PCDHA5 (NM_031501) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CNR6; CNRN6; CNRS6; CRNR6; PCDH-ALPHA5
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_031501, the custom clone sequence may differ by one or more nucleotides ATGGTATATTCCCGGAGAGGAAGTCTGGGATCCCGGCTCCTGCTGCTCTGGCTTCTCCTT GCCTACTGGAAGGCAGGGAGCGGCCAGCTCCACTACTCGATCCCGGAGGAAGCCAAACAC GGAACCTTCGTTGGCCGCATCGCGCAGGACCTAGGGCTGGAGCTGGCGGAGCTGGTGCCG CGCCTGTTCCGGGTGGCGTCCAAGGGCCGCGGGGACCTTCTGGAGGTAAATCTGCAGAAT GGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGGAGCTGTGCCGGCGGAGGGCGGAG TGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTTCCATGTGGAGGTG GCAGTGAAGGACATCAATGACAATCCGCCCAGGTTCTCCAGACAAGAACAAAGATTATTC ATTTTAGAGTCAAGAATGCCAGATTCGCGGTTTCCGCTAGAGGGCGCGTCGGATTTGGAT ATTGGAGCAAATGCACAATTGAGATACAGGTTAAATCCAAACGAATATTTTGACTTAGAT GTTAAAACAAATGAAGAAGAAACGAACTTTTTAGAGCTGGTTTTGAGGAAATCCTTAGAT AGAGAAGAAACACAAGAACACCGTTTATTAGTGATTGCAACTGATGGAGGAAAACCCGAA CTAACAGGTACAGTTCAGTTGTTGATCAATGTATTGGATGCTAATGATAACGCCCCAGAA TTTGATAAATCCATTTATAATGTCAGATTGTTGGAAAATGCACCAAGTGGGACATTAGTT ATTAAACTGAACGCCTCAGATGCAGATGAGGGCATCAATAAGGAAATAGTGTATTTCTTT AGTAATCTTGTTCTTGACGATGTAAAGTCCAAATTTATAATTAATTCTAATACTGGTGAA ATAAAAGTTAACGGGGAACTGGATTATGAAGACTATAACTCATATGAAATTAATATTGAT GCCATGGATAAAAGTACATTCCCATTATCAGGACACTGTAAAGTAGTAGTGAAACTCCTG GATGTGAATGATAATACCCCAGAGATGGCCATAACCACCCTTTTCCTGCCTGTCAAAGAG GACGCTCCACTCAGCACGGTCATTGCTCTGATCAGCGTGTCTGACCGTGACTCAGGTGCC AACGGGCAGGTGACCTGCTCCCTAATGCCCCACGTTCCCTTCAAGCTGGTGTCCACCTTC AAGAATTACTACTCGTTGGTGCTGGACAGCGCCCTGGACCGCGAGAGCGTGTCGGTCTAT GAGCTGGTGGTGACCGCGCGGGACGGGGGCTCGCCTTCGCTGTGGGCCACCGCCAGCGTG TCTGTGGAAGTGGCCGACGTGAACGACAACGCTCCGGCGTTCGCGCAGCCCCAGTATACC GTGTTCGTGAAGGAGAACAACCCGCCAGGCTGCCACATCTTCACGGTGTCTGCACGGGAC GCGGACGCGCAGGAGAACGCCCTGGTGTCCTACTCGCTGGTGGAGCGGCGGGTGGGCGAG CGCCCGCTGTCGAGTTACGTTTCGGTGCACGCGGAGAGCGGCAAGGTGTACGCGCTGCAG CCGCTGGACCACGAGGAAGTGGAGCTGCTGCAGTTCCAGGTGAGCGCGCGCGACGCGGGC GTGCCGCCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAGAACGACAAC GCGCCGGCGCTGCTGGTGCCTCGAGTGGGTGGCACCGGCGGCGCAGTGAGCGAGCTGGTG CCGAGGTCAGTGGGTGCGGGCCACGTGGTGGCGAAGGTGCGCGCAGTGGACCCTGATTCG GGCTACAACGCTTGGCTTTCGTATGAGCTGCAGCCAGCGCCTGGCAGTGCGCGCATCCCG TTCCGCGTGGGGCTGTACACAGGCGAGATCAGCACAACACGCTCTCTGGATGAGACCGAA GCACCGCGCCACCGCCTTCTGGTGCTGGTGAAGGACCATGGAGAGCCCCCGCTGACAGCC ACAGCCACAGTGCTGGTGTCGCTGGTGGAAAGTGGCCAGGCGCCGAAGGCCTCATCGCGG GCGTCGGCGGGCGCTGTGGGTCCCGAGGCTGCCCTGGTGGATGTCAACGTGTACCTGATC ATCGCCATCTGTGCGGTGTCCAGCCTGCTGGTGCTCACGCTGCTGCTGTACACCGCGCTG CGGTGCTCGGCGCAGCCCACCGAGGCCGTGTGCACACGGGGCAAGCCCACTCTGTTGTGC TCCAGCGCGGTGGGGAGCTGGTCGTACTCGCAGCAGAGGAGACAGAGGGTGTGCTCTGGG GAAGCTCCACCCAAAACAGACCTCATGGCCTTCAGTCCAAGCCTTCCTCAGGGTCCCACC TCTACAGACAACGTGAGTTTTCTAATATTAACATCCATCTTTCCCTCCCAATTTTCAAAT ATTAAATGTCACATTCACCCACTTTTTTTGTATTTAAAAATTATGTCTTAG
Restriction Sites	Please inquire
ACCN	NM_031501
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031501.1, NP_113689.1
RefSeq Size	2451 bp
RefSeq ORF	2451 bp
Locus ID	56143
UniProt ID	Q9Y5H7
Protein Families	Transmembrane
Gene Summary	This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes a shorter isoform (2), compared to isoform 1. This short variant is represented based on data in PMID:10380929.

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