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GSDMA (NM_178171) Human Tagged ORF Clone
CAT＃: RC219050
- TrueORF®
GSDMA (Myc-DDK-tagged)-Human gasdermin A (GSDMA)
ORF Plasmid: tGFP
"NM_178171" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FKSG9; GSDM; GSDM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219050 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGTTTGAAAATGTCACCCGGGCCCTGGCCAGACAGCTAAACCCTCGAGGGGACCTGACACCAC TTGACAGCCTCATCGACTTCAAGCGCTTCCATCCCTTCTGCCTGGTGCTGAGGAAGAGGAAGAGCACGCT CTTCTGGGGGGCCCGGTACGTCCGCACCGACTACACGCTGCTGGATGTGCTTGAGCCCGGCAGCTCACCT TCAGACCCAACAGACACTGGGAATTTTGGCTTTAAGAATATGCTGGACACCCGAGTGGAGGGAGATGTGG ATGTACCAAAGACGGTGAAGGTGAAGGGAACGGCAGGGCTCTCGCAGAACAGCACTCTGGAGGTCCAGAC ACTCAGTGTGGCTCCCAAGGCCCTGGAGACCTTGCAGGAGAGGAAGCTGGCAGCAGACCACCCATTCCTG AAGGAGATGCAAGATCAAGGGGAGAACCTGTATGTGGTGATGGAGGTGGTGGAGACGGTGCAGGAGGTCA CACTGGAGCGAGCCGGCAAGGCAGAGGCCTGCTTCTCCCTCCCCTTCTTCGCCCCATTGGGGCTACAGGG ATCCATAAATCACAAGGAGGCTGTAACCATCCCCAAGGGCTGCGTCCTGGCCTTTCGAGTGAGACAGCTG ATGGTCAAAGGCAAAGATGAGTGGGATATTCCACATATCTGCAATGATAACATGCAAACCTTCCCTCCTG GAGAAAAGTCAGGAGAGGAGAAGGTCATCCTTATCCAGGCATCTGATGTTGGGGACGTACACGAAGGCTT CAGGACACTAAAAGAAGAAGTTCAGAGAGAGACCCAACAAGTGGAGAAGCTGAGCCGAGTAGGGCAAAGC TCCCTGCTCAGCTCCCTCAGCAAACTTCTAGGGAAGAAAAAGGAGCTACAAGACCTTGAGCTCGCACTTG AAGGGGCTCTAGACAAGGGACATGAAGTGAACCTGGAGGCACTCCCAAAAGATGTCCTGCTATCAAAGGA GGCCGTGGGCGCCATCCTCTATTTCGTTGGAGCCCTAACAGAGCTAAGTGAAGCCCAACAGAAGCTGCTG GTGAAATCCATGGAGAAAAAGATCCTACCCGTGCAGCTAAAGCTGGTGGAGAGCACGATGGAACAGAACT TCCTGCTGGATAAAGAGGGTGTTTTCCCCCTGCAACCTGAGCTGCTCTCCTCCCTTGGGGACGAGGAGCT GACCCTCACGGAGGCTCTAGTCGGGCTGAGTGGCCTGGAAGTGCAGAGATCGGGCCCCCAATATATGTGG GACCCAGACACCCTCCCTCGCCTCTGTGCTCTTTATGCAGGCCTCTCTCTCCTTCAGCAGCTTACCAAGG CCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219050 protein sequence
Red=Cloning site Green=Tags(s) MTMFENVTRALARQLNPRGDLTPLDSLIDFKRFHPFCLVLRKRKSTLFWGARYVRTDYTLLDVLEPGSSP SDPTDTGNFGFKNMLDTRVEGDVDVPKTVKVKGTAGLSQNSTLEVQTLSVAPKALETLQERKLAADHPFL KEMQDQGENLYVVMEVVETVQEVTLERAGKAEACFSLPFFAPLGLQGSINHKEAVTIPKGCVLAFRVRQL MVKGKDEWDIPHICNDNMQTFPPGEKSGEEKVILIQASDVGDVHEGFRTLKEEVQRETQQVEKLSRVGQS SLLSSLSKLLGKKKELQDLELALEGALDKGHEVNLEALPKDVLLSKEAVGAILYFVGALTELSEAQQKLL VKSMEKKILPVQLKLVESTMEQNFLLDKEGVFPLQPELLSSLGDEELTLTEALVGLSGLEVQRSGPQYMW DPDTLPRLCALYAGLSLLQQLTKAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178171 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178171.5 |
| RefSeq Size | 2164 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 284110 |
| UniProt ID | Q96QA5 |
| MW | 49.4 kDa |
| Gene Summary | May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219050L1 | Lenti ORF clone of Human gasdermin A (GSDMA), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC219050L2 | Lenti ORF clone of Human gasdermin A (GSDMA), mGFP tagged |
CNY 5,890.00 |
|
| RC219050L3 | Lenti ORF clone of Human gasdermin A (GSDMA), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219050L4 | Lenti ORF clone of Human gasdermin A (GSDMA), mGFP tagged |
CNY 5,890.00 |
|
| RG219050 | GSDMA (tGFP-tagged) - Human gasdermin A (GSDMA) |
CNY 5,256.00 |
|
| SC307136 | GSDMA (untagged)-Human gasdermin A (GSDMA) |
CNY 7,220.00 |
