PFKFB2 (NM_006212) Human Untagged Clone

CAT#: SC310311

PFKFB2 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2), transcript variant 1



  "NM_006212" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-PFKFB2 Antibody
    • 100 ug

CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms PFK-2/FBPase-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310311 representing NM_006212.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCTGGGGCATCTTCCTCAGAACAGAACAACAACAGCTATGAAACCAAAACCCCAAATCTTCGAATG
TCAGAGAAGAAATGTTCATGGGCCTCCTACATGACCAACTCCCCGACTCTGATCGTTATGATTGGTTTG
CCAGCCCGGGGTAAAACCTACGTGTCCAAGAAACTAACACGCTACCTCAACTGGATTGGAGTCCCCACC
AAAGTGTTTAATCTTGGGGTGTATCGGCGTGAAGCAGTCAAGTCCTATAAGTCCTACGACTTCTTTCGG
CATGACAATGAGGAGGCCATGAAGATCCGCAAACAGTGTGCTCTGGTGGCGCTGGAAGATGTTAAGGCG
TATCTCACTGAGGAGAATGGTCAGATTGCGGTGTTTGATGCCACCAATACAACCCGGGAGAGGAGGGAC
ATGATTTTGAACTTTGCTGAACAGAATTCCTTCAAGGTATTCTTTGTGGAATCCGTCTGTGATGATCCT
GATGTCATTGCTGCCAATATTCTGGAGGTTAAGGTATCAAGCCCTGACTATCCTGAAAGGAACAGAGAG
AACGTGATGGAGGACTTCCTGAAGAGAATTGAATGCTACAAAGTTACCTACCGACCTCTTGACCCAGAC
AACTATGACAAGGATCTTTCTTTCATCAAGGTGATAAACGTGGGCCAGCGATTTTTAGTCAACAGAGTC
CAGGACTACATCCAGAGCAAGATAGTCTACTACCTCATGAATATCCACGTCCAGCCTCGCACCATTTAC
CTTTGCCGGCATGGAGAAAGCGAGTTCAATCTCTTGGGGAAGATTGGGGGTGACTCTGGCCTCTCGGTG
CGGGGAAAGCAGTTTGCCCAAGCTCTAAGGAAATTTCTGGAGGAACAGGAAATAACAGACCTCAAAGTG
TGGACAAGCCAGTTGAAGAGGACCATACAGACTGCTGAATCTCTCGGGGTGCCCTATGAGCAGTGGAAG
ATTCTGAATGAGATTGATGCTGGTGTGTGTGAAGAGATGACCTATGCAGAGATTGAGAAACGGTACCCA
GAAGAGTTTGCACTTCGAGATCAAGAGAAGTATCTGTATCGATATCCTGGTGGGGAGTCATACCAGGAC
CTGGTGCAGCGGCTGGAGCCTGTCATCATGGAGCTGGAACGTCAGGGCAATGTCCTCGTCATCTCCCAC
CAGGCTGTCATGCGCTGCCTCCTGGCCTACTTCTTGGATAAGGGCGCAGATGAGCTACCATACTTGAGA
TGCCCTCTCCATACCATCTTCAAACTTACTCCTGTGGCCTATGGGTGCAAAGTGGAAACAATTAAACTT
AACGTGGAGGCTGTGAACACGCACCGTGACAAGCCAACTAACAACTTCCCCAAGAACCAAACCCCTGTA
AGGATGAGAAGGAACAGCTTTACGCCTCTGTCCAGTTCGAATACAATAAGGCGTCCAAGAAATTACAGT
GTTGGGAGCCGGCCCCTCAAGCCCCTCAGCCCTCTCCGTGCCCAGGACATGCAAGAAGGGGCCGACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_006212
Insert Size 1518 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006212.2
RefSeq Size 7073 bp
RefSeq ORF 1518 bp
Locus ID 5208
UniProt ID O60825
Domains PGAM, 6PF2K
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism
MW 58.5 kDa
Gene Summary The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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