Myosin (MYH4) (NM_017533) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MYH2B; MyHC-2B; MyHC-IIb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304475 representing NM_017533. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTTCTGACTCTGAGATGGCCATTTTTGGGGAGGCTGCTCCTTTCCTCCGAAAGTCTGAAAAGGAG CGAATTGAAGCTCAGAACAAGCCTTTTGATGCCAAGACATCAGTCTTTGTGGTGGACCCTAAGGAGTCC TACGTGAAAGCAATAGTGCAGAGCAGGGAAGGGGGGAAGGTGACAGCCAAGACCGAAGCTGGAGCTACT GTAACTGTGAAAGAAGACCAAGTCTTCTCCATGAACCCTCCCAAATATGACAAGATCGAGGACATGGCC ATGATGACTCACCTGCATGAGCCTGCTGTGCTGTATAACCTCAAAGAGCGTTACGCAGCCTGGATGATC TACACCTACTCGGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCTGAG GTGGTGACAGCCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCATATCTTCTCCATCTCTGACAAT GCCTATCAGTTCATGCTAACTGATCGTGAAAACCAGTCAATCTTGATTACTGGAGAATCTGGTGCAGGG AAGACTGTGAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTTACTGGAGAGAAGAAAAAA GAGGAACCTGCCTCTGGCAAAATGCAGGGGACCCTTGAAGATCAAATCATCAGTGCTAACCCCCTACTG GAAGCCTTCGGCAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATC CATTTTGGTGCCACAGGCAAACTGGCTTCTGCAGATATTGAAACATATCTGCTAGAGAAGTCCCGAGTT ACTTTTCAGCTAAAGGCTGAAAGAAGCTACCACATATTTTATCAAATCCTGTCCAATAAGAAACCAGAG CTCATTGAAATGCTTCTGATCACCACCAACCCATATGACTTCGCATTTGTCAGCCAAGGGGAAATTACT GTGCCCAGCATTGATGACCAGGAAGAGCTGATGGCCACAGATAGTGCTGTGGACATCCTGGGTTTCACT GCTGATGAAAAGGTGGCCATTTACAAGCTCACTGGAGCCGTGATGCATTATGGGAACATGAAATTCAAG CAAAAGCAAAGGGAAGAGCAGGCAGAGCCAGATGGCACGGAAGTTGCTGACAAAGCTGCTTATCTGACA AGTCTGAACTCTGCTGACCTGCTCAAATCTCTCTGCTATCCCAGAGTCAAGGTCGGCAATGAGTTCGTA ACCAAAGGCCAGACTGTGCAGCAGGTGTACAATGCAGTGGGTGCTCTGGCCAAAGCCATCTACGAGAAG ATGTTCCTGTGGATGGTCACCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATC GGGGTCTTGGACATTGCTGGCTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTC ACCAACGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAAGAGTACAAGAAG GAAGGCATCGAGTGGGAGTTCATTGACTTCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAG CCTATGGGCATCTTCTCCATCCTAGAAGAGGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAG AACAAGCTGTATGAACAACATCTTGGAAAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAG CCTGAGGCTCACTTCTCACTGGTGCACTATGCCGGCACCGTGGACTACAACATCGCCGGCTGGCTGGAC AAAAACAAGGACCCCCTGAATGAGACTGTGGTGGGGCTGTACCAGAAGTCTGCAATGAAGACTCTGGCT TTCCTCTTCTCTGGGGCACAAACTGCTGAAGCAGAGGGTGGTGGTGGAAAGAAAGGTGGCAAAAAGAAG GGTTCTTCTTTCCAGACAGTGTCAGCTCTTTTCAGGGAGAATTTGAATAAGCTGATGACCAACTTGAGG AGCACTCACCCCCACTTTGTGCGGTGCATCATCCCCAATGAAACTAAAACTCCTGGTGCCATGGAGCAT GAGCTTGTCCTGCATCAGCTGAGGTGTAACGGTGTGCTGGAAGGCATCCGCATCTGCAGGAAAGGCTTC CCAAGCAGAATCCTTTATGCAGACTTCAAACAGAGATACAAGGTTCTAAATGCGAGTGCTATCCCAGAG GGTCAGTTCATTGACAGCAAGAAGGCTTCTGAGAAACTTCTAGGGTCTATTGAAATTGACCACACCCAG TACAAATTCGGTCATACCAAGGTTTTCTTCAAAGCTGGCCTGCTGGGAACTCTAGAGGAAATGCGAGAT GAAAAGCTAGCTCAACTCATCACGCGCACTCAAGCCATATGCAGAGGGTTCCTGATGAGAGTGGAGTTC AGAAAGATGATGGAGAGGAGAGAGTCCATCTTCTGCATTCAGTACAACATCCGTGCTTTCATGAATGTG AAGCACTGGCCCTGGATGAAGCTGTATTTCAAGATCAAGCCCCTCCTCAAGAGTGCAGAGACAGAGAAG GAGATGGCCAACATGAAGGAAGAATTTGAGAAAACCAAAGAAGAGCTGGCTAAGACAGAGGCAAAAAGG AAAGAACTAGAAGAAAAGATGGTGACGCTAATGCAAGAGAAAAATGACTTACAACTCCAAGTTCAAGCT GAAGCAGATGCCTTGGCTGATGCAGAGGAAAGATGTGATCAGTTGATTAAAACCAAAATCCAACTTGAG GCCAAAATCAAAGAGGTAACTGAAAGAGCTGAGGATGAGGAAGAGATCAATGCTGAGCTGACAGCCAAG AAGAGGAAACTGGAGGATGAATGTTCAGAGCTCAAGAAAGACATTGATGACCTTGAGCTGACACTGGCC AAGGTTGAGAAGGAGAAACATGCCACAGAGAACAAGGTGAAAAACCTCACAGAAGAGATGGCAGGTCTG GATGAAACCATTGCTAAGCTGACCAAGGAGAAGAAGGCTCTCCAGGAGGCCCACCAGCAGACCCTGGAT GACCTGCAGATGGAGGAGGACAAAGTCAACACCCTGACCAAAGCTAAAACCAAGCTAGAACAGCAAGTG GACGATCTTGAAGGATCTCTGGAACAAGAAAAGAAACTTTGCATGGACTTAGAAAGAGCCAAGAGAAAA CTGGAGGGTGACCTAAAATTGGCCCAAGAATCCACAATGGATACAGAAAATGACAAACAGCAACTTAAT GAGAAACTCAAAAAGAAAGAGTTTGAAATGAGCAATCTGCAAGGCAAGATTGAAGATGAACAAGCCCTT GCAATACAGCTACAAAAGAAGATCAAAGAATTACAGGCCCGCATTGAGGAGCTGGAGGAGGAAATCGAG GCAGAGCGGGCCTCCCGGGCCAAAGCAGAGAAGCAGCGCTCTGACCTCTCCCGGGAGCTGGAGGAGATC AGTGAGAGGCTGGAAGAAGCCGGTGGGGCCACTTCAGCCCAGATTGAGATGAACAAGAAGCGGGAGGCT GAGTTCCAGAAAATGCGCAGGGACCTGGAAGAGTCCACCCTGCAGCACGAAGCCACGGCAGCTGCTCTT CGGAAGAAGCACGCAGATAGTGTGGCTGAGCTTGGGGAGCAGATTGACAGCCTTCAGCGGGTCAAGCAG AAGCTGGAGAAGGAAAAGAGTGAGCTGAAGATGGAGATCAATGACCTTGCTAGTAACATGGAGACTGTC TCCAAAGCCAAGGCAAACTTTGAGAAAATGTGCCGCACCCTAGAGGACCAGCTTAGTGAAATAAAAACA AAGGAAGAAGAGCAACAACGCTTAATAAATGAGTTGTCAGCCCAGAAGGCACGTTTACACACAGAATCA GGTGAGTTTTCACGACAGCTAGATGAAAAAGATGCTATGGTTTCTCAGCTATCCCGAGGCAAACAAGCA TTTACACAACAGATTGAAGAATTAAAGAGGCAGCTAGAAGAGGAGACTAAGGCCAAGAGCACTCTGGCC CATGCCCTGCAGTCAGCCCGCCATGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAGCC AAGGCTGAGCTGCAGAGGGGAATGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGACCAAGTACGAG ACGGACGCCATCCAGCGCACAGAGGAGCTGGAGGAGGCCAAGAAGAAGCTAGCCCAGCGTCTGCAGGAT GCAGAAGAACATGTAGAAGCTGTGAATTCCAAATGTGCTTCTCTTGAAAAGACAAAGCAGAGGCTACAG AATGAAGTAGAGGACCTCATGATTGATGTGGAACGATCTAATGCTGCCTGCATAGCTCTCGATAAGAAG CAAAGAAACTTTGACAAGGTTCTGGCAGAATGGAAACAGAAGTATGAGGAAACTCAGGCTGAACTTGAG GCCTCCCAGAAGGAGTCGCGTTCTCTCAGCACTGAGCTGTTCAAGGTGAAGAATGCCTACGAGGAATCC CTGGATCATCTTGAAACTCTAAAGCGAGAGAATAAGAACTTACAACAGGAGATTTCTGACCTGACAGAG CAAATTGCAGAGGGTGGAAAGCATATCCATGAACTGGAGAAAGTAAAGAAACAACTTGATCATGAGAAG AGTGAACTACAGACTTCCCTAGAGGAAGCAGAGGCATCTCTTGAGCATGAAGAAGGCAAAATTCTTCGC ATTCAACTTGAGCTAAATCAGGTGAAATCTGAGATTGACCGAAAAATTGCTGAAAAAGATGAAGAACTC GATCAGCTAAAGAGGAACCATCTCAGAGTTGTGGAGTCAATGCAGAGTACACTGGATGCTGAGATCAGG AGCAGAAATGATGCTCTGAGGATCAAGAAGAAGATGGAGGGAGATCTTAATGAAATGGAAATCCAGCTG AACCATGCCAACCGCCAGGCTGCTGAGGCACTAAGGAATCTTAGAAACACACAAGGAATACTGAAGGAC ACTCAGCTACATTTGGATGATGCCATCAGAGGCCAAGATGACCTTAAGGAACAACTGGCAATGGTTGAG CGCAGAGCTAACCTGATGCAGGCTGAAGTTGAAGAGCTCAGGGCATCCCTGGAACGGACTGAGAGAGGC AGGAAAATGGCAGAGCAAGAGCTTCTGGATGCCAGTGAACGTGTGCAACTTCTGCACACTCAGAACACC AGCCTGATCAACACCAAGAAGAAGCTGGAAACAGACATTTCCCAAATCCAGGGAGAGATGGAGGACATC GTCCAGGAAGCCCGCAATGCAGAGGAGAAGGCCAAGAAGGCCATCACTGATGCTGCCATGATGGCTGAG GAGCTGAAGAAGGAACAGGACACCAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGTG AAGGATCTGCAGCTCCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGTGGGAAGAAGCAGATCCAG AAACTGGAGGCCAGGGTGAGAGAGCTTGAAAGTGAGGTGGAAAGTGAACAGAAGCACAATGTTGAGGCT GTCAAGGGTCTTCGCAAACATGAGAGAAGAGTGAAGGAACTCACTTACCAGACTGAGGAGGACCGCAAG AATATTCTCAGGCTGCAGGACTTGGTGGACAAATTGCAAACCAAAGTCAAAGCTTACAAGAGACAAGCT GAAGAGGCTGAGGAACAATCCAATGTCAACCTTGCCAAGTTCCGCAAGCTCCAGCACGAGCTGGAGGAG GCCAAGGAACGGGCTGACATTGCTGAGTCCCAAGTCAACAAGCTGAGAGTGAAGAGTCGGGAGGTTCAC ACAAAAGTCATAAGTGAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_017533
Insert Size	5820 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017533.2
RefSeq Size	6016 bp
RefSeq ORF	5820 bp
Locus ID	4622
UniProt ID	Q9Y623
Protein Pathways	Tight junction, Viral myocarditis
MW	223.1 kDa
Gene Summary	Muscle contraction.[UniProtKB/Swiss-Prot Function]

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