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DNMT3B (NM_175850) Human Untagged Clone
CAT＃: SC307033
DNMT3B (untagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 6
CNY 16,340.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ICF; ICF1; M.HsaIIIB |
| Vector | pCMV6 series |
| Sequence Data |
>NCBI ORF sequence for NM_175850, the custom clone sequence may differ by one or more nucleotides
ATGGAACCAAGTCCTGAGCCTCCAAGCTTGGAAAGCATGAAGGGAGACACCAGGCATCTC AATGGAGAGGAGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCTGCAGC GACCAGTCCTCCGACTCGCCCCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGC CGAAGATCAAGCTCGCGACTCTCCAAGAGGGAGGTGTCCAGTCTGCTAAGCTACACACAG GACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCTCTGACACCCCAGTCATGCCA AAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAACTCGAAATAAC AACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCCGGCAG GGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGG CGGGCAACAGCATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCTCTTACCTTACCATC GACCTCACAGACGACACAGAGGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTAC GCCCGCCTAGCCCAGGACAGCCAGCAGGGGGGCATGGAGTCCCCGCAGGTGGAGGCAGAC AGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGGAGTTTGGAATAGGGGACCTC GTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTCTTGGAAGGCC ACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTGGGTCCAGTGGTTTGGCGATGGCAAG TTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAAT TTGGCCACCTTCAATAAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAA GCTAGGGTGCGAGCTGGCAAGACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGGACCAG CTGAAGCCCATGTTGGAGTGGGCCCACGGGGGCTTCAAGCCCACTGGGATCGAGGGCCTC AAACCCAACAACACGCAACCAGAGAACAAGACTCGAAGACGCACAGCTGACGACTCAGCC ACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAACAACGGCAAA GACCGAGGGGATGAAGATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACAACAAG AGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCT CTCTTTGAGGGGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATG TATGATGACGATGGCTATCAGTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCTGCTG CTTTGCAGCAACACGAGCTGCTGCCGGTGTTTCTGTGTGGAGTGCCTGGAGGTGCTGGTG GGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCTGGAGCTGTTACATGTGTCTC CCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCGCCTGCAGGCC TTCTTCACCAGTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCC GCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGGCTAC CTAGTCCTCAAAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAG GAGTCCATTGCTGTTGGAACCGTGAAGCACGAGGGGAATATCAAATACGTGAACGACGTG AGGAACATCACAAAGAAAAATATTGAAGAATGGGGCCCATTTGACTTGGTGATTGGCGGA AGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGAAAGGCCTGTATGAGGGTACA GGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAAGGAGGGTGAT GACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGG GACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCT GCTCACAGGGCCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCA TCAAAGAATGATAAACTCGAGCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGTTA AAGAAAGTACAGACAATAACCACCAAGTCGAACTCGATCAAACAGGGGAAAAACCAACTT TTCCCTGTTGTCATGAATGGCAAAGAAGATGTTTTGTGGTGCACTGAGCTCGAAAGGATC TTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGCCCGCCAGAAG CTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGAC TACTTTGCATGTGAATAG |
| Restriction Sites | Please inquire |
| ACCN | NM_175850 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_175850.1, NP_787046.1 |
| RefSeq Size | 4267 bp |
| RefSeq ORF | 2538 bp |
| Locus ID | 1789 |
| UniProt ID | Q9UBC3 |
| Protein Families | Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
| Gene Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011] Transcript Variant: This variant (6) uses an alternate exon in the 5' UTR and coding region that introduces an upstream start codon, compared to variant 1. Variant 6 also lacks an in-frame exon in the coding region, compared to variant 1. Isoform 6 is shorter and has a unique N-terminus, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222068 | DNMT3B (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 6 |
CNY 7,130.00 |
|
| RC222068L3 | Lenti-ORF clone of DNMT3B (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 6 |
CNY 9,030.00 |
|
| RC222068L4 | Lenti-ORF clone of DNMT3B (mGFP-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 6 |
CNY 9,030.00 |
|
| RG222068 | DNMT3B (tGFP-tagged) - Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 6 |
CNY 11,504.00 |
