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AF4 (AFF1) (NM_005935) Human Untagged Clone
CAT＃: SC309949
AFF1 (untagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2
CNY 20,264.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AF4; MLLT2; PBM1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_005935 edited
CTGAATTATGGCAGCCCAGTCAAGTTTGTACAATGACGACAGAAACCTGCTTCGAATTAG AGAGAAGGAAAGACGCAACCAGGAAGCCCACCAAGAGAAAGAGGCATTTCCTGAAAAGAT TCCCCTTTTTGGAGAGCCCTACAAGACAGCAAAAGGTGATGAGCTGTCTAGTCGAATACA GAACATGTTGGGAAACTACGAAGAAGTGAAGGAGTTCCTTAGTACTAAGTCTCACACTCA TCGCCTGGATGCTTCTGAAAATAGGTTGGGAAAGCCGAAATATCCTTTAATTCCTGACAA AGGGAGCAGCATTCCATCCAGCTCCTTCCACACTAGTGTCCACCACCAGTCCATTCACAC TCCTGCGTCTGGACCACTTTCTGTTGGCAACATTAGCCACAATCCAAAGATGGCGCAGCC AAGAACTGAACCAATGCCAAGTCTCCATGCCAAAAGCTGCGGCCCACCGGACAGCCAGCA CCTGACCCAGGATCGCCTTGGTCAGGAGGGGTTCGGCTCTAGTCATCACAAGAAAGGTGA CCGAAGAGCTGACGGAGACCACTGTGCTTCGGTGACAGATTCGGCTCCAGAGAGGGAGCT TTCTCCCTTAATCTCTTTGCCTTCCCCAGTTCCCCCTTTGTCACCTATACATTCCAACCA GCAAACTCTTCCCCGGACGCAAGGAAGCAGCAAGGTTCATGGCAGCAGCAATAACAGTAA AGGCTATTGCCCAGCCAAATCTCCCAAGGACCTAGCAGTGAAAGTCCATGATAAAGAGAC CCCTCAAGACAGTTTGGTGGCCCCTGCCCAGCCGCCTTCTCAGACATTTCCACCTCCCTC CCTCCCCTCAAAAAGTGTTGCAATGCAGCAGAAGCCCACGGCTTATGTCCGGCCCATGGA TGGTCAAGATCAGGCCCCTAGTGAATCCCCTGAACTGAAACCACTGCCGGAGGACTATCG ACAGCAGACCTTTGAAAAAACAGACTTGAAAGTGCCTGCCAAAGCCAAGCTCACCAAACT GAAGATGCCTTCTCAGTCAGTTGAGCAGACCTACTCCAATGAAGTCCATTGTGTTGAAGA GATTCTGAAGGAAATGACCCATTCATGGCCGCCTCCTTTGACAGCAATACATACGCCTAG TACAGCTGAGCCATCCAAGTTTCCTTTCCCTACAAAGGACTCTCAGCATGTCAGTTCTGT AACCCAAAACCAAAAACAATATGATACATCTTCAAAAACTCACTCAAATTCTCAGCAAGG AACGTCATCCATGCTCGAAGACGACCTTCAGCTCAGTGACAGTGAGGACAGTGACAGTGA ACAAACCCCAGAGAAGCCTCCCTCCTCATCTGCACCTCCAAGTGCTCCACAGTCCCTTCC AGAACCAGTGGCATCAGCACATTCCAGCAGTGCAGAGTCAGAAAGCACCAGTGACTCAGA CAGTTCCTCAGACTCAGAGAGCGAGAGCAGTTCAAGTGACAGCGAAGAAAATGAGCCCCT AGAAACCCCAGCTCCGGAGCCTGAGCCTCCAACAACAAACAAATGGCAGCTGGACAACTG GCTGACCAAAGTCAGCCAGCCAGCTGCGCCACCAGAGGGCCCCAGGAGCACAGAGCCCCC ACGGCGGCACCCAGAGAGTAAGGGCAGCAGCGACAGTGCCACGAGTCAGGAGCATTCTGA ATCCAAAGATCCTCCCCCTAAAAGCTCCAGCAAAGCCCCCCGGGCCCCACCCGAAGCCCC CCACCCCGGAAAGAGGAGCTGTCAGAAGTCTCCGGCACAGCAGGAGCCCCCACAAAGGCA AACCGTTGGAACCAAACAACCCAAAAAACCTGTCAAGGCCTCTGCCCGGGCAGGTTCACG GACCAGCCTGCAGGGGGAAAGGGAGCCAGGGCTTCTTCCCTATGGCTCCCGAGACCAGAC TTCCAAAGACAAGCCCAAGGTGAAGACGAAAGGACGGCCCCGGGCCGCAGCAAGCAACGA ACCCAAGCCAGCAGTGCCCCCCTCCAGTGAGAAGAAGAAGCACAAGAGCTCCCTCCCTGC CCCCTCTAAGGCTCTCTCAGGCCCAGAACCCGCGAAGGACAATGTGGAGGACAGGACCCC TGAGCACTTTGCTCTTGTTCCCCTGACTGAGAGCCAGGGCCCACCCCACAGTGGCAGCGG CAGCAGGACTAGTGGCTGCCGCCAAGCCGTGGTGGTCCAGGAGGACAGCCGCAAAGACAG ACTCCCATTGCCTTTGAGAGACACCAAGCTGCTCTCACCGCTCAGGGACACTCCTCCCCC ACAAAGCTTGATGGTGAAGATCACCCTAGACCTGCTCTCTCGGATACCCCAGCCTCCCGG GAAGGGGAGCCGCCAGAGGAAAGCAGAAGATAAACAGCCGCCCGCAGGGAAGAAGCACAG CTCTGAGAAGAGGAGCTCAGACAGCTCAAGCAAGTTGGCCAAAAAGAGAAAGGGTGAAGC AGAAAGAGACTGTGATAACAAGAAAATCAGACTGGAGAAGGAAATCAAATCACAGTCATC TTCATCTTCATCCTCCCACAAAGAATCTTCTAAAACAAAGCCCTCCAGGCCCTCCTCACA GTCCTCAAAGAAGGAAATGCTCCCCCCGCCACCCGTGTCCTCGTCCTCCCAGAAGCCAGC CAAGCCTGCACTTAAGAGGTCAAGGCGGGAAGCAGACACCTGTGGCCAGGACCCTCCCAA AAGTGCCAGCAGTACCAAGAGCAACCACAAAGACTCTTCCATTCCCAAGCAGAGAAGAGT AGAGGGGAAGGGCTCCAGAAGCTCCTCGGAGCACAAGGGTTCTTCCGGAGATACTGCAAA TCCTTTTCCAGTGCCTTCTTTGCCAAATGGTAACTCTAAACCAGGGAAGCCTCAAGTGAA GTTTGACAAACAACAAGCAGACCTTCACATGAGGGAGGCAAAAAAGATGAAGCAGAAAGC AGAGTTAATGACGGACAGGGTTGGAAAGGCTTTTAAGTACCTGGAAGCCGTCTTGTCCTT CATTGAGTGCGGAATTGCCACAGAGTCTGAAAGCCAGTCATCCAAGTCAGCTTACTCTGT CTACTCAGAAACTGTAGATCTCATTAAATTCATAATGTCATTAAAATCCTTCTCAGATGC CACAGCGCCAACACAAGAGAAAATATTTGCTGTTTTATGCATGCGTTGCCAGTCCATTTT GAACATGGCGATGTTTCGTTGTAAAAAAGACATAGCAATAAAGTATTCTCGTACTCTTAA TAAACACTTCGAGAGTTCTTCCAAAGTCGCCCAGGCACCTTCTCCATGCATTGCAAGAAG CACAGGCACACCATCCCCTCTTTCCCCAATGCCTTCTCCTGCCAGCTCCGTAGGGTCCCA GTCAAGTGCTGGCAGTGTGGGGAGCAGTGGGGTGGCTGCCACTATCAGCACCCCAGTCAC CATCCAGAATATGACATCTTCCTATGTCACCATCACATCCCATGTTCTTACCGCCTTTGA CCTTTGGGAACAGGCCGAGGCCCTCACGAGGAAGAATAAAGAATTCTTTGCTCGGCTCAG CACAAATGTGTGCACCTTGGCCCTCAACAGCAGTTTGGTGGACCTGGTGCACTATACACG ACAGGGTTTTCAGCAGCTACAAGAATTAACCAAAACACCTTAATGGAGCCCCAGGTTGAT TCAATGCCTTGGGAACTATTTTTGCACATTGGAAGCCTCAAAAACAGTCCAGACGTTTGT TTCATCAGGACACCAAACTCTAAAAAAGAAGCACCACGAGATGGCCAGGACATTTGTCCA CTTAAACTCTCAACAACAGTGTGATCATTGGTTGGACACTGTGGTTATGCAGAAGCAGAG ATGAGGAGGCTGGCCCCAGAGATGATCTTGCCCTTCCTAACTAAAGGACAGAAGTGCAAT TTAGCTTAAATGGGTGTATGAATGGTCTAGAAACATTTCTATTTTTTTTTTAAACCAGCA GGATACAAGTTGCAAATGAAATGAGGAGAAACAGTTTCAACTCTGAAAGTGAATTTCACG TCATCTCAGTAGCCACGCTAGTCCATTCCCAGAAGGAAATTTTTTTTTTTAACAATGACT TTTGGTAAAGGGTTTTGTGGATGATTTTTTTTCTTTTGAGTTTTGGGAGAAATATTTGTT TAATAACTTCTAATGGCCATCTGTAAACCATAAGTAATGAAGGACTCCACTGTGCCCCAC TTTCTGCCAATGAACAGTGGCTTGATAATAAAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_005935 unedited
GGCACGTCGGATATTTGTATACGACTTATATAGGCGGCCGCGAATTCCCCTTTAACTGAA TATGGCAGCCCAGTCAAGTTTGTACAATGACGACAGAAACCTGCTTCGAATTAGAGAGAA GGAAAGACGCAACCAGGAAGCCCACCAAGAGAAAGAGGCATTTCCTGAAAAGATTCCCCT TTTTGGAGAGCCCTACAAGACAGCAAAAGGTGATGAGCTGTCTAGTCGAATACAGAACAT GTTGGGAAACTACGAAGAAGTGAAGGAGTTCCTTAGTACTAAGTCTCACACTCATCGCCT GGATGCTTCTGAAAATAGGTTGGGAAAGCCGAAATATCCTTTAATTCCTGACAAAGGGAG CAGCATTCCATCCAGCTCCTTCCACACTAGTGTCCACCACCAGTCCATTCACACTCCTGC GTCTGGACCACTTTCTGTTGGCAACATTAGCCACAATCCAAAGATGGCGCAGCCAAGAAC TGAACCAATGCCAAGTCTCCATGCCAAAAGCTGCGGCCCACCGGACAGCCAGCACCTGAC CCAGGATCGCCTTGGTCAGGAGGGGTTCGGCTCTAGTCATCACAAGAAAGGTGACCGAAG AGCTGACGGAGACCACTGTGCTTCGGTGACAGATTCGGCTCCAGAGAGGGAGCTTTCTCC CTTAATCTCTTTGCCTTCCCAGTTCCCCCTTTGTCACCTATACATTCCAACCAGCAAACT CTTCCCCGGACGCAAGGAAGCAGCAAAGGTCATGGCAGCAGCAATAACAGTAAAAGCTAT TGCCCAGCCCAACCTCCCAAGGACTAGCAATGAAAGTCCATGATAAAGAGACCCCTCAAG ACAGTTTGGTANCCCCCTGCCCAACCGCCTTCTCAAAATTTTCCACTTCCTCCCTCCCCT CAAA >OriGene 3' read for NM_005935 unedited
GGGGCGCCCTTTGGTGGATGGCAACTCCCCAGTTCCAGNANAGCACTGGGGCAGGGTCAC AGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTC GAGTTTTTTTTTTTTTTTTTTTTTATTATCAAGCCACTGTTCATTGGCAGAAAGTGGGGC ACAGTGGAGTCCTTCATTACTTATGGTTTACAGATGGCCATTAGAAGTTATTAAACAAAT ATTTCTCCCAAAACTCAAAAGAAAAAAAATCATCCACAAAACCCTTTACCAAAAGTCATT GTTAAAAAAAAAAATTTCCTTCTGGGAATGGACTAGCGTGGCTACTGAGATGACGTGAAA TTCACTTTCAGAGTTGAAACTGTTTCTCCTCATTTCATTTGCAACTTGTATCCTGCTGGT TTAAAAAAAAAATAGAAATGTTTCTAGACCATTCATACACCCATTTAAGCTAAATTGCAC TTCTGTCCTTTAGTTAGGAAGGGCAAGATCATCTCTGGGGCCAGCCTCCTCATCTCTGCT TCTGCATAACCACAGTGTCCAACCAATGATCACACTGTTGTTGAGAGTTTAAGTGGACAA ATGTCCTGGCCATCTCGTGGTGCTTCTTTTTTAGAGTTTGGTGTCCTGATGAAACAAACG TCTGGACTGTTTTTGAGGCTTCCCATGTGCAAAAATAGTTCCCAAGGCTTTGAATCAACC TGGGGCTCCTTTAAGTGTTTTGGTTATTCTTGTAGCTGCTGAAACCCTGTCGTGTTAGTG CCCCGGTCCACAAACTGCTGTTGAGGCCCAAGTGCAACATTTTTGCTGACCCCACCAAAA ATTCTTTATTTTCCTCGGGAGGCCTCGGCTTGTTCCAAAGTTAAAGGCGGTAAAAACTGG GTG |
| Restriction Sites | Please inquire |
| ACCN | NM_005935 |
| Insert Size | 4300 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and insertion of 1 aa. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005935.1, NP_005926.1 |
| RefSeq Size | 9390 bp |
| RefSeq ORF | 9378 bp |
| Locus ID | 4299 |
| UniProt ID | P51825 |
| Domains | AF-4 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (2) contains a distinct 5' UTR, uses an alternate translation start site, and uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217054 | AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 9,264.00 |
|
| RC217054L1 | Lenti-ORF clone of AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 11,664.00 |
|
| RC217054L2 | Lenti-ORF clone of AFF1 (mGFP-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 13,970.00 |
|
| RC217054L3 | Lenti-ORF clone of AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 11,664.00 |
|
| RC217054L4 | Lenti-ORF clone of AFF1 (mGFP-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 13,970.00 |
|
| RG217054 | AFF1 (tGFP-tagged) - Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 13,300.00 |
