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DROSHA (NM_013235) Human Untagged Clone
CAT＃: SC308939
DROSHA (untagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1
CNY 26,510.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308939 representing NM_013235.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATGCAGGGAAACACATGTCACAGAATGTCGTTCCACCCGGGACGAGGGTGTCCCCGAGGACGAGGA GGACATGGAGCCAGACCCTCAGCACCATCCTTTAGGCCCCAAAATCTGAGGCTGCTTCACCCTCAGCAG CCTCCTGTGCAATATCAATATGAACCTCCAAGTGCCCCTTCCACCACTTTCTCAAACTCTCCAGCCCCC AATTTTCTCCCTCCACGACCAGACTTTGTACCCTTCCCCCCACCCATGCCTCCGTCAGCGCAAGGCCCT CTTCCCCCCTGCCCAATCAGGCCGCCTTTCCCCAACCACCAGATGAGGCACCCCTTCCCAGTTCCTCCT TGTTTTCCTCCCATGCCACCACCAATGCCTTGTCCTAATAACCCCCCAGTCCCTGGGGCACCTCCTGGA CAAGGCACTTTCCCCTTCATGATGCCCCCTCCCTCCATGCCTCATCCCCCGCCCCCTCCAGTCATGCCG CAGCAGGTTAATTATCAGTACCCTCCGGGCTATTCTCACCACAACTTCCCACCTCCCAGTTTTAATAGT TTCCAGAACAACCCTAGTTCTTTCCTGCCCAGTGCTAATAACAGCAGTAGTCCTCATTTCAGACATCTC CCTCCATACCCACTCCCAAAGGCTCCCAGTGAGAGAAGGTCCCCAGAAAGGCTGAAACACTATGATGAC CACAGGCACCGAGATCACAGTCATGGGCGAGGTGAGAGGCATCGGTCCCTGGATCGGCGGGAGCGAGGC CGCAGTCCCGACAGGAGAAGACAAGACAGCCGGTACAGATCTGATTATGACCGAGGGAGAACACCATCT CGCCACCGCAGCTACGAACGGAGCAGAGAGCGAGAACGGGAGAGACACAGGCATCGAGACAACCGAAGA TCACCATCTCTGGAAAGGTCCTACAAAAAAGAGTATAAGAGATCTGGAAGGAGTTACGGTTTATCGGTT GTTCCTGAACCTGCTGGATGCACACCAGAATTACCTGGGGAGATTATTAAAAATACAGATTCTTGGGCC CCACCCCTGGAGATTGTGAATCATCGCTCCCCAAGTAGGGAGAAGAAGAGAGCTCGTTGGGAGGAAGAA AAAGACCGTTGGAGTGACAACCAGAGTTCTGGCAAAGACAAGAACTATACCTCAATCAAGGAAAAAGAG CCCGAGGAGACCATGCCTGACAAGAATGAGGAGGAAGAAGAAGAACTTCTTAAGCCTGTGTGGATTCGA TGCACTCATTCAGAAAACTACTACTCCAGTGACCCCATGGATCAGGTGGGAGATTCTACAGTGGTTGGA ACGAGTAGGCTTCGTGACTTATATGACAAATTTGAGGAGGAGTTGGGGAGCAGGCAAGAAAAGGCCAAA GCTGCTCGGCCTCCGTGGGAACCTCCAAAGACGAAGCTCGATGAAGATTTAGAGAGTTCCAGTGAATCC GAGTGTGAGTCTGATGAGGACAGCACCTGTTCTAGCAGCTCAGACTCTGAAGTTTTTGACGTTATTGCA GAAATCAAACGCAAAAAGGCCCACCCTGACCGACTTCATGATGAACTTTGGTACAACGATCCAGGCCAG ATGAATGATGGACCACTCTGCAAATGCAGCGCAAAGGCAAGACGCACAGGAATTAGGCACAGCATTTAT CCTGGAGAAGAGGCCATCAAGCCCTGTCGTCCTATGACCAACAATGCTGGCAGACTTTTCCACTACCGG ATCACAGTCTCCCCGCCTACGAACTTTTTAACTGACAGGCCAACTGTTATAGAATACGATGATCACGAG TATATCTTTGAAGGATTTTCTATGTTTGCACATGCCCCCCTGACCAATATTCCACTGTGTAAAGTAATT AGATTCAACATAGACTACACGATTCATTTCATTGAAGAGATGATGCCGGAGAATTTTTGTGTGAAAGGG CTTGAACTCTTTTCACTGTTCCTATTCAGAGATATTTTGGAATTATATGACTGGAATCTTAAAGGTCCT TTGTTTGAAGACAGCCCTCCCTGCTGCCCAAGATTTCATTTCATGCCACGTTTTGTAAGATTTCTTCCA GATGGAGGAAAGGAAGTGCTGTCCATGCACCAGATTCTCCTGTACTTGTTAAGGTGCAGCAAAGCCCTG GTGCCTGAGGAGGAGATTGCCAATATGCTTCAGTGGGAGGAGCTGGAGTGGCAGAAATATGCAGAAGAA TGCAAAGGCATGATTGTTACCAACCCTGGGACGAAACCAAGCTCTGTCCGTATCGATCAACTGGATCGT GAACAGTTCAACCCCGATGTGATTACTTTTCCGATTATCGTCCACTTTGGGATACGCCCTGCACAGTTG AGTTATGCAGGAGACCCACAGTACCAAAAACTGTGGAAGAGTTATGTGAAACTTCGCCACCTCCTAGCA AATAGTCCCAAAGTCAAACAAACTGACAAACAGAAGCTGGCACAGAGGGAGGAAGCCCTCCAAAAAATA CGGCAGAAGAATACAATGAGACGAGAAGTAACGGTGGAGCTAAGTAGCCAAGGATTCTGGAAAACTGGC ATCCGTTCTGATGTCTGTCAGCATGCAATGATGCTACCTGTTCTGACCCATCATATCCGCTACCACCAA TGCCTAATGCATTTGGACAAGTTGATAGGATATACTTTCCAAGATCGTTGTCTGTTGCAGCTGGCCATG ACTCATCCAAGTCATCATTTAAATTTTGGAATGAATCCTGATCATGCCAGGAATTCATTATCTAACTGT GGAATTCGGCAGCCCAAATACGGAGACAGAAAAGTTCATCACATGCACATGCGGAAGAAAGGGATTAAC ACCTTGATAAATATCATGTCACGCCTTGGCCAAGATGACCCAACTCCCTCGAGGATTAACCACAATGAA CGGTTGGAATTCCTGGGTGATGCTGTTGTTGAATTTCTGACCAGCGTCCATTTGTACTATTTGTTTCCT AGTCTGGAAGAAGGAGGATTAGCAACCTATCGGACTGCCATTGTTCAGAATCAGCACCTTGCCATGCTA GCAAAGAAACTTGAACTGGATCGATTTATGCTGTATGCTCACGGGCCTGACCTTTGTAGAGAATCGGAC CTTCGACATGCAATGGCCAATTGTTTTGAAGCGTTAATAGGAGCTGTTTACTTGGAGGGAAGCCTGGAG GAAGCCAAGCAGTTATTTGGACGCTTGCTCTTTAATGATCCGGACCTGCGCGAAGTCTGGCTCAATTAT CCTCTCCACCCACTCCAACTACAAGAGCCAAATACTGATCGACAACTTATTGAAACTTCTCCAGTTCTA CAAAAACTTACTGAGTTTGAAGAAGCAATTGGAGTAATTTTTACTCATGTTCGACTTCTGGCAAGGGCA TTCACATTGAGAACTGTGGGATTTAACCATCTGACCCTAGGCCACAATCAGAGAATGGAATTCCTAGGT GACTCCATAATGCAACTGGTAGCCACAGAGTACTTATTCATTCATTTCCCAGATCATCATGAAGGACAC TTAACTTTGTTGCGAAGCTCTTTGGTGAATAATAGAACTCAGGCCAAGGTAGCGGAGGAGCTGGGCATG CAGGAGTACGCCATAACCAACGACAAGACCAAGAGGCCTGTGGCGCTTCGCACCAAGACCTTGGCGGAC CTTTTGGAATCATTTATTGCAGCGCTGTACATTGATAAGGATTTGGAATATGTTCATACTTTCATGAAT GTCTGCTTCTTTCCACGATTGAAAGAGTTCATTTTGAATCAGGATTGGAATGACCCCAAATCCCAGCTT CAGCAGTGTTGCTTGACACTTAGGACAGAAGGAAAAGAGCCAGACATTCCTCTGTACAAGACTCTGCAG ACAGTGGGCCCATCCCATGCCCGAACCTACACTGTGGCTGTTTATTTCAAGGGAGAAAGAATAGGCTGT GGGAAAGGACCAAGTATTCAGCAAGCGGAAATGGGAGCAGCAATGGATGCGCTTGAAAAATATAATTTT CCCCAGATGGCCCATCAGAAGCGGTTCATCGAACGGAAGTACAGACAAGAGTTAAAAGAAATGAGGTGG GAAAGAGAGCATCAAGAGAGAGAGCCAGATGAGACTGAAGACATCAAGAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_013235 |
| Insert Size | 4125 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013235.4 |
| RefSeq Size | 5424 bp |
| RefSeq ORF | 4125 bp |
| Locus ID | 29102 |
| UniProt ID | Q9NRR4 |
| Domains | RIBOc, DSRM |
| MW | 159.3 kDa |
| Gene Summary | This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213916 | DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 1 |
CNY 14,104.00 |
|
| RC213916L1 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged |
CNY 15,580.00 |
|
| RC213916L2 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, mGFP tagged |
CNY 15,580.00 |
|
| RC213916L3 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, Myc-DDK-tagged |
CNY 15,580.00 |
|
| RC213916L4 | Lenti ORF clone of Human drosha, ribonuclease type III (DROSHA), transcript variant 1, mGFP tagged |
CNY 15,580.00 |
|
| RG213916 | DROSHA (tGFP-tagged) - Human drosha, ribonuclease type III (DROSHA), transcript variant 1 |
CNY 15,010.00 |
