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YTHDC2 (NM_022828) Human Untagged Clone
CAT＃: SC308805
YTHDC2 (untagged)-Human YTH domain containing 2 (YTHDC2)
CN¥ 10,450.00
货期*
4周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAHL; hYTHDC2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308805 representing NM_022828.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCAGGCCGAGCAGCGTCTCCCCGCGGCAGCCGGCTCCTGGCGGTGGCGGAGGCGGCGGCCCCTCG CCTTGTGGCCCTGGGGGCGGCGGCCGGGCCAAGGGGCTGAAGGACATTCGCATTGATGAGGAGGTGAAG ATCGCAGTCAATATCGCGCTGGAGCGCTTCCGATACGGGGACCAGAGAGAAATGGAATTTCCTTCTTCT TTGACCAGTACTGAAAGAGCCTTTATTCATCGACTCAGTCAGTCTCTTGGTTTGGTCTCTAAAAGTAAA GGAAAGGGAGCAAATAGATACCTAACTGTGAAGAAGAAAGATGGATCAGAAACAGCTCATGCAATGATG ACCTGTAATTTGACTCATAATACAAAACATGCTGTTAGGAGCCTAATTCAAAGATTTCCTGTCACCAAT AAAGAGCGTACAGAACTTCTGCCTAAAACAGAAAGAGGAAATGTGTTTGCAGTTGAAGCTGAAAACCGG GAAATGAGCAAGACAAGTGGGCGACTCAACAATGGCATACCTCAGATTCCAGTGAAAAGAGGAGAATCC GAATTTGATTCTTTTAGGCAGTCTTTACCAGTGTTTGAGAAACAGGAAGAAATTGTTAAAATAATTAAG GAAAATAAAGTAGTTTTGATTGTAGGAGAAACTGGGTCTGGAAAGACCACACAGATTCCTCAGTTCCTT TTAGATGATTGCTTTAAAAATGGTATCCCCTGCCGTATATTTTGTACTCAACCAAGACGATTGGCAGCT ATCGCTGTGGCTGAAAGAGTTGCCGCAGAGAGACGGGAAAGGATTGGTCAAACAATTGGTTATCAGATC CGATTAGAAAGCAGGGTTTCTCCAAAGACACTTCTGACATTTTGTACTAATGGGGTATTGCTTCGTACA TTGATGGCAGGAGATAGTACGTTGTCGACTGTGACACATGTTATCGTGGATGAAGTGCATGAAAGGGAT CGATTTAGTGATTTTTTACTTACAAAGTTAAGAGATTTGTTGCAAAAGCACCCAACTTTGAAACTAATT CTTTCTAGTGCTGCCTTGGATGTAAATCTCTTTATAAGATATTTTGGAAGTTGTCCAGTGATATATATA CAGGGAAGACCATTTGAAGTAAAAGAAATGTTTCTGGAAGATATTTTAAGAACAACTGGATATACAAAC AAAGAAATGTTAAAATATAAAAAGGAAAAACAGCAAGAAGAGAAACAACAAACCACACTTACAGAATGG TACTCAGCTCAAGAAAATAGTTTCAAGCCTGAATCTCAGAGGCAGAGAACTGTTCTAAATGTGACTGAT GAGTATGACTTACTGGATGATGGTGGTGATGCTGTCTTCAGTCAGCTGACTGAAAAAGATGTGAATTGC CTTGAACCATGGTTAATAAAGGAAATGGATGCTTGCCTTTCTGATATATGGCTACATAAAGATATTGAT GCCTTTGCTCAGGTCTTTCATCTCATTTTAACTGAAAATGTTAGTGTTGATTACAGACATAGTGAAACC AGTGCAACAGCTCTGATGGTTGCTGCAGGACGTGGCTTTGCAAGTCAAGTAGAACAGTTAATCAGTATG GGAGCCAATGTCCATAGTAAAGCATCAAATGGCTGGATGGCATTGGATTGGGCTAAACACTTTGGGCAG ACTGAAATTGTGGATCTTCTAGAATCTTACAGTGCTACACTGGAATTTGGAAATCTAGATGAAAGTTCT CTGGTTCAAACAAATGGAAGTGACCTCAGTGCTGAAGACAGAGAGCTCCTGAAAGCTTATCATCATAGT TTCGATGATGAAAAAGTAGACTTGGATTTGATCATGCATCTTCTATACAATATCTGCCATAGTTGTGAT GCTGGTGCAGTACTAATTTTTCTGCCTGGATATGACGAAATTGTTGGACTGAGAGATCGCATCCTGTTT GATGACAAGCGGTTTGCTGACAGTACACATAGATACCAAGTCTTTATGCTTCATTCAAATATGCAAACA TCCGATCAAAAGAAAGTATTAAAAAACCCACCTGCAGGTGTTCGAAAAATAATTCTTTCCACCAATATT GCTGAAACCAGCATCACAGTCAATGATGTTGTCTTTGTTATTGATTCTGGTAAGGTGAAAGAGAAATCC TTTGATGCTCTGAATTTTGTTACAATGTTAAAAATGGTATGGATTTCCAAAGCTAGTGCCATACAGCGG AAAGGCAGGGCAGGGCGATGTAGACCTGGAATTTGTTTTCGTCTGTTCAGTAGACTCCGATTCCAGAAT ATGTTGGAATTTCAGACTCCGGAACTTTTGAGAATGCCATTACAGGAACTTTGCTTACATACCAAGCTG TTAGCCCCAGTTAATTGTCCCATTGCTGATTTTCTTATGAAAGCTCCTGAACCTCCACCAGCTTTAATT GTAAGAAATGCTGTACAAATGCTTAAGACAATAGATGCAATGGATACATGGGAAGATCTGACTGAACTT GGGTATCATTTGGCTGACTTGCCAGTAGAACCACATCTTGGTAAAATGGTCTTGTGTGCTGTTGTTTTA AAGTGTCTGGACCCCATCCTTACAATTGCTTGCACACTAGCTTATCGAGATCCTTTTGTACTACCTACT CAGGCCTCTCAAAAACGTGCAGCTATGCTTTGTAGGAAACGTTTTACTGCAGGAGCTTTCAGTGACCAT ATGGCACTTCTCAGAGCATTCCAGGCCTGGCAAAAAGCACGAAGTGATGGGTGGGAGCGAGCCTTTTGT GAAAAGAATTTTCTTTCACAGGCTACTATGGAAATAATCATAGGCATGAGAACACAGTTGCTTGGTCAA CTTAGAGCATCAGGTTTTGTTAGAGCACGAGGTGGTGGTGACATTCGGGACGTTAACACAAACTCTGAG AATTGGGCTGTCGTTAAAGCTGCATTGGTGGCAGGCATGTATCCTAATTTAGTCCACGTGGACAGAGAG AATCTAGTGTTGACAGGGCCAAAGGAGAAAAAAGTACGATTTCATCCTGCTTCAGTTCTCAGTCAGCCT CAATATAAAAAGATTCCTCCAGCCAATGGTCAAGCTGCAGCAATTAAGGCACTGCCCACAGATTGGCTT ATTTATGATGAAATGACCAGAGCCCATAGAATAGCTAATATTAGATGTTGTTCAGCAGTGACGCCTGTC ACTATATTGGTATTCTGTGGACCAGCTAGATTGGCAAGTAATGCTCTTCAGGAACCTTCATCCTTTAGA GTGGATGGCATTCCCAATGACAGTAGTGATAGTGAAATGGAGGACAAAACTACAGCTAATTTGGCAGCC TTGAAACTTGATGAGTGGCTCCATTTCACACTGGAGCCAGAGGCAGCTAGTTTATTGCTGCAGCTCAGA CAGAAGTGGCATAGCTTATTTTTACGCCGAATGAGAGCTCCATCTAAACCTTGGTCTCAAGTTGATGAA GCTACCATAAGAGCAATTATAGCTGTTTTAAGCACTGAAGAACAGTCTGCAGGTTTACAACAACCATCT GGGATTGGCCAAAGGCCAAGGCCTATGTCTTCAGAAGAGCTTCCTTTGGCCTCATCTTGGAGGTCAAAT AATAGTAGGAAAAGTTCAGCAGATACTGAATTTTCTGATGAGTGTACTACTGCAGAAAGAGTACTGATG AAATCTCCATCTCCAGCATTACACCCACCTCAGAAGTACAAAGATAGAGGAATTTTACATCCTAAACGA GGTACTGAGGACCGATCAGATCAGTCTTCTCTGAAATCTACAGACAGCAGTAGTTACCCAAGTCCTTGT GCTAGTCCTTCTCCTCCATCCTCAGGAAAGGGCTCAAAATCTCCTTCGCCAAGACCAAACATGCCTGTT CGATACTTCATAATGAAGAGTAGCAATTTGAGAAACCTTGAAATTTCTCAACAGAAGGGTATCTGGTCT ACAACTCCTAGTAATGAACGGAAGCTAAATCGAGCCTTTTGGGAAAGCAGCATAGTTTACTTGGTATTT TCTGTTCAAGGATCTGGACATTTCCAGGGATTTTCTAGGATGTCTTCTGAGATTGGAAGGGAAAAGAGT CAGGACTGGGGCTCTGCTGGACTAGGAGGAGTATTTAAGGTGGAGTGGATACGAAAAGAAAGCCTTCCC TTTCAATTTGCACACCATTTACTCAATCCATGGAATGACAACAAGAAAGTGCAGATAAGCAGGGATGGG CAGGAACTAGAACCTCTGGTTGGTGAACAGTTGCTCCAGTTATGGGAACGTCTTCCCTTGGGAGAAAAA AACACAACTGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022828 |
| Insert Size | 4293 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022828.3 |
| RefSeq Size | 6308 bp |
| RefSeq ORF | 4293 bp |
| Locus ID | 64848 |
| UniProt ID | Q9H6S0 |
| Domains | YTH, HA2 |
| MW | 160.2 kDa |
| Gene Summary | This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214383 | YTHDC2 (Myc-DDK-tagged)-Human YTH domain containing 2 (YTHDC2) |
CN¥ 14,672.00 |
|
| RC214383L3 | Lenti-ORF clone of YTHDC2 (Myc-DDK-tagged)-Human YTH domain containing 2 (YTHDC2) |
CN¥ 16,150.00 |
|
| RC214383L4 | Lenti-ORF clone of YTHDC2 (mGFP-tagged)-Human YTH domain containing 2 (YTHDC2) |
CN¥ 16,150.00 |
|
| RG214383 | YTHDC2 (tGFP-tagged) - Human YTH domain containing 2 (YTHDC2) |
CN¥ 16,272.00 |
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