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HCAR2 (NM_177551) Human Tagged ORF Clone
CAT＃: RC206527
HCAR2 (Myc-DDK-tagged)-Human G protein-coupled receptor 109A (GPR109A)
ORF Plasmid: tGFP
"NM_177551" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GPR109A; HCA2; HM74a; HM74b; NIACR1; Puma-g; PUMAG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206527 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCGGCACCATCTGCAGGATCACTTTCTGGAAATAGACAAGAAGAACTGCTGCGTGTTCCGAGATG ACTTCATTGTCAAGGTGTTGCCGCCGGTGTTGGGGCTGGAGTTTATCTTCGGGCTTCTGGGCAATGGCCT TGCCCTGTGGATTTTCTGTTTCCACCTCAAGTCCTGGAAATCCAGCCGGATTTTCCTGTTCAACCTGGCA GTGGCTGACTTTCTACTGATCATCTGCCTGCCCTTCCTGATGGACAACTATGTGAGGCGTTGGGACTGGA AGTTTGGGGACATCCCTTGCCGGCTGATGCTCTTCATGTTGGCTATGAACCGCCAGGGCAGCATCATCTT CCTCACGGTGGTGGCGGTAGACAGGTATTTCCGGGTGGTCCATCCCCACCACGCCCTGAACAAGATCTCC AATCGGACAGCAGCCATCATCTCTTGCCTTCTGTGGGGCATCACTATTGGCCTGACAGTCCACCTCCTGA AGAAGAAGATGCCGATCCAGAATGGCGGTGCAAATTTGTGCAGCAGCTTCAGCATCTGCCATACCTTCCA GTGGCACGAAGCCATGTTCCTCCTGGAGTTCTTCCTGCCCCTGGGCATCATCCTGTTCTGCTCAGCCAGA ATTATCTGGAGCCTGCGGCAGAGACAAATGGACCGGCATGCCAAGATCAAGAGAGCCATCACCTTCATCA TGGTGGTGGCCATCGTCTTTGTCATCTGCTTCCTTCCCAGCGTGGTTGTGCGGATCCGCATCTTCTGGCT CCTGCACACTTCGGGCACGCAGAATTGTGAAGTGTACCGCTCGGTGGACCTGGCGTTCTTTATCACTCTC AGCTTCACCTACATGAACAGCATGCTGGACCCCGTGGTGTACTACTTCTCCAGCCCATCCTTTCCCAACT TCTTCTCCACTTTGATCAACCGCTGCCTCCAGAGGAAGATGACAGGTGAGCCAGATAATAACCGCAGCAC GAGCGTCGAGCTCACAGGGGACCCCAACAAAACCAGAGGCGCTCCAGAGGCGTTAATGGCCAACTCCGGT GAGCCATGGAGCCCCTCTTATCTGGGCCCAACCTCTCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206527 protein sequence
Red=Cloning site Green=Tags(s) MNRHHLQDHFLEIDKKNCCVFRDDFIVKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLA VADFLLIICLPFLMDNYVRRWDWKFGDIPCRLMLFMLAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKIS NRTAAIISCLLWGITIGLTVHLLKKKMPIQNGGANLCSSFSICHTFQWHEAMFLLEFFLPLGIILFCSAR IIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIRIFWLLHTSGTQNCEVYRSVDLAFFITL SFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKMTGEPDNNRSTSVELTGDPNKTRGAPEALMANSG EPWSPSYLGPTSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177551 |
| ORF Size | 1089 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177551.4 |
| RefSeq Size | 2082 bp |
| RefSeq ORF | 1092 bp |
| Locus ID | 338442 |
| UniProt ID | Q8TDS4 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 41.8 kDa |
| Gene Summary | Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
5-Aminosalicylic Acid Azo-Coupled with a GPR109A Agonist Is a Colon-Targeted Anticolitic Codrug with a Reduced Risk of Skin Toxicity
,Jeong, S;Lee, H;Kim, S;Ju, S;Kim, W;Cho, H;Kim, HY;Heo, G;Im, E;Yoo, JW;Yoon, IS;Jung, Y;,
Mol. Pharm.
,PubMed ID 31743034
[HCAR2]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206527L1 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206527L2 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), mGFP tagged |
CNY 5,890.00 |
|
| RC206527L3 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206527L4 | Lenti ORF clone of Human G protein-coupled receptor 109A (GPR109A), mGFP tagged |
CNY 5,890.00 |
|
| RG206527 | HCAR2 (tGFP-tagged) - Human G protein-coupled receptor 109A (GPR109A) |
CNY 4,370.00 |
|
| SC309249 | HCAR2 (untagged)-Human G protein-coupled receptor 109A (GPR109A) |
CNY 3,656.00 |
