ABCA12 (NM_173076) Human Untagged Clone

CAT#: SC306782

ABCA12 (untagged)-Human ATP-binding cassette, sub-family A (ABC1), member 12 (ABCA12), transcript variant 1



  "NM_173076" in other vectors (3)

CNY 17,136.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-ABCA12 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ARCI4A; ARCI4B; ICR2B; LI2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_173076 edited
ACTGATACACTGACATACAGCAAGTTGGACGGGGCATCAGTTCTTCATTTGTGGAGTGGA
GAAAAGAAGAGGAAATCTCTCATTTGGGGCATTTGAAGGATGGCTTCCCTGTTTCATCAG
CTTCAGATCCTGGTCTGGAAAAATTGGCTAGGTGTAAAAAGGCAGCCGCTTTGGACACTT
GTCTTGATCTTATGGCCAGTCATTATTTTCATAATTTTGGCTATTACTCGGACCAAATTT
CCTCCAACTGCAAAACCAACTTGTTACCTCGCACCTCGAAACCTTCCTAGTACTGGATTC
TTTCCATTCCTGCAGACCCTACTCTGTGACACAGACTCTAAATGCAAAGACACACCCTAT
GGCCCACAAGATCTGCTTCGTAGGAAAGGAATTGATGATGCACTATTTAAAGACAGTGAG
ATTCTGAGAAAGTCATCCAACCTGGATAAGGACAGCAGTTTATCATTCCAGAGCACCCAA
GTTCCAGAAAGAAGGCATGCATCACTAGCCACAGTATTTCCCAGTCCAAGTTCTGATTTG
GAAATCCCCGGAACATATACTTTCAATGGCAGTCAAGTGCTCGCACGAATTCTTGGCTTG
GAAAAGCTGTTAAAGCAAAATTCAACTTCAGAAGATATACGAAGAGAACTATGTGACAGC
TATTCAGGATACATTGTGGATGATGCCTTCTCTTGGACCTTTCTAGGAAGAAATGTTTTT
AACAAATTTTGCCTTTCTAACATGACCCTTTTAGAGTCTTCTCTCCAAGAACTAAACAAA
CAGTTCTCCCAGCTATCCAGTGACCCCAACAATCAGAAGATAGTGTTTCAGGAAATAGTC
AGAATGCTGTCTTTCTTCTCACAAGTGCAAGAGCAGAAAGCTGTGTGGCAGCTTCTGTCT
AGTTTTCCAAATGTGTTTCAGAATGACACATCACTAAGCAATCTATTTGATGTTCTTCGA
AAGGCAAACAGTGTGCTGCTGGTTGTGCAGAAGGTTTATCCACGTTTTGCAACTAACGAA
GGTTTCAGAACCCTCCAGAAGTCTGTTAAACATCTGCTGTACACTCTGGACTCCCCAGCT
CAAGGTGACTCCGATAATATAACGCATGTGTGGAATGAGGATGATGGACAGACCTTATCT
CCAAGCAGTCTGGCTGCACAGCTCCTAATTCTGGAAAACTTTGAAGATGCCCTCTTAAAT
ATATCAGCAAATAGTCCTTATATTCCTTACTTGGCATGTGTGAGAAATGTGACTGACAGT
TTGGCCAGAGGTTCACCAGAAAATCTAAGACTCCTGCAGTCCACAATACGATTTAAAAAA
TCTTTTCTTCGCAATGGTTCCTATGAAGATTACTTTCCTCCAGTTCCTGAAGTCCTAAAA
TCAAAACTGTCTCAACTTCGAAACTTGACCGAACTTCTTTGTGAATCTGAAACTTTCAGT
TTGATAGAGAAGTCATGCCAGCTCTCTGATATGAGCTTTGGGAGCCTGTGTGAAGAAAGT
GAGTTTGATCTGCAACTCCTCGAAGCGGCAGAGCTGGGCACCGAAATAGCAGCCAGCTTA
CTGTACCATGACAATGTCATATCTAAAAAAGTGAGAGATTTGCTGACTGGAGATCCAAGC
AAAATTAATTTAAATATGGATCAGTTTCTAGAACAGGCACTGCAAATGAATTACTTGGAA
AATATCACTCAGTTAATACCGATCATAGAAGCCATGCTGCATGTCAATAACAGTGCAGAT
GCTTCTGAAAAGCCAGGTCAGTTACTAGAAATGTTTAAAAATGTTGAAGAGCTGAAAGAA
GATTTAAGGAGAACAACAGGAATGTCCAACAGGACTATTGACAAGTTGCTGGCCATTCCC
ATCCCTGATAATAGAGCTGAGATTATTTCTCAGGTGTTCTGGCTGCATTCCTGTGATACT
AATATCACCACTCCCAAACTAGAAGATGCAATGAAAGAATTCTGCAACCTGTCTCTTTCA
GAGAGATCCCGGCAGTCTTACCTCATCGGACTCACCCTTCTGCACTACTTAAACATTTAC
AACTTCACATACAAGGTGTTTTTCCCGAGGAAAGATCAAAAGCCAGTAGAAAAGATGATG
GAGCTCTTCATAAGACTAAAAGAGATTCTCAATCAGATGGCTTCTGGCACACATCCGCTG
CTAGACAAAATGAGATCCCTGAAGCAAATGCATCTGCCCAGAAGTGTTCCATTAACACAG
GCAATGTACAGAAGCAACCGAATGAACACACCACAAGGATCATTTAGCACCATCTCCCAA
GCATTATGTTCTCAAGGAATTACCACTGAATATTTAACTGCCATGCTGCCCTCTTCCCAG
AGGCCAAAAGGCAACCACACCAAGGATTTTTTGACTTATAAATTAACTAAAGAGCAAATT
GCTTCAAAATATGGAATTCCCATAAATACCACACCATTTTGCTTCTCCCTTTATAAAGAC
ATCATTAACATGCCCGCTGGACCTGTGATTTGGGCTTTCTTGAAACCTATGTTGTTGGGA
AGAATTTTGTATGCACCATATAACCCAGTCACAAAGGCAATAATGGAAAAGTCCAATGTA
ACTCTGAGACAGCTGGCGGAATTAAGAGAAAAATCTCAAGAGTGGATGGATAAGTCGCCA
CTTTTCATGAATTCCTTCCATCTGTTAAACCAGGCAATTCCAATGCTCCAGAATACTCTA
AGGAACCCTTTTGTGCAAGTTTTTGTAAAGTTCTCCGTGGGACTCGATGCTGTTGAACTA
TTGAAACAGATAGATGAACTCGATATTCTAAGACTGAAATTAGAGAACAACATTGACATC
ATCGATCAGCTTAACACACTATCTTCCCTGACAGTAAATATTTCCTCTTGTGTATTATAT
GACCGTATTCAGGCAGCAAAAACCATAGATGAAATGGAGAGAGAGGCTAAAAGGCTCTAC
AAAAGCAACGAACTCTTTGGAAGTGTTATTTTTAAGCTTCCTTCTAACAGAAGCTGGCAC
AGAGGCTATGACTCTGGAAATGTCTTTCTTCCTCCTGTCATAAAATATACCATCCGGATG
AGTCTCAAGACCGCACAGACCACAAGAAGCCTAAGAACCAAGATTTGGGCTCCAGGGCCA
CACAATTCTCCGTCACACAACCAGATCTATGGCAGGGCTTTTATTTATTTACAGGATAGT
ATTGAAAGAGCAATCATTGAATTGCAAACTGGAAGGAACTCCCAGGAAATAGCAGTCCAG
GTTCAAGCAATTCCTTATCCCTGCTTCATGAAAGACAACTTCCTAACCAGTGTCTCTTAT
TCTCTTCCAATTGTGCTTATGGTTGCCTGGGTTGTATTTATAGCTGCCTTTGTAAAAAAG
CTTGTCTATGAGAAAGACCTCCGGCTTCATGAGTACATGAAGATGATGGGTGTGAACTCC
TGCAGCCATTTCTTTGCCTGGCTTATAGAGAGTGTTGGATTTTTACTGGTTACCATCGTG
ATCCTCATCATTATACTCAAGTTTGGCAATATTCTTCCTAAAACAAATGGGTTCATTTTG
TTCCTGTATTTTTCGGACTACAGCTTCTCGGTTATTGCCATGAGCTATCTTATCAGTGTC
TTCTTCAACAACACCAACATTGCAGCTCTGATCGGAAGCCTCATCTACATCATTGCCTTC
TTTCCATTTATTGTTCTGGTTACAGTGGAGAATGAGTTGAGCTATGTATTGAAAGTGTTC
ATGAGCCTGCTGTCCCCAACAGCATTCAGCTATGCAAGCCAATACATTGCACGATACGAA
GAACAGGGCATTGGTCTTCAGTGGGAAAATATGTACACCTCCCCGGTTCAGGATGACACC
ACCTCATTTGGCTGGCTGTGCTGTCTAATCCTAGCTGACTCTTTCATTTATTTCCTTATT
GCTTGGTATGTCAGGAATGTCTTCCCAGGGACATACGGTATGGCAGCTCCCTGGTATTTT
CCAATTCTTCCTTCCTATTGGAAGGAGCGATTTGGGTGTGCAGAGGTGAAGCCTGAGAAG
AGCAATGGCCTCATGTTTACTAACATCATGATGCAGAACACCAACCCATCTGCCAGTCCT
GAATACATGTTTTCCTCTAACATCGAGCCTGAACCTAAAGATCTCACAGTCGGGGTTGCC
CTGCATGGGGTCACAAAGATCTATGGCTCAAAAGTTGCTGTTGATAACCTCAATCTGAAC
TTTTATGAAGGGCATATTACTTCATTGCTGGGGCCCAATGGAGCTGGGAAAACTACTACC
ATTTCCATGTTAACTGGGCTGTTTGGGGCCTCAGCAGGCACCATTTTTGTATATGGAAAA
GATATCAAAACAGACCTACACACGGTACGGAAGAACATGGGAGTCTGTATGCAGCACGAC
GTCTTGTTCAGTTACCTCACTACTAAGGAGCACCTTCTCCTATATGGTTCCATCAAAGTT
CCTCACTGGACTAAAAAGCAGCTCCACGAGGAAGTAAAAAGGACTTTAAAAGATACTGGA
CTATATAGCCATCGTCATAAGAGAGTTGGAACACTGTCAGGAGGCATGAAGAGGAAGTTA
TCTATATCCATAGCTCTCATTGGTGGATCAAGGGTAGTAATTTTGGATGAACCATCTACT
GGAGTTGACCCATGTTCTCGCCGAAGTATATGGGATGTTATATCCAAGAACAAAACTGCC
AGAACAATCATTCTGTCAACGCACCACTTGGACGAGGCTGAAGTGCTGAGTGACCGCATC
GCCTTCCTGGAGCAGGGTGGGCTTAGGTGCTGTGGGTCCCCATTTTACCTCAAGGAAGCC
TTTGGCGATGGGTATCACCTCACGCTTACCAAGAAGAAGAGTCCAAATTTAAATGCAAAT
GCAGTATGTGACACCATGGCCGTGACAGCAATGATCCAATCACATCTCCCCGAAGCCTAC
CTCAAGGAGGATATTGGGGGAGAGCTTGTTTATGTACTTCCTCCATTCAGCACCAAAGTC
TCAGGGGCCTACCTGTCACTCCTACGGGCACTCGACAATGGCATGGGTGACCTCAACATC
GGGTGCTACGGCATTTCAGATACCACCGTGGAGGAGGTCTTTCTGAACTTGACCAAAGAG
TCACAAAAAAATAGTGCTATGAGTCTTGAGCACTTAACACAAAAGAAAATTGGGAATTCC
AATGCCAATGGCATCTCAACTCCTGACGATTTATCTGTGAGCAGCAGCAATTTCACAGAC
AGAGATGACAAAATCCTGACAAGAGGAGAGAGGCTGGATGGCTTTGGACTGTTGCTGAAG
AAGATCATGGCTATACTCATCAAGAGGTTCCACCACACCCGCAGGAACTGGAAAGGTCTC
ATTGCTCAGGTTATCCTCCCCATCGTCTTTGTTACCACTGCCATGGGCCTTGGCACACTG
AGAAATTCCAGCAACAGTTATCCAGAGATTCAGATCTCCCCCTCTCTTTATGGTACCTCC
GAACAGACAGCCTTCTATGCTAATTATCACCCGAGCACGGAAGCACTTGTCTCAGCAATG
TGGGACTTCCCTGGAATTGACAACATGTGTCTGAACACCAGTGATCTACAGTGTTTAAAC
AAAGACAGTCTGGAAAAATGGAACACCAGTGGAGAACCCATCACTAATTTTGGTGTTTGC
TCCTGCTCAGAAAATGTCCAGGAATGTCCTAAATTTAACTATTCCCCACCGCACAGAAGA
ACTTACTCATCCCAGGTAATTTATAACCTCACTGGGCAACGAGTGGAAAATTATCTTATA
TCAACTGCAAATGAGTTTGTCCAAAAAAGATATGGAGGTTGGAGTTTTGGGCTGCCTTTG
ACAAAAGACCTTCGTTTTGATATAACAGGAGTCCCTGCCAATAGAACACTTGCCAAGGTA
TGGTATGATCCAGAAGGCTATCACTCCCTTCCAGCTTACCTCAACAGCCTGAATAATTTC
CTTCTGCGAGTTAACATGTCAAAATACGATGCTGCCCGACATGGCATCATCATGTATAGC
CATCCTTATCCAGGAGTGCAAGACCAAGAACAAGCCACAATCAGCAGTTTAATCGATATT
TTAGTGGCACTGTCTATCTTGATGGGCTACTCTGTCACCACCGCCAGCTTTGTCACCTAT
GTTGTAAGGGAACATCAAACCAAAGCCAAACAGTTGCAGCACATTTCAGGCATTGGCGTG
ACATGCTACTGGGTAACAAACTTCATTTATGACATGGTTTTCTACTTGGTGCCTGTAGCG
TTTTCAATTGGTATCATTGCGATTTTCAAATTACCTGCATTCTACAGTGAAAACAACCTA
GGCGCTGTATCTCTCCTACTTCTCCTGTTTGGGTATGCAACATTTTCCTGGATGTACTTG
CTGGCTGGGCTCTTCCATGAAACAGGAATGGCCTTCATCACTTACGTCTGTGTCAACTTG
TTTTTTGGCATTAATTCCATTGTTTCCCTGTCAGTGGTATACTTTCTTTCCAAGGAAAAG
CCTAATGATCCGACTTTAGAACTTATTTCTGAAACCCTCAAGCGCATTTTCCTGATTTTC
CCACAATTCTGTTTTGGCTACGGTTTGATTGAACTTTCTCAACAACAGTCGGTCCTAGAC
TTCTTAAAAGCATATGGAGTGGAATACCCAAATGAAACCTTTGAGATGAATAAACTAGGT
GCAATGTTTGTGGCTTTGGTTTCTCAGGGCACCATGTTTTTTTCCTTGCGACTCTTAATC
AACGAATCCCTGATAAAGAAACTCAGGCTTTTCTTCAGAAAATTTAATTCTTCACATGTA
AGGGAGACAATAGATGAGGATGAAGATGTGCGGGCTGAGAGATTAAGAGTTGAGAGTGGT
GCAGCTGAATTTGACTTGGTCCAACTTTATTGTCTCACAAAGACCTACCAACTTATCCAC
AAAAAGATTATAGCTGTAAACAACATCAGCATTGGGATACCTGCTGGAGAGTGTTTTGGG
CTTCTTGGAGTGAATGGAGCAGGAAAGACCACTATATTCAAGATGCTGACAGGAGACATC
ATTCCTTCAAGTGGAAACATTCTGATCAGAAATAAGACCGGATCTCTGGGTCACGTTGAT
TCTCACAGCTCATTAGTTGGCTACTGTCCTCAGGAAGATGCCTTAGATGACCTGGTAACT
GTGGAAGAACATTTGTATTTCTATGCCAGGGTACATGGAATTCCAGAAAAGGATATTAAA
GAAACTGTTCATAAACTCCTTAGGAGACTTCACCTGATGCCCTTCAAGGACAGAGCTACC
TCTATGTGCAGTTATGGCACAAAAAGAAAATTATCCACTGCACTGGCCTTGATAGGGAAA
CCTTCCATTCTACTGCTGGATGAGCCGAGCTCTGGCATGGATCCGAAGTCGAAACGGCAC
CTCTGGAAGATCATTTCAGAAGAAGTACAGAACAAATGTTCCGTCATCCTCACATCTCAC
AGCATGGAAGAATGTGAAGCTCTCTGTACCAGGTTGGCCATTATGGTGAATGGAAAGTTT
CAATGTATTGGATCTTTGCAGCACATAAAGAGCAGGTTTGGACGAGGATTTACTGTCAAA
GTTCACTTGAAGAATAACAAAGTGACCATGGAGACCCTCACAAAGTTCATGCAGCTGCAC
TTTCCAAAAACATACTTAAAAGATCAGCACCTCAGCATGCTAGAGTATCATGTACCAGTC
ACAGCAGGAGGAGTCGCAAACATTTTTGATCTGCTGGAAACCAACAAGACTGCTTTAAAT
ATTACAAATTTCTTAGTGAGTCAGACCACTCTGGAAGAGGTTTTCATCAACTTTGCCAAA
GACCAGAAGTCCTATGAAACTGCTGATACCAGCAGCCAAGGTTCCACTATAAGTGTTGAC
TCACAAGATGACCAGATGGAGTCTTAACACTTCCAGCAAACTCAATCTCAGCATGTGACC
AATGGCTTCATTTTGAAGAAAAGCCACAGAAGATACACTTCCGCAAGATATCTTCATTTT
AAAGTAA
>OriGene 5' read for NM_173076 unedited
GGGGTTCACGATTTTGTATACGACTCACTATAGGGCGGCTCGCGAATTCGCACGAGGTCA
CTGCAGATATCGCTCCACTTTTCCCACCGGTTGGTGCCCTGAGGGGCCCACATTTATGGG
CCACTTCAGTGATTTTTTTTTGGCTTCCATGGGCTCCTACTGCTTTCAGCCAGTGAGGAT
CAATGGCAGGAGAGGGAGGAGAGCAGACAGAGGGAGGAGAAAGTGAGGCATTATCTGAAT
GATGCTCATTCTCTCTTGAGTCTGGACTTCGTGCAACAAGCAGCAACTGGATATTATTTT
AAAATAAGCATAAGCCACCCAAAGATACTGATCTGGGTCCTTCTTTTGGAAGAAGAGTTG
ATTGAGAAGTGCCTCTTGGTTAAGGATTAACCACAGGGAAAAATCCAGCAGAAACAGAAG
AACTGTGGGTTTCTTACCCCAGCCCTCAAGGAAGCTATGCCGTGAAAGGGGTACTGATAC
ACTGACATACAGCAAGTTGGACGGGGCATCAGTTCTTCATTTGTGGAGTGGAGAAAAGAA
GAGGAAATCTCTCATTTGGGGCATTTGAAGGATGGCTTCCCTGTTTCATCAGCTTCAGAT
CCTGGTCTGGAAAAATTGGCTAGGTGTAAAAAGGCAGCCGCTTTGGACACTTGTCTTGAT
CTTATGGCCAGTCATTATTNTCATAATTTTGGCTATTACTCGGACCAAATTTCCCTCCAC
TGCAAAACCAACTTGNTACCTCGCACCTCGAAACCTTCCTAGTACTGGATTCTTTTCATT
CCTGCAGACCCTACTCTGTGACACAGACTCTAAATGGCAAGACCCACCCTATGGCCCACA
AGATCTGCTTCGTAGGAAAGGAATTGATGATGCCCTATTTAAGACAGTGAGATCTGAA
>OriGene 3' read for NM_173076 unedited
NCCATTGGAATGGCACTTCCAGGNCCAGAAAAGCACTGGGGNAGGGTCACAGGGATGCCA
CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTT
TTTTTTTTTTTTCCTTTTAAACTGTCTTTTTATTGAAAGTAATAAATTAAGATATTCATC
TTGAGGTGGCTTCACCTTTGCATAAGAATCACCAGCATATACATTAGCATGCTCACAGTG
TTGAGTATACAGGAATTCATTTCAGTAGCAACAACACTCACTGACCTTAGAAGGAAAAAT
TTCCTTTTATAATTACTTGTTAGTCTAACACAGTTGTAACTTTCCATACAGTATATTACT
TTACTTTAAAATGAAGATATCTTGCGGAAGTGTATCTTCTGTGGCTTTTCTTCAAAATGA
AGCCATTGGTCACATGCTGAGATTGAGTTTGCTGGAAGTGTTAAGACTCCATCTGGTCAT
CTTGTGAGTCAACACTTATAGTGGAACCTTGGCTGCTGGTATCAGCAGTTTCATAGGACT
TCTGGTCTTTGGCAAAGTTGATGAAAACCTCTTCCAGAGTGGTCTGACTCACTAAGAAAT
TTGTAATATTTAAAGCAGTCTTGTTGGTTTCCAGCAGATCAAAAATGTTTGCGACTCCTC
CTGCTGTGACTGGTACATGATACTCTAGCATGCTGAGGTGCTGATCTTTTAAGTATGTTT
TTGGAAAGTGCAGCTGCATGAACTTTGTGAGGGTCTCCATGGTCACTTTGTTATTCTCCA
ATGAACTTTGACAGTAAATCCTCGTCCAAACCTGCTCTTTATGTGCTGCAAAGATCCATA
CATTGAAACTTTCCATTCACCAATATGGCCAACCTGTTACAGAGAGCTTCACATTCTTCC
Restriction Sites Please inquire     
ACCN NM_173076
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation There are 2 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the conservative substitution of 1 amino acid (Thr/Ser at amino acid 777).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173076.2, NP_775099.2
RefSeq Size 9112 bp
RefSeq ORF 7788 bp
Locus ID 26154
UniProt ID Q86UK0
Protein Families Druggable Genome, Transmembrane
Protein Pathways ABC transporters
Gene Summary The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, and White). This encoded protein is a member of the ABC1 subfamily, which is the only major ABC subfamily found exclusively in multicellular eukaryotes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes the longer isoform (a).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...