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PREX2 (NM_024870) Human Untagged Clone
CAT＃: SC305174
PREX2 (untagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1
CNY 30,880.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DEP.2; DEPDC2; P-REX2; PPP1R129 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305174 representing NM_024870.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCGAGGACAGCCGCGGAGACAGCCGCGCCGAGAGCGCCAAGGACCTGGAGAAGCAGCTTCGCCTG CGCGTGTGCGTGCTCAGCGAGCTCCAGAAGACCGAGCGGGACTATGTGGGCACGCTGGAGTTCCTGGTG TCGGCATTCTTACACAGAATGAACCAGTGTGCAGCATCAAAAGTTGACAAAAATGTGACAGAAGAAACA GTGAAGATGTTGTTCTCAAACATTGAAGACATCCTTGCAGTACATAAAGAATTCTTAAAAGTCGTGGAA GAATGCTTACACCCCGAACCTAATGCTCAACAAGAAGTGGGAACCTGCTTTCTTCACTTTAAAGACAAG TTTCGTATCTATGATGAATATTGTAGTAACCATGAGAAGGCACAAAAATTACTTCTTGAACTCAACAAA ATAAGAACAATCCGGACATTTCTTTTGAACTGCATGCTGCTTGGAGGACGGAAGAACACAGATGTTCCC TTGGAAGGATATTTAGTAACACCAATACAAAGAATATGCAAGTACCCTCTTATTTTGAAGGAGTTGCTG AAGCGGACTCCACGGAAACACAGTGACTATGCAGCAGTGATGGAAGCCCTCCAAGCCATGAAAGCTGTC TGTTCCAACATAAACGAGGCCAAGAGACAGATGGAGAAGTTAGAAGTTTTAGAGGAATGGCAGTCTCAC ATTGAAGGCTGGGAGGGGTCCAACATCACTGACACCTGCACTGAAATGCTAATGTGTGGAGTCTTACTG AAAATTTCTTCTGGAAATATTCAAGAACGGGTGTTTTTTCTTTTCGATAATCTTTTGGTGTACTGCAAA AGAAAACACAGACGGTTGAAGAACAGCAAGGCATCTACAGATGGACATCGGTACCTTTTTCGTGGCCGG ATCAACACGGAGGTGATGGAAGTGGAGAATGTGGATGATGGCACCGCTGATTTCCATAGCAGTGGACAC ATTGTTGTTAATGGATGGAAGATACATAACACAGCAAAAAATAAATGGTTTGTTTGTATGGCAAAAACA CCTGAAGAGAAGCATGAATGGTTTGAAGCTATTTTGAAAGAAAGAGAACGGCGGAAAGGTTTAAAATTA GGAATGGAGCAAGATACCTGGGTCATGATCTCTGAACAGGGTGAGAAACTTTATAAAATGATGTGCAGA CAAGGAAATCTGATCAAAGACCGAAAGAGAAAACTGACTACGTTCCCTAAATGCTTTCTTGGAAGCGAA TTTGTGTCATGGCTGTTGGAAATTGGAGAGATTCACAGGCCTGAGGAAGGCGTGCACTTGGGACAAGCA TTATTAGAAAATGGAATCATTCACCATGTTACTGATAAACATCAATTCAAACCAGAACAGATGTTATAT AGATTTCGCTATGATGATGGAACATTTTATCCAAGAAATGAGATGCAGGACGTGATTTCAAAGGGTGTA AGATTATATTGTCGTCTTCATAGCCTTTTTACTCCAGTGATAAGAGATAAAGATTACCATTTAAGGACC TACAAATCTGTGGTCATGGCCAACAAACTGATAGACTGGTTAATTGCACAGGGAGATTGCCGCACCAGA GAAGAGGCAATGATATTTGGCGTTGGACTCTGTGACAATGGATTTATGCACCATGTCCTTGAAAAAAGC GAATTCAAAGATGAACCCCTACTTTTCCGTTTTTTTTCGGATGAGGAAATGGAGGGATCAAATATGAAA CATCGACTTATGAAACATGACTTAAAAGTTGTGGAAAATGTTATAGCTAAGTCATTATTGATTAAATCC AATGAAGGCAGCTATGGCTTTGGATTAGAAGACAAAAATAAAGTTCCAATAATAAAGTTGGTAGAAAAG GGATCTAATGCTGAGATGGCTGGCATGGAAGTCGGGAAAAAGATTTTTGCTATTAATGGTGACCTAGTT TTTATGAGACCTTTCAATGAAGTGGATTGCTTCCTGAAATCGTGTTTAAACAGCAGAAAACCTCTAAGA GTTCTTGTGAGCACAAAGCCAAGAGAGACAGTGAAAATTCCAGATTCAGCTGATGGACTTGGCTTCCAG ATCCGGGGATTTGGCCCTTCTGTTGTGCATGCTGTAGGAAGAGGAACTGTGGCTGCAGCAGCTGGTCTT CACCCTGGACAGTGCATTATCAAGGTGAATGGAATCAATGTCAGCAAAGAGACACATGCCAGTGTCATT GCACACGTTACAGCCTGCAGGAAGTACAGGCGGCCAACGAAGCAAGATTCCATACAATGGGTTTATAAT AGCATTGAGAGTGCTCAAGAAGACCTTCAAAAATCTCACTCCAAGCCCCCTGGAGATGAAGCAGGGGAT GCTTTTGACTGTAAAGTAGAAGAGGTTATTGACAAATTCAACACTATGGCCATCATTGATGGGAAGAAG GAGCATGTGAGTCTGACAGTGGACAATGTCCACCTGGAATATGGTGTCGTGTATGAGTACGACAGCACA GCTGGCATCAAGTGCAATGTGGTGGAAAAGATGATTGAGCCCAAAGGTTTCTTCAGCTTAACTGCCAAG ATTCTTGAAGCCCTGGCTAAAAGTGATGAGCATTTTGTACAAAACTGTACCAGCCTAAATTCTCTAAAT GAAGTGATTCCTACTGACCTTCAGAGTAAATTCAGTGCCCTTTGCAGTGAAAGAATTGAACACCTATGT CAGAGAATATCCAGTTATAAAAAGTTTTCTCGTGTACTGAAGAATAGGGCCTGGCCTACTTTTAAACAG GCCAAATCTAAAATCTCCCCACTGCACAGCAGTGATTTCTGCCCTACCAACTGCCATGTCAATGTGATG GAAGTTTCTTATCCCAAAACATCAACCTCTTTGGGAAGTGCATTTGGTGTTCAGTTGGATAGCAGGAAG CATAATTCTCATGATAAAGAAAACAAATCTTCGGAGCAAGGGAAACTGAGCCCTATGGTGTACATTCAG CACACCATCACAACCATGGCGGCCCCTTCAGGTCTGTCTCTGGGACAGCAGGATGGCCATGGTCTCAGG TATCTGCTAAAAGAAGAAGACTTAGAAACCCAAGACATCTATCAGAAACTGCTGGGCAAACTTCAGACT GCACTGAAAGAGGTGGAGATGTGTGTTTGTCAAATAGATGACCTTCTGTCTTCAATAACATATTCTCCT AAATTAGAACGTAAGACATCAGAGGGCATAATACCAACAGACAGTGACAATGAGAAGGGAGAAAGAAAC AGCAAACGGGTATGTTTTAATGTAGCAGGAGATGAACAGGAAGATTCTGGTCATGACACCATCAGCAAC AGAGACTCTTACAGTGACTGCAACAGCAATAGGAATTCCATCGCCTCCTTCACCAGCATCTGCAGCAGC CAGTGCAGCTCGTATTTCCACAGTGATGAAATGGACTCAGGTGATGAACTTCCCTTAAGTGTTCGCATA TCTCATGATAAACAGGACAAGATACATAGTTGCCTTGAGCATCTTTTCAGCCAGGTGGATTCAATTACC AATCTCCTAAAAGGGCAGGCTGTTGTGAGGGCCTTTGACCAAACCAAGTATCTCACTCCAGGCCGAGGA TTGCAAGAATTTCAACAGGAAATGGAACCAAAGCTGAGTTGTCCAAAAAGGCTACGGCTTCATATCAAG CAAGATCCTTGGAATCTTCCCAGCAGCGTCCGGACTCTTGCTCAGAACATCAGGAAATTTGTTGAAGAG GTAAAGTGTAGGCTACTCCTGGCTCTTCTTGAATATTCAGATAGTGAGACACAGCTCCGTAGAGACATG GTTTTCTGCCAGACTCTTGTGGCCACTGTCTGTGCCTTCTCTGAGCAGCTCATGGCGGCCTTGAACCAG ATGTTTGACAACAGCAAGGAAAATGAGATGGAAACTTGGGAAGCCAGCAGGAGGTGGCTGGACCAGATA GCGAATGCAGGTGTTCTTTTTCACTTTCAGTCACTTCTGTCACCAAACTTGACAGATGAACAAGCCATG TTAGAAGATACACTGGTTGCACTATTTGATTTGGAAAAGGTTTCCTTTTACTTTAAACCATCAGAAGAG GAACCTCTGGTTGCAAATGTTCCTCTTACATATCAAGCAGAAGGAAGTCGGCAAGCTCTGAAAGTTTAC TTCTACATTGATAGTTATCATTTTGAACAACTTCCTCAACGGCTGAAAAATGGAGGAGGGTTTAAAATT CATCCTGTTCTTTTTGCACAAGCACTAGAGAGCATGGAAGGATATTATTACAGAGACAATGTTTCTGTG GAAGAATTTCAAGCTCAGATAAATGCAGCCTCACTGGAAAAGGTCAAACAGTACAACCAGAAGCTCAGG GCATTCTACTTGGACAAGTCAAATTCACCACCAAACTCCACATCCAAAGCTGCCTATGTAGATAAGCTA ATGAGGCCTCTCAACGCTTTGGATGAACTTTACCGACTGGTAGCCTCGTTTATCAGATCCAAGCGCACA GCTGCCTGTGCAAACACAGCTTGCAGTGCTTCTGGGGTTGGACTGCTGTCAGTTTCCTCGGAGCTGTGC AACAGGCTGGGCGCCTGCCACATCATCATGTGCAGCAGCGGTGTGCATCGGTGCACCCTGAGCGTGACG CTGGAGCAAGCCATCATTCTGGCCAGAAGCCACGGACTGCCACCTCGTTACATCATGCAGGCTACAGAT GTGATGCGGAAGCAGGGAGCAAGAGTTCAGAACACAGCGAAGAATTTGGGAGTCAGAGACCGGACTCCA CAGTCTGCACCAAGGCTGTACAAGCTGTGCGAGCCACCTCCCCCAGCTGGAGAAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024870 |
| Insert Size | 4821 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024870.3 |
| RefSeq Size | 10768 bp |
| RefSeq ORF | 4821 bp |
| Locus ID | 80243 |
| UniProt ID | Q70Z35 |
| MW | 182.6 kDa |
| Gene Summary | The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223671 | PREX2 (Myc-DDK-tagged)-Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1 |
CNY 10,992.00 |
|
| RC223671L1 | Lenti ORF clone of Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1, Myc-DDK-tagged |
CNY 13,392.00 |
|
| RC223671L2 | Lenti ORF clone of Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1, mGFP tagged |
CNY 17,960.00 |
|
| RC223671L3 | Lenti ORF clone of Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1, Myc-DDK-tagged |
CNY 17,960.00 |
|
| RC223671L4 | Lenti ORF clone of Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1, mGFP tagged |
CNY 17,960.00 |
|
| RG223671 | PREX2 (tGFP-tagged) - Human phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 (PREX2), transcript variant 1 |
CNY 17,670.00 |
