TLR4 (NM_138556) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARMD10; CD284; homolog of Drosophila toll; hToll; OTTHUMP00000022807; TOLL; TOLL, hToll; toll-like receptor 4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_138556 edited GGACTCTGATCCCAGCCATGGCCTTCCTCTCCTGCGTGAGACCAGAAAGCTGGGAGCCCT GCGTGGAGACTTGGCCCTAAACCACACAGAAGAGCTGGCATGAAACCCAGAGCTTTCAGA CTCCGGAGCCTCAGCCCTTCACCCCGATTCCATTGCTTCTTGCTAAATGCTGCCGTTTTA TCACGGAGGTGTGAAATCCAGACAATTGAAGATGGGGCATATCAGAGCCTAAGCCACCTC TCTACCTTAATATTGACAGGAAACCCCATCCAGAGTTTAGCCCTGGGAGCCTTTTCTGGA CTATCAAGTTTACAGAAGCTGGTGGCTGTGGAGACAAATCTAGCATCTCTAGAGAACTTC CCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCTCACAATCTTATCCAATCT TTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAGCACTTGGACCTTTCCAGC AACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTACATCAAATGCCCCTACTC AATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATCCAACCAGGTGCATTTAAA GAAATTAGGCTTCATAAGCTGACTTTAAGAAATAATTTTGATAGTTTAAATGTAATGAAA ACTTGTATTCAAGGTCTGGCTGGTTTAGAAGTCCATCGTTTGGTTCTGGGAGAATTTAGA AATGAAGGAAACTTGGAAAAGTTTGACAAATCTGCTCTAGAGGGCCTGTGCAATTTGACC ATTGAAGAATTCCGATTAGCATACTTAGACTACTACCTCGATGGTATTATTGACTTATTT AATTGTTTGACAAATGTTTCTTCATTTTCCCTGGTGAGTGTGACTATTGAAAGGGTAAAA GACTTTTCTTATAATTTCGGATGGCAACATTTAGAATTAGTTAACTGTAAATTTGGACAG TTTCCCACATTGAAACTCAAATCTCTCAAAAGGCTTACTTTCACTTCCAACAAAGGTGGG AATGCTTTTTCAGAAGTTGATCTACCAAGCCTTGAGTTTCTAGATCTCAGTAGAAATGGC TTGAGTTTCAAAGGTTGCTGTTCTCAAAGTGATTTTGGGACAATCAGCCTAAAGTATTTA GATCTGAGCTTCAATGGTGTTATTACCATGAGTTCAAACTTCTTGGGCTTAGAACAACTA GAACATCTGGATTTCCAGCATTCCAATTTGAAACAAATGAGTGAGTTTTCAGTATTCCTA TCACTCAGAAACCTCATTTACCTTGACATTTCTCATACTCACACCAGAGTTGCTTTCAAT GGCATCTTCAATGGCTTGTCCAGTCTCGAAGTCTTGAAAATGGCTGGCAATTCTTTCCAG GAAAACTTCCTTCCAGATATCTTCACAGAGCTGAGAAACTTGACCTTCCTGGACCTCTCT CAGTGTCAACTGGAGCAGTTGTCTCCAACAGCATTTAACTCACTCTCCAGTCTTCAGGTA CTAAATATGAGCCACAACAACTTCTTTTCATTGGATACGTTTCCTTATAAGTGTCTGAAC TCCCTCCAGGTTCTTGATTACAGTCTCAATCACATAATGACTTCCAAAAAACAGGAACTA CAGCATTTTCCAAGTAGTCTAGCTTTCTTAAATCTTACTCAGAATGACTTTGCTTGTACT TGTGAACACCAGAGTTTCCTGCAATGGATCAAGGACCAGAGGCAGCTCTTGGTGGAAGTT GAACGAATGGAATGTGCAACACCTTCAGATAAGCAGGGCATGCCTGTGCTGAGTTTGAAT ATCACCTGTCAGATGAATAAGACCATCATTGGTGTGTCGGTCCTCAGTGTGCTTGTAGTA TCTGTTGTAGCAGTTCTGGTCTATAAGTTCTATTTTCACCTGATGCTTCTTGCTGGCTGC ATAAAGTATGGTAGAGGTGAAAACATCTATGATGCCTTTGTTATCTACTCAAGCCAGGAT GAGGACTGGGTAAGGAATGAGCTAGTAAAGAATTTAGAAGAAGGGGTGCCTCCATTTCAG CTCTGCCTTCACTACAGAGACTTTATTCCCGGTGTGGCCATTGCTGCCAACATCATCCAT GAAGGTTTCCATAAAAGCCGAAAGGTGATTGTTGCGGTGTCCCAGCACTTCATCCAGAGC CGCTGGTGTATCTTTGAATATGAGATTGCTCAGACCTGGCAGTTTCTGAGCAGTCGTGCT GGTATCATCTTCATTGTCCTGCAGAAGGTGGAGAAGACCCTGCTCAGGCAGCAGGTGGAG CTGTACCGCCTTCTCAGCAGGAACACTTACCTGGAGTGGGAGGACAGTGTCCTGGGGCGG CACATCTTCTGGAGACGACTCAGAAAAGCCCTGCTGGATGGTAAATCATGGAATCCAGAA GGAACAGTGGGTACAGGATGCAATTGGCAGGAAGCAACATCTATCTGAAGAGGAAAAATA AAAACCTCCTGAGGCATTTCTTGCCCAGCTGGGTCCAACACTTGTTCAGTTAATAAGTAT TAAATGCTGCCACATGTCAGGCCTTATG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_138556 unedited GGGGGGTTAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCGAGC CACGCATTCACAGGGCCACTGCTGCTCACAGAAGCAGTGAGGATGATGCCAGGATGATGT CTGCCTCGCGCCTGGCTGGGACTCTGATCCCAGCCATGGCCTTCCTCTCCTGCGTGAGAC CAGAAAGCTGGGAGCCCTGCGTGGAGACTTGGCCCTAAACCACACAGAAGAGCTGGCATG AAACCCAGAGCTTTCAGACTCCGGAGCCTCAGCCCTTCACCCCGATTCCATTGCTTCTTG CTAAATGCTGCCGTTTTATCACGGAGGTGTGAAATCCAGACAATTGAAGATGGGGCATAT CAGAGCCTAAGCCACCTCTCTACCTTAATATTGACAGGAAACCCCATCCAGAGTTTAGCC CTGGGAGCCTTTTCTGGACTATCAAGTTTACAGAAGCTGGTGGCTGTGGAGACAAATCTA GCATCTCTAGAGAACTTCCCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCT CACAATCTTATCCAATCTTTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAG CACTTGGACCTTTCCAGCAACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTA CATCAAATGCCCCTACTCAATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATC CAACCAGGTGCATTTAAAGAAATTANGCTTCATAAGCTGACTTTAAGAAATAATTTTGAT AGTTTAAATGTAATGAANACTTGTATTCANAGTCTGGCTGGTTTAGAAGTCCATCGTTTG GTTCTGGGAGAATTTAGAAATGAANGAAACTTGGNAAAGTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' genomic read for NM_138556 unedited NTTCTCATCGCACACGTTACAGAAGCTGGTGGCTGTGGAGACAATCTAGCATCTCTATAG AACTTCGCCATTGGACATCTCAAAACTTTGAAAGAACTTAATGTGGCTCACAATCTTATC CAATCTTTCAAATTACCTGAGTATTTTTCTAATCTGACCAATCTAGAGCACTTGGACCTT TCCAGCAACAAGATTCAAAGTATTTATTGCACAGACTTGCGGGTTCTACATCAAATGCCC CTACTCAATCTCTCTTTAGACCTGTCCCTGAACCCTATGAACTTTATCCAACCAGGTGCA TTTAAAGAAATTAGGCTTCATAAGCTGACTTTAAGAAATAATTTTGATAGTTTAACCGTA ATGAAAACTTGTATTCAAGGTCTGGCTGGTTTAGAAGTCCATCGTTTGGTTCTGGGAGAA TTTAGAAATGAAGGAAACTTGGAAAAGTTTGACAAATCTGCTCTAGAGGGCCTGTGCAAT TTGACCATTGAAGAATTCCGATTAGCATACTTAGACTACTACCTCGATGGTATTATTGAC TTATTTAATTGTTTGACAAATGTTTCTTCATTTTCCCTGGTGAGTGTGACTATTGAAAGG GTAAAAGACTTTTCTTATAATTTCGGATGGCAACATTTACAATTAGTTAACTGTAAATTT GGACAGTTTCCCACATTGAAACTCAAATCTCTCAAAAGGCTTACTTTCACTTCCAACAAA GGTGGGAATGCTTTTTCAGAAGTTGATCTACCAAGCCTTGAGTTTCTAGATCTCAGTACA AATGGCTTGAGTTTCAAAGGTTGCTGTTCTCAAAGTGATTTTGGGACAATCAGCCTAAAG TATTTAGATCTGAGCTTCAATGGTGTTATTACCATGAGTTCAAACTTCTTGGGCTTAGAA CAACTAGAACATCTTAATTTCCAGCATTCCAATTTGAACAAATGAGTGAGTTTTCAGTAT TCCTATCACTCAAAACCTCATTTACCTTGACATTTCTCTACTCACACCAGAATTGCTTTC ATGGCATCTTCAATGGCTGTCCATCCCCAAGTCTGGAAATGGCTTGGCAATTCTTCCCGG AAAACTTCCTTTCCAATTCCTTCCCGGAGCGGAAAAACTTGACCTTCCTGGACCCCTCCC AAGGTCAATGGGAACAACTGGTCCCCCACGGCTTTTAATTCACCCCTCAGTCTTTCGGGT TATAAGATATGAGACCCCAGAACCTTCTCTTTTTTGGTGAAACGCTTCCCTCCTTAAGGT GAGTTAATAATCTCTCCTCGGAGTGGTTTGTAATGATGCCCTCGAGCGCCTCAAACGCCG CTCTCAAACATATAAGGGATAGATNTTATGTTTTTCTNGTCGTGCTCTAACCTCTCTNTC ACATTCGATGATGAAGAGAAT
Restriction Sites	Please inquire
ACCN	NM_138556
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	There are 3 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 3 amino acids.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138556.1, NP_612566.1
RefSeq Size	3767 bp
RefSeq ORF	2349 bp
Locus ID	7099
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway
Gene Summary	The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2020] Transcript Variant: This variant (2) uses an alternative exon 2 compared to transcript variant 1, and encodes an isoform (B) with a different N-terminus compared to that of isoform (A).

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