United States
Germany
Japan
United Kingdom
China
FAM86A (EEF2KMT) (NM_201598) Human Untagged Clone
CAT＃: SC308071
FAM86A (untagged)-Human family with sequence similarity 86, member A (FAM86A), transcript variant 2
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | eEF2-KMT; EFM3; FAM86A; SB153 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC308071 representing NM_201598.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCCCGAGGAGAACGCGGGGACCGAACTCTTGCTGCAGAGTTTCGAGCGCCGCTTCCTGGCGGCA CGCACACTGCGCTCCTTCCCCTGGCAGAGCTTAGAAGCAAAGTTAAGAGACTCATCAGATTCTGAGCTG CTGCGGGATATTTTGCACAAGACTGTGAAGCATCCTGTGTGTGTGAAGCACCCGCCGTCCGTCAAATAT GCCCGGTGCTTTCTCTCAGAACTCATCAAAAAGCCCTCGGGAGGCTCGGTCACACTCTCCGAGAGCACG GCCATCATCTCCTACGGTACCACAGGCCTGGTCACATGGGACGCCGCCCTCTACCTTGCAGAATGGGCC ATCGAGAACCCGGCAGTCTTCACTAACAGGACTGTCCTAGAGCTTGGCAGTGGTGCTGGCCTCACAGGC CTGGCCATCTGCAAGATGTGCCGCCCCCGGGCATACATCTTCAGCGACTGTCACAGCCGGGTCCTTGAG CAGCTCCGAGGGAATGTCCTTCTCAATGGCCTCTCATTAGAGGCAGACATCACTGCCAAGTTAGACAGC CCCAGGGTGACAGTGGCCCAGCTGGACTGGGACGTCGCGACGGTCCATCAGCTCTCTGCCTTCCAGCCA GATGTTGTCATTGCAGCAGATGTGCTGTATTGCCCAGAAGCCATCATGTCGCTGGTCGGCGTCCTGCGG AGGCTGGCTGCCTGCCGGGAGCACCAGCGGGCTCCTGAGGTCTACGTGGCCTTTACCGTCCGCAACCCA GAGACGTGCCAGCTGTTCACCACCGAGCTAGGCCGGGCCGGGATCAGATGGGAAGTGGAACCTCGTCAT GAGCAGAAACTGTTTCCCTACGAAGAGCACTTGGAGATGGCAATGCTGAATCTCACCCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_201598 |
| Insert Size | 891 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_201598.3 |
| RefSeq Size | 2309 bp |
| RefSeq ORF | 891 bp |
| Locus ID | 196483 |
| UniProt ID | Q96G04 |
| MW | 33.1 kDa |
| Gene Summary | Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212357 | FAM86A (Myc-DDK-tagged)-Human family with sequence similarity 86, member A (FAM86A), transcript variant 2 |
CNY 2,400.00 |
|
| RC212357L3 | Lenti ORF clone of Human family with sequence similarity 86, member A (FAM86A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212357L4 | Lenti ORF clone of Human family with sequence similarity 86, member A (FAM86A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212357 | FAM86A (tGFP-tagged) - Human family with sequence similarity 86, member A (FAM86A), transcript variant 2 |
CNY 4,370.00 |
