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SIK3 (NM_025164) Human Untagged Clone
CAT＃: SC309935
SIK3 (untagged)-Human SIK family kinase 3 (SIK3)
CNY 24,320.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | L19; QSK; SEMDK; SIK-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309935 representing NM_025164.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGCCGGGACTGGGGGAGCCGGGCCCGCG GGCCGCCTGCTGCCTCCGCCCGCGCCGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGC CAGCCGCGTCCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTACGAGATCGAC CGCACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTT GCTATCAAGATCATAGATAAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAA ATTATGAAGATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTATGGAGACAGAACGGATGATT TATCTGGTGACAGAATATGCTAGTGGAGGGGAAATATTTGACCACCTGGTGGCCCATGGTAGAATGGCA GAAAAGGAGGCACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGAACATT GTTCATCGTGATTTAAAAGCTGAAAATTTACTTCTGGATGCCAATCTGAATATCAAAATAGCAGATTTT GGTTTCAGTAACCTCTTCACTCCTGGGCAGCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCA CCTGAACTCTTTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGCCTTGGAGTTGTCCTC TACGTGCTTGTGTGCGGTGCCCTGCCATTTGATGGAAGCACACTGCAGAATCTGCGGGCCCGCGTGCTG AGTGGAAAGTTCCGCATCCCATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTGGTG TTAGATCCCAATAAGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAGCTAGGGGACGCC GATCCCAACTTTGACAGGTTAATAGCTGAATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTG AATGAGGATGTCCTCTTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCAGTCATTAAGA TCAGATGCCTATGATCACTATAGTGCAATCTACAGCCTGCTGTGTGATCGACATAAGAGACATAAAACC CTGCGTCTCGGAGCACTTCCTAGCATGCCCCGAGCCCTGGCCTTTCAAGCACCAGTCAATATCCAGGCG GAGCAGGCAGGTACTGCTATGAACATCAGCGTTCCCCAGGTGCAGCTGATCAACCCAGAGAACCAAATT GTGGAGCCGGATGGGACACTGAATTTGGACAGTGATGAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTG CGCTATTTGTCAATGAGGAGGCACACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTG CAGAAGCTCCTACCTGGCTTTCCTGGAGTCAACCCCCAGGCTCCATTCCTGCAGGTGGCCCCTAATGTG AACTTCATGCACAACCTGTTGCCTATGCAAAACTTGCAACCAACCGGGCAACTTGAGTACAAGGAGCAG TCTCTCCTACAGCCGCCCACGCTACAGCTGTTGAATGGAATGGGCCCCCTTGGCCGGAGGGCATCAGAT GGAGGAGCCAACATCCAACTGCATGCCCAGCAGCTGCTGAAGCGCCCACGGGGACCCTCTCCGCTTGTC ACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAG GAAGCTGTGCAGAGCTCTACCTACAAGGACTCCAACACTCTGCACCTCCCTACGGAGCGTTTCTCCCCT GTGCGCCGGTTCTCAGATGGGGCTGCGAGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAAC AACAGCAGCATCAAACAGCTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGATTGAT GAAAGAACCCTGGAGAAGACCCAGCAGCAGCATATGTTATACCAGCAGGAGCAGCACCATCAAATTCTC CAGCAACAAATTCAAGACTCTATCTGTCCTCCTCAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAAT CAGCCAGCCCTCCTTACCCATCAGCTCCAGAGGTTAAGGATTCAGCCTTCAAGCCCACCCCCCAACCAC CCCAACAACCATCTCTTCAGGCAGCCCAGTAATAGTCCTCCCCCCATGAGCAGTGCCATGATCCAGCCT CACGGGGCTGCATCTTCTTCCCAGTTTCAAGGCTTACCTTCCCGCAGTGCAATCTTTCAGCAGCAACCT GAGAACTGTTCCTCTCCTCCCAACGTGGCACTAACCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACAG CAGGTCACCATCCAAGTCCAAGAGCCTGTTGACATGCTCAGCAACATGCCAGGCACAGCTGCAGGCTCC AGTGGGCGCGGCATCTCCATCAGCCCCAGTGCTGGTCAGATGCAGATGCAGCACCGTACCAACCTGATG GCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGCAGCTGAGTGCTGACAGTGCAGAGGCTCACAGC TTGAACGTGAATCGGTTCTCCCCTGCTAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGAC CAGTCCCGGGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTCTCTCCAACCCAAGCCCTG AAAGTCCCTCCACTTGACCAATTCCCCACCTTCCCTCCCAGTGCACATCAGCAGCCGCCACACTATACC ACGTCGGCACTACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGTACCC CACATCCTTCAAGGACTGCTTTCTCCCCGGCATTCGCTCACCGGCCACTCGGACATCCGGCTGCCCCCA ACAGAGTTTGCACAGCTCATTAAAAGGCAGCAGCAACAACGGCAGCAGCAGCAGCAACAGCAGCAACAG CAAGAATACCAGGAACTGTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTCCCAGCCTTGGG GGACAGAGCATGACAGAGCGCCAGGCTTTATCTTATCAAAATGCTGACTCTTATCACCATCACACCAGC CCCCAGCATCTGCTACAAATCAGGGCACAAGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCCAC GGGTATGCTCACCAGCCGGCACTGATGCATTCAGAGAGCATGGAGGAGGACTGCTCGTGTGAGGGGGCC AAGGATGGCTTCCAAGACAGTAAGAGTTCAAGTACATTGACCAAAGGTTGCCATGACAGCCCTCTGCTC TTGAGTACCGGTGGACCTGGGGACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCCCAAGAATTGGGG ATACATCCCTATGGTCATCAGCCAACTGCTGCATTCAGTAAAAATAAGGTGCCCAGCAGAGAGCCTGTC ATAGGGAACTGCATGGATAGAAGTTCTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGG TACCCAGCACGCCCCTCCGTCCATGAGCACCACAGGCCCCGGGCCCTCCAGAGACACCACACGATCCAG AACAGCGACGATGCTTATGTACAGCTGGATAACTTGCCAGGAATGAGTCTCGTGGCTGGGAAAGCACTT AGCTCTGCCCGGATGTCGGATGCAGTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGAT GGGGAAAATGAGGAATGTGGGGCAAGCCTGGGAGGTCATGAGCACCCAGACCTGAGTGATGGCAGCCAG CATTTAAACTCCTCTTGCTATCCATCTACGTGTATTACAGACATTCTGCTCAGCTACAAGCACCCCGAA GTCTCCTTCAGCATGGAGCAGGCAGGCGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_025164 |
| Insert Size | 3966 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_025164.4 |
| RefSeq Size | 6219 bp |
| RefSeq ORF | 3966 bp |
| Locus ID | 23387 |
| UniProt ID | Q9Y2K2 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 144.9 kDa |
| Gene Summary | Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed:30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223406 | SIK3 (Myc-DDK-tagged)-Human SIK family kinase 3 (SIK3) |
CNY 8,792.00 |
|
| RC223406L1 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), Myc-DDK-tagged |
CNY 11,192.00 |
|
| RC223406L2 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), mGFP tagged |
CNY 14,540.00 |
|
| RC223406L3 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), Myc-DDK-tagged |
CNY 11,192.00 |
|
| RC223406L4 | Lenti ORF clone of Human SIK family kinase 3 (SIK3), mGFP tagged |
CNY 11,192.00 |
|
| RG223406 | SIK3 (tGFP-tagged) - Human SIK family kinase 3 (SIK3) |
CNY 13,870.00 |
