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ATP6V0A4 (NM_020632) Human Untagged Clone
CAT＃: SC304787
ATP6V0A4 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1
CNY 7,220.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A4; ATP6N1B; ATP6N2; DRTA3; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304787 representing NM_020632.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGTCTGTGTTTCGAAGCGAGGAGATGTGTTTGTCACAACTGTTTCTCCAGGTGGAAGCTGCATAT TGCTGTGTGGCTGAGCTCGGAGAGCTCGGATTGGTTCAGTTCAAAGATTTAAATATGAATGTGAACAGC TTTCAAAGGAAATTTGTGAATGAAGTCAGAAGGTGTGAATCACTGGAGAGAATCCTCCGTTTTCTGGAA GACGAGATGCAAAATGAGATTGTAGTTCAGTTGCTCGAGAAAAGCCCACTGACCCCGCTCCCACGGGAA ATGATTACCCTGGAGACTGTTCTAGAAAAACTGGAAGGAGAGTTACAGGAAGCCAACCAGAACCAGCAG GCCTTGAAACAAAGCTTCCTAGAACTGACAGAACTGAAATACCTCCTGAAGAAAACCCAAGACTTCTTT GAGACGGAAACCAATTTAGCTGATGATTTCTTTACTGAGGACACTTCTGGCCTCCTGGAGTTGAAAGCA GTGCCTGCATATATGACCGGAAAGTTGGGGTTCATAGCCGGTGTGATCAACAGGGAGAGGATGGCTTCC TTTGAGCGGTTACTGTGGCGAATCTGCCGAGGAAACGTGTACTTGAAGTTCAGTGAGATGGACGCCCCT CTGGAGGATCCTGTGACGAAAGAAGAAATTCAGAAGAACATATTCATCATATTTTACCAAGGAGAGCAG CTCAGGCAGAAAATCAAGAAGATCTGTGATGGGTTTCGAGCCACTGTCTACCCTTGCCCAGAGCCTGCG GTGGAGCGCAGAGAGATGTTGGAGAGCGTCAATGTGAGGCTGGAAGATTTAATCACCGTCATAACACAA ACAGAGTCTCACCGCCAGCGCCTGCTGCAGGAAGCCGCTGCCAACTGGCACTCCTGGCTCATCAAGGTG CAGAAGATGAAAGCTGTCTACCACATCCTGAACATGTGCAACATCGACGTCACCCAGCAGTGTGTCATC GCCGAGATCTGGTTCCCGGTGGCAGATGCCACACGTATCAAGAGGGCACTGGAGCAAGGCATGGAACTA AGTGGCTCCTCCATGGCCCCCATCATGACCACAGTGCAATCTAAAACAGCCCCTCCCACATTTAACAGG ACCAATAAATTCACAGCTGGCTTCCAGAATATTGTTGATGCCTATGGTGTCGGCAGCTACCGGGAGATA AACCCAGCCCCCTACACCATCATCACTTTCCCCTTCCTGTTCGCTGTGATGTTTGGAGACTGTGGTCAT GGAACCGTGATGCTCCTGGCTGCACTTTGGATGATTCTGAATGAGAGACGCTTGCTCTCCCAGAAGACA GACAATGAGATTTGGAACACCTTCTTCCACGGGCGCTATCTGATCCTACTTATGGGCATCTTCTCCATC TACACGGGTTTGATCTACAATGACTGCTTCTCCAAGTCCTTGAACATCTTTGGCTCTTCTTGGAGTGTC CAACCCATGTTCAGAAACGGCACATGGAATACTCATGTAATGGAGGAAAGTCTATATCTGCAGCTGGAC CCAGCCATACCAGGAGTGTATTTTGGAAATCCATACCCGTTTGGGATTGATCCGATTTGGAACTTGGCT TCAAACAAACTCACATTTCTGAACTCGTATAAAATGAAGATGTCGGTGATCCTGGGAATTGTCCAGATG GTTTTCGGTGTCATCCTCAGCCTTTTCAATCACATATACTTCAGAAGAACTCTCAACATCATTCTGCAA TTTATCCCTGAGATGATTTTTATCCTGTGTCTGTTTGGATACCTGGTTTTCATGATCATTTTCAAATGG TGCTGCTTTGACGTCCATGTATCTCAGCACGCCCCCAGCATCCTCATCCACTTCATCAACATGTTTCTG TTTAACTACAGTGACTCTTCCAACGCACCCCTCTACAAACATCAGCAAGAAGTCCAAAGTTTCTTTGTG GTTATGGCTTTGATTTCTGTGCCGTGGATGCTTCTGATTAAGCCGTTTATTCTTAGAGCCAGTCATCGG AAATCCCAGCTGCAGGCATCCAGGATCCAAGAAGATGCCACTGAGAACATTGAAGGTGATAGCTCCAGC CCTTCTAGCCGTTCTGGCCAGAGGACTTCTGCAGATACCCACGGGGCTCTGGACGACCATGGAGAAGAG TTCAACTTTGGAGACGTCTTTGTCCACCAAGCCATCCACACCATCGAGTACTGCCTGGGCTGCATTTCA AACACAGCCTCCTACCTGCGGCTCTGGGCCCTCAGCCTGGCTCATGCACAACTGTCTGAAGTGCTCTGG ACTATGGTGATGAACAGCGGCCTTCAGACGCGAGGCTGGGGAGGAATCGTCGGGGTTTTTATTATTTTT GCCGTATTTGCTGTCCTGACAGTAGCCATCCTTCTGATCATGGAGGGCCTCTCTGCTTTCCTGCACGCC CTGCGACTGCACTGGGTTGAGTTCCAGAACAAGTTCTATGTCGGGGATGGTTACAAGTTTTCTCCATTC TCCTTTAAACACATCCTGGATGGCACAGCCGAGGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020632 |
| Insert Size | 2523 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020632.2 |
| RefSeq Size | 3152 bp |
| RefSeq ORF | 2523 bp |
| Locus ID | 50617 |
| UniProt ID | Q9HBG4 |
| Protein Families | Transmembrane |
| Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
| MW | 96.4 kDa |
| Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) is the longest transcript. Variants 1, 2, and 3 encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212179 | ATP6V0A4 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1 |
CNY 6,152.00 |
|
| RC212179L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212179L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG212179 | ATP6V0A4 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal V0 subunit a4 (ATP6V0A4), transcript variant 1 |
CNY 7,752.00 |
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