United States
Germany
Japan
United Kingdom
China
GSDMA (NM_178171) Human Untagged Clone
CAT＃: SC307136
GSDMA (untagged)-Human gasdermin A (GSDMA)
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FKSG9; GSDM; GSDM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC307136 representing NM_178171.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCATGTTTGAAAATGTCACCCGGGCCCTGGCCAGACAGCTAAACCCTCGAGGGGACCTGACACCA CTTGACAGCCTCATCGACTTCAAGCGCTTCCATCCCTTCTGCCTGGTGCTGAGGAAGAGGAAGAGCACG CTCTTCTGGGGGGCCCGGTACGTCCGCACCGACTACACGCTGCTGGATGTGCTTGAGCCCGGCAGCTCA CCTTCAGACCCAACAGACACTGGGAATTTTGGCTTTAAGAATATGCTGGACACCCGAGTGGAGGGAGAT GTGGATGTACCAAAGACGGTGAAGGTGAAGGGAACGGCAGGGCTCTCGCAGAACAGCACTCTGGAGGTC CAGACACTCAGTGTGGCTCCCAAGGCCCTGGAGACCGTGCAGGAGAGGAAGCTGGCAGCAGACCACCCA TTCCTGAAGGAGATGCAAGATCAAGGGGAGAACCTGTATGTGGTGATGGAGGTGGTGGAGACGGTGCAG GAGGTCACACTGGAGCGAGCCGGCAAGGCAGAGGCCTGCTTCTCCCTCCCCTTCTTCGCCCCATTGGGG CTACAGGGATCCATAAATCACAAGGAGGCTGTAACCATCCCCAAGGGCTGCGTCCTGGCCTTTCGAGTG AGACAGCTGATGGTCAAAGGCAAAGATGAGTGGGATATTCCACATATCTGCAATGATAACATGCAAACC TTCCCTCCTGGAGAAAAGTCAGGAGAGGAGAAGGTCATCCTTATCCAGGCATCTGATGTTGGGGACGTA CACGAAGGCTTCAGGACACTAAAAGAAGAAGTTCAGAGAGAGACCCAACAAGTGGAGAAGCTGAGCCGA GTAGGGCAAAGCTCCCTGCTCAGCTCCCTCAGCAAACTTCTAGGGAAGAAAAAGGAGCTACAAGACCTT GAGCTCGCACTTGAAGGGGCTCTAGACAAGGGACATGAAGTGACCCTGGAGGCACTCCCAAAAGATGTC CTGCTATCAAAGGAGGCCGTGGGCGCCATCCTCTATTTCGTTGGAGCCCTAACAGAGCTAAGTGAAGCC CAACAGAAGCTGCTGGTGAAATCCATGGAGAAAAAGATCCTACCCGTGCAGCTAAAGCTGGTGGAGAGC ACGATGGAACAGAACTTCCTGCTGGATAAAGAGGGTGTTTTCCCCCTGCAACCTGAGCTGCTCTCCTCC CTTGGGGACGAGGAGCTGACCCTCACGGAGGCTCTAGTCGGGCTGAGTGGCCTGGAAGTGCAGAGATCG GGCCCCCAATATATGTGGGACCCAGACACCCTCCCTCGCCTCTGTGCTCTTTATGCAGGCCTCTCTCTC CTTCAGCAGCTTACCAAGGCCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_178171 |
| Insert Size | 1338 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178171.4 |
| RefSeq Size | 2164 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 284110 |
| UniProt ID | Q96QA5 |
| MW | 49.4 kDa |
| Gene Summary | May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219050 | GSDMA (Myc-DDK-tagged)-Human gasdermin A (GSDMA) |
CNY 3,656.00 |
|
| RC219050L1 | Lenti ORF clone of Human gasdermin A (GSDMA), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC219050L2 | Lenti ORF clone of Human gasdermin A (GSDMA), mGFP tagged |
CNY 5,890.00 |
|
| RC219050L3 | Lenti ORF clone of Human gasdermin A (GSDMA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219050L4 | Lenti ORF clone of Human gasdermin A (GSDMA), mGFP tagged |
CNY 5,890.00 |
|
| RG219050 | GSDMA (tGFP-tagged) - Human gasdermin A (GSDMA) |
CNY 5,256.00 |
