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MTR (NM_000254) Human Untagged Clone
CAT＃: SC309934
MTR (untagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR)
CNY 13,224.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cblG; HMAG; MS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_000254 edited
ACGTCGTCCTCCTCTGCCGGTTTTCTCTTGGGTCCTTTTCCGTGCCGTCCCGCGACTCCG CCTCTGGCCGCGCGTGTCTGGCTGCTAGGCCGACACCAAGGACTGGCCGGGTACCCGGGA AGAAAGCACGTGCTCCAGCAGTTGCCGCGCCCAGCCCCGAGAGAGGCCCTAGGGCGCTGC GGGCTTTCGGGGTCCGCAGTCCCCCCGCGACGCGAGCCAACGGGAGGCGTCAAAAGACCC GGGCCTTGTGTGGCAGGCTCGCCTGGCGCTGGCTGGCGTGGCCCTTGGCCGTCGTCACCT GTGGAGAGCACGTCTTCTCTGCCGCGCCCTCTGCGCAAGGAGGAGACTCGACAACATGTC ACCCGCGCTCCAAGACCTGTCGCAACCCGAAGGTCTGAAGAAAACCCTGCGGGATGAGAT CAATGCCATTCTGCAGAAGAGGATTATGGTGCTGGATGGAGGGATGGGGACCATGATCCA GCGGGAGAAGCTAAACGAAGAACACTTCCGAGGTCAGGAATTTAAAGATCATGCCAGGCC GCTGAAAGGCAACAATGACATTTTAAGTATAACTCAGCCTGATGTCATTTACCAAATCCA TAAGGAATACTTGCTGGCTGGGGCAGATATCATTGAAACAAATACTTTTAGCAGCACTAG TATTGCCCAAGCTGACTATGGCCTTGAACACTTGGCCTACCGGATGAACATGTGCTCTGC AGGAGTGGCCAGAAAAGCTGCCGAGGAGGTAACTCTCCAGACAGGAATTAAGAGGTTTGT GGCAGGGGCTCTGGGTCCGACTAATAAGACACTCTCTGTGTCCCCATCTGTGGAAAGGCC GGATTATAGGAACATCACATTTGATGAGCTTGTTGAAGCATACCAAGAGCAGGCCAAAGG ACTTCTGGATGGCGGGGTTGATATCTTACTCATTGAAACTATTTTTGATACTGCCAATGC CAAGGCAGCCTTGTTTGCACTCCAAAATCTTTTTGAGGAGAAATATGCTCCCCGGCCTAT CTTTATTTCAGGGACGATCGTTGATAAAAGTGGGCGGACTCTTTCCGGACAGACAGGAGA GGGATTTGTCATCAGCGTGTCTCATGGAGAACCACTCTGCATTGGATTAAATTGTGCTTT GGGTGCAGCTGAAATGAGACCTTTTATTGAAATAATTGGAAAATGTACAACAGCCTATGT CCTCTGTTATCCCAATGCAGGTCTTCCCAACACCTTTGGTGACTATGATGAAACGCCTTC TATGATGGCCAAGCACCTAAAGGATTTTGCTATGGATGGCTTGGTCAATATAGTTGGAGG ATGCTGTGGGTCAACACCAGATCATATCAGGGAAATTGCTGAAGCTGTGAAAAATTGTAA GCCTAGAGTTCCACCTGCCACTGCTTTTGAAGGACATATGTTACTGTCTGGTCTAGAGCC CTTCAGGATTGGACCGTACACCAACTTTGTTAACATTGGAGAGCGCTGTAATGTTGCAGG ATCAAGGAAGTTTGCTAAACTCATCATGGCAGGAAACTATGAAGAAGCCTTGTGTGTTGC CAAAGTGCAGGTGGAAATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGCATGCT AGATGGTCCAAGTGCAATGACCAGATTTTGCAACTTAATTGCTTCCGAGCCAGACATCGC AAAGGTACCTTTGTGCATCGACTCCTCCAATTTTGCTGTGATTGAAGCTGGGTTAAAGTG CTGCCAAGGGAAGTGCATTGTCAATAGCATTAGTCTGAAGGAAGGAGAGGACGACTTCTT GGAGAAGGCCAGGAAGATTAAAAAGTATGGAGCTGCTATGGTGGTCATGGCTTTTGATGA AGAAGGACAGGCAACAGAAACAGACACAAAAATCAGAGTGTGCACCCGGGCCTACCATCT GCTTGTGAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGACCCTAATATCCTAAC CATTGGGACTGGAATGGAGGAACACAACTTGTATGCCATTAATTTTATCCATGCAACAAA AGTCATTAAAGAAACATTACCTGGAGCCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTT CTCCTTCCGAGGAATGGAAGCCATTCGAGAAGCAATGCATGGGGTTTTCCTTTACCATGC AATCAAGTCTGGCATGGACATGGGGATAGTGAATGCTGGAAACCTCCCTGTGTATGATGA TATCCATAAGGAACTTCTGCAGCTCTGTGAAGATCTCATCTGGAATAAAGACCCTGAGGC CACTGAGAAGCTCTTACGTTATGCCCAGACTCAAGGCACAGGAGGGAAGAAAGTCATTCA GACTGATGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTATGCCCTTGTGAAGGG CATTGAAAAACATATTATTGAGGATACTGAGGAAGCCAGGTTAAACCAAAAAAAATATCC CCGACCTCTCAATATAATTGAAGGACCCCTGATGAATGGAATGAAAATTGTTGGTGATCT TTTTGGAGCTGGAAAAATGTTTCTACCTCAGGTTATAAAGTCAGCCCGGGTTATGAAGAA GGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAAACCAGAGTGCTTAA CGGCACAGTAGAAGAAGAGGACCCTTACCAGGGCACCATCGTGCTGGCCACTGTTAAAGG CGACGTGCACGACATAGGCCAGAACATAGTTGGAGTAGTCCTTGGCTGCAATAATTTCCG AGTTATTGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAAAGCTGCTCTTGACCA CAAAGCAGATATAATTGGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATGATTTT TGTTGCCAAGGAAATGGAGAGATTAGCTATAAGGATTCCATTGTTGATTGGAGGAGCAAC CACTTCAAAAACCCACACAGCAGTTAAAATAGCTCCGAGATACAGTGCACCTGTAATCCA TGTCCTGGACGCGTCCAAGAGTGTGGTGGTGTGTTCCCAGCTGTTAGATGAAAATCTAAA GGATGAATACTTTGAGGAAATCATGGAAGAATATGAAGATATTAGACAGGACCATTATGA GTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTCAAGCCAGAAAAAGTGGTTTCCAAAT GGATTGGCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGA AGACTATGACCTGCAGAAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTG GCAGCTCCGGGGCAAGTACCCGAATCGAGGCTTTCCCAAGATATTTAACGACAAAACAGT AGGTGGAGAGGCCAGGAAGGTCTACGATGATGCCCACAATATGCTGAACACACTGATTAG TCAAAAGAAACTCCGGGCCCGGGGTGTGGTTGGGTTCTGGCCAGCACAGAGTATCCAAGA CGACATTCACCTGTACGCAGAGGCTGCTGTGCCCCAGGCTGCAGAGCCCATAGCCACCTT CTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTGCCAGCACGGAGCCATACTACTGCCT CTCAGACTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCTGGGCCTGTTTGCCGT TGCCTGCTTTGGGGTAGAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGACTACAG CAGCATCATGGTCAAGGCGCTGGGGGACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCA TGAAAGAGTTCGCCGAGAACTGTGGGCCTACTGTGGCAGTGAGCAGCTGGACGTCGCAGA CCTGCGCAGGCTGCGGTACAAGGGCATCCGCCCGGCTCCTGGCTACCCCAGCCAGCCCGA CCACACCGAGAAGCTCACCATGTGGAGACTCGCAGACATCGAGCAGTCTACAGGCATTAG GTTAACAGAATCATTAGCAATGGCACCTGCTTCAGCAGTCTCAGGCCTCTACTTCTCCAA TTTGAAGTCCAAATATTTTGCTGTGGGGAAGATTTCCAAGGATCAGGTTGAGGATTATGC ATTGAGGAAGAACATATCTGTGGCTGAGGTTGAGAAATGGCTTGGACCCATTTTGGGATA TGATACAGACTAACTTTTTTTTTTTTTTTGCCTTTTTTATTCTTGATGATCCTCAAGGAA ATACAACCTAGGGTGCCTTAAAAATAACAACAACAAAAAACCTGTGTGCATCTGGCTGAC ACTTACCTGCTTCTGGTTTTCGAAGACTATTTAGTGGAACCTTGTAGAGGAGCAGGGTCT TCCTGCAGTGCCTGGAAAACAGGCGCTGTTTTTTTTTGGGACCTTGCGTGAAGAGCAGTG AGCAGGGTTCCTGTGGTTTCCCTGGTCCCTCTGAGATGGGGACAGACTGAAGACAGAGGT CGTTTGATTTCAAAGCAAGTCAACCTGCTTTTTTCTGTTTTTACAGTGGAATCTAGGAGG CCACTTAGTCGTCTTTTTTTCCTCTTAGAAGAAAAGCCTGAAACTGAGTTGAATAGAGAA GTGTGACCCTGTGACAAAATGATACTGTGAGAAATGGGGCATTTTAATCTAAGTGGTTAT AACAGTGGATTCTGACGGGGAAGGTGTAGCTCTGTTCTCTTCGGAAGACCTCGTTTTCTA AAGGCTGGACTAAATGGCTGCAGAACTCCCTTTGGCAAAAGGCATGCGCTCACTGCTTGC TTGTCAGAAACACTGAAGCCATTTGCCCCAGTGTGGTCAAGCAGCCATGCTTTCTGGGCA TTTTCGTCCTCCCATAATTTCATATTTCCGTACCCCTGAGGAAACAAAAAGGAAATGAGG AGAGAAAGTTACTGTTAAGGGTGGTTAACATTTTTTTTGTTTTGTTTTGTTTTGGTTTTT TTTTTTTGAGACAGAGTCTGGCTCTGTCGCCCAGGCTGGAGTGCAGGGGCGCAATCTCGG CTCATAGCAAGCTCCGCCTCCTGGGTTCATGCCATTCTCCTGCCTCAGCCTCCAGAGTAG CTGGGACTACAGGTGCCCACCACCACACCCGGCTAATTTTTTGTGTTTTTACAAAATACA AAAAAGTAGAGACAGGATTTCACTGTGTTAGCCAGGATGGTCTTGATCTCCCGACCTCGT GATCTGCCCACCTCAGCCTCCCAAAATGCTGGGATTACAGGCGTGAGCCACCGAGCCTGG CCGGTTAACATCTTTTAATTGTTTCCAGGATTGAGCAGGTTCTCAGCTGGGCTCTGATAT CCCGTGCGGAGTTGGACAAGTGGGCAGCATAAAGTCACTCATTTCTTACCATTTTATTCC CCTCAATTCTCAATATATTCAGTAATGAAGAATGGTGCCACCACTCAAGCAACAAGCCTC AAACTCAACCATGTCATCTTTTTCTTGGATGATTGCAGTTATTTCAAAAATTTGCATGCA AAATATACACTCATCCTACTTCAAGATGGTGGTGGCAATAGTCAGGAGAAGGTAACATTG GAGTCCTGGTTTGATTCGAAGGATGAAGACGAAGAAGCAAGGGAGGAACAAATGAAGAAC CATCTTTGTTCATGAATAGGAATATTCAAGATTATAAAGGTATCAGGTCTCCTAAAATTG ATCTATGGATTTAATACCATTTTCAATGGAAATTCCAACAGATTTTATTGAATGAAACAA GCAGGTGTTTATATGGAGTAGCAAAGGACTTAAAATTACCAAATGCTTCTAAATATGAAG GAGAGGTTGGGGACACGCACCCTATGTGATACCAAGTTTTATTGTCAAGACAGTGTCATG GTGCAGAGGTAGGCATTCTGAGCAGGGGAACAAAATAAGGGCCTAGAAACTCACCCGTGC ATATGTTGACCTTTGCAAAATGACCTGGTGACATGGCAAGTCAGTGGGGACAGGAAGGAC CACTCCCTAAGTAATCCCAGAACAATGGCTATTCATGTGGGAAAAAAAGAAATTTTACTT TCTCTCACCTTACCTGGTGATAAGTTCCAAATATGTTAAGGGCTTTAATACAAAAAGCAA AAATTGTCAGTGTTTGGATGAAAAAAGCCTTAGGGCAGGAAAGAATCTCTTGAGACATAA AGTAGTAATCATAAAGGACAAGATGGTTAAGTCAATTCTGTTAAAACTCAAGGCTTATAT TAAGCAAACACTTGAAGTGAGAAGATGATCCACAACTTGAGAAGACATTTATAATACAAA TAACTGATGAAGGATTCATAATCACAAATATAGAGAATTCCTATTTAAAAAAATAGAAAA ATAGTGAAGACTACACAAGAGGAAATAGGGCTTTTAAATAAATAGATGTTCTGTAGCATT GGTCAGGGAAATATGAATTAGGACCACAATGAGATTCCATTTTATATCCATAAGATTTGC AAAGGTTGGGTCTGACAGTACCAGTTGTTAGATCTGTAGGGACTTGTACAACATTGTGGA TGTGTAAACAGGCACCACTGCTTTAAAAAACAATTATCCCTTACAGACTTGAACATTTGC AGACGTTATGATCTTGCTTCCAACTCCCACCTGTATGTCCAGCAAACTCTTGCATGTGGC CACTAGGAGGAATGTGTAAGAATGTTCATAGTTACATATTTATAATAGTTAATAACTGGA AAAAGTGAAATGTATGTCTGTCTACAGGAAAATAGGTGAATAATTAGATATATATATTCA TTCTACGGGATATTATTCAGTAGTGGAAATGAGTGAACTACAGCTATACCTCACAATAAG AATGAATCTCAGAAAATATTAAGGAAAAAAGCAAGTTTGAAGAGACCACATGGGGCGTAC TATTTTTATTGAGCCCAAAAACAAGCAAAACCAAAGAATATGTAGTCTAAGCATACGTAT ACAATAAAACTATGCTATTAAAAAAAAAAGGTAACTGATAAACCAAAATTGAGCATAGTA ATTACCCACAGAAGGAGGAAGTGGAAGGGACAGGAGCACATAGGTAGATGCCAAGTTATG CAGCTGTTCTGGTTCCTCCTGGTAGGCTTACAAGTGTTTACTATATGCTATTAATACATT ATACTTTATAACTAATAGATAACAGTTTTTTACATATTAAATATGTTCTACTTAAATATA TTATAAAAAATAAAGGCAAAGTGGAATGAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_000254 |
| Insert Size | 7200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by two amino acids. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000254.1, NP_000245.1 |
| RefSeq Size | 7122 bp |
| RefSeq ORF | 3798 bp |
| Locus ID | 4548 |
| UniProt ID | Q99707 |
| Domains | Pterin_bind, S-methyl_trans, B12-binding_2, Met_synt_B12, B12-binding |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate |
| Gene Summary | This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214275 | MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,088.00 |
|
| RC214275L1 | Lenti-ORF clone of MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RC214275L2 | Lenti-ORF clone of MTR (mGFP-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RC214275L3 | Lenti-ORF clone of MTR (Myc-DDK-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RC214275L4 | Lenti-ORF clone of MTR (mGFP-tagged)-Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 14,540.00 |
|
| RG214275 | MTR (tGFP-tagged) - Human 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR) |
CNY 13,870.00 |
