NUP98 (NM_016320) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADIR2; NUP96; Nup98-96; NUP196
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309117 representing NM_016320. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTAACAAATCATTTGGAACACCCTTTGGGGGTGGCACAGGTGGCTTTGGCACAACTTCAACATTT GGACAGAATACTGGCTTTGGCACTACTAGTGGAGGGGCATTTGGAACATCTGCATTTGGTTCTAGCAAC AATACTGGAGGCCTCTTTGGAAATTCACAGACTAAACCAGGAGGATTGTTTGGAACCAGTTCATTTAGC CAGCCAGCTACCTCCACAAGCACTGGCTTTGGGTTTGGTACGTCAACAGGAACAGCAAATACCTTGTTT GGAACTGCAAGCACAGGGACCAGTCTCTTCTCATCCCAAAACAATGCCTTTGCACAAAATAAACCAACT GGCTTTGGCAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACCACAAATACCACCTCTAAT CCTTTTGGCAGCACATCTGGCTCCCTCTTTGGGCCAAGTAGTTTTACAGCTGCTCCTACTGGGACTACT ATTAAATTTAACCCTCCAACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATAAGTACC AAGCACCAGTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCACTAGAGGAACTTCGTTTAGAGGAT TATCAGGCTAACAGGAAGGGCCCACAGAACCAGGTGGGAGCAGGTACCACAACTGGCTTGTTTGGGTCT TCTCCAGCCACTTCCAGCGCAACAGGACTCTTCAGCTCCTCCACCACTAATTCAGGCTTTGCATATGGT CAGAACAAAACTGCCTTTGGAACTAGTACAACTGGATTTGGAACAAATCCAGGTGGTCTCTTTGGCCAA CAGAATCAGCAGACTACCAGCCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCAGAACACTGGC TTTTCCTTTGGTAATACCAGCACCATAGGACAGCCAAGCACCAACACCATGGGATTATTTGGAGTAACC CAAGCCTCACAGCCTGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGGACAGCATTTGGAACA GGAACAGGTCTCTTTGGGCAGACCAATACTGGATTTGGTGCTGTTGGTTCGACCCTGTTTGGCAATAAC AAGCTTACTACATTTGGAAGCAGCACAACCAGTGCACCTTCATTTGGTACAACCAGTGGCGGGCTCTTT GGTTTTGGCACAAATACCAGTGGGAATAGTATTTTTGGAAGTAAACCAGCACCTGGGACTCTTGGAACT GGGCTTGGTGCAGGATTTGGAACAGCTCTTGGTGCTGGACAGGCATCTTTGTTTGGGAACAACCAACCT AAGATTGGAGGGCCTCTTGGTACAGGAGCCTTTGGGGCCCCTGGATTTAATACTACGACAGCCACTTTG GGCTTTGGAGCCCCCCAGGCCCCAGTAGCTTTGACAGATCCAAATGCTTCTGCTGCCCAGCAGGCTGTT CTCCAGCAGCACATCAATAGTCTAACATACTCACCTTTTGGAGACTCTCCTCTCTTCCGGAATCCGATG TCAGACCCTAAGAAGAAGGAAGAGAGATTGAAACCAACAAATCCAGCAGCCCAGAAGGCTCTTACTACA CCTACTCATTATAAACTGACACCCCGCCCTGCCACTAGAGTCCGGCCAAAGGCTTTACAAACAACAGGC ACAGCCAAGTCACATCTCTTTGATGGGCTGGATGACGATGAACCATCCCTAGCCAATGGAGCATTCATG CCCAAGAAGAGCATTAAGAAGTTGGTTTTGAAGAACCTTAATAATAGCAATCTCTTTTCTCCTGTTAAT CGTGATTCAGAAAATCTAGCTTCACCATCTGAATATCCAGAAAATGGAGAGAGATTTAGTTTCCTAAGC AAACCTGTTGATGAGAATCACCAGCAGGATGGAGATGAAGATTCCCTTGTTTCACATTTTTATACTAAC CCTATTGCCAAACCTATTCCTCAAACCCCAGAAAGTGCTGGAAATAAACACAGCAACAGCAACAGTGTG GATGATACCATTGTTGCATTAAACATGCGTGCTGCTTTGCGAAATGGGCTGGAAGGAAGCAGTGAAGAA ACGTCTTTTCATGATGAGTCACTTCAGGATGACCGAGAAGAAATAGAAAATAATTCTTACCATATGCAC CCAGCAGGTATTATTCTCACTAAGGTTGGTTACTATACTATTCCATCTATGGATGACCTTGCTAAAATT ACCAATGAAAAAGGAGAGTGCATTGTCTCTGATTTCACTATTGGTCGGAAAGGTTATGGTTCAATCTAT TTTGAAGGAGATGTGAATTTGACAAATCTAAATTTGGATGATATTGTGCATATCCGGAGGAAAGAAGTA GTTGTCTACTTAGATGATAACCAAAAACCACCTGTGGGTGAAGGGCTAAATAGGAAGGCTGAAGTTACA TTGGATGGAGTTTGGCCAACAGATAAAACATCTCGTTGTTTAATAAAGAGCCCAGATCGCCTTGCTGAT ATCAACTATGAAGGAAGATTGGAAGCAGTTTCAAGGAAACAGGGAGCTCAATTCAAAGAATACCGGCCT GAAACTGGTTCTTGGGTGTTTAAGGTCTCCCATTTTTCTAAGTATGGCCTTCAGGATTCTGATGAAGAG GAGGAGGAGCATCCGTCTAAAACTAGTACAAAGAAGTTGAAGACTGCTCCTTTGCCTCCTGCAAGCCAG ACTACGCCCTTGCAGATGGCTCTTAATGGCAAACCTGCACCTCCACCTCAGAGCCAGAGCCCAGAGGTG GAGCAGTTAGGGAGGGTTGTGGAACTGGACAGTGACATGGTAGATATCACCCAGGAGCCAGTTTTGGAT ACCATGTTAGAAGAGAGCATGCCTGAGGATCAGGAACCTGTGTCTGCCTCAACACATATTGCATCTTCA CTGGGAATTAATCCACATGTCTTACAGATCATGAAAGCATCATTGCTTACTGATGAAGAAGATGTAGAT ATGGCACTGGATCAACGCTTCAGTCGCCTGCCTTCCAAAGCAGATACTTCTCAAGAAATCTGTTCTCCC AGACTCCCCATTTCAGCATCCCACTCGTCGAAAACTCGTTCACTAGTTGGTGGGTTACTACAATCAAAA TTTACAAGTGGAGCTTTTCTTTCACCAAGTGTCTCTGTGCAAGAATGTCGTACTCCCAGAGCAGCATCT TTAATGAATATCCCATCCACATCCTCTTGGTCTGTCCCTCCACCCCTGACTTCTGTGTTCACAATGCCC AGCCCAGCCCCTGAGGTTCCGTTGAAAACAGTGGGTACACGTAGGCAACTAGGCCTAGTCCCTCGTGAA AAGTCTGTCACCTATGGCAAGGGAAAACTCTTGATGGACATGGCCCTATTCATGGGACGCTCATTTCGT GTTGGTTGGGGCCCCAACTGGACTCTTGCTAATAGTGGAGAACAGCTGAATGGCTCTCATGAACTAGAA AATCATCAGATTGCCGATTCCATGGAGTTTGGATTCCTGCCCAATCCAGTAGCTGTTAAACCCCTAACT GAGTCCCCATTCAAAGTTCACTTAGAAAAACTCAGTTTGAGACAAAGAAAGCCAGATGAAGACATGAAA TTATATCAGACACCTCTGGAGCTCAAATTAAAACACAGCACTGTCCATGTGGATGAACTGTGTCCTCTC ATTGTCCCCAATCTGGGAGTTGCTGTCATTCATGACTATGCAGATTGGGTTAAAGAAGCATCAGGAGAC TTACCAGAAGCACAAATTGTGAAGCACTGGAGCCTGACATGGACACTATGTGAAGCCCTATGGGGCCAC CTGAAGGAGCTTGACAGCCAGCTAAATGAACCCCGTGAATACATTCAAATTCTGGAGCGAAGAAGAGCT TTCTCCCGCTGGCTATCCTGTACTGCCACACCTCAGATTGAAGAGGAAGTCTCCTTAACCCAAAAAAAC AGCCCTGTGGAGGCTGTATTCAGCTACCTCACAGGCAAAAGGATCAGTGAGGCCTGCTCTCTGGCCCAG CAGTCAGGGGATCATCGTCTTGCTCTTCTTTTATCTCAGTTTGTGGGTAGCCAGTCAGTCCGGGAGCTG CTCACCATGCAGTTGGTGGACTGGCATCAGCTCCAAGCAGACTCCTTCATCCAGGATGAGAGACTGCGC ATCTTTGCTCTGTTGGCTGGAAAACCGGTGTGGCAGCTCTCAGAAAAGAAGCAAATAAACGTGTGCTCC CAGTTGGATTGGAAACGCTCCCTGGCTATCCATCTTTGGTATTTGCTTCCACCAACAGCCTCCATTTCT AGGGCGCTCAGCATGTATGAAGAAGCATTTCAGAATACCTCTGACAGTGACAGATATGCCTGCTCCCCA CTTCCTTCGTATCTGGAGGGTTCTGGCTGTGTGATAGCGGAGGAGCAAAACTCACAGACACCACTTCGA GATGTCTGCTTTCACCTTCTAAAACTCTACAGTGACAGACATTATGATCTCAACCAGCTGCTGGAGCCT CGAAGCATAACAGCAGATCCTTTGGACTACCGCCTAAGCTGGCACTTGTGGGAAGTGCTGAGGGCTCTT AACTACACCCATCTCTCAGCGCAGTGTGAAGGTGTGCTACAGGCCAGTTACGCTGGCCAGCTTGAAAGT GAGGGGCTCTGGGAGTGGGCCATCTTTGTCCTCCTGCACATTGACAACTCAGGCATACGTGAGAAGGCT GTTCGAGAGCTGCTTACCCGGCACTGCCAGCTGTTGGAGACCCCTGAATCTTGGGCTAAAGAGACTTTC CTTACCCAGAAGCTCCGTGTACCTGCCAAATGGATCCACGAGGCCAAAGCTGTGCGAGCACACATGGAA TCTGACAAGCACTTAGAGGCCCTTTGCTTATTTAAGGCTGAGCACTGGAACCGCTGCCACAAGCTCATC ATCCGACACTTAGCTTCTGATGCCATCATTAATGAGAACTATGACTACCTGAAGGGGTTCTTGGAAGAC CTGGCACCTCCAGAGCGCAGCAGCCTAATTCAGGATTGGGAAACATCTGGGCTTGTTTACCTGGACTAT ATTAGAGTCATTGAAATGCTCCGCCATATACAGCAGGTGGATTGCTCAGGTAATGACCTGGAGCAGTTA CACATCAAAGTGACTTCACTGTGCAGTCGGATAGAGCAGATTCAGTGTTACAGTGCTAAAGATCGCCTG GCTCAGTCAGACATGGCCAAACGTGTAGCCAACCTGCTGCGCGTGGTGCTGAGTCTGCATCATCCTCCT GATAGAACCTCCGACTCAACACCAGACCCTCAGCGAGTCCCTTTGCGCCTCTTGGCTCCCCACATTGGC CGGCTTCCCATGCCTGAGGACTATGCCATGGACGAACTGCGCAGCCTTACCCAGTCCTATCTGCGAGAA CTGGCTGTTGGGAGCCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_016320
Insert Size	5403 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016320.4
RefSeq Size	6980 bp
RefSeq ORF	5403 bp
Locus ID	4928
UniProt ID	P52948
Domains	Nucleoporin_FG, Nucleoporin2
Protein Families	Druggable Genome
MW	195.8 kDa
Gene Summary	Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016] Transcript Variant: This variant (1) encodes isoform 1.

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