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Myoferlin (MYOF) (NM_013451) Human Untagged Clone
CAT＃: SC309765
MYOF (untagged)-Human myoferlin (MYOF), transcript variant 1
CNY 39,620.00
货期*
4周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FER1L3; HAE7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309765 representing NM_013451.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCGAGTGATTGTGGAATCTGCCAGCAATATCCCTAAAACGAAATTTGGCAAGCCGGATCCTATT GTTTCTGTCATTTTTAAGGATGAGAAAAAGAAAACAAAGAAAGTTGATAATGAATTGAACCCTGTCTGG AATGAGATTTTGGAGTTTGACTTGAGGGGTATACCACTGGACTTTTCATCTTCCCTTGGGATTATTGTG AAAGATTTTGAGACAATTGGACAAAATAAATTAATTGGCACGGCGACTGTAGCCCTGAAGGACCTGACT GGTGACCAGAGCAGATCCCTGCCGTACAAGCTGATCTCCCTGCTAAATGAAAAAGGGCAAGATACTGGG GCCACCATTGACTTGGTGATCGGCTATGATCCGCCTTCTGCTCCACATCCAAATGACCTGAGCGGGCCC AGCGTGCCAGGCATGGGAGGAGATGGGGAAGAAGATGAAGGTGATGAAGACAGGTTGGACAATGCAGTC AGGGGCCCTGGGCCCAAGGGGCCAGTTGGGACGGTGTCGGAAGCTCAGCTTGCTCGGAGGCTCACCAAA GTAAAGAACAGCCGGCGGATGCTGTCAAATAAGCCACAGGACTTCCAGATCCGCGTCCGAGTGATTGAG GGCCGACAGTTAAGTGGTAACAACATAAGGCCTGTGGTCAAAGTTCACGTCTGTGGCCAGACACACCGA ACAAGAATCAAGAGAGGAAACAACCCTTTTTTTGATGAGTTGTTTTTCTACAATGTCAACATGACCCCT TCTGAATTGATGGATGAGATCATCAGCATCCGGGTTTATAATTCTCACTCTCTGCGGGCAGATTGTCTG ATGGGGGAATTTAAGATTGATGTTGGATTTGTTTATGATGAACCTGGCCATGCTGTCATGAGAAAGTGG CTTCTTCTCAATGACCCGGAAGATACCAGTTCAGGTTCTAAAGGTTATATGAAAGTCAGCATGTTTGTC CTGGGAACCGGAGATGAGCCTCCTCCTGAGAGACGAGATCGTGATAATGACAGTGATGATGTGGAGAGT AATTTGTTACTCCCTGCTGGCATTGCCCTCCGGTGGGTGACCTTCTTGCTGAAAATCTACCGAGCTGAG GACATCCCCCAGATGGATGATGCCTTCTCACAGACAGTAAAGGAAATATTTGGAGGCAATGCAGATAAG AAAAATCTCGTGGATCCTTTTGTAGAAGTTTCCTTTGCTGGAAAAAAGGTTTGTACAAACATAATTGAG AAAAATGCAAACCCAGAGTGGAATCAGGTCGTCAATCTTCAGATCAAGTTTCCTTCAGTGTGTGAAAAA ATAAAACTAACAATATATGACTGGGACCGTCTTACTAAAAATGATGTAGTTGGAACAACATATCTACAC CTCTCTAAAATTGCTGCCTCTGGTGGGGAAGTGGAAGATTTCTCATCTTCGGGAACTGGGGCTGCATCA TATACAGTAAACACAGGAGAAACAGAGGTAGGCTTTGTTCCAACGTTTGGACCTTGTTACCTGAATCTT TATGGAAGCCCCAGAGAGTACACGGGATTCCCAGACCCCTATGATGAGCTGAATACTGGAAAGGGGGAA GGAGTTGCCTACAGAGGCAGGATCTTGGTTGAATTAGCCACTTTTCTTGAGAAGACACCACCAGATAAA AAGCTTGAGCCCATTTCAAATGATGACCTGCTGGTTGTTGAGAAATACCAGCGAAGGCGGAAGTACAGC CTGTCTGCCGTGTTTCATTCAGCCACCATGTTGCAAGATGTTGGTGAGGCCATTCAGTTTGAAGTCAGC ATTGGGAACTATGGCAACAAGTTTGACACCACCTGTAAGCCTTTGGCATCAACAACTCAGTACAGCCGT GCTGTATTTGATGGCAACTACTATTATTACTTGCCTTGGGCCCACACCAAGCCAGTTGTTACCCTGACT TCATACTGGGAGGATATTAGTCATCGCCTGGATGCGGTGAACACTCTCCTAGCTATGGCAGAACGGCTG CAAACAAATATAGAAGCTCTAAAATCAGGGATACAAGGTAAAATTCCTGCAAACCAGCTGGCTGAATTG TGGCTGAAGCTGATAGATGAAGTTATAGAAGACACGAGATACACGTTGCCTCTCACAGAAGGAAAAGCC AACGTCACAGTTCTCGATACTCAGATCCGAAAGCTGCGGTCCAGGTCTCTCTCCCAAATACATGAGGCG GCTGTGAGGATGAGGTCGGAAGCCACAGATGTGAAGTCCACACTGGCAGAAATTGAGGACTGGCTTGAT AAATTAATGCAGCTGACTGAAGAGCCACAGAACAGCATGCCTGACATCATCATCTGGATGATCCGGGGA GAGAAGAGACTGGCCTATGCACGAATTCCCGCACATCAGGTCTTGTACTCCACCAGTGGTGAGAATGCA TCTGGAAAATACTGTGGGAAAACCCAAACCATCTTTCTGAAGTATCCACAGGAGAAAAACAACGGGCCA AAGGTGCCTGTGGAGTTGCGAGTGAACATCTGGCTAGGCTTAAGTGCTGTGGAGAAGAAGTTTAACAGC TTCGCAGAAGGAACTTTCACCGTCTTTGCTGAAATGTATGAAAATCAAGCTCTCATGTTTGGAAAATGG GGTACTTCTGGATTAGTAGGACGTCATAAGTTTTCTGATGTCACAGGAAAAATAAAACTCAAGAGGGAA TTTTTTCTGCCTCCAAAAGGCTGGGAATGGGAAGGAGAGTGGATAGTTGATCCTGAAAGAAGCTTGCTG ACTGAGGCAGATGCAGGTCACACGGAGTTCACTGATGAAGTCTATCAGAACGAGAGCCGCTACCCCGGG GGCGACTGGAAGCCGGCCGAGGACACCTACACGGATGCGAACGGCGATAAAGCAGCATCACCCAGCGAG TTGACTTGTCCTCCAGGTTGGGAATGGGAAGATGATGCATGGTCTTATGACATAAATCGAGCGGTGGAT GAGAAAGGCTGGGAATATGGAATCACCATTCCTCCTGATCATAAGCCCAAATCCTGGGTTGCAGCAGAG AAAATGTACCACACTCATAGACGGCGAAGGCTGGTCCGAAAACGCAAGAAAGATTTAACACAGACTGCT TCAAGCACCGCAAGGGCCATGGAGGAATTGCAAGACCAAGAGGGCTGGGAATATGCTTCTCTAATTGGC TGGAAATTTCACTGGAAACAACGTAGTTCAGATACCTTCCGCCGCAGACGCTGGAGGAGAAAAATGGCT CCTTCAGAAACACATGGTGCAGCTGCCATCTTTAAACTTGAAGGTGCCCTTGGGGCAGACACTACCGAA GATGGGGATGAGAAGAGCCTGGAGAAACAGAAGCACAGTGCCACCACTGTGTTCGGAGCAAACACCCCC ATTGTTTCCTGCAATTTTGACAGAGTCTACATCTACCATCTGCGCTGCTATGTCTATCAAGCCAGAAAC CTCTTGGCTTTAGATAAGGATAGCTTTTCAGATCCATATGCTCATATCTGTTTCCTCCATCGGAGCAAA ACCACTGAGATCATCCATTCAACCCTGAATCCCACGTGGGACCAAACAATTATATTCGATGAAGTTGAA ATCTATGGGGAACCCCAAACAGTTCTACAGAATCCACCCAAAGTTATCATGGAACTTTTTGACAATGAC CAAGTGGGCAAAGATGAATTTTTAGGACGAAGCATTTTCTCTCCTGTGGTGAAACTGAACTCAGAAATG GACATCACACCCAAACTTCTCTGGCACCCAGTAATGAATGGAGACAAAGCCTGCGGGGATGTTCTTGTA ACTGCAGAGCTGATTCTGAGGGGCAAGGATGGCTCCAACCTTCCCATTCTTCCCCCTCAAAGGGCGCCA AATCTATACATGGTCCCCCAGGGGATCAGGCCTGTGGTCCAGCTCACTGCCATTGAGATTCTAGCTTGG GGCTTAAGAAATATGAAAAACTTCCAGATGGCTTCTATCACATCCCCCAGTCTTGTTGTGGAGTGTGGA GGAGAAAGGGTGGAATCGGTGGTGATCAAAAACCTTAAGAAGACACCCAACTTTCCAAGTTCTGTTCTC TTCATGAAAGTGTTCTTGCCCAAGGAGGAATTGTACATGCCCCCACTGGTGATCAAGGTCATCGACCAC AGGCAGTTTGGGCGGAAGCCTGTCGTCGGCCAGTGCACCATCGAGCGCCTGGACCGCTTTCGCTGTGAC CCTTATGCAGGGAAAGAGGACATCGTCCCACAGCTCAAAGCCTCCCTTCTGTCTGCCCCACCATGCCGG GACATCGTTATCGAAATGGAAGACACCAAACCATTACTGGCTTCTAAGCTGACAGAAAAGGAGGAAGAA ATCGTGGACTGGTGGAGTAAATTTTATGCTTCCTCAGGGGAACATGAAAAATGCGGACAGTATATTCAG AAAGGCTATTCCAAGCTCAAGATATATAATTGTGAACTAGAAAATGTAGCAGAATTTGAGGGCCTGACA GACTTCTCAGATACGTTCAAGTTGTACCGAGGCAAGTCGGATGAAAATGAAGATCCTTCTGTGGTTGGA GAGTTTAAGGGCTCCTTTCGGATCTACCCTCTGCCGGATGACCCCAGCGTGCCAGCCCCTCCCAGACAG TTTCGGGAATTACCTGACAGCGTCCCACAGGAATGCACGGTTAGGATTTACATTGTTCGAGGCTTAGAG CTCCAGCCCCAGGACAACAATGGCCTGTGTGACCCTTACATAAAAATAACACTGGGCAAAAAAGTCATT GAAGACCGAGATCACTACATTCCCAACACTCTCAACCCAGTCTTTGGCAGGATGTACGAACTGAGCTGC TACTTACCTCAAGAAAAAGACCTGAAAATTTCTGTCTATGATTATGACACCTTTACCCGGGATGAAAAA GTAGGAGAAACAATTATTGATCTGGAAAACCGATTCCTTTCCCGCTTTGGGTCCCACTGCGGCATACCA GAGGAGTACTGTGTTTCTGGAGTCAATACCTGGCGAGATCAACTGAGACCAACACAGCTGCTTCAAAAT GTCGCCAGATTCAAAGGCTTCCCACAACCCATCCTTTCCGAAGATGGGAGTAGAATCAGATATGGAGGA CGAGACTACAGCTTGGATGAATTTGAAGCCAACAAAATCCTGCACCAGCACCTCGGGGCCCCTGAAGAG CGGCTTGCTCTTCACATCCTCAGGACTCAGGGGCTGGTCCCTGAGCACGTGGAAACAAGGACTTTGCAC AGCACCTTCCAGCCCAACATTTCCCAGGGAAAACTTCAGATGTGGGTGGATGTTTTCCCCAAGAGTTTG GGGCCACCAGGCCCTCCTTTCAACATCACACCCCGGAAAGCCAAGAAATACTACCTGCGTGTGATCATC TGGAACACCAAGGACGTTATCTTGGACGAGAAAAGCATCACAGGAGAGGAAATGAGTGACATCTACGTC AAAGGCTGGATTCCTGGCAATGAAGAAAACAAACAGAAAACAGATGTCCATTACAGATCTTTGGATGGT GAAGGGAATTTTAACTGGCGATTTGTTTTCCCGTTTGACTACCTTCCAGCCGAACAACTCTGTATCGTT GCGAAAAAAGAGCATTTCTGGAGTATTGACCAAACGGAATTTCGAATCCCACCCAGGCTGATCATTCAG ATATGGGACAATGACAAGTTTTCTCTGGATGACTACTTGGGTTTCCTAGAACTTGACTTGCGTCACACG ATCATTCCTGCAAAATCACCAGAGAAATGCAGATTGGACATGATTCCGGACCTCAAAGCCATGAACCCC CTTAAAGCCAAGACAGCCTCCCTCTTTGAGCAGAAGTCCATGAAAGGATGGTGGCCATGCTACGCAGAG AAAGATGGCGCCCGCGTAATGGCTGGGAAAGTGGAGATGACATTGGAAATCCTCAACGAGAAGGAGGCC GACGAGAGGCCAGCCGGGAAGGGGCGGGACGAACCCAACATGAACCCCAAGCTGGACTTACCAAATCGA CCAGAAACCTCCTTCCTCTGGTTCACCAACCCATGCAAGACCATGAAGTTCATCGTGTGGCGCCGCTTT AAGTGGGTCATCATCGGCTTGCTGTTCCTGCTTATCCTGCTGCTCTTCGTGGCCGTGCTCCTCTACTCT TTGCCGAACTATTTGTCAATGAAGATTGTAAAGCCAAATGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013451 |
| Insert Size | 6186 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013451.3 |
| RefSeq Size | 6860 bp |
| RefSeq ORF | 6186 bp |
| Locus ID | 26509 |
| UniProt ID | Q9NZM1 |
| Domains | C2, DysFN, DysFC |
| Protein Families | Transmembrane |
| MW | 234.7 kDa |
| Gene Summary | Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008] Transcript Variant: This variant (1) encodes the full-length isoform (a). |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218766 | MYOF (Myc-DDK-tagged)-Human myoferlin (MYOF), transcript variant 1 |
CNY 13,608.00 |
|
| RC218766L1 | Lenti ORF clone of Human myoferlin (MYOF), transcript variant 1, Myc-DDK-tagged |
CNY 16,008.00 |
|
| RG218766 | MYOF (tGFP-tagged) - Human myoferlin (MYOF), transcript variant 1 |
CNY 20,900.00 |
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