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G0 Protein alpha (GNAO1) (NM_138736) Human Untagged Clone
CAT＃: SC306072
GNAO1 (untagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 2
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEE17; EIEE17; G-ALPHA-o; GNAO; HLA-DQB1; NEDIM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NCBI ORF sequence for NM_138736, the custom clone sequence may differ by one or more nucleotides
ATGGGATGTACTCTGAGCGCAGAGGAGAGAGCCGCCCTCGAGCGGAGCAAGGCGATTGAG AAAAACCTCAAAGAGGATGGCATCAGCGCCGCCAAAGACGTGAAATTACTCCTGCTCGGG GCTGGAGAATCAGGAAAAAGCACCATTGTGAAGCAGATGAAGATCATCCATGAAGATGGC TTCTCCGGAGAAGACGTGAAACAGTACAAGCCTGTTGTCTACAGCAACACTATCCAGTCC CTGGCAGCCATCGTCCGGGCCATGGACACTTTGGGCATCGAATATGGTGATAAGGAGAGA AAGGCTGACGCCAAGATGGTGTGTGATGTGGTGAGTCGGATGGAAGACACCGAGCCCTTC TCTGCAGAGCTGCTTTCTGCCATGATGCGGCTCTGGGGCGACTCAGGAATCCAAGAGTGC TTCAACCGGTCCCGGGAGTATCAGCTCAACGACTCTGCCAAATACTACCTGGACAGCCTG GATCGGATTGGGGCCGCCGACTACCAGCCCACCGAGCAGGACATCCTCCGAACCAGGGTC AAAACCACTGGCATCGTAGAAACCCACTTCACATTCAAGAACCTCCACTTCAGGCTGTTT GACGTCGGAGGCCAGCGATCTGAACGCAAGAAGTGGATCCATTGCTTCGAGGACGTCACG GCCATCATTTTCTGTGTCGCGCTCAGCGGCTATGACCAGGTGCTCCACGAAGACGAAACC ACGAACCGCATGCACGAATCCCTGAAGCTTTTTGACAGCATCTGCAACAACAAATGGTTC ACAGACACGTCCATCATCCTGTTTCTTAACAAGAAGGACATATTTGAAGAGAAGATCAAG AAGTCCCCGCTCACCATCTGCTTTCCTGAATATACAGGCCCCAGCGCCTTCACAGAAGCC GTGGCTTACATCCAGGCCCAGTACGAGAGCAAGAACAAGTCAGCCCACAAAGAGATCTAC ACCCACGTCACCTGCGCCACGGACACCAACAACATCCAGTTTGTCTTTGATGCTGTGACG GACGTCATCATCGCCAAAAACCTGCGGGGCTGTGGACTCTACTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_138736 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138736.1, NP_620073.1 |
| RefSeq Size | 1065 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 2775 |
| UniProt ID | P09471 |
| Protein Families | Druggable Genome |
| Protein Pathways | Long-term depression, Melanogenesis |
| Gene Summary | The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) is the same length but has a distinct C-terminus, compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215437 | GNAO1 (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 2 |
CNY 5,856.00 |
|
| RC215437L3 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215437L4 | Lenti ORF clone of Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG215437 | GNAO1 (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O (GNAO1), transcript variant 2 |
CNY 7,456.00 |
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