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GPR124 (ADGRA2) (NM_032777) Human Untagged Clone
CAT＃: SC305501
GPR124 (untagged)-Human G protein-coupled receptor 124 (GPR124)
CNY 25,650.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GPR124; TEM5 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_032777, the custom clone sequence may differ by one or more nucleotides
ATGGGCGCCGGGGGACGCAGGATGCGGGGGGCGCCCGCGCGCCTGCTGCTGCCGCTGCTG CCGTGGCTCCTGCTGCTCCTGGCGCCCGAGGCTCGGGGCGCGCCCGGCTGCCCGCTATCC ATCCGCAGCTGCAAGTGCTCGGGGGAGCGGCCCAAGGGGCTGAGCGGCGGCGTCCCTGGC CCGGCTCGGCGGAGGGTGGTGTGCAGCGGCGGGGACCTCCCGGAGCCTCCCGAGCCCGGC CTTCTGCCTAACGGCACCGTTACCCTGCTCTTGAGCAATAACAAGATCACGGGGCTCCGC AATGGCTCCTTCCTGGGACTGTCACTGCTGGAGAAGCTGGACCTGAGGAACAACATCATC AGCACAGTGCAGCCGGGCGCCTTCCTGGGCCTGGGGGAGCTGAAGCGTTTAGATCTCTCC AACAACCGGATTGGCTGTCTCACCTCCGAGACCTTCCAGGGCCTCCCCAGGCTTCTCCGA CTAAACATATCTGGAAACATCTTCTCCAGTCTGCAACCTGGGGTCTTTGATGAGCTGCCA GCCCTTAAGGTTGTGGACTTGGGCACCGAGTTCCTGACCTGTGACTGCCACCTGCGCTGG CTGCTGCCCTGGGCCCAGAATCGCTCCCTGCAGCTGTCGGAACACACGCTCTGTGCTTAC CCCAGTGCCCTGCATGCTCAGGCCCTGGGCAGCCTCCAGGAGGCCCAGCTCTGCTGCGAG GGGGCCCTGGAGCTGCACACACACCACCTCATCCCGTCCCTACGCCAAGTGGTGTTCCAG GGGGATCGGCTGCCCTTCCAGTGCTCTGCCAGCTACCTGGGCAACGACACCCGCATCCGC TGGTACCACAACCGAGCCCCTGTGGAGGGTGATGAGCAGGCGGGCATCCTCCTGGCCGAG AGCCTCATCCACGACTGCACCTTCATCACCAGTGAGCTGACGCTGTCTCACATCGGCGTG TGGGCCTCAGGCGAGTGGGAGTGCACCGTGTCCATGGCCCAAGGCAACGCCAGCAAGAAG GTGGAGATCGTGGTGCTGGAGACCTCTGCCTCCTACTGCCCCGCCGAGCGTGTTGCCAAC AACCGCGGGGACTTCAGGTGGCCCCGAACTCTGGCTGGCATCACAGCCTACCAGTCCTGC CTGCAGTATCCCTTCACCTCAGTGCCCCTGGGCGGGGGTGCCCCGGGCACCCGAGCCTCC CGCCGGTGTGACCGTGCCGGCCGCTGGGAGCCAGGGGACTACTCCCACTGTCTCTACACC AACGACATCACCAGGGTGCTGTACACCTTCGTGCTGATGCCCATCAATGCCTCCAATGCG CTGACCCTGGCTCACCAGCTGCGCGTGTACACAGCCGAGGCCGCTAGCTTTTCAGACATG ATGGATGTAGTCTATGTGGCTCAGATGATCCAGAAATTTTTGGGTTATGTCGACCAGATC AAAGAGCTGGTAGAGGTGATGGTGGACATGGCCAGCAACCTGATGCTGGTGGACGAGCAC CTGCTGTGGCTGGCCCAGCGCGAGGACAAGGCCTGCAGCCGCATCGTGGGTGCCCTGGAG CGCATTGGGGGGGCCGCCCTCAGCCCCCATGCCCAGCACATCTCAGTGAATGCGAGGAAC GTGGCATTGGAGGCCTACCTCATCAAGCCGCACAGCTACGTGGGCCTGACCTGCACAGCC TTCCAGAGGAGGGAGGGAGGGGTGCCGGGCACACGGCCAGGAAGCCCTGGCCAGAACCCC CCACCTGAGCCCGAGCCCCCAGCTGACCAGCAGCTCCGCTTCCGCTGCACCACCGGGAGG CCCAATGTTTCTCTGTCGTCCTTCCACATCAAGAACAGCGTGGCCCTGGCCTCCATCCAG CTGCCCCCGAGTCTATTCTCATCCCTTCCGGCTGCCCTGGCTCCCCCGGTGCCCCCAGAC TGCACCCTGCAACTGCTCGTCTTCCGAAATGGCCGCCTCTTCCACAGCCACAGCAACACC TCCCGCCCTGGAGCTGCTGGGCCTGGCAAGAGGCGTGGCGTGGCCACCCCCGTCATCTTC GCAGGAACCAGTGGCTGTGGCGTGGGAAACCTGACAGAGCCAGTGGCCGTTTCGCTGCGG CACTGGGCTGAGGGAGCCGAACCTGTGGCCGCTTGGTGGAGCCAGGAGGGGCCCGGGGAG GCTGGGGGCTGGACCTCGGAGGGCTGCCAGCTCCGCTCCAGCCAGCCCAATGTCAGCGCC CTGCACTGCCAGCACTTGGGCAATGTGGCCGTGCTCATGGAGCTGAGCGCCTTTCCCAGG GAGGTGGGGGGCGCCGGGGCAGGGCTGCACCCCGTGGTATACCCCTGCACGGCCTTGCTG CTGCTCTGCCTCTTCGCCACCATCATCACCTACATCCTCAACCACAGCTCCATCCGTGTG TCCCGGAAAGGCTGGCACATGCTGCTGAACTTGTGCTTCCACATAGCCATGACCTCTGCT GTCTTTGCGGGGGGCATCACACTCACCAACTACCAGATGGTCTGCCAGGCGGTGGGCATC ACCCTGCACTACTCCTCCCTATCCACGCTGCTCTGGATGGGCGTGAAGGCGCGAGTGCTC CATAAGGAGCTCACCTGGAGGGCACCCCCTCCGCAAGAAGGGGACCCCGCTCTGCCTACT CCCAGTCCTATGCTCCGGTTCTATTTGATCGCTGGAGGGATTCCACTCATTATCTGTGGC ATCACAGCTGCAGTCAACATCCACAACTACCGGGACCACAGCCCCTACTGCTGGCTGGTG TGGCGTCCAAGCCTTGGCGCCTTCTACATCCCTGTGGCTTTGATTCTGCTCATCACCTGG ATCTATTTCCTGTGCGCCGGGCTACGCTTACGGGGTCCTCTGGCACAGAACCCCAAGGCG GGCAACAGCAGGGCCTCCCTGGAGGCAGGGGAGGAGCTGAGGGGTTCCACCAGGCTCAGG GGCAGCGGCCCCCTCCTGAGTGACTCAGGTTCCCTTCTTGCTACTGGGAGCGCGCGAGTG GGGACGCCCGGGCCCCCGGAGGATGGTGACAGCCTCTATTCTCCGGGAGTCCAGCTAGGG GCGCTGGTGACCACGCACTTCCTGTACTTGGCCATGTGGGCCTGCGGGGCTCTGGCAGTG TCCCAGCGCTGGCTGCCCCGGGTGGTGTGCAGCTGCTTGTACGGGGTGGCAGCCTCCGCC CTGGGCCTCTTCGTCTTCACTCACCACTGTGCCAGGCGGAGGGACGTGAGAGCCTCGTGG CGCGCCTGCTGCCCCCCTGCCTCTCCCGCGGCCCCCCATGCCCCGCCCCGGGCCCTGCCC GCCGCCGCAGAGGACGGTTCCCCGGTGTTCGGGGAGGGCCCCCCCTCCCTCAAGTCCTCC CCAAGCGGCAGCAGCGGCCATCCGCTGGCTCTGGGCCCCTGCAAGCTCACCAACCTGCAG CTGGCCCAGAGTCAGGTGTGCGAGGCGGGGGCGGCGGCCGGCGGGGAAGGAGAGCCGGAG CCGGCGGGCACCCGGGGAAACCTCGCCCACCGCCACCCCAACAACGTGCACCACGGGCGT CGGGCGCACAAGAGCCGGGCCAAGGGACACCGCGCGGGGGAGGCCTGCGGCAAGAACCGG CTCAAGGCCCTGCGCGGGGGCGCGGCGGGGGCGCTGGAGCTGCTGTCCAGCGAGAGCGGC AGTCTGCACAACAGCCCCACCGACAGCTACCTGGGCAGCAGCCGCAACAGCCCGGGCGCC GGCCTGCAGCTGGAAGGCGAGCCCATGCTCACGCCGTCCGAGGGCAGCGACACCAGCGCC GCGCCGCTTTCTGAGGCGGGCCGGGCAGGCCAGCGCCGCAGCGCCAGCCGCGACAGTCTC AAGGGCGGCGGCGCGCTGGAGAAGGAGAGCCATCGCCGCTCGTACCCGCTCAACGCCGCC AGCCTAAACGGCGCCCCCAAGGGGGGCAAGTACGACGACGTCACCCTGATGGGCGCGGAG GTAGCCAGCGGCGGCTGCATGAAGACCGGACTCTGGAAGAGCGAAACTACCGTCTAA |
| Restriction Sites | Please inquire |
| ACCN | NM_032777 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_032777.6, NP_116166.6 |
| RefSeq Size | 5680 bp |
| RefSeq ORF | 3996 bp |
| Locus ID | 25960 |
| UniProt ID | Q96PE1 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (PubMed:30026314). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (PubMed:16982628).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC229431 | GPR124 (Myc-DDK-tagged)-Human G protein-coupled receptor 124 (GPR124) |
CNY 13,728.00 |
|
| RC229431L3 | Lenti ORF clone of Human G protein-coupled receptor 124 (GPR124), Myc-DDK-tagged |
CNY 15,200.00 |
|
| RC229431L4 | Lenti ORF clone of Human G protein-coupled receptor 124 (GPR124), mGFP tagged |
CNY 16,128.00 |
|
| RG229431 | GPR124 (tGFP-tagged) - Human G protein-coupled receptor 124 (GPR124) |
CNY 15,328.00 |
|
| SC328066 | GPR124 (untagged)-Human G protein-coupled receptor 124 (GPR124) |
CNY 13,744.00 |
