGTGCCACCCACCTCCCTCGA
TGTGCCTCTCATCGGCAAGTACCTCATGTTCACCATGGTGCTGGTCACCTTCTCCATCGT
CACCAGCGTCTGTGTGCTCAATGTGCACCACCGCTCGCCCAGCACCCACACCATGGCACC
CTGGGTCAAGCGCTGCTTCCTGCACAAGCTGCCTACCTTCCTCTTCATGAAGCGCCCTGG
CCCCGACAGCAGCCCGGCCAGAGCCTTCCCGCCCAGCAAGTCATGCGTGACCAAGCCCGA
GGCCACCGCCACCTCCACCAGCCCCTCCAACTTCTATGGGAACTCCATGTACTTTGTGAA
CCCCGCCTCTGCAGCTTCCAAGTCTCCAGCCGGCTCTACCCCGGTGGCTATCCCCAGGGA
TTTCTGGCTGCGGTCCTCTGGGAGGTTCCGACAGGATGTGCAGGAGGCATTAGAAGGTGT
CAGCTTCATCGCCCAGCACATGAAGAATGACGATGAAGACCAGAGTGTCGTTGAGGACTG
GAAGTACGTGGCTATGGTGGTGGACCGGCTGTTCCTGTGGGTGTTCATGTTTGTGTGCGT
CCTGGGCACTGTGGGGCTCTTCCTACCGCCCCTCTTCCAGACCCATGCAGCTTCTGAGGG
GCCCTACGCTGCCCAGCGTGACTGAGGGCCCCCTGGGTTGTGGGGTGAGAGGATGTGAGT
GGCCGGGTGGGCACTTTGCTGCTTCTTTCTGGGTTGTGGCCAAGGGCGAATTCAGATCTG
GTACCGATATCAAGCTTGT
>OriGene 5' read for NM_000750 unedited
GTGACGTCAAATTTTGTATACGACTCACTATAGGGCGGCCGCGATTCGCCCTTCTGTGAC
CCCACAGCGGAGCTCGCGGCGGCTGCCACCCGGCCCCGCCGGCCATGAGGCGCGCGCCTT
CCCTGGTCCTTTTCTTCCTGGTCGCCCTTTGCGGGCGCGGGAACTGCCGCGTGGCCAATG
CGGAGGAAAAGCTGATGGACGACCTTCTGAACAAAACCCGTTACAATAACCTGATCCGCC
CAGCCACCAGCTCCTCACAGCTCATCTCCATCAAGCTGCAGCTCTCCCTGGCCCAGCTTA
TCAGCGTGAATGAGCGAGAGCAGATCATGACCACCAATGTCTGGCTGAAACAGGAATGGA
CTGATTACCGCCTGACCTGGAACAGCTCCCGCTACGAGGGTGTGAACATCCTGAGGATCC
CTGCAAAGCGCATCTGGTTGCCTGACATCGTGCTTTACAACAACGCCGACGGGACCTATG
AGGTGTCTGTCTACACCAACTTGATAGTCCGGTCCAACGGCAGCGTCCTGTGGCTGCCCC
CTGCCATCTACAAGAGTGCCTGCAAGATTGAGGTGAAGTACTTTCCCTTCGACCAGCAGA
ACTGCACCCTCAAGTTCCGCTCCTGGACCTATGACCACACGGAGATAGACATGGTCCTCA
TGACGCCCACAGCCAGCATGGATGACTTTACTCCCAGTGGTGAGTGGGACATAGTGGCCC
TCCCAGGGAGAAGGACAGTGAACCCACAAGACCCCAGCTACGTGGACGTGACTTACGACT
TCATCATCAAGCGCAAGCCTCTGTTCTACACCATCAACCCTATAATCCCCTGCGTGCTCC
CCAACTTGCTGGCCATCCTTGTCTTCTACCTTGCATCCCAATGGCGGGGGAA
>OriGene 3' read for NM_000750 unedited
GGGGACATTGGNNGATGGCACTTNCCAGGNCCAGNANGAGCACTGGGGNAGGGTCACAGG
GATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAC
AAGCTTGATATCGGTACCAGATCTGAATTCGCCCTTGGCCACAACCCAGAAAGAAGCAGC
AAAGTGCCCACCCGGCCACTCACATCCTCTCACCCCACAACCCAGGGGGCCCTCAGTCAC
GCTGGGCAGCGTAGGGCCCCTCAGAAGCTGCATGGGTCTGGAAGAGGGGCGGTAGGAAGA
GCCCCACAGTGCCCAGGACGCACACAAACATGAACACCCACAGGAACAGCCGGTCCACCA
CCATAGCCACGTACTTCCAGTCCTCAACGACACTCTGGTCTTCATCGTCATTCTTCATGT
GCTGGGCGATGAAGCTGACACCTTCTAATGCCTCCTGCACATCCTGTCGGAACCTCCCAG
AGGACCGCAGCCAGAAATCCCTGGGGATAGCCACCGGGGTAGAGCCGGCTGGAGACTTGG
AAGCTGCAGAGGCGGGGTTCACAAAGTACATGGAGTTCCCATAGAAGTTGGAGGGGCTGG
TGGAGGTGGCGGTGGCCTCGGGCTTGGTCACGCATGACTTGCTGGGCGGGAAGGCTCTGG
CCGGGCTGCTGTCGGGGCCAGGGCGCTTCATGAAGAGGAAGGTAGGCAGCTTGTGCAGGA
AGCAGCGCTTGACCCAGGGTGCCATGGTGTGGGTGCTGGGCGAGCGGTGGTGCACATTGA
GCACACAGACGCTGGTGACGATGGAGAAAGTGACCAGCACCATGGTGAACATGAGGTACT
TGCCGATGAGAGGCACATCGAGGGAGGTGGGGTGGCACGATCTTGGAGATGAGG
Restriction Sites Please inquire     
ACCN NM_000750
Insert Size 1497 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000750.2, NP_000741.1
RefSeq Size 2447 bp
RefSeq ORF 1497 bp
Locus ID 1143
UniProt ID P30926
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Gene Summary This gene is found within a conserved gene cluster and encodes one of the beta subunits of the nicotinic acetylcholine receptor (nAChRs) superfamily which form ligand-gated ion channels with a central pore that forms a cation channel. Neuronal nAChRs are pentameric structures that can be either homomeric or heteromeric, with heteromeric structures containing both alpha and beta subunits. Each subunit contains an extracellular amino terminus and four transmembrane domains. Nicotine is one of the agonists that binds to the receptor. Variants in this gene have been associated with nicotine dependence and lung cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]
Transcript Variant: This variant (1) encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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