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DNA polymerase alpha (POLA1) (NM_016937) Human Untagged Clone
CAT＃: SC304453
POLA1 (untagged)-Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1)
CNY 28,220.00
货期*
2周
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NSX; p180; POLA; VEODS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_016937 edited
GACCATGGCACCTGTGCACGGCGACGACTCTCTGTCAGATTCAGGGAGTTTTGTATCTTC TCGAGCCCGGCGAGAAAAAAAATCAAAGAAGGGGCGCCAAGAAGCCCTAGAAAGACTGAA AAAGGCTAAAGCTGGTGAGAAGTATAAATATGAAGTCGAGGACTTCACAGGTGTTTATGA AGAAGTTGATGAAGAACAGTATTCGAAGCTGGTTCAGGCACGCCAGGATGATGACTGGAT TGTGGATGATGATGGTATTGGCTATGTGGAAGATGGCCGAGAGATTTTTGATGATGACCT TGAAGATGATGCCCTTGATGCTGATGAGAAAGGAAAAGATGGTAAAGCACGCAATAAAGA CAAGAGGAATGTAAAGAAGCTCGCAGTGACAAAACCGAACAACATTAAGTCAATGTTCAT TGCTTGTGCTGGAAAGAAAACTGCAGATAAAGCTGTAGACTTGTCCAAGGATGGTCTGCT AGGTGACATTCTACAGGATCTTAACACTGAGACACCTCAAATAACTCCACCACCTGTAAT GATACTGAAGAAGAAAAGATCCATTGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGC CACGGCAGTTCCTTCAGGAAAAATTGCTTCCCCTGTCTCCAGAAAGGAGCCTCCATTAAC TCCTGTTCCTCTTAAACGTGCTGAATTTGCTGGCGATGATGTACAGGTCGAGAGTACAGA AGAAGAGCAGGAGTCAGGGGCAATGGAGTTTGAAGATGGTGACTTTGATGAGCCCATGGA AGTTGAAGAGGTGGACCTGGAGCCTATGGCTGCCAAGGCTTGGGACAAAGAGAGTGAGCC AGCAGAGGAAGTGAAACAAGAGGCGGATTCTGGGAAAGGGACCGTGTCCTACTTAGGAAG TTTTCTCCCGGATGTCTCTTGTTGGGACATTGATCAAGAAGGTGATAGCAGTTTCTCAGT GCAAGAAGTTCAAGTGGATTCCAGTCACCTCCCATTGGTAAAAGGGGCAGATGAGGAACA AGTATTCCACTTTTATTGGTTGGATGCTTATGAGGATCAGTACAACCAACCAGGTGTGGT ATTTCTGTTTGGGAAAGTTTGGATTGAATCAGCCGAGACCCATGTGAGCTGTTGTGTCAT GGTGAAAAATATCGAGCGAACGCTTTACTTCCTTCCCCGTGAAATGAAAATTGATCTAAA TACGGGGAAAGAAACAGGAACTCCAATTTCAATGAAGGATGTTTATGAGGAATTTGATGA GAAAATAGCAACAAAATATAAAATTATGAAGTTCAAGTCTAAGCCAGTGGAAAAGAACTA TGCTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAAATACTCGGC TGAAATGCCACAGCTTCCTCAAGATTTGAAAGGAGAAACTTTTTCTCATGTATTTGGGAC CAACACATCTAGCCTGGAACTGTTCTTGATGAACAGAAAGATCAAAGGACCTTGTTGGCT TGAAGTAAAAAGTCCACAGCTCTTGAATCAGCCAGTCAGTTGGTGTAAAGTTGAGGCAAT GGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAGTCCACCACCGCTTGTCGT GATGGCTTTCAGCATGAAGACAATGCAGAATGCAAAGAACCATCAAAATGAGATTATTGC TATGGCAGCTTTGGTCCATCACAGTTTTGCATTGGATAAAGCAGCCCCAAAGCCTCCCTT TCAGTCACACTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTTCCATATGCTTTCAA AGAAGTCATTGAGAAAAAGAATGTGAAGGTTGAGGTTGCTGCAACAGAAAGAACACTGCT AGGTTTTTTCCTTGCAAAAGTTCACAAAATTGATCCTGATATCATTGTGGGTCATAATAT TTATGGGTTTGAACTGGAAGTACTACTGCAGAGAATTAATGTGTGCAAAGCTCCTCACTG GTCCAAGATAGGTCGACTGAAGCGATCCAACATGCCAAAGCTTGGGGGCCGGAGTGGATT TGGTGAAAGAAATGCTACCTGTGGTCGAATGATCTGTGATGTGGAAATTTCAGCAAAGGA ATTGATTCGTTGTAAAAGCTACCATCTGTCTGAACTTGTTCAGCAGATTCTAAAAACTGA AAGGGTTGTAATCCCAATGGAAAATATACAAAATATGTACAGTGAATCTTCTCAACTGTT ATACCTGTTGGAACACACCTGGAAAGATGCCAAGTTCATTTTGCAGATCATGTGTGAGCT AAATGTTCTTCCATTAGCATTGCAGATCACTAACATCGCTGGGAACATTATGTCCAGGAC GCTGATGGGTGGACGATCCGAGCGTAACGAGTTCTTGTTGCTTCATGCATTTTACGAAAA CAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAGCCTCAGCAAAAACTGGGAGATGA AGATGAAGAAATTGATGGAGATACCAATAAATACAAGAAAGGACGTAAGAAAGCAGCTTA TGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGATAAGTTCATTTTGCTTCT GGACTTCAACAGTCTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGT ACAAAGAGTTGCTTCAGAGGCACAGAAAGTTACAGAGGATGGAGAACAAGAACAGATCCC TGAGTTGCCAGATCCAAGCTTAGAAATGGGCATTTTGCCCAGAGAGATCCGGAAACTGGT AGAACGGAGAAAACAAGTCAAACAGCTAATGAAACAGCAAGACTTAAATCCAGACCTTAT TCTTCAGTATGACATTCGACAGAAGGCTTTGAAGCTCACAGCGAACAGTATGTATGGTTG CCTGGGATTTTCCTATAGCAGATTTTACGCCAAACCACTGGCTGCCTTGGTGACATACAA AGGAAGGGAGATTTTGATGCATACGAAAGAGATGGTACAAAAGATGAATCTTGAAGTTAT TTATGGAGATACAGATTCAATTATGATAAACACCAATAGCACCAATCTGGAAGAAGTATT TAAGTTGGGAAACAAGGTAAAAAGTGAAGTGAATAAGTTGTACAAACTGCTTGAAATAGA CATTGATGGGGTTTTCAAGTCTCTGCTACTGCTGAAAAAAAAGAAGTACGCTGCTCTGGT TGTTGAGCCAACGTCGGATGGGAATTATGTCACCAAACAGGAGCTCAAAGGATTAGATAT AGTTAGAAGAGATTGGTGTGATCTTGCTAAAGACACTGGAAACTTTGTGATTGGCCAGAT TCTTTCTGATCAAAGCCGGGACACTATAGTGGAAAACATTCAGAAGAGGCTGATAGAAAT TGGAGAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATT GACAAAGGATCCCCAGGATTACCCTGATAAAAAAAGCCTACCTCATGTACATGTTGCCCT CTGGATAAATTCTCAAGGAGGCAGAAAGGTGAAAGCTGGAGATACTGTGTCATATGTCAT CTGTCAGGATGGATCAAACCTCACTGCAAGTCAGAGGGCCTATGCGCCTGAGCAGCTGCA GAAACAGGATAATCTAACCATTGACACCCAGTACTACCTGGCCCAGCAGATCCACCCAGT CGTGGCTCGGATCTGTGAACCAATAGACGGAATTGATGCTGTCCTCATTGCAACGTGGTT GGGACTTGACCCCACCCAATTTAGAGTTCATCATTATCATAAAGATGAAGAGAATGATGC TCTACTTGGTGGCCCAGCACAGCTCACTGATGAAGAGAAATACAGGGACTGTGAAAGATT CAAATGTCCATGCCCTACATGTGGAACTGAGAATATTTATGATAATGTCTTTGATGGTTC GGGAACAGATATGGAGCCCAGCTTGTATCGTTGCAGTAACATCGATTGTAAGGCTTCACC TCTGACCTTTACAGTACAACTGAGCAACAAATTGATCATGGACATTAGACGTTTCATTAA AAAGTACTATGATGGCTGGTTGATATGTGAAGAGCCAACCTGTCGCAATCGAACTCGTCA CCTTCCCCTTCAATTCTCCCGAACTGGGCCTCTTTGCCCAGCCTGCATGAAAGCTACACT TCAACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGCTTTTACCGGTACATTTT TGATGCGGAGTGTGCACTGGAGAAACTTACTACCGATCATGAGAAAGATAAATTGAAGAA GCAATTTTTTACCCCCAAAGTTCTGCAGGACTACAGAAAACTCAAGAACACAGCAGAGCA ATTCTTGTCCCGAAGTGGCTACTCCGAAGTGAATCTGAGCAAACTCTTCGCTGGTTGTGC CGTGAAATCCTAAGGGAATCCCAGGAGTAACCAAGGAGGGGGTAGTTGAAAAATCCCAGC TTCCTCTGTGCCTCCACTCTGGCCCTAAATGCTCCTCCAGCATCTGTTTCTCCCTTGGGA CTGTGTCTCATGTTTGTGTGAATGTAGACCAGGAAAGGGGGCTGCAAAAATGTTGAGTCT AATGTTCGTAAGCATCATAGAAATTCCTGTCTTCATATTAAGATGTACTGCTTTAAAACA CAACTCCAGAGCCCCTCCCCAAGCTCCCCTCCCCAAGCTCCTGAAGACCCGGTTTCTGAG GGAGGGAAATTGCTACTTGGATTGAGAGTAGCTGGAATGTAAGTGACCCCAGGCTTTGCC TCAGGGCCTTTAGCCTATGTCCCCCCCACATAAAGAGAGCTTCTCAGAGCCTGACTGAAG AGCTGACGTTTTGCTTTTTCATATGCCAATTAAACCCGGTCTAAATCCAAATGCTTCTCC AGCCATCCAGGAGTGGCTGTCCTTTTCAGTCTTGTCTTTTATATAGGTAGCTGAGGGGGA AGATTTAGAAGCCTTGCACTCACTAAATAGATTAAACAGAGCAGGCTTGTTTGTTGAATT GCTCCAAAGTCCAACAGACACACACTGAGCAGGTGTTTTACACTCACATTCCCTTTTTGC CCCTTAAATAGAAAGTGCAGGTAAAGGTTTATACAACAAGAAAGCACATTGAAAATAATT TGATACTCTAACAATCCATTAACATGTGTAGGGGTTACGGTGAGGATCACTGTGTTGTAT TCAGAAAAACGGGGAGAGGGATGCTTAATTGGCCCTGGCGCTTGCTATTTTTTTCTCATT TCTTCACAATAGGACCGTCTTTGGCAGCAGCAAAATGTATTTCAGTATGGCAGTCTTTCC TCTCTTACATTATTGGTAAGATTATACTAACAAAATGTTTCCCCTTGTACAATTATGCTG TGTTTTTAAAAAACATTGACCTGTGTGTTTTTATAAAAGAAAAAGTATGTTGTGCCTTCT TCTTAAGAATAAAGTTTTCTAAAGGGAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_016937 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | It is not a varient. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016937.1, NP_058633.1 |
| RefSeq Size | 5433 bp |
| RefSeq ORF | 4389 bp |
| Locus ID | 5422 |
| UniProt ID | P09884 |
| Protein Families | Druggable Genome |
| Protein Pathways | DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism |
| Gene Summary | This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Methionine Mistranslation Bypasses the Restraint of the Genetic Code to Generate Mutant Proteins with Distinct Activities
,null,
PLoS Genetics
,PubMed ID 26709516
[POLA1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214011 | POLA1 (Myc-DDK-tagged)-Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1) |
CNY 15,008.00 |
|
| RC214011L3 | Lenti-ORF clone of POLA1 (Myc-DDK-tagged)-Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1) |
CNY 16,530.00 |
|
| RC214011L4 | Lenti-ORF clone of POLA1 (mGFP-tagged)-Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1) |
CNY 16,530.00 |
|
| RG214011 | POLA1 (tGFP-tagged) - Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1) |
CNY 16,060.00 |
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