UBR2 (NM_015255) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309190 representing NM_015255. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGTCGGAGCTAGAGCCAGAGGTGCAGGCCATCGACCGGAGCTTGCTGGAATGTTCGGCCGAGGAG ATTGCGGGGAAATGGCTGCAAGCAACTGACCTCACTAGAGAAGTGTACCAGCATTTAGCCCACTATGTA CCCAAAATCTACTGCAGGGGTCCCAACCCTTTTCCACAGAAAGAAGACATGCTGGCACAGCATGTTTTG TTGGGACCAATGGAATGGTACCTTTGTGGTGAAGATCCTGCATTTGGATTTCCAAAACTTGAGCAAGCA AACAAACCTTCTCATCTTTGTGGTCGTGTTTTTAAAGTAGGAGAGCCTACATATTCTTGCAGAGACTGT GCAGTTGATCCAACTTGTGTTTTGTGCATGGAGTGCTTTTTGGGAAGTATTCACAGAGATCATCGATAT AGGATGACAACATCAGGAGGTGGAGGTTTCTGTGACTGTGGTGATACTGAAGCCTGGAAAGAGGGTCCT TACTGTCAAAAACATGAACTTAACACCTCTGAAATTGAGGAAGAAGAGGATCCTCTTGTTCATTTATCA GAAGATGTGATAGCAAGAACTTATAACATTTTTGCTATTACGTTTCGGTATGCAGTAGAAATATTAACC TGGGAAAAAGAAAGTGAATTGCCAGCAGATTTAGAGATGGTAGAGAAGAGTGACACCTACTATTGCATG CTGTTTAATGATGAGGTTCACACCTATGAACAAGTTATTTATACTCTTCAGAAAGCTGTTAACTGTACA CAAAAAGAAGCTATTGGTTTTGCAACTACAGTAGATCGAGATGGGCGTAGGTCTGTTCGATATGGAGAT TTTCAGTATTGTGAGCAAGCAAAATCAGTAATTGTGAGAAATACCAGTAGACAGACAAAGCCACTCAAA GTTCAAGTTATGCATTCGTCTATTGTCGCACATCAGAATTTTGGTTTGAAACTTTTGTCTTGGCTGGGA AGTATTATTGGATATTCAGATGGCCTTCGCCGGATTTTATGTCAAGTTGGTTTACAAGAAGGGCCAGAT GGTGAAAACTCTTCTCTAGTGGACAGACTGATGCTTAGTGATTCCAAATTATGGAAAGGTGCTAGGAGT GTATATCATCAGTTGTTCATGAGCAGTCTGCTTATGGATTTGAAATACAAGAAACTATTTGCTGTTCGA TTTGCAAAAAATTACCAGCAGTTGCAGAGAGATTTTATGGAGGATGATCACGAGCGAGCAGTGTCGGTG ACTGCTCTATCTGTCCAGTTCTTCACCGCACCTACTCTGGCTCGAATGCTCATCACAGAAGAAAACTTA ATGAGCATTATCATTAAGACTTTTATGGATCATTTGAGACATCGAGATGCCCAGGGCAGATTTCAGTTT GAACGATACACTGCTTTACAAGCCTTCAAATTTAGGAGAGTACAGAGCCTTATTTTAGATCTCAAGTAT GTGTTAATTAGCAAACCAACTGAATGGTCAGATGAGCTGAGGCAGAAGTTCCTAGAAGGGTTTGATGCC TTTTTGGAATTACTAAAATGTATGCAGGGAATGGATCCAATTACACGTCAAGTAGGACAACATATTGAA ATGGAACCAGAGTGGGAAGCAGCCTTCACACTACAAATGAAATTAACACATGTCATTTCAATGATGCAG GACTGGTGTGCTTCAGATGAAAAAGTGTTAATCGAAGCTTACAAGAAATGTCTCGCTGTACTGATGCAG TGTCATGGTGGTTATACTGATGGTGAACAGCCAATCACACTAAGCATTTGTGGACATTCAGTGGAAACT ATCAGATACTGTGTTTCCCAAGAAAAAGTTAGCATTCACCTCCCAGTTTCTCGCTTACTTGCAGGTTTA CATGTATTATTAAGCAAAAGTGAAGTGGCATATAAATTTCCAGAGCTCCTACCTCTAAGTGAACTTAGC CCACCCATGTTGATAGAACACCCTCTTAGATGTCTTGTTCTGTGTGCCCAAGTACATGCCGGAATGTGG AGAAGAAATGGGTTCTCTCTAGTAAACCAGATTTATTACTACCATAATGTGAAATGCAGACGTGAGATG TTTGACAAGGATGTAGTAATGCTTCAGACAGGTGTCTCCATGATGGATCCAAATCATTTCCTGATGATC ATGCTCAGCCGCTTTGAACTTTATCAGATTTTCAGTACTCCAGACTATGGAAAAAGATTTAGTTCTGAG ATTACCCATAAGGATGTTGTTCAGCAGAACAATACTCTAATAGAAGAAATGCTATACCTCATTATAATG CTTGTTGGAGAGAGATTTAGTCCTGGAGTTGGACAGGTAAATGCTACAGATGAAATCAAGCGAGAGATT ATCCATCAGTTGAGTATCAAGCCTATGGCTCATAGTGAATTGGTAAAGTCTTTACCTGAAGATGAGAAC AAGGAGACTGGCATGGAGAGTGTAATCGAAGCAGTTGCCCATTTCAAGAAACCTGGATTAACAGGACGA GGCATGTATGAACTGAAACCAGAATGTGCCAAAGAGTTCAACTTGTATTTCTATCACTTTTCAAGGGCA GAACAGTCCAAGGCAGAAGAAGCGCAACGGAAATTGAAAAGACAAAATAGAGAAGATACAGCACTCCCA CCTCCGGTGTTGCCTCCATTCTGCCCTCTGTTTGCAAGCCTGGTTAACATTTTGCAGTCAGATGTCATG TTGTGCATCATGGGAACAATTCTGCAATGGGCTGTGGAACATAATGGATATGCCTGGTCAGAGTCCATG CTGCAAAGGGTGTTACATTTAATTGGCATGGCACTACAAGAAGAAAAACAACATTTAGAGAATGTCACG GAAGAGCATGTAGTAACATTTACCTTCACTCAGAAGATATCAAAACCTGGTGAAGCGCCAAAAAATTCT CCTAGCATACTAGCTATGCTGGAAACACTACAAAATGCTCCCTACCTAGAAGTCCACAAAGACATGATT CGGTGGATATTGAAGACTTTTAATGCTGTTAAAAAGATGAGGGAGAGTTCACCTACCAGTCCCGTGGCA GAGACAGAAGGAACCATAATGGAAGAGAGTTCAAGGGACAAAGACAAAGCTGAGAGGAAGAGAAAAGCA GAGATTGCCAGACTGCGCAGAGAAAAGATCATGGCTCAGATGTCTGAAATGCAGCGGCATTTTATTGAT GAAAACAAAGAACTCTTTCAGCAGACATTAGAACTGGATGCCTCAACCTCTGCTGTTCTTGATCATAGC CCTGTGGCTTCAGATATGACACTTACAGCACTGGGCCCCGCACAAACTCAGGTTCCTGAACAAAGACAA TTCGTTACATGTATATTGTGTCAAGAGGAGCAAGAAGTTAAAGTGGAAAGCAGGGCAATGGTCTTGGCA GCATTTGTTCAGAGATCAACTGTATTATCAAAAAACAGAAGTAAATTTATTCAAGATCCAGAAAAATAT GATCCATTATTCATGCACCCTGATCTGTCTTGTGGAACACACACTAGTAGCTGTGGGCACATTATGCAT GCCCATTGTTGGCAAAGGTATTTTGATTCCGTTCAAGCTAAAGAACAGCGAAGGCAACAGAGATTACGC TTACATACGAGCTATGATGTAGAAAACGGAGAATTCCTTTGCCCCCTTTGTGAATGCTTGAGTAATACT GTTATTCCTCTGCTGCTTCCTCCAAGAAATATTTTTAACAACAGGTTAAATTTTTCAGACCAACCAAAT CTGACTCAGTGGATTAGAACAATATCTCAGCAAATAAAAGCATTACAGTTTCTTAGGAAAGAAGAAAGT ACTCCTAATAATGCCTCTACAAAGAATTCAGAAAATGTGGATGAATTACAGCTCCCTGAAGGGTTCAGG CCTGATTTTCGTCCTAAGATCCCTTATTCTGAGAGCATAAAAGAAATGCTAACGACATTTGGAACTGCT ACCTACAAGGTGGGACTAAAGGTTCATCCCAATGAAGAGGATCCTCGTGTTCCCATAATGTGTTGGGGT AGCTGCGCGTACACCATCCAAAGCATAGAAAGAATTTTGAGTGATGAAGATAAACCATTGTTTGGTCCT TTACCTTGCAGACTGGATGACTGTCTTAGGTCATTGACGAGATTTGCCGCAGCACACTGGACAGTGGCA TCAGTTTCAGTGGTGCAAGGACATTTTTGTAAACTTTTTGCATCACTGGTGCCTAATGACAGCCATGAG GAACTTCCATGCATATTAGATATTGACATGTTTCATTTATTGGTGGGCTTGGTGCTTGCATTTCCTGCG TTGCAGTGTCAGGATTTTTCAGGGATCAGCCTTGGCACTGGAGACCTTCACATTTTCCATCTGGTTACT ATGGCACACATCATACAGATCTTACTTACCTCATGTACAGAAGAGAATGGCATGGATCAAGAAAATCCC CCTTGTGAAGAAGAATCAGCAGTTCTTGCTTTGTATAAAACACTTCACCAGTATACGGGAAGTGCCTTG AAAGAAATACCATCCGGCTGGCATCTGTGGAGGAGTGTCAGAGCTGGAATCATGCCTTTCCTGAAGTGT TCTGCTTTATTTTTTCATTACTTAAATGGAGTTCCTTCCCCACCCGACATTCAAGTTCCTGGAACAAGC CATTTTGAACATTTATGTAGCTATCTTTCCCTACCAAACAACCTCATTTGCCTTTTTCAAGAAAATAGT GAGATAATGAATTCACTGATTGAAAGTTGGTGCCGTAACAGTGAAGTTAAAAGATATCTAGAAGGTGAA AGAGATGCTATAAGATATCCAAGAGAATCTAACAAATTAATAAACCTTCCAGAGGATTACAGCAGCCTC ATTAATCAAGCATCCAATTTCTCGTGCCCGAAATCAGGTGGTGATAAGAGCAGAGCCCCAACTCTGTGC CTTGTGTGCGGATCTCTGCTGTGCTCCCAGAGTTACTGCTGCCAGACTGAACTGGAAGGGGAGGATGTA GGAGCCTGCACAGCTCACACCTACTCCTGTGGCTCTGGAGTGGGCATCTTCCTGAGAGTACGGGAATGT CAGGTGCTATTTTTAGCTGGCAAAACCAAAGGCTGTTTTTATTCTCCTCCTTACCTTGATGACTATGGG GAGACCGACCAGGGACTCAGACGGGGAAATCCTTTACATTTATGCAAAGAGCGATTCAAGAAGATTCAG AAGCTCTGGCACCAACACAGTGTCACAGAGGAAATTGGACATGCACAGGAAGCCAATCAGACACTGGTT GGCATTGACTGGCAACATTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_015255
Insert Size	5268 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_015255.2
RefSeq Size	7898 bp
RefSeq ORF	5268 bp
Locus ID	23304
UniProt ID	Q8IWV8
Protein Families	Druggable Genome
MW	200.5 kDa
Gene Summary	This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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