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ASAH2 (NM_019893) Human Untagged Clone
CAT＃: SC304691
ASAH2 (untagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1
CNY 15,110.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BCDase; HNAC1; LCDase; N-CDase; NCDase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC304691 representing NM_019893.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCAAACGCACCTTCTCTAACTTGGAGACATTCCTGATTTTCCTCCTTGTAATGATGAGTGCCATC ACAGTGGCCCTTCTCAGCCTCTTGTTTATCACCAGTGGGACCATTGAAAACCACAAAGATTTAGGAGGC CATTTTTTTTCAACCACCCAAAGCCCTCCAGCCACCCAGGGCTCCACAGCTGCCCAACGCTCCACAGCC ACCCAGCATTCCACAGCCACCCAGAGCTCCACAGCCACTCAAACTTCTCCAGTGCCTTTAACCCCAGAG TCTCCTCTATTTCAGAACTTCAGTGGCTACCATATTGGTGTTGGACGAGCTGACTGCACAGGACAAGTA GCAGATATCAATTTGATGGGCTATGGCAAATCCGGCCAGAATGCACAGGGCATCCTCACCAGGCTATAC AGTCGTGCCTTCATCATGGCAGAACCTGATGGGTCCAATCGAACAGTGTTTGTCAGCATCGACATAGGC ATGGTATCACAAAGGCTCAGGCTGGAGGTCCTGAACAGACTGCAGAGTAAATATGGCTCCCTGTACAGA AGAGATAATGTCATCCTGAGTGGCACTCACACTCATTCAGGTCCTGCAGGATATTTCCAGTATACCGTG TTTGTAATTGCCAGTGAAGGATTTAGCAATCAAACTTTTCAGCACATGGTCACTGGTATCTTGAAGAGC ATTGACATAGCACACACAAATATGAAACCAGGCAAAATCTTCATCAATAAAGGAAATGTGGATGGTGTG CAGATCAACAGAAGTCCGTATTCTTACCTTCAAAATCCGCAGTCAGAGAGAGCAAGGTATTCTTCAAAT ACAGACAAGGAAATGATAGTTTTGAAAATGGTAGATTTGAATGGAGATGACTTGGGCCTTATCAGCTGG TTTGCCATCCACCCGGTCAGCATGAACAACAGTAACCATCTTGTAAACAGTGACAATGTGGGCTATGCA TCTTACCTGCTTGAGCAAGAGAAGAACAAAGGATATCTACCTGGACAGGGGCCATTTGTAGCAGCCTTT GCTTCATCAAACCTAGGAGATGTGTCCCCCAACATTCTTGGACCACGTTGCATCAACACAGGAGAGTCC TGTGATAACGCCAATAGCACTTGTCCCATTGGTGGGCCTAGCATGTGCATTGCTAAGGGACCTGGACAG GATATGTTTGACAGCACACAAATTATAGGACGGGCCATGTATCAGAGAGCAAAGGAACTCTATGCCTCT GCCTCCCAGGAGGTAACAGGACCACTGGCTTCAGCACACCAGTGGGTGGATATGACAGATGTGACTGTC TGGCTCAATTCCACACATGCATCAAAAACATGTAAACCAGCATTGGGCTACAGTTTTGCAGCTGGCACT ATTGATGGAGTTGGAGGCCTCAATTTTACACAGGGGAAAACAGAAGGGGATCCATTTTGGGACACCATT CGGGACCAGATCCTGGGAAAGCCATCTGAAGAAATTAAAGAATGTCATAAACCAAAGCCCATCCTTCTT CACACCGGAGAACTATCAAAACCTCACCCCTGGCATCCAGACATTGTTGATGTTCAGATTATTACCCTT GGGTCCTTGGCCATAACTGCCATCCCCGGGGAGTTTACGACCATGTCTGGACGAAGACTTCGAGAGGCA GTTCAAGCAGAATTTGCATCTCATGGGATGCAGAACATGACTGTTGTTATTTCAGGTCTATGCAACGTC TATACACATTACATTACCACTTATGAAGAATACCAGGCTCAGCGATATGAGGCAGCATCGACAATTTAT GGACCGCACACATTATCTGCTTACATTCAGCTCTTCAGAAACCTTGCTAAGGCTATTGCTACGGACACG GTAGCCAACCTGAGCAGAGGTCCAGAACCTCCCTTTTTCAAACAATTAATAGTTCCATTAATTCCTAGT ATTGTGGATAGAGCACCAAAAGGCAGAACTTTCGGGGATGTCCTGCAGCCAGCAAAACCTGAATACAGA GTGGGGGAAGTTGCTGAAGTTATATTTGTAGGTGCTAACCCGAAGAATTCAGTACAAAACCAGACCCAT CAGACCTTCCTCACTGTGGAGAAATATGAGGCTACTTCAACATCGTGGCAGATAGTGTGTAATGATGCC TCCTGGGAGACTCGTTTTTATTGGCACAAGGGACTCCTGGGTCTGAGTAATGCAACAGTGGAATGGCAT ATTCCAGACACTGCCCAGCCTGGAATCTACAGAATAAGATATTTTGGACACAATCGGAAGCAGGACATT CTGAAGCCTGCTGTCATACTTTCATTTGAAGGCACTTCCCCGGCTTTTGAAGTTGTAACTATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019893 |
| Insert Size | 2343 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019893.2 |
| RefSeq Size | 2432 bp |
| RefSeq ORF | 2343 bp |
| Locus ID | 56624 |
| UniProt ID | Q9NR71 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 85.5 kDa |
| Gene Summary | Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215027 | ASAH2 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 5,712.00 |
|
| RC215027L1 | Lenti-ORF clone of ASAH2 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 8,112.00 |
|
| RC215027L2 | Lenti-ORF clone of ASAH2 (mGFP-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 11,310.00 |
|
| RC215027L3 | Lenti-ORF clone of ASAH2 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 11,310.00 |
|
| RC215027L4 | Lenti-ORF clone of ASAH2 (mGFP-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 11,310.00 |
|
| RC229372 | ASAH2 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 9,410.00 |
|
| RC229372L3 | Lenti-ORF clone of ASAH2 (Myc-DDK-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 11,310.00 |
|
| RC229372L4 | Lenti-ORF clone of ASAH2 (mGFP-tagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 11,310.00 |
|
| RG215027 | ASAH2 (tGFP-tagged) - Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 10,360.00 |
|
| RG229372 | ASAH2 (tGFP-tagged) - Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 |
CNY 10,360.00 |
|
| SC328007 | ASAH2 (untagged)-Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2) transcript variant 1 |
CNY 15,110.00 |
