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PAPOLG (NM_022894) Human Untagged Clone
CAT＃: SC310141
PAPOLG (untagged)-Human poly(A) polymerase gamma (PAPOLG)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC310141 representing NM_022894.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAGAGATGTCTGCAAACACCGTGCTGGACAGCCAGCGTCAACAAAAGCATTATGGAATTACCTCC CCAATTAGTTTGGCATCTCCTAAAGAAATTGATCATATTTACACACAGAAATTAATTGACGCCATGAAA CCATTTGGAGTGTTTGAAGATGAGGAAGAATTGAACCACAGGCTGGTGGTTCTTGGTAAATTGAACAAT TTAGTAAAAGAATGGATTTCTGATGTCAGCGAGAGTAAGAACCTCCCACCTTCTGTTGTGGCTACTGTT GGTGGTAAAATTTTCACATTTGGATCCTATAGGCTTGGAGTACACACCAAAGGAGCTGACATTGATGCA CTTTGTGTAGCTCCAAGACATGTGGAAAGATCTGATTTTTTTCAGTCTTTTTTTGAAAAATTGAAACAT CAAGATGGCATTAGAAACTTAAGAGCTGTAGAAGATGCCTTTGTACCTGTTATAAAATTTGAATTTGAT GGTATTGAAATTGATCTAGTCTTTGCAAGACTGGCAATACAAACCATATCAGATAATTTAGATCTAAGA GACGACTCTCGCCTGAGAAGCCTTGATATAAGGTGTATTCGCAGCTTAAATGGTTGTAGAGTTACTGAT GAAATTTTGCATTTAGTGCCAAATAAAGAAACTTTTAGACTCACCCTAAGAGCTGTCAAATTATGGGCA AAACGACGTGGTATTTATTCCAACATGCTAGGATTCCTTGGTGGTGTCTCCTGGGCAATGCTAGTTGCA AGAACTTGCCAATTGTATCCAAATGCAGCAGCATCTACTTTAGTTCATAAGTTCTTTTTAGTTTTTTCC AAGTGGGAATGGCCAAATCCTGTGCTGCTGAAGCAACCAGAAGAAAGCAATTTGAATTTGCCTGTCTGG GATCCTCGGGTAAATCCATCAGATAGGTATCATCTCATGCCCATAATCACCCCTGCCTACCCACAACAG AATTCTACGTATAATGTGTCCACATCAACTCGAACAGTAATGGTAGAAGAATTTAAACAAGGTCTTGCA GTCACAGATGAAATTCTTCAAGGAAAGTCAGATTGGTCCAAACTACTTGAGCCACCGAATTTCTTTCAA AAGTATAGACATTATATAGTATTGACTGCCAGCGCATCAACAGAAGAAAACCATCTAGAGTGGGTTGGA TTAGTAGAATCTAAAATCCGTGTACTTGTTGGAAACTTGGAACGGAATGAATTTATTACTCTTGCCCAT GTGAATCCCCAGTCATTCCCAGGGAATAAGGAACATCATAAAGACAACAATTACGTATCAATGTGGTTC CTTGGGATAATTTTTCGGAGAGTAGAAAATGCAGAAAGTGTCAACATAGACTTGACATATGATATACAG TCATTTACTGATACAGTGTACAGACAGGCAAACAATATAAATATGCTAAAGGAGGGAATGAAAATTGAA GCAACTCATGTAAAGAAAAAACAACTTCACCACTACCTTCCTGCAGAAATTCTTCAAAAGAAGAAAAAG CAAAGTCTCTCTGATGTCAATCGAAGCTCGGGCGGACTTCAATCCAAAAGATTGTCTCTGGATAGCAGT TGTCTGGATAGCTCCAGAGACACTGATAATGGAACACCTTTTAATTCTCCAGCGTCCAAGTCTGATAGC CCTTCTGTAGGAGAAACAGAAAGGAATAGTGCTGAGCCTGCTGCTGTAATTGTGGAGAAGCCACTGAGT GTACCACCAGCCCAAGGACTTTCCATTCCAGTGATTGGCGCAAAAGTTGACTCTACAGTAAAAACTGTA TCACCCCCCACTGTGTGTACCATTCCTACCGTAGTAGGACGAAATGTCATTCCTAGAATCACAACACCT CACAACCCTGCCCAGGGACAACCGCATCTGAATGGAATGTCAAATATAACTAAGACTGTTACACCTAAG AGATCCCATTCCCCATCCATAGATGGGACTCCTAAGAGGTTGAAAGACGTAGAAAAGTTTATTCGACTT GAATCAACATTTAAGGACCCCCGCACTGCTGAAGAAAGAAAAAGAAAATCAGTGGATGCCATTGGAGGA GAATCTATGCCTATTCCAACTATTGATACATCACGCAAAAAGAGACTACCCAGTAAAGAACTACCAGAT TCATCATCTCCAGTTCCAGCAAACAACATCCGTGTCATCAAAAATTCCATTCGACTGACCCTTAATCGG TAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022894 |
| Insert Size | 2211 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022894.3 |
| RefSeq Size | 7386 bp |
| RefSeq ORF | 2211 bp |
| Locus ID | 64895 |
| UniProt ID | Q9BWT3 |
| Domains | NTP_transf_2, PAP_RNA-bind, PAP_central |
| Protein Families | Transcription Factors |
| Protein Pathways | RNA degradation |
| MW | 82.8 kDa |
| Gene Summary | This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213918 | PAPOLG (Myc-DDK-tagged)-Human poly(A) polymerase gamma (PAPOLG) |
CNY 5,392.00 |
|
| RC213918L3 | Lenti ORF clone of Human poly(A) polymerase gamma (PAPOLG), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213918L4 | Lenti ORF clone of Human poly(A) polymerase gamma (PAPOLG), mGFP tagged |
CNY 5,890.00 |
|
| RG213918 | PAPOLG (tGFP-tagged) - Human poly(A) polymerase gamma (PAPOLG) |
CNY 6,992.00 |
