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KSR2 (NM_173598) Human Untagged Clone
CAT＃: SC306873
KSR2 (untagged)-Human kinase suppressor of ras 2 (KSR2)
CNY 17,670.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306873 representing NM_173598.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATAGACTTGAGCATCTCCAACCTGGAAGGGCTTAGGACCAAATGTGCTACCTCCAACGACCTCACA CAAAAAGAAATCCGGACCCTGGAGAGCAAGCTGGTGAAGTACTTCAGCCGGCAGCTGTCCTGCAAAAAG AAGGTAGCCTTGCAGGAGCGCAACGCGGAGCTGGACGGCTTCCCCCAGCTACGGCACTGGTTCCGAATC GTCGATGTGCGCAAGGAGGTCCTGGAGGAAATCTCCCCCGGCCAGCTGAGCCTGGAGGACCTCTTGGAG ATGACGGATGAACAGGTGTGCGAGACTGTGGAGAAATACGGAGCCAACCGGGAGGAGTGTGCCCGCCTC AACGCCTCCCTCTCCTGCCTCAGGAATGTCCACATGTCAGGAGGCAACCTTTCCAAACAAGACTGGACC ATCCAGTGGCCCACGACAGAGACGGGGAAGGAGAACAATCCCGTGTGCCCCCCGGAGCCCACCCCGTGG ATCCGCACCCATCTCTCCCAGAGCCCCAGGGTCCCGTCCAAGTGCGTCCAGCACTATTGTCACACCAGC CCCACTCCCGGGGCCCCTGTGTACACCCACGTGGACAGGCTTACCGTGGACGCCTACCCGGGCTTGTGC CCGCCCCCGCCACTGGAGTCGGGCCACCGTTCCCTGCCCCCATCGCCCCGGCAGCGGCACGCGGTCCGC ACCCCGCCGCGCACCCCCAACATCGTCACCACCGTGACCCCGCCGGGCACGCCGCCCATGAGGAAGAAG AACAAGCTGAAGCCCCCGGGGACCCCACCGCCCTCCTCCCGAAAACTGATACACTTGATCCCGGGATTC ACCGCGCTGCATCGGAGCAAATCCCACGAGTTCCAGCTGGGGCACCGCGTGGACGAGGCCCACACGCCC AAAGCCAAGAAGAAGAGCAAACCCTTGAACCTCAAGATCCACAGCAGCGTAGGCAGCTGCGAGAACATC CCCTCTCAGCAGCGCTCCCCGCTGCTGTCCGAGCGCTCCCTCCGCTCCTTCTTTGTGGGACACGCACCT TTCCTGCCTTCCACCCCTCCTGTTCACACTGAGGCCAACTTCTCTGCAAACACACTGTCAGTGCCACGC TGGTCCCCGCAGATCCCTCGCAGAGATCTCGGCAACTCCATCAAGCACAGGTTTTCCACCAAGTACTGG ATGTCTCAGACGTGCACAGTCTGTGGGAAAGGGATGCTTTTTGGCCTCAAGTGTAAAAACTGCAAGTTA AAGTGCCACAACAAATGCACCAAAGAAGCCCCACCCTGTCATCTTCTGATCATCCACCGAGGAGATCCA GCAAGGTTAGTCCGGACAGAGTCCGTTCCGTGTGACATCAACAACCCTCTACGGAAGCCACCTCGCTAT TCAGACCTGCACATCAGTCAGACGCTCCCCAAAACCAACAAAATCAACAAGGACCACATCCCTGTCCCT TACCAGCCAGACTCCAGCAGCAACCCCTCCTCCACGACGTCCTCCACGCCCTCCTCGCCAGCACCCCCC CTCCCTCCTAGTGCCACGCCGCCTTCTCCCCTACACCCTTCCCCACAGTGCACACGGCAGCAGAAGAAC TTCAACCTGCCAGCATCCCACTACTACAAATACAAGCAGCAGTTCATCTTCCCAGATGTGGTGCCGGTG CCGGAGACGCCGACCCGGGCGCCCCAGGTCATCCTGCATCCGGTGACCTCGAATCCAATCTTGGAAGGA AATCCATTACTTCAAATTGAAGTGGAGCCAACGTCGGAGAATGAAGAGGTCCATGATGAGGCCGAAGAG TCAGAGGATGACTTCGAGGAGATGAACCTGTCCCTCCTCTCGGCCCGGAGCTTCCCACGCAAGGCCAGC CAGACCAGCATCTTCCTTCAGGAGTGGGACATCCCCTTTGAGCAGCTGGAGATCGGCGAGCTCATTGGA AAGGGCCGCTTTGGGCAAGTGTACCACGGCCGCTGGCATGGCGAGGTGGCCATCCGGCTGATTGACATT GAGAGGGACAACGAGGACCAGCTCAAGGCCTTCAAGCGGGAGGTGATGGCCTACAGGCAGACACGGCAT GAGAACGTGGTGCTTTTCATGGGTGCCTGCATGAGCCCGCCTCACCTGGCCATCATCACCAGCCTCTGT AAGGGACGGACGCTCTATTCCGTTGTGAGGGATGCCAAAATCGTTTTGGATGTCAACAAAACCAGGCAG ATTGCTCAAGAAATTGTGAAGGGCATGGGCTACCTCCACGCCAAGGGAATCCTACACAAGGACCTCAAG TCAAAGAACGTCTTCTATGACAACGGCAAAGTGGTCATCACGGACTTTGGACTCTTCAGCATTTCTGGG GTGCTGCAGGCTGGCAGGCGGGAGGACAAACTGCGCATCCAGAATGGCTGGCTATGCCACCTGGCACCA GAGATCATCCGCCAGCTGTCCCCCGACACAGAGGAGGATAAGCTCCCCTTCTCCAAGCACTCTGACGTC TTTGCCCTTGGCACAATCTGGTATGAACTCCACGCCAGGGAATGGCCTTTCAAGACCCAACCAGCAGAG GCAATAATCTGGCAAATGGGCACAGGCATGAAACCCAACCTCAGCCAGATTGGCATGGGAAAAGAAATC TCGGACATTCTTCTCTTCTGCTGGGCCTTTGAACAAGAAGAGAGACCTACCTTCACCAAGCTCATGGAC ATGCTGGAGAAACTGCCAAAGCGAAACCGTCGCCTGTCTCACCCTGGACATTTCTGGAAGTCTGCAGAG CTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_173598 |
| Insert Size | 2766 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173598.4 |
| RefSeq Size | 17008 bp |
| RefSeq ORF | 2766 bp |
| Locus ID | 283455 |
| UniProt ID | Q6VAB6 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 104.3 kDa |
| Gene Summary | Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211665 | KSR2 (Myc-DDK-tagged)-Human kinase suppressor of ras 2 (KSR2) |
CNY 6,744.00 |
|
| RC211665L1 | Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), Myc-DDK-tagged |
CNY 9,144.00 |
|
| RC211665L2 | Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), mGFP tagged |
CNY 9,030.00 |
|
| RC211665L3 | Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), Myc-DDK-tagged |
CNY 9,144.00 |
|
| RC211665L4 | Lenti ORF clone of Human kinase suppressor of ras 2 (KSR2), mGFP tagged |
CNY 9,144.00 |
|
| RG211665 | KSR2 (tGFP-tagged) - Human kinase suppressor of ras 2 (KSR2) |
CNY 7,890.00 |
|
| SC318393 | KSR2 (untagged)-Human kinase suppressor of ras 2 (KSR2) |
CNY 6,752.00 |
