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DDX4 (NM_024415) Human Untagged Clone
CAT＃: SC305113
DDX4 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | VASA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305113 representing NM_024415.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAG GATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGAT GGACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGAT GCTGGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGA AACAGAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAAT GACTGCGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGGCTATCGAGATGGAAATAAT TCAGAAGCTTCAGGGCCATACAGAAGAGGTGGAAGAGGTAGTTTCCGAGGTTGCCGTGGAGGATTTGGT CTAGGAAGTCCAAATAATGACTTAGACCCAGACGAATGTATGCAGCGCACTGGTGGCCTTTTTGGTTCT AGAAGACCAGTATTAAGTGGCACAGGTAATGGTGATACTTCTCAAAGCAGAAGTGGCAGTGGAAGTGAA CGAGGTGGTTACAAAGGTTTAAATGAAGAAGTAATAACAGGCTCTGGAAAGAATTCTTGGAAGTCAGAA GCAGAAGGAGGAGAAAGTAGTGATACTCAAGGACCAAAAGTGACCTACATACCCCCTCCTCCACCTGAG GATGAGGACTCCATCTTTGCACATTATCAGACAGGCATAAACTTCGACAAATACGACACTATTCTTGTG GAAGTGTCTGGACATGATGCACCACCAGCAATTCTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTG AATAACAACATTGCTAAAGCTGGTTATACTAAGCTTACTCCTGTGCAAAAATACAGTATTCCTATCATA CTTGCAGGACGAGATTTGATGGCTTGCGCTCAAACAGGGTCTGGGAAGACTGCGGCTTTTCTCCTACCA ATTTTGGCTCATATGATGCATGATGGAATAACTGCCAGTCGTTTTAAAGAGTTGCAGGAACCAGAGTGT ATTATTGTAGCACCAACTCGAGAATTGGTCAACCAGATTTATTTGGAAGCCAGAAAATTTTCTTTTGGG ACTTGTGTAAGAGCTGTTGTTATATATGGGGGAACCCAGCTGGGACATTCAATTCGACAAATAGTACAA GGCTGTAATATATTATGTGCTACTCCTGGAAGACTGATGGATATCATAGGCAAAGAAAAGATTGGTCTC AAACAGATCAAATACTTAGTTTTGGATGAAGCTGATCGCATGTTGGATATGGGTTTTGGTCCAGAAATG AAGAAGTTAATTTCTTGCCCAGGAATGCCATCAAAGGAACAGCGCCAAACCCTTATGTTCAGTGCAACT TTTCCAGAGGAAATTCAAAGGTTGGCTGCAGAGTTTTTAAAGTCAAATTATCTGTTTGTTGCTGTTGGA CAAGTGGGTGGAGCATGTAGAGATGTTCAGCAGACCGTTCTCCAAGTTGGCCAGTTCTCAAAAAGAGAA AAGCTCGTTGAAATTCTGCGAAACATAGGGGATGAAAGAACTATGGTCTTTGTTGAAACTAAGAAAAAA GCAGATTTTATTGCAACTTTTCTTTGTCAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAA CAGAGAGAGCGGGAGCAAGCTCTTGGAGATTTTCGCTTTGGAAAGTGCCCAGTTCTTGTTGCTACTTCA GTAGCTGCCAGAGGGCTGGATATTGAAAATGTGCAACATGTTATCAATTTTGATCTTCCTTCTACCATT GATGAATATGTTCATCGAATTGGGCGTACTGGTCGTTGTGGGAATACTGGCAGAGCAATTTCCTTTTTT GATCTTGAATCGGATAACCATTTAGCACAGCCTCTAGTAAAAGTATTGACAGATGCTCAACAGGATGTT CCTGCATGGTTGGAAGAAATTGCCTTTAGTACATACATTCCTGGCTTCAGTGGTAGTACAAGAGGAAAC GTGTTTGCATCAGTTGATACCAGAAAGGGCAAGAGCACTTTGAACACAGCTGGGTTTTCTTCTTCACAA GCTCCCAATCCAGTAGATGATGAGTCATGGGATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024415 |
| Insert Size | 2175 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024415.2 |
| RefSeq Size | 2880 bp |
| RefSeq ORF | 2175 bp |
| Locus ID | 54514 |
| UniProt ID | Q9NQI0 |
| MW | 79.3 kDa |
| Gene Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210628 | DDX4 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1 |
CNY 7,952.00 |
|
| RC210628L1 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1, Myc-DDK-tagged |
CNY 10,352.00 |
|
| RC210628L2 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210628L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210628L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG210628 | DDX4 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 1 |
CNY 9,552.00 |
