TLR4 Mouse Monoclonal Antibody [Clone ID: 76B357.1]

Specifications

Product Data
Clone Name	76B357.1
Applications	Block/Neutralize, ChIP, CyTOF-ready, Dot, ELISA, FC, ICC/IF, IHC, SDS-PAGE, WB
Recommend Dilution	SDS-Page, Knockdown Validated, Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, ELISA, Block/Neutralize, Knockout Validated, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, In vitro assay, ELISA Capture (Matched Antibody Pair): 0.5 ug, Western Blot: 1-3 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 5 ug/ml, Immunohistochemistry-Frozen: 1:500, CyTOF-ready, Dual RNAscope ISH-IHC
Reactivity	Human, Mouse, Rat, Squirrel
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was developed against a portion of amino acids 100-200 of human TLR4 (NP_612564).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	toll like receptor 4
Database Link	NP_612564 Entrez Gene 21898 MouseEntrez Gene 29260 RatEntrez Gene 7099 Human UniProt ID O00206
Background	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa (6).
Synonyms	ARMD10; CD284; TLR-4; TOLL
Note	Flow (Intracellular) use reported in literature (See Cohen et al, 2008); Immunofluorescence (See Nowicki et al, 2009); IHC-F (Nowicki et al, 2012); Flow (cell surface) use reported in scientific literature (PMID 23796194)
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

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