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SGPL1 (NM_003901) Human Untagged Clone
CAT＃: SC308998
SGPL1 (untagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1)
CNY 6,352.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NPHS14; S1PL; SPL |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_003901 edited
ATGCCTAGCACAGACCTTCTGATGTTGAAGGCCTTTGAGCCCTACTTAGAGATTTTGGAA GTATACTCCACAAAAGCCAAGAATTATGTAAATGGACATTGCACCAAGTATGAGCCCTGG CAGCTAATTGCATGGAGTGTCGTGTGGACCCTGCTGATAGTCTGGGGATATGAGTTTGTC TTCCAGCCAGAGAGTTTATGGTCAAGGTTTAAAAAGAAATGTTTTAAGCTCACCAGGAAG ATGCCCATTATTGGTCGTAAGATTCAAGACAAGTTGAACAAGACCAAGGATGATATTAGC AAGAACATGTCATTCCTGAAAGTGGACAAAGAGTATGTGAAAGCTTTACCCTCCCAGGGT CTGAGCTCATCTGCTGTTTTGGAGAAACTTAAGGAGTACAGCTCTATGGACGCCTTCTGG CAAGAGGGGAGAGCCTCTGGAACAGTGTACAGTGGGGAGGAGAAGCTCACTGAGCTCCTT GTGAAGGCTTATGGAGATTTTGCATGGAGTAACCCCCTGCATCCAGATATCTTCCCAGGA CTACGCAAGATAGAGGCAGAAATTGTGAGGATAGCTTGTTCCCTGTTCAATGGGGGACCA GATTCGTGTGGATGTGTGACTTCTGGGGGAACAGAAAGCATACTGATGGCCTGCAAAGCA TATCGGGATCTGGCCTTTGAGAAGGGGATCAAAACTCCAGAAATTGTGGCTCCCCAAAGT GCCCATGCTGCATTTAACAAAGCAGCCAGTTACTTTGGGATGAAGATTGTGCGGGTCCCA TTGACGAAGATGATGGAGGTGGATGTGCGGGCAATGAGAAGAGCTATCTCCAGGAACACT GCCATGCTCGTCTGTTCTACCCCACAGTTTCCTCATGGTGTAATAGATCCTGTCCCTGAA GTGGCCAAGCTGGCTGTCAAATACAAAATACCCCTTCATGTCGACGCTTGTCTGGGAGGC TTCCTCATCGTCTTTATGGAGAAAGCAGGATACCCACTGGAGCACCCATTTGATTTCCGG GTGAAAGGTGTAACCAGCATTTCAGCTGACACCCATAAGTATGGCTATGCCCCAAAAGGC TCATCATTGGTGTTGTATAGTGACAAGAAGTACAGGAACTATCAGTTCTTCGTCGATACA GATTGGCAGGGTGGCATCTATGCTTCCCCAACCATCGCAGGCTCACGGCCTGGTGGCATT AGCGCAGCCTGTTGGGCTGCCTTGATGCACTTCGGTGAGAACGGCTATGTTGAAGCTACC AAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTGGAAAATATCAAAGGCATC TTTGTTTTTGGGAATCCCCAATTGTCAGTCATTGCTCTGGGATCCCGTGATTTTGACATC TACCGACTATCAAACCTGATGACTGCTAAGGGGTGGAACTTGAACCAGTTGCAGTTCCCA CCCAGTATTCATTTCTGCATCACATTACTACACGCCCGGAAACGAGTAGCTATACAATTC CTAAAGGACATTCGAGAATCTGTCACTCAAATCATGAAGAATCCTAAAGCGAAGACCACA GGAATGGGTGCCATCTATGGCATGGCCCAGACAACTGTTGACAGGAATATGGTTGCAGAA TTGTCCTCAGTCTTCTTGGACAGCTTGTACAGCACCGACACTGTCACCCAGGGCAGCCAG ATGAATGGTTCTCCAAAACCCCACTGA >OriGene 5' read for NM_003901 unedited
GTTCNCATTTGTATACGACTCATATAGGCGGCCGCGNAATTCNATCTGGTACCGAGCTCG GATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGGCGTGAGGAGAGTCTGA AAAAGGGAGCGCGGAGAGGAGGCTGGAAGAGGAAGATGCCTAGCACAGACCTTCTGATGT TGAAGGCCTTTGAGCCCTACTTAGAGATTTTGGAAGTATACTCCACAAAAGCCAAGAATT ATGTAAATGGACATTGCACCAAGTATGAGCCCTGGCAGCTAATTGCATGGAGTGTCGTGT GGACCCTGCTGATAGTCTGGGGATATGAGTTTGTCTTCCAGCCAGAGAGTTTATGGTCAA GGTTTAAAAAGAAATGTTTTAAGCTCACCAGGAAGATGCCCATTATTGGTCGTAAGATTC AAGACAAGTTGAACAAGACCAAGGATGATATTAGCAAGAACATGTCATTCCTGAAAGTGG ACAAAGAGTATGTGAAAGCTTTACCCTCCCAGGGTCTGAGCTCATCTGCTGTTTTGGAGA AACTTAAGGAGTACAGCTCTATGGACGCCTTCTGGCAAGAGGGGAGAGCCTCTGGAACAG TGTACAGTGGGGAGGAGAAGCTCACTGAGCTCCTTGTGAAGGCTTATGGAGATTTTGCAT GGAGTAACCCCCTGCATCCAGATATCTTCCCAGGACTACGCAAGATAGAGGCAGAAATTG TGAGGATAGCTTGTTCCCTGTTCAAGGGGGACCAGATTCGTGTGGATGTGTGACTTCTGG GGGAACAGAAAGCATACTGATGGCCTGCANAGCATATCGGGATC >OriGene 3' genomic read for NM_003901 unedited
NCTGTTAATGGGGGNACCACTATCGTGTGGNTGTGTGACTTCTGGGGGAACANAAGCATA CTGGATGGCCTGCAAAGCATATCGGGATCTGGCCTTTGAGAAGGGGATCAAAACTCCAGA AATGTGGCTCCCCAAAGTGCCCATGCTGCATTTAACAAAGCAGCCAGTTACTTTGGGATG AAGATTGTGCGGGTCCCATTGACGAAGATGATGGAGGTGGATGTGCGGGCAATGAGAAGA GCTATCTCCAGGAACACTGCCATGCTCGTCTGTTCTACCCCACAGTTTCCTCATGGTGTA ATAGATCCTGTCCCTGAAGTGGCCAAGCTGGCTGTCAAATACAAAATACCCCTTCATGTC GACGCTTGTCTGGGAGGCTTCCTCATCGTCTTTATGGAGAAAGCAGGATACCCACTGGAG CACCCATTTGATTTCCGGGTGAAAGGTGTAACCAGCATTTCAGCTGACACCCATAAGTAT GGCTATGCCCCAAAAGGCTCATCATTGGTGTTGTATAGTGACAAGAAGTACAGGAACTAT CAGTTCTTCGTCGATACAGATTGGCAGGGTGGCATCTATGCTTCCCCAACCATCGCAGGC TCACGGCCTGGTGGCATTAGCGCAGCCTGTTGGGCTGCCTTGATGCACTTCGGTGAGAAC GGCTATGTTGAAGCTACCAAACAGATCATCAAAACTGCTCGCTTCCTCAAGTCAGAACTG GAAAATATCAAAGGCATCTTTGTTTTTGGGAATCCCCAATTGTCAGTCATTGCTCTGGGA TCCCGTGATTTTGACATCTACCGACTATCAAACCTGATGACTGCTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_003901 |
| Insert Size | 4500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003901.2, NP_003892.2 |
| RefSeq Size | 4701 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 8879 |
| UniProt ID | O95470 |
| Domains | pyridoxal_deC |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| Gene Summary | Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis (PubMed:11018465, PubMed:14570870, PubMed:24809814, PubMed:28165339). Required for global lipid homeostasis in liver and cholesterol homeostasis in fibroblasts. Involved in the regulation of pro-inflammatory response and neutrophil trafficking. Modulates neuronal autophagy via phosphoethanolamine production which regulates accumulation of aggregate-prone proteins such as APP (By similarity). Seems to play a role in establishing neuronal contact sites and axonal maintenance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208705 | SGPL1 (Myc-DDK-tagged)-Human sphingosine-1-phosphate lyase 1 (SGPL1) |
CNY 4,232.00 |
|
| RC208705L1 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), Myc-DDK-tagged |
CNY 6,632.00 |
|
| RC208705L2 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), mGFP tagged |
CNY 6,460.00 |
|
| RC208705L3 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), Myc-DDK-tagged |
CNY 6,632.00 |
|
| RC208705L4 | Lenti ORF clone of Human sphingosine-1-phosphate lyase 1 (SGPL1), mGFP tagged |
CNY 6,632.00 |
|
| RG208705 | SGPL1 (tGFP-tagged) - Human sphingosine-1-phosphate lyase 1 (SGPL1) |
CNY 5,832.00 |
