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PML Protein (PML) (NM_033249) Human Untagged Clone
CAT＃: SC310060
PML (untagged)-Human promyelocytic leukemia (PML), transcript variant 10
CNY 4,360.00
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MYL; PP8675; RNF71; TRIM19 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_033249 edited
AGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGA ATTCGGCACGAGGCGAGAATCGAAACTAAGCTGGGGTCCATGGAGCCTGCACCCGCCCGA TCTCCGAGGCCCCAGCAGGACCCCGCCCGGCCCCAGGAGCCCACCATGCCTCCCCCCGAG ACCCCCTCTGAAGGCCGCCAGCCCAGCCCCAGCCCCAGCCCTACAGAGCGAGCCCCCGCT TCGGAGGAGGAGTTCCAGTTTCTGCGCTGCCAGCAATGCCAGGCGGAAGCCAAGTGCCCG AAGCTGCTGCCTTGTCTGCACACGCTGTGCTCAGGATGCCTGGAGGCGTCGGGCATGCAG TGCCCCATCTGCCAGGCGCCCTGGCCCCTAGGTGCAGACACACCCGCCCTGGATAACGTC TTTTTCGAGAGTCTGCAGCGGCGCCTGTCGGTGTACCGGCAGATTGTGGATGCGCAGGCT GTGTGCACCCGCTGCAAAGAGTCGGCCGACTTCTGGTGCTTTGAGTGCGAGCAGCTCCTC TGCGCCAAGTGCTTCGAGGCACACCAGTGGTTCCTCAAGCACGAGGCCCGGCCCCTAGCA GAGCTGCGCAACCAGTCGGTGCGTGAGTTCCTGGACGGCACCCGCAAGACCAACAACATC TTCTGCTCCAACCCCAACCACCGCACCCCTACGCTGACCAGCATCTACTGCCGAGGATGT TCCAAGCCGCTGTGCTGCTCGTGCGCGCTCCTTGACAGCAGCCACAGTGAGCTCAAGTGC GACATCAGCGCAGAGATCCAGCAGCGACAGGAGGAGCTGGACGCCATGACGCAGGCGCTG CAGGAGCAGGATAGTGCCTTTGGCGCGGTTCACGCGCAGATGCACGCGGCCGTCGGCCAG CTGGGCCGCGCGCGTGCCGAGACCGAGGAGCTGATCCGCGAGCGCGTGCGCCAGGTGGTA GCTCACGTGCGGGCTCAGGAGCGCGAGCTGCTGGAGGCTGTGGACGCGCGGTACCAGCGC GACTACGAGGAGATGGCCAGTCGGCTGGGCCGCCTGGATGCTGTGCTGCAGCGCATCCGC ACGGGCAGCGCGCTGGTGCAGAGGATGAAGTGCTACGCCTCGGACCAGGAGGTGCTGGAC ATGCACGGTTTCCTGCGCCAGGCGCTCTGCCGCCTGCGCCAGGAGGAGCCCCAGAGCCTG CAAGCTGCCGTGCGCACCGATGGCTTCGACGAGTTCAAGGTGCGCCTGCAGGACCTCAGC TCTTGCATCACCCAGGGGAAAGATGCAGCTGTATCCAAGAAAGCCAGCCCAGAGGCTGCC AGCACTCCCAGGGACCCTATTGACGTTGACCTGGATGTCTCCAATACAACGACAGCCCAG AAGAGGAAGTGCAGCCAGACCCAGTGCCCCAGGAAGGTCATCAAGATGGAGTCTGAGGAG GGGAAGGAGGCAAGGTTGGCTCGGAGCTCCCCGGAGCAGCCCAGGCCCAGCACCTCCAAG GCAGTCTCACCACCCCACCTGGATGGACCGCCTAGCCCCAGGAGCCCCGTCATAGGAAGT GAGGTCTTCCTGCCCAACAGCAACCACGTGGCCAGTGGCGCCGGGGAGGCAGAGGAACGC GTTGTGGTGATCAGCAGCTCGGAAGACTCAGATGCCGAAAACTCGTCCTCCCGAGAGCTG GATGACAGCAGCAGTGAGTCCAGTGACCTCCAGCTGGAAGGCCCCAGCACCCTCAGGGTC CTGGACGAGAACCTTGCTGACCCCCAAGCAGAAGACAGACCTCTGGTTTTCTTTGACCTC AAGATTGACAATGAAACCCAGAAGATTAGCCAGCTGGCTGCGGTGAACCGGGAAAGCAAG TTCCGCGTGGTCATCCAGCCTGAAGCCCTCTTCAGCATCTACTCCAAGGCCGTGTCCCTG GAGGTGGGGCTGCAGCACTTCCTCAGCTTTCTGAGCTCCATGCGCCGCCCTATCTTGGCC TGCTACAAGCTGTGGGGGCCTGGCCTCCCAAACTTCTTCCGGGCCCTGGAGGACATTAAC AGGCTGTGGGAATTCCAGGAGGCCATCTCGGGCTTCCTGGCTGCCCTGCCTCTCATCCGG GAGCGTGTGCCCGGGGCCAGCAGCTTCAAACTCAAGAACCTGGCCCAGACCTACCTGGCG AGAAACATGAGCGAGCGCAGCGCCATGGCTGCCGTGCTGGCCATGCGTGACCTGTGCCGC CTCCTCGAGGTCTCCCCGGGCCCCCAGCTGGCCCAGCATGTCTACCCCTTCAGTAGCCTG CAGTGCTTTGCCTCCCTGCAGCCCCTGGTGCAGGCAGCTGTGCTGCCCCGGGCTGAGGCC CGCCTCCTGGCCCTACACAACGTGAGCTTCATGGAGCTGCTGAGTGCACACCGCCGTGAC CGGCAGGGGGGCCTGAAGAAGTACAGCCGCTATCTAAGCCTGCAGACCACCACGTTGCCC CCTGCCCAGCCTGCTTTCAACCTGCAGGCTCTGGGCACCTACTTTGAAGGCCTGTTGGAG GGTCCGGCGCTGGCACGGGCAGAAGGAGTCTCCACCCCACTTGCTGGCCGTGGCTTGGCA GAGAGGGCCTCCCAGCAGAGCTGAGAGGAGGGGGTGACCAGCTTGGAGTCTCTGGTGGGC AGAGAGGGATGGGGTCCCTGAGCCAGGCCCCACCCATCACAGCATTCCCAGGTCCTGGTA CCCA >OriGene 5' read for NM_033249 unedited
AGAAATTTGTAATACGACTCACTATAGGGGCGGCCGCGAATCGGCACGAGGCGAGAATCG AAACTAAGCTGGGGTCCATGGAGCCTGCACCCGCCCGATCTCCGAGGCCCCAGCAGGACC CCGCCCGGCCCCAGGAGCCCACCATGCCTCCCCCCGAGACCCCCTCTGAAGGCCGCCAGC CCAGCCCCAGCCCCAGCCCTACAGAGCGAGCCCCCGCTTCGGAGGAGGAGTTCCAGTTTC TGCGCTGCCAGCAATGCCAGGCGGAAGCCAAGTGCCCGAAGCTGCTGCCTTGTCTGCACA CGCTGTGCTCAGGATGCCTGGAGGCGTCGGGCATGCAGTGCCCCATCTGCCAGGCGCCCT GGCCCCTAGGTGCAGACACACCCGCCCTGGATAACGTCTTTTTCGAGAGTCTGCAGCGGC GCCTGTCGGTGTACCGGCAGATTGTGGATGCGCAGGCTGTGTGCACCCGCTGCAAAGAGT CGGCCGACTTCTGGTGCTTTGAGTGCGAGCAGCTCCTCTGCGCCAAGTGCTTCGAGGCAC ACCAGTGGTTCCTCAAGCACGAGGCCCGGCCCCTAGCAGAGCTGCGCAACCAGTCGGTGC GTGAGTTCCTGGACGGCACCCGCAAGACCAACAACATCTTCTGCTCCAACCCCAACCACC GCACCCCTACGCTGACCAGCATCTACTGCCGAGGATGTTCCAAGCCGCTGTGCTGCTCGT GCGCGCTCCTTGACAGCAGCCACAGTGAGCTCAAGTGCGACATCAGCGCAGAGATCCAGC AGCGACAGGAAGAGCTGGACGCCCATGACGCAGCGCTGCANGAGCANGATAGTGCCTTTG GCGCGGTNACGCGCAAATGCACGCGNCGTCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_033249 |
| Insert Size | 3900 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033249.2, NP_150252.1 |
| RefSeq Size | 2110 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 5371 |
| UniProt ID | P29590 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Acute myeloid leukemia, Pathways in cancer, Ubiquitin mediated proteolysis |
| Gene Summary | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (10) has multiple differences in the coding region compared to variant 1, one of which results in translational frame-shift. The resulting isoform (10, also known as PML-4A, PML-IVA and TRIM19lambda) has a distinct C-terminus and is shorter than isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224728 | PML (Myc-DDK-tagged)-Human promyelocytic leukemia (PML), transcript variant 10 |
CNY 5,800.00 |
|
| RC224728L3 | Lenti-ORF clone of PML (Myc-DDK-tagged)-Human promyelocytic leukemia (PML), transcript variant 10 |
CNY 7,700.00 |
|
| RC224728L4 | Lenti-ORF clone of PML (mGFP-tagged)-Human promyelocytic leukemia (PML), transcript variant 10 |
CNY 7,700.00 |
|
| RG224728 | PML (tGFP-tagged) - Human promyelocytic leukemia (PML), transcript variant 10 |
CNY 6,392.00 |
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