MADCAM1 (NM_130761) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MACAM1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF sequence for NM_130761 edited GGCGGCCGCGAATTCATGGATTTCGGACTGGCCCTCCTGCTGGCGGGGCTTCTGGGGCTC CTCCTCGGCCAGTCCCTCCAGGTGAAGCCCCTGCAGGTGGAGCCCCCGGAGCCGGTGGTG GCCGTGGCCTTGGGCGCCTCGCGCCAGCTCACCTGCCGCCTGGCCTGCGCGGACCGCGGG GCCTCGGTGCAGTGGCGGGGCCTGGACACCAGCCTGGGCGCGGTGCAGTCGGACACGGGC CGCAGCGTCCTCACCGTGCGCAACGCCTCGCTGTCGGCGGCCGGGACCCGCGTGTGCGTG GGCTCCTGCGGGGGCCGCACCTTCCAGCACACCGTGCAGCTCCTTGTGTACGCCTTCCCG GACCAGCTGACCGTCTCCCCRGCAGCCCTGGTGCCTGGTGACCCGGAGGTGGCCTGTACG GCCCACAAAGTCACGCCCGTGGACCCCAACGCGCTCTCCTTCTCCCTGCTCGTCGGGGGC CAGGAACTGGAGGGGGCGCAAGCCCTGGGCCCGGAGGTGCAGGAGGAGGAGGAGGAGCCC CAGGGGGACGAGGACGTGCTGTTCAGGGTGACAGAGCGCTGGCGGCTGCCGCCCCTGGGG ACCCCTGTCCCGCCCGCCCTCTACTGCCAGGCCACGATGAGGCTGCCTGGCTTGGAGCTC AGCCACCGCCAGGCCATCCCCGTCCTGCACAGCCCGACCTCCCCGGAGCCTCCCAACACC ACCTCCCCGGAGCCTCCCAACACCACCTCCCCGGAGTCTCCCGACACCACCTCCCCGGAG TCTCCCGACACCACCTCCCAGGAGCCTCCCGACACCACCTCCCAGGAGCCTCCCGACACC ACCTCCCAGGAGCCTCCCGACACCACCTCCCCGGAGCCTCCCGACAAGACCTCCCCGGAG CCCGCCCCCCAGCAGGGCTCCACACACACCCCCAGGAGCCCAGGCTCCACCAGGACTCGC CGCCCTGAGATCTCCCAGGCTGGGCCCACGCAGGGAGAAGTGATCCCAACAGGCTCGTCC AAACCTGCGGGTGACCAGCTGCCCGCGGCTCTGTGGACCAGCAGTGCGGTGCTGGGACTG CTGCTCCTGGCCTTGCCCACCTATCACCTCTGGAAACGCTGCCGGCACCTGGCTGAGGAC GACACCCACCCACCGGCTTCTCTGAGGCTTCTGCCCCAGGTGTCGGCCTGGGCTGGGTTA AGGGGGACCGGCCAGGTCGGGATCAGCCCCTCCTGAGTGGCCAGCCTTTCCCCCTGTGAA AGCAAAATAGCTTGGACCCCTTCAAGTTGAGAACTGGTCAGGGCAAACCTGCCTCCCATT CTACTCAAAGTCATCCCTCTGCTCACAGAGATGGATGCATGTTCTGATTGCCTCTTTGGA GAAGCTCATCAGAAACTCAAAAGAAGGCCACTGTTTGTCTCACCTACCCATGACCTGAAG CCCCTCCCTGAGTGGTCCCCACCTTTCTGGACGGAACCACGTACTTTTTACATACATTGA TTCATGTCTCACGTCTCCCTAAAAATGCGTAAGACCAAGCTGTGCCCTGACCACCCTGGG CCCCTGTCGTCAGGACCTCCTGAGGCTTTGGCAAATAAACCTCCTAAAATGATAAAAAAA AAAAAAAAAAAACT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_130761 unedited NAAGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGATTCTGGNTTTCGGACTGGCC CTCCTGCTGGCGGGGCTTCTGGGGCTCCTCCTCGGCCAGTCCCTCCAGGTGAAGCCCCTG CAGGTGGAGCCCCCGGAGCCGGTGGTGGCCGTGGCCTTGGGCGCCTCGCGCCAGCTCACC TGCCGCCTGGCCTGCGCGGACCGCGGGGCCTCGGTGCAGTGGCGGGGCCTGGACACCAGC CTGGGCGCGGTGCAGTCGGACACGGGCCGCAGCGTCCTCACCGTGCGCAACGCCTCGCTG TCGGCGGCCGGGACCCGCGTGTGCGTGGGCTCCTGCGGGGGCCGCACCTTCCAGCACACC GTGCAGCTCCTTGTGTACGCCTTCCCGGACCAGCTGACCGTCTCCCCGGCAGCCCTGGTG CCTGGTGACCCGGAGGTGGCCTGTACGGCCCACAAAGTCACGCCCGTGGACCCCAACGCG CTCTCCTTCTCCCTGCTCGTCGGGGGCCAGGAACTGGAGGGGGCGCAAGCCCTGNGCCCG GAGGTGCAGGAGGAGGAGGAGGAGCCCCAGGGGGACGAGGACGTGCTGTTCAGGGTGACA GAGCGCTGGCGGCTGCCGCCCCTGGGGACCCCTGTCCCGCCCGCCCTCTACTGCCAGGCC ACGATGAGGCTGCCTGGCTTGGAGCTCAGCCACCGCCAGGCCATCCCCGTCCTGCACAGC CCGACCTCCCCGGAGCCTCCCAACACCACCTCCCCGGAGCCTCCCAACACCACCTCCCCG GAGTCTCCCGACACCACCCTCCCGGGATCTCCCGACACCACCTCCCAGGACCCTCCCGAC CCACCTCCAAGGAGCCTCCCGACACAACTCCCAGGAACCTCCCGAAACAACTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_130761 unedited CTANGATCGAGTTTTTTTTTTTTTTTTTTTATCATTTTAGGAGGTTTATTTGCCAAAGCC TCAGGAGGTCCTGACGACAGGGGCCCAGGGTGGTCAGGGCACAGCTTGGTCTTACGCATT TTTAGGGAGACGTGAGACATGAATCAATGTATGTAAAAAGTACGTGGTTCCGTCCAGAAA GGTGGGGACCACTCAGGGAGGGGCTTCAGGTCATGGGTAGGTGAGACAAACAGTGGCCTT CTTTTGAGTTTCTGATGAGCTTCTCCAAAGAGGCAATCAGAACATGCATCCATCTCTGTG AGCAGAGGGATGACTTTGAGTAGAATGGGAGGCAGGTTTGCCCTGACCAGTTCTCAACTT GAAGGGGTCCAAGCTATTTTGCTTTCACAGGGGGAAAGGCTGGCCACTCAGGAGGGGCTG ATCCCGACCTGGCCGGTCCCCCTTAACCCAGCCCAGGCCGACACCTGGGGCAGAAGCCTC AGAGAAGCCGGTGGGTGGGTGTCGTCCTCAGCCAGGTGCCGGCAGCGTTTCCAGAGGTGA TAGGTGGGCAAGGCCAGGAGCAGCAGTCCCAGCACCGCACTGCTGGTCCACAGAGCCGCG GGCAGCTGGTCACCCGCAGGTTTGGACGAGCCTGTTGGGATCACTTCTCCCTGCGTGGGC CCAGCCTGGGAGATCTCAGGGCGGCGAGTCCTGGTGGAGCCTGNGCTCCTGGGGGTGTGG TGTTGGAGCCCTGCTGGGGGGGCGGCTCCGGGGAGGTCTTGTCGGGAGGCTCCCGGGAGG TGGTGTCCGGNGCCTCCTGGAGGTGGTGTCCCGNGGCCTCCGGGAGTGGTGCCCGNGGCC TCCGGGGGTGGCGCCGGGAAACCCGGGGAGGGGTTG
Restriction Sites	Please inquire
ACCN	NM_130761
Insert Size	1700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_130761.1, NP_570117.1
RefSeq Size	1608 bp
RefSeq ORF	1608 bp
Locus ID	8174
Protein Pathways	Cell adhesion molecules (CAMs)
Gene Summary	The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the longest, predominant transcript and encodes the full-length isoform (a).

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