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AF4 (AFF1) (NM_005935) Human Tagged ORF Clone
CAT＃: RC217054
- TrueORF®
AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2
ORF Plasmid: tGFP
"NM_005935" in other vectors (6)
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CNY 9,264.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AF4; MLLT2; PBM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217054 representing NM_005935
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCCAGTCAAGTTTGTACAATGACGACAGAAACCTGCTTCGAATTAGAGAGAAGGAAAGACGCA ACCAGGAAGCCCACCAAGAGAAAGAGGCATTTCCTGAAAAGATTCCCCTTTTTGGAGAGCCCTACAAGAC AGCAAAAGGTGATGAGCTGTCTAGTCGAATACAGAACATGTTGGGAAACTACGAAGAAGTGAAGGAGTTC CTTAGTACTAAGTCTCACACTCATCGCCTGGATGCTTCTGAAAATAGGTTGGGAAAGCCGAAATATCCTT TAATTCCTGACAAAGGGAGCAGCATTCCATCCAGCTCCTTCCACACTAGTGTCCACCACCAGTCCATTCA CACTCCTGCGTCTGGACCACTTTCTGTTGGCAACATTAGCCACAATCCAAAGATGGCGCAGCCAAGAACT GAACCAATGCCAAGTCTCCATGCCAAAAGCTGCGGCCCACCGGACAGCCAGCACCTGACCCAGGATCGCC TTGGTCAGGAGGGGTTCGGCTCTAGTCATCACAAGAAAGGTGACCGAAGAGCTGACGGAGACCACTGTGC TTCGGTGACAGATTCGGCTCCAGAGAGGGAGCTTTCTCCCTTAATCTCTTTGCCTTCCCCAGTTCCCCCT TTGTCACCTATACATTCCAACCAGCAAACTCTTCCCCGGACGCAAGGAAGCAGCAAGGTTCATGGCAGCA GCAATAACAGTAAAGGCTATTGCCCAGCCAAATCTCCCAAGGACCTAGCAGTGAAAGTCCATGATAAAGA GACCCCTCAAGACAGTTTGGTGGCCCCTGCCCAGCCGCCTTCTCAGACATTTCCACCTCCCTCCCTCCCC TCAAAAAGTGTTGCAATGCAGCAGAAGCCCACGGCTTATGTCCGGCCCATGGATGGTCAAGATCAGGCCC CTAGTGAATCCCCTGAACTGAAACCACTGCCGGAGGACTATCGACAGCAGACCTTTGAAAAAACAGACTT GAAAGTGCCTGCCAAAGCCAAGCTCACCAAACTGAAGATGCCTTCTCAGTCAGTTGAGCAGACCTACTCC AATGAAGTCCATTGTGTTGAAGAGATTCTGAAGGAAATGACCCATTCATGGCCGCCTCCTTTGACAGCAA TACATACGCCTAGTACAGCTGAGCCATCCAAGTTTCCTTTCCCTACAAAGGACTCTCAGCATGTCAGTTC TGTAACCCAAAACCAAAAACAATATGATACATCTTCAAAAACTCACTCAAATTCTCAGCAAGGAACGTCA TCCATGCTCGAAGACGACCTTCAGCTCAGTGACAGTGAGGACAGTGACAGTGAACAAACCCCAGAGAAGC CTCCCTCCTCATCTGCACCTCCAAGTGCTCCACAGTCCCTTCCAGAACCAGTGGCATCAGCACATTCCAG CAGTGCAGAGTCAGAAAGCACCAGTGACTCAGACAGTTCCTCAGACTCAGAGAGCGAGAGCAGTTCAAGT GACAGCGAAGAAAATGAGCCCCTAGAAACCCCAGCTCCGGAGCCTGAGCCTCCAACAACAAACAAATGGC AGCTGGACAACTGGCTGACCAAAGTCAGCCAGCCAGCTGCGCCACCAGAGGGCCCCAGGAGCACAGAGCC CCCACGGCGGCACCCAGAGAGTAAGGGCAGCAGCGACAGTGCCACGAGTCAGGAGCATTCTGAATCCAAA GATCCTCCCCCTAAAAGCTCCAGCAAAGCCCCCCGGGCCCCACCCGAAGCCCCCCACCCCGGAAAGAGGA GCTGTCAGAAGTCTCCGGCACAGCAGGAGCCCCCACAAAGGCAAACCGTTGGAACCAAACAACCCAAAAA ACCTGTCAAGGCCTCTGCCCGGGCAGGTTCACGGACCAGCCTGCAGGGGGAAAGGGAGCCAGGGCTTCTT CCCTATGGCTCCCGAGACCAGACTTCCAAAGACAAGCCCAAGGTGAAGACGAAAGGACGGCCCCGGGCCG CAGCAAGCAACGAACCCAAGCCAGCAGTGCCCCCCTCCAGTGAGAAGAAGAAGCACAAGAGCTCCCTCCC TGCCCCCTCTAAGGCTCTCTCAGGCCCAGAACCCGCGAAGGACAATGTGGAGGACAGGACCCCTGAGCAC TTTGCTCTTGTTCCCCTGACTGAGAGCCAGGGCCCACCCCACAGTGGCAGCGGCAGCAGGACTAGTGGCT GCCGCCAAGCCGTGGTGGTCCAGGAGGACAGCCGCAAAGACAGACTCCCATTGCCTTTGAGAGACACCAA GCTGCTCTCACCGCTCAGGGACACTCCTCCCCCACAAAGCTTGATGGTGAAGATCACCCTAGACCTGCTC TCTCGGATACCCCAGCCTCCCGGGAAGGGGAGCCGCCAGAGGAAAGCAGAAGATAAACAGCCGCCCGCAG GGAAGAAGCACAGCTCTGAGAAGAGGAGCTCAGACAGCTCAAGCAAGTTGGCCAAAAAGAGAAAGGGTGA AGCAGAAAGAGACTGTGATAACAAGAAAATCAGACTGGAGAAGGAAATCAAATCACAGTCATCTTCATCT TCATCCTCCCACAAAGAATCTTCTAAAACAAAGCCCTCCAGGCCCTCCTCACAGTCCTCAAAGAAGGAAA TGCTCCCCCCGCCACCCGTGTCCTCGTCCTCCCAGAAGCCAGCCAAGCCTGCACTTAAGAGGTCAAGGCG GGAAGCAGACACCTGTGGCCAGGACCCTCCCAAAAGTGCCAGCAGTACCAAGAGCAACCACAAAGACTCT TCCATTCCCAAGCAGAGAAGAGTAGAGGGGAAGGGCTCCAGAAGCTCCTCGGAGCACAAGGGTTCTTCCG GAGATACTGCAAATCCTTTTCCAGTGCCTTCTTTGCCAAATGGTAACTCTAAACCAGGGAAGCCTCAAGT GAAGTTTGACAAACAACAAGCAGACCTTCACATGAGGGAGGCAAAAAAGATGAAGCAGAAAGCAGAGTTA ATGACGGACAGGGTTGGAAAGGCTTTTAAGTACCTGGAAGCCGTCTTGTCCTTCATTGAGTGCGGAATTG CCACAGAGTCTGAAAGCCAGTCATCCAAGTCAGCTTACTCTGTCTACTCAGAAACTGTAGATCTCATTAA ATTCATAATGTCATTAAAATCCTTCTCAGATGCCACAGCGCCAACACAAGAGAAAATATTTGCTGTTTTA TGCATGCGTTGCCAGTCCATTTTGAACATGGCGATGTTTCGTTGTAAAAAAGACATAGCAATAAAGTATT CTCGTACTCTTAATAAACACTTCGAGAGTTCTTCCAAAGTCGCCCAGGCACCTTCTCCATGCATTGCAAG CACAGGCACACCATCCCCTCTTTCCCCAATGCCTTCTCCTGCCAGCTCCGTAGGGTCCCAGTCAAGTGCT GGCAGTGTGGGGAGCAGTGGGGTGGCTGCCACTATCAGCACCCCAGTCACCATCCAGAATATGACATCTT CCTATGTCACCATCACATCCCATGTTCTTACCGCCTTTGACCTTTGGGAACAGGCCGAGGCCCTCACGAG GAAGAATAAAGAATTCTTTGCTCGGCTCAGCACAAATGTGTGCACCTTGGCCCTCAACAGCAGTTTGGTG GACCTGGTGCACTATACACGACAGGGTTTTCAGCAGCTACAAGAATTAACCAAAACACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217054 representing NM_005935
Red=Cloning site Green=Tags(s) MAAQSSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEF LSTKSHTHRLDASENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRT EPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPERELSPLISLPSPVPP LSPIHSNQQTLPRTQGSSKVHGSSNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLP SKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYS NEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTS SMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSS DSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATSQEHSESK DPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRTSLQGEREPGLL PYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNVEDRTPEH FALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLL SRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSS SSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCGQDPPKSASSTKSNHKDS SIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAEL MTDRVGKAFKYLEAVLSFIECGIATESESQSSKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVL CMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIASTGTPSPLSPMPSPASSVGSQSSA GSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLV DLVHYTRQGFQQLQELTKTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005935 |
| ORF Size | 3630 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005935.3 |
| RefSeq Size | 9390 bp |
| RefSeq ORF | 3633 bp |
| Locus ID | 4299 |
| UniProt ID | P51825 |
| Domains | AF-4 |
| Protein Families | Transcription Factors |
| MW | 131.2 kDa |
| Gene Summary | This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217054L1 | Lenti-ORF clone of AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 11,664.00 |
|
| RC217054L2 | Lenti-ORF clone of AFF1 (mGFP-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 13,970.00 |
|
| RC217054L3 | Lenti-ORF clone of AFF1 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 11,664.00 |
|
| RC217054L4 | Lenti-ORF clone of AFF1 (mGFP-tagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 13,970.00 |
|
| RG217054 | AFF1 (tGFP-tagged) - Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 13,300.00 |
|
| SC309949 | AFF1 (untagged)-Human AF4/FMR2 family, member 1 (AFF1), transcript variant 2 |
CNY 20,264.00 |
