RASGRP3 (NM_170672) Human Untagged Clone

CAT#: SC310169

RASGRP3 (untagged)-Human RAS guanyl releasing protein 3 (calcium and DAG-regulated) (RASGRP3), transcript variant 2



  "NM_170672" in other vectors (6)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
RASGRP3 mouse monoclonal antibody, clone OTI2C11 (formerly 2C11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GRP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC310169 representing NM_170672.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGATCAAGTGGCCTTGGGAAAGCAGCAACATTAGATGAACTGCTGTGCACTTGCATTGAGATGTTT
GATGACAATGGAGAGCTGGATAATAGTTATTTGCCAAGAATAGTTCTACTGATGCACCGATGGTATTTA
TCTTCCACTGAATTGGCAGAAAAACTTCTCTGCATGTATCGAAATGCCACTGGAGAAAGCTGCAATGAA
TTTCGATTAAAGATCTGCTACTTCATGAGGTACTGGATTCTGAAGTTTCCTGCAGAGTTTAATTTGGAT
CTTGGTTTGATTCGTATGACTGAGGAATTTCGGGAAGTAGCTAGTCAACTAGGATATGAAAAACACGTC
AGCCTCATCGACATATCCAGCATTCCTTCCTATGACTGGATGAGAAGAGTCACACAGAGGAAAAAAGTA
TCCAAGAAGGGAAAAGCCTGTCTGCTGTTTGACCATCTGGAGCCCATTGAATTGGCTGAGCACCTCACT
TTTCTGGAGCATAAATCTTTTAGAAGGATCTCATTCACTGATTACCAAAGCTATGTCATCCATGGCTGC
CTGGAGAATAATCCAACCTTGGAAAGATCGATTGCTTTATTTAATGGAATCTCTAAGTGGGTCCAGTTG
ATGGTTCTTAGCAAACCAACCCCCCAGCAAAGGGCAGAAGTCATCACAAAGTTTATCAATGTTGCAAAG
AAGCTCCTTCAGCTCAAAAATTTTAACACCCTGATGGCAGTGGTGGGAGGCCTCAGTCATAGTTCCATT
TCACGCCTCAAAGAGACCCATTCTCATCTTTCTTCAGAAGTTACAAAGAACTGGAATGAAATGACAGAG
TTGGTCTCCTCCAACGGCAATTACTGCAATTACCGCAAGGCCTTTGCCGACTGCGATGGCTTCAAAATC
CCCATCCTTGGAGTACACTTGAAAGACTTGATAGCTGTCCATGTCATTTTCCCAGACTGGACAGAGGAG
AACAAAGTGAACATTGTGAAAATGCACCAGCTCTCCGTTACCCTGAGTGAACTAGTCTCCCTGCAGAAT
GCCTCTCACCACTTAGAACCCAACATGGATTTGATCAACCTGCTCACGCTTTCCCTGGACCTCTATCAC
ACTGAAGATGATATTTACAAACTGTCACTGGTGCTGGAGCCTAGAAATTCTAAATCGCAGCCTACCTCC
CCTACGACGCCCAACAAGCCTGTGGTACCCCTGGAGTGGGCATTAGGGGTGATGCCAAAGCCAGACCCC
ACGGTCATCAACAAGCACATAAGGAAATTAGTGGAGTCTGTATTTAGAAACTATGATCACGACCATGAT
GGGTACATTTCCCAAGAGGACTTTGAAAGTATAGCTGCCAATTTTCCCTTCTTGGATTCCTTCTGTGTT
CTGGACAAAGATCAGGATGGCCTAATTAGTAAAGATGAAATGATGGCTTACTTCCTGAGAGCTAAATCC
CAACTACACTGTAAAATGGGACCAGGATTTATCCATAATTTTCAGGAGATGACCTATCTCAAGCCAACC
TTCTGCGAACACTGTGCGGGATTTCTCTGGGGCATAATCAAGCAAGGATACAAATGCAAAGACTGTGGA
GCCAATTGTCACAAACAGTGCAAAGACCTCCTGGTTCTGGCCTGCAGGAGATTTGCCCGGGCGCCCTCC
TTGAGCAGTGGTCATGGGTCACTGCCTGGAAGCCCCTCGCTGCCCCCAGCGCAGGATGAGGTGTTTGAG
TTCCCTGGAGTCACTGCTGGACACAGGGATTTAGACAGCAGAGCCATCACACTGGTTACAGGCTCTTCT
CGCAAGATCTCTGTGAGGCTACAGAGGGCCACCACCAGCCAGGCCACCCAGACTGAACCTGTCTGGTCA
GAGGCTGGCTGGGGGGACTCGGGGTCCCACACCTTCCCTAAAATGAAATCCAAGTTCCATGACAAAGCA
GCAAAGGACAAAGGCTTTGCCAAATGGGAAAATGAGAAGCCCAGGGTGCATGCTGGTGTGGATGTTGTA
GACCGGGGCACGGAGTTTGAACTTGACCAGGATGAAGGAGAAGAGACCAGACAGGATGGTGAGGATGGC
TGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_170672
Insert Size 2073 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_170672.2
RefSeq Size 4711 bp
RefSeq ORF 2073 bp
Locus ID 25780
UniProt ID Q8IV61
Domains RasGEFN, RasGEF, EFh, DAG_PE-bind
Protein Pathways B cell receptor signaling pathway, MAPK signaling pathway
MW 78.3 kDa
Gene Summary The protein encoded by this gene is a guanine nucleotide exchange factor that activates the oncogenes HRAS and RAP1A. Defects in this gene have been associated with systemic lupus erythematosus and several cancers. [provided by RefSeq, Mar 2017]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 4-6 all encode the same isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...