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Dnmt2 (TRDMT1) (NM_176083) Human Untagged Clone
CAT＃: SC307060
TRDMT1 (untagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b
CNY 7,410.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DMNT2; DNMT2; M.HsaIIP; PuMet; RNMT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC307060 representing NM_176083.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCCCCTGCGGGTGCTGGAGCTATACAGCGGCGTGGGCGGCATGCACCACGCGCTGAGAGAAAGC TGTATACCTGCACAAGTGGTGGCTGCCATTGATGTCAACACTGTCGCTAATGAAGTATACAAGTATAAT TTTCCTCACACACAGTTACTTGCCAAGACGATTGAAGGCATTACACTCGAAGAGTTTGACAGATTATCT TTTGATATGATTTTAATGAGCCCTCCCTGCCAGCCATTCACAAGATTACAAAAATTACCAAAGTATATT CTTTTGGAAAATGTTAAAGGTTTTGAAGTATCTTCTACAAGAGACCTCTTGATACAAACAATAGAAAAT TGTGGCTTTCAGTACCAAGAGTTTCTATTATCTCCAACCTCTCTTGGCATTCCAAATTCAAGGCTACGA TATTTTCTTATTGCAAAGCTTCAGTCAGAGCCATTACCCTTTCAAGCCCCTGGTCAGGTACTGATGGAG TTCCCCAAAATTGAATCTGTACATCCACAAAAATATGCAATGGATGTAGAAAATAAAATTCAAGAAAAG AACGTTGAACCAAATATTAGCTTTGATGGCAGCATACAGTGTTCTGGAAAAGATGCCATTCTTTTTAAG CTTGAAACTGCAGAAGAAATTCACAGGAAAAATCAACAAGATAGTGATCTCTCTGTGAAAATGCTAAAA GATTTTCTTGAAGATGACACTGACGTGAACCAGTATCTTTTACCACCAAAGTCATTGCTGCGATATGCT CTTCTGTTAGACATTGTTCAGCCCACTTGTAGAAGGTCCGTGTGCTTTACCAAAGGATATGGAAGCTAC ATAGAAGGGACAGGGTCTGTGTTACAGACTGCAGAGGATGTGCAGGTTGAGAATATCTACAAATCCCTT ACCAATTTGTCACAAGAAGAACAGATAACAAAGCTGTTAATACTTAAACTGCGATATTTCACTCCTAAA GAAATAGCAAATCTCCTTGGATTTCCTCCAGAGTTCGGATTTCCTGAGAAGATAACAGTGAAACAGCGT TATCGCCTACTTGGAAATAGTCTCAACGTGCATGTAGTAGCTAAACTAATCAAAATCTTATATGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_176083 |
| Insert Size | 1104 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_176083.2 |
| RefSeq Size | 7209 bp |
| RefSeq ORF | 1104 bp |
| Locus ID | 1787 |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| MW | 41.9 kDa |
| Gene Summary | This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (b) lacks an alternate in-frame exon compared to variant a. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212492 | TRDMT1 (Myc-DDK-tagged)-Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b |
CNY 3,656.00 |
|
| RC212492L3 | Lenti ORF clone of Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212492L4 | Lenti ORF clone of Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b, mGFP tagged |
CNY 5,890.00 |
|
| RG212492 | TRDMT1 (tGFP-tagged) - Human tRNA aspartic acid methyltransferase 1 (TRDMT1), transcript variant b |
CNY 4,370.00 |
