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HCAR2 (NM_177551) Human Untagged Clone
CAT＃: SC309249
HCAR2 (untagged)-Human G protein-coupled receptor 109A (GPR109A)
CNY 3,656.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPR109A; HCA2; HM74a; HM74b; NIACR1; Puma-g; PUMAG |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_177551 edited
CACCTCCTTGCTGGAGCATTCACTAGGCGAGGCGCTCCATCGGACTCACTAGCCGCACTC ATGAATCGGCACCATCTGCAGGATCACTTTCTGGAAATAGACAAGAAGAACTGCTGCGTG TTCCGAGATGACTTCATTGTCAAGGTGTTGCCGCCGGTGTTGGGGCTGGAGTTTATCTTC GGGCTTCTGGGCAATGGCCTTGCCCTGTGGATTTTCTGTTTCCACCTCAAGTCCTGGAAA TCCAGCCGGATTTTCCTGTTCAACCTGGCAGTGGCTGACTTTCTACTGATCATCTGCCTG CCCTTCCTGATGGACAACTATGTGAGGCGTTGGGACTGGAAGTTTGGGGACATCCCTTGC CGGCTGATGCTCTTCATGTTGGCTATGAACCGCCAGGGCAGCATCATCTTCCTCACGGTG GTGGCGGTAGACAGGTATTTCCGGGTGGTCCATCCCCACCACGCCCTGAACAAGATCTCC AATCGGACAGCAGCCATCATCTCTTGCCTTCTGTGGGGCATCACTATTGGCCTGACAGTC CACCTCCTGAAGAAGAAGATGCCGATCCAGAATGGCGGTGCAAATTTGTGCAGCAGCTTC AGCATCTGCCATACCTTCCAGTGGCACGAAGCCATGTTCCTCCTGGAGTTCTTCCTGCCC CTGGGCATCATCCTGTTCTGCTCAGCCAGAATTATCTGGAGCCTGCGGCAGAGACAAATG GACCGGCATGCCAAGATCAAGAGAGCCATCACCTTCATCATGGTGGTGGCCATCGTCTTT GTCATCTGCTTCCTTCCCAGCGTGGTTGTGCGGATCCGCATCTTCTGGCTCCTGCACACT TCGGGCACGCAGAATTGTGAAGTGTACCGCTCGGTGGACCTGGCGTTCTTTATCACTCTC AGCTTCACCTACATGAACAGCATGCTGGACCCCGTGGTGTACTACTTCTCCAGCCCATCC TTTCCCAACTTCTTCTCCACTTTGATCAACCGCTGCCTCCAGAGGAAGATGACAGGTGAG CCAGATAATAACCGCAGCACGAGCGTCGAGCTCACAGGGGACCCCAACAAAACCAGAGGC GCTCCAGAGGCGTTAATGGCCAACTCCGGTGAGCCATGGAGCCCCTCTTATCTGGGCCCA ACCTCTCCTTAAATAACCATGCCAAGAAGGGACATTGTCACCAAGAACCAGGATCTCTGG AGAAACAGTTGGGCTGTTGCATCGAGTAATGTCACTGGACTCGGCCTAAGGTTTCCTGGA ACTTCCAGATTCAGAGAATCTGATTTAGGGAAACTGTGGCAGATGAGTGGGAGACTGGTT GCAAGGTGTGACCGCAGGAATCCTGGAGGAACAGAGAGTAAAGCTTCTAGGCATCTGAAA CTTTTGCTTCATCTCTGACGCTCGCAGGACTGAAGATGGGCAAATTGTAGGCGTTTCTGC TGAGCAGAGTTGGAGCCAGAGATCTACTTGTGACTTGTTGGCCTTCTTCCCACATCTGCC TCAGACTGGGGGGGGCTCAGCTCCTGGGGTGATATCTAGCCTGCTTGTGAGCTCTAGCAG GGATAAGGAGAGCTGAGATTGGAGGGAATTGTGTTGCTCCTGGAGGGAGCCCAGGCATCA TTAAACAAGCCAGTAGGTCACCTGGCTTCCGTCGACCAATTCATCTTTCAGACAAGCTTT AGCAGAAATGGACTCAGGGAAGAGACTCACACGCTTTGGTTAATATCTGTGTTTCCGGTG GGTGTAATAGGGGATTAGCCCCAGAAGGGACTGAGCTAAACAGTGTTATTATGGGAAAGG AAATGGCATTGCTGCTTTCAACCAGCGACTAATGCAATCCATTCCTCTCTTGTTTATAGT AATCTAAGGGTTGGGCAGTTAAAACGGCTTCAGGATAGAAAGCTGTTTCCCACCTCTGTT TGCTTTTAACATTAAAAGGGAAATGTGCCTCTGCCCCACAGTTAGAGGGGTGCACGTTCC TCCTGGTTCCTTCGCTTGTGTTTCTGTACTTACCAAAAATCTACCATTTCAATAAATTTT GATAGGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_177551 |
| Insert Size | 2200 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177551.3, NP_808219.1 |
| RefSeq Size | 2082 bp |
| RefSeq ORF | 1092 bp |
| Locus ID | 338442 |
| UniProt ID | Q8TDS4 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206527 | HCAR2 (Myc-DDK-tagged)-Human G protein-coupled receptor 109A (GPR109A) |
CNY 3,656.00 |
|
| RC206527L1 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206527L2 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), mGFP tagged |
CNY 6,056.00 |
|
| RC206527L3 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206527L4 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), mGFP tagged |
CNY 5,890.00 |
|
| RG206527 | HCAR2 (tGFP-tagged) - Human G protein-coupled receptor 109A (GPR109A) |
CNY 5,256.00 |
