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UBR2 (NM_015255) Human Tagged ORF Clone
CAT＃: RG224120
- TrueORF®
UBR2 (tGFP-tagged) - Human ubiquitin protein ligase E3 component n-recognin 2 (UBR2), transcript variant 1
ORF Plasmid: DDK
"NM_015255" in other vectors (2)
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CNY 19,190.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG224120 representing NM_015255
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGAGCTAGAGCCAGAGGTGCAGGCCATCGACCGGAGTTTGCTGGAATGTTCGGCCGAGGAGA TTGCGGGGAAATGGCTGCAAGCAACTGACCTCACTAGAGAAGTGTACCAGCATTTAGCCCACTATGTACC CAAAATCTACTGCAGGGGTCCCAACCCTTTTCCACAGAAAGAAGACATGCTGGCACAGCATGTTTTGTTG GGACCAATGGAATGGTACCTTTGTGGTGAAGATCCTGCATTTGGATTTCCAAAACTTGAGCAAGCAAACA AACCTTCTCATCTTTGTGGTCGTGTTTTTAAAGTAGGAGAGCCTACATATTCTTGCAGAGACTGTGCAGT TGATCCAACTTGTGTTTTGTGCATGGAGTGCTTTTTGGGAAGTATTCACAGAGATCATCGATATAGGATG ACAACATCAGGAGGTGGAGGTTTCTGTGACTGTGGTGATACTGAAGCCTGGAAAGAGGGTCCTTACTGTC AAAAACATGAACTTAACACCTCTGAAATTGAGGAAGAAGAGGATCCTCTTGTTCATTTATCAGAAGATGT GATAGCAAGAACTTATAACATTTTTGCTATTACGTTTCGGTATGCAGTAGAAATATTAACCTGGGAAAAA GAAAGTGAATTGCCAGCAGATTTAGAGATGGTAGAGAAGAGTGACACCTACTATTGCATGCTGTTTAATG ATGAGGTTCACACCTATGAACAAGTTATTTATACTCTTCAGAAAGCTGTTAACTGTACACAAAAAGAAGC TATTGGTTTTGCAACTACAGTAGATCGAGATGGGCGTAGGTCTGTTCGATATGGAGATTTTCAGTATTGT GAGCAAGCAAAATCAGTAATTGTGAGAAATACCAGTAGACAGACAAAGCCACTCAAAGTTCAAGTTATGC ATTCGTCTATTGTCGCACATCAGAATTTTGGTTTGAAACTTTTGTCTTGGCTGGGAAGTATTATTGGATA TTCAGATGGCCTTCGCCGGATTTTATGTCAAGTTGGTTTACAAGAAGGGCCAGATGGTGAAAACTCTTCT CTAGTGGACAGACTGATGCTTAGTGATTCCAAATTATGGAAAGGTGCTAGGAGTGTATATCATCAGTTGT TCATGAGCAGTCTGCTTATGGATTTGAAATACAAGAAACTATTTGCTGTTCGATTTGCAAAAAATTACCA GCAGTTGCAGAGAGATTTTATGGAGGATGATCACGAGCGAGCAGTGTCGGTGACTGCTCTATCTGTCCAG TTCTTCACCGCACCTACTCTGGCTCGAATGCTCATCACAGAAGAAAACTTGATGAGCATTATCATTAAGA CTTTTATGGATCATTTGAGACATCGAGATGCCCAGGGCAGATTTCAGTTTGAACGATACACTGCTTTACA AGCCTTCAAATTTAGGAGAGTACAGAGCCTTATTTTAGATCTCAAGTATGTGTTAATTAGCAAACCAACT GAATGGTCAGATGAGCTGAGGCAGAAGTTCCTAGAAGGGTTTGATGCCTTTTTGGAATTACTAAAATGTA TGCAGGGAATGGATCCAATTACACGTCAAGTAGGACAACATATTGAAATGGAACCAGAGTGGGAAGCAGC CTTCACACTACAAATGAAATTAACACATGTCATTTCAATGATGCAGGACTGGTGTGCTTCAGATGAAAAA GTGTTAATCGAAGCTTACAAGAAATGTCTCGCTGTACTGATGCAGTGTCATGGTGGTTATACTGATGGTG AACAGCCAATCACACTAAGCATTTGTGGACATTCAGTGGAAACTATCAGATACTGTGTTTCCCAAGAAAA AGTTAGCATTCACCTCCCAGTTTCTCGCTTACTTGCAGGTTTACATGTATTATTAAGCAAAAGTGAAGTG GCATATAAATTTCCAGAGCTCCTACCTCTAAGTGAACTTAGCCCACCCATGTTGATAGAACACCCTCTTA GATGTCTTGTTCTGTGTGCCCAAGTACATGCCGGAATGTGGAGAAGAAATGGGTTCTCTCTAGTAAACCA GATTTATTACTACCATAATGTGAAATGCAGACGTGAGATGTTTGACAAGGATGTAGTAATGCTTCAGACA GGTGTCTCCATGATGGATCCAAATCATTTCCTGATGATCATGCTCAGCCGCTTTGAACTTTATCAGATTT TCAGTACTCCAGACTATGGAAAAAGATTTAGTTCTGAGATTACCCATAAGGATGTTGTTCAGCAGAACAA TACTCTAATAGAAGAAATGCTATACCTCATTATAATGCTTGTTGGAGAGAGATTTAGTCCTGGAGTTGGA CAGGTAAATGCTACAGATGAAATCAAGCGAGAGATTATCCATCAGTTGAGTATCAAGCCTATGGCTCATA GTGAATTGGTAAAGTCTTTACCTGAAGATGAGAACAAGGAGACTGGCATGGAGAGTGTAATCGAAGCAGT TGCCCATTTCAAGAAACCTGGATTAACAGGACGAGGCATGTATGAACTGAAACCAGAATGTGCCAAAGAG TTCAACTTGTATTTCTATCACTTTTCAAGGGCAGAACAGTCCAAGGCAGAAGAAGCGCAACGGAAATTGA AAAGACAAAATAGAGAAGATACAGCACTCCCACCTCCGGTGTTGCCTCCATTCTGCCCTCTGTTTGCAAG CCTGGTTAACATTTTGCAGTCAGATGTCATGTTGTGCATCATGGGAACAATTCTGCAATGGGCTGTGGAA CATAATGGATATGCCTGGTCAGAGTCCATGCTGCAAAGGGTGTTACATTTAATTGGCATGGCACTACAAG AAGAAAAACAACATTTAGAGAATGTCACGGAAGAGCATGTAGTAACATTTACCTTCACTCAGAAGATATC AAAACCTGGTGAAGCGCCAAAAAATTCTCCTAGCATACTAGCTATGCTGGAAACACTACAAAATGCTCCC TACCTAGAAGTCCACAAAGACATGATTCGGTGGATATTGAAGACTTTTAATGCTGTTAAAAAGATGAGGG AGAGTTCACCTACCAGTCCCGTGGCAGAGACAGAAGGAACCATAATGGAAGAGAGTTCAAGGGACAAAGA CAAAGCTGAGAGGAAGAGAAAAGCAGAGATTGCCAGACTGCGCAGAGAAAAGATCATGGCTCAGATGTCT GAAATGCAGCGGCATTTTATTGATGAAAACAAAGAACTCTTTCAGCAGACATTAGAACTGGATGCCTCAA CCTCTGCTGTTCTTGATCATAGCCCTGTGGCTTCAGATATGACACTTACAGCACTGGGCCCCGCACAAAC TCAGGTTCCTGAACAAAGACAATTCGTTACATGTATATTGTGTCAAGAGGAGCAAGAAGTTAAAGTGGAA AGCAGGGCAATGGTCTTGGCAGCATTTGTTCAGAGATCAACTGTATTATCAAAAAACAGAAGTAAATTTA TTCAAGATCCAGAAAAATATGATCCATTATTCATGCACCCTGATCTGTCTTGTGGAACACACACTAGTAG CTGTGGGCACATTATGCATGCCCATTGTTGGCAAAGGTATTTTGATTCCGTTCAAGCTAAAGAACAGCGA AGGCAACAGAGATTACGCTTACATACGAGCTATGATGTAGAAAACGGAGAATTCCTTTGCCCCCTTTGTG AATGCTTGAGTAATACTGTTATTCCTCTGCTGCTTCCTCCAAGAAATATTTTTAACAACAGGTTAAATTT TTCAGACCAACCAAATCTGACTCAGTGGATTAGAACAATATCTCAGCAAATAAAAGCATTACAGTTTCTT AGGAAAGAAGAAAGTACTCCTAATAATGCCTCTACAAAGAATTCAGAAAATGTGGATGAATTACAGCTCC CTGAAGGGTTCAGGCCTGATTTTCGTCCTAAGATCCCTTATTCTGAGAGCATAAAAGAAATGCTAACGAC ATTTGGAACTGCTACCTACAAGGTGGGACTAAAGGTTCATCCCAATGAAGAGGATCCTCGTGTTCCCATA ATGTGTTGGGGTAGCTGCGCGTACACCATCCAAAGCATAGAAAGAATTTTGAGTGATGAAGATAAACCAT TGTTTGGTCCTTTACCTTGCAGACTGGATGACTGTCTTAGGTCATTGACGAGATTTGCCGCAGCACACTG GACAGTGGCATCAGTTTCAGTGGTGCAAGGACATTTTTGTAAACTTTTTGCATCACTGGTGCCTAATGAC AGCCATGAGGAACTTCCATGCATATTAGATATTGACATGTTTCATTTATTGGTGGGCTTGGTGCTTGCAT TTCCTGCGTTGCAGTGTCAGGATTTTTCAGGGATCAGCCTTGGCACTGGAGACCTTCACATTTTCCATCT GGTTACTATGGCACACATCATACAGATCTTACTTACCTCATGTACAGAAGAGAATGGCATGGATCAAGAA AATCCCCCTTGTGAAGAAGAATCAGCAGTTCTTGCTTTGTATAAAACACTTCACCAGTATACGGGAAGTG CCTTGAAAGAAATACCATCCGGCTGGCATCTGTGGAGGAGTGTCAGAGCTGGAATCATGCCTTTCCTGAA GTGTTCTGCTTTATTTTTTCATTACTTAAATGGAGTTCCTTCCCCACCCGACATTCAAGTTCCTGGAACA AGCCATTTTGAACATTTATGTAGCTATCTTTCCCTACCAAACAACCTCATTTGCCTTTTTCAAGAAAATA GTGAGATAATGAATTCACTGATTGAAAGTTGGTGCCGTAACAGTGAAGTTAAAAGATATCTAGAAGGTGA AAGAGATGCTATAAGATATCCAAGAGAATCTAACAAATTAATAAACCTTCCAGAGGATTACAGCAGCCTC ATTAATCAAGCATCCAATTTCTCGTGCCCGAAATCAGGTGGTGATAAGAGCAGAGCCCCAACTCTGTGCC TTGTGTGCGGATCTCTGCTGTGCTCCCAGAGTTACTGCTGCCAGACTGAACTGGAAGGGGAGGATGTAGG AGCCTGCACAGCTCACACCTACTCCTGTGGCTCTGGAGTGGGCATCTTCCTGAGAGTACGGGAATGTCAG GTGCTATTTTTAGCTGGCAAAACCAAAGGCTGTTTTTATTCTCCTCCTTACCTTGATGACTATGGGGAGA CCGACCAGGGACTCAGACGGGGAAATCCTTTACATTTATGCAAAGAGCGATTCAAGAAGATTCAGAAGCT CTGGCACCAACACAGTGTCACAGAGGAAATTGGACATGCACAGGAAGCCAATCAGACACTGGTTGGCATT GACTGGCAACATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG224120 representing NM_015255
Red=Cloning site Green=Tags(s) MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLL GPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRM TTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAITFRYAVEILTWEK ESELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYC EQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSS LVDRLMLSDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQ FFTAPTLARMLITEENLMSIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPT EWSDELRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCASDEK VLIEAYKKCLAVLMQCHGGYTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEV AYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDVVMLQT GVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVG QVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKE FNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMGTILQWAVE HNGYAWSESMLQRVLHLIGMALQEEKQHLENVTEEHVVTFTFTQKISKPGEAPKNSPSILAMLETLQNAP YLEVHKDMIRWILKTFNAVKKMRESSPTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMS EMQRHFIDENKELFQQTLELDASTSAVLDHSPVASDMTLTALGPAQTQVPEQRQFVTCILCQEEQEVKVE SRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQR RQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNNRLNFSDQPNLTQWIRTISQQIKALQFL RKEESTPNNASTKNSENVDELQLPEGFRPDFRPKIPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPI MCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASVSVVQGHFCKLFASLVPND SHEELPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIIQILLTSCTEENGMDQE NPPCEEESAVLALYKTLHQYTGSALKEIPSGWHLWRSVRAGIMPFLKCSALFFHYLNGVPSPPDIQVPGT SHFEHLCSYLSLPNNLICLFQENSEIMNSLIESWCRNSEVKRYLEGERDAIRYPRESNKLINLPEDYSSL INQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQ VLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSVTEEIGHAQEANQTLVGI DWQHL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015255 |
| ORF Size | 5265 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015255.1, NP_056070.1 |
| RefSeq Size | 5268 bp |
| RefSeq ORF | 5268 bp |
| Locus ID | 23304 |
| UniProt ID | Q8IWV8 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes an E3 ubiquitin ligase of the N-end rule proteolytic pathway that targets proteins with destabilizing N-terminal residues for polyubiquitylation and proteasome-mediated degradation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
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