RASGRP3 (NM_170672) Human Untagged Clone
CAT#: SC310169
RASGRP3 (untagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GRP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC310169 representing NM_170672.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATTGAGATGTTT GATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTGATGCACCGATGGTATTTA TCTTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGAAATGCCACTGGAGAAAGCTGCAATGAA TTTCGATTAAAGATCTGCTACTTCATGAGGTACTGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGAT CTTGGTTTGATTCGTATGACTGAGGAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTC AGCCTCATCGACATATCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTA TCCAAGAAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACT TTTCTGGAGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTCATCCATGGCTGC CTGGAGAATAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGAATCTCTAAGTGGGTCCAGTTG ATGGTTCTTAGCAAACCAACCCCCCAGCAAAGGGCAGAAGTCATCACAAAGTTTATCAATGTTGCAAAG AAGCTCCTTCAGCTCAAAAATTTTAACACCCTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATT TCACGCCTCAAAGAGACCCATTCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAG TTGGTCTCCTCCAACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATC CCCATCCTTGGAGTACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACAGAGGAG AACAAAGTGAACATTGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTAGTCTCCCTGCAGAAT GCCTCTCACCACTTAGAACCCAACATGGATTTGATCAACCTGCTCACGCTTTCCCTGGACCTCTATCAC ACTGAAGATGATATTTACAAACTGTCACTGGTGCTGGAGCCTAGAAATTCTAAATCGCAGCCTACCTCC CCTACGACGCCCAACAAGCCTGTGGTACCCCTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCC ACGGTCATCAACAAGCACATAAGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGAT GGGTACATTTCCCAAGAGGACTTTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTT CTGGACAAAGATCAGGATGGCCTAATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCTAAATCC CAACTACACTGTAAAATGGGACCAGGATTTATCCATAATTTTCAGGAGATGACCTATCTCAAGCCAACC TTCTGCGAACACTGTGCGGGATTTCTCTGGGGCATAATCAAGCAAGGATACAAATGCAAAGACTGTGGA GCCAATTGTCACAAACAGTGCAAAGACCTCCTGGTTCTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCC TTGAGCAGTGGTCATGGGTCACTGCCTGGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAG TTCCCTGGAGTCACTGCTGGACACAGGGATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCT CGCAAGATCTCTGTGAGGCTACAGAGGGCCACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCA GAGGCTGGCTGGGGGGACTCGGGGTCCCACACCTTCCCTAAAATGAAATCCAAGTTCCATGACAAAGCA GCAAAGGACAAAGGCTTTGCCAAATGGGAAAATGAGAAGCCCAGGGTGCATGCTGGTGTGGATGTTGTA GACCGGGGCACGGAGTTTGAACTTGACCAGGATGAAGGAGAAGAGACCAGACAGGATGGTGAGGATGGC TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_170672 |
Insert Size | 2073 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_170672.2 |
RefSeq Size | 4711 bp |
RefSeq ORF | 2073 bp |
Locus ID | 25780 |
UniProt ID | Q8IV61 |
Domains | RasGEFN, RasGEF, EFh, DAG_PE-bind |
Protein Pathways | B cell receptor signaling pathway, MAPK signaling pathway |
MW | 78.3 kDa |
Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 4-6 all encode the same isoform (1). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206487 | RASGRP3 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2 |
CNY 5,056.00 |
|
RC206487L1 | Lenti ORF clone of Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, Myc-DDK-tagged |
CNY 7,456.00 |
|
RC206487L2 | Lenti ORF clone of Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC206487L3 | Lenti ORF clone of Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206487L4 | Lenti ORF clone of Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG206487 | RASGRP3 (tGFP-tagged) - Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2 |
CNY 6,656.00 |
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