KIF20B (NM_016195) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT90; KRMP1; MPHOSPH1; MPP-1; MPP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC304391 representing NM_016195. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAATCTAATTTTAATCAAGAGGGAGTACCTCGACCATCTTATGTTTTTAGTGCTGACCCAATTGCA AGGCCTTCAGAAATAAATTTCGATGGCATTAAGCTTGATCTGTCTCATGAATTTTCCTTAGTTGCTCCA AATACTGAGGCAAACAGTTTCGAATCTAAAGATTATCTCCAGGTTTGTCTTCGAATAAGACCATTTACA CAGTCAGAAAAAGAACTTGAGTCTGAGGGCTGTGTGCATATTCTGGATTCACAGACTGTTGTGCTGAAA GAGCCTCAATGCATCCTTGGTCGGTTAAGTGAAAAAAGCTCAGGGCAGATGGCACAGAAATTCAGTTTT TCCAAGGTTTTTGGCCCAGCAACTACACAGAAGGAATTCTTTCAGGGTTGCATTATGCAACCAGTAAAA GACCTCTTGAAAGGACAGAGTCGTCTGATTTTTACTTACGGGCTAACCAATTCAGGAAAAACATATACA TTTCAAGGGACAGAAGAAAATATTGGCATTCTGCCTCGAACTTTGAATGTATTATTTGATAGTCTTCAA GAAAGACTGTATACAAAGATGAACCTTAAACCACATAGATCCAGAGAATACTTAAGGTTATCATCAGAA CAAGAGAAAGAAGAAATTGCTAGCAAAAGTGCATTGCTTCGGCAAATTAAAGAGGTTACTGTGCATAAT GATAGTGATGATACTCTTTATGGAAGTTTAACTAACTCTTTGAATATCTCAGAGTTTGAAGAATCCATA AAAGATTATGAACAAGCCAACTTGAATATGGCTAATAGTATAAAATTTTCTGTGTGGGTTTCTTTCTTT GAAATTTACAATGAATATATTTATGACTTATTTGTTCCTGTATCATCTAAATTCCAAAAGAGAAAGATG CTGCGCCTTTCCCAAGACGTAAAGGGCTATTCTTTTATAAAAGATCTACAATGGATTCAAGTATCTGAT TCCAAAGAAGCCTATAGACTTTTAAAACTAGGAATAAAGCACCAGAGTGTTGCCTTCACAAAATTGAAT AATGCTTCCAGTAGAAGTCACAGCATATTCACTGTTAAAATATTACAGATTGAAGATTCTGAAATGTCT CGTGTAATTCGAGTCAGTGAATTATCTTTATGTGATCTTGCTGGTTCAGAACGAACTATGAAGACACAG AATGAAGGTGAAAGGTTAAGAGAGACTGGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATT AACGTCTTGAAGAATAGTGAAAAGTCAAAGTTTCAACAGCATGTGCCTTTCCGGGAAAGTAAACTGACT CACTATTTTCAAAGTTTTTTTAATGGTAAAGGGAAAATTTGTATGATTGTCAATATCAGCCAATGTTAT TTAGCCTATGATGAAACACTCAATGTATTGAAGTTCTCCGCCATTGCACAAAAAGTTTGTGTCCCAGAC ACTTTAAATTCCTCTCAAGAGAAATTATTTGGACCTGTCAAATCTTCTCAAGATGTATCACTAGACAGT AATTCAAACAGTAAAATATTAAATGTAAAAAGAGCCACCATTTCATGGGAAAATAGTCTAGAAGATTTG ATGGAAGACGAGGATTTGGTTGAGGAGCTAGAAAACGCTGAAGAAACTCAAAATGTGGAAACTAAACTT CTTGATGAAGATCTAGATAAAACATTAGAGGAAAATAAGGCTTTCATTAGCCACGAGGAGAAAAGAAAA CTGTTGGACTTAATAGAAGACTTGAAAAAAAAACTGATAAATGAAAAAAAGGAAAAATTAACCTTGGAA TTTAAAATTCGAGAAGAAGTTACACAGGAGTTTACTCAGTATTGGGCTCAACGGGAAGCTGACTTTAAG GAGACTCTGCTTCAAGAACGAGAGATATTAGAAGAAAATGCTGAACGTCGTTTGGCTATCTTCAAGGAT TTGGTTGGTAAATGTGACACTCGAGAAGAAGCAGCGAAAGACATTTGTGCCACAAAAGTTGAAACTGAA GAAGCTACTGCTTGTTTAGAACTAAAGTTTAATCAAATTAAAGCTGAATTAGCTAAAACCAAAGGAGAA TTAATCAAAACCAAAGAAGAGTTAAAAAAGAGAGAAAATGAATCAGATTCATTGATTCAAGAGCTTGAG ACATCTAATAAGAAAATAATTACACAGAATCAAAGAATTAAAGAATTGATAAATATAATTGATCAAAAA GAAGATACTATCAACGAATTTCAGAACCTAAAGTCTCATATGGAAAACACATTTAAATGCAATGACAAG GCTGATACATCTTCTTTAATAATAAACAATAAATTGATTTGTAATGAAACAGTTGAAGTACCTAAGGAC AGCAAATCTAAAATCTGTTCAGAAAGAAAAAGAGTAAATGAAAATGAACTTCAGCAAGATGAACCACCA GCAAAGAAAGGGTCTATCCATGTTAGTTCAGCTATCACTGAAGACCAAAAGAAAAGTGAAGAAGTGCGA CCGAACATTGCAGAAATTGAAGACATCAGAGTTTTACAAGAAAATAATGAAGGACTGAGAGCATTTTTA CTCACTATTGAGAATGAACTTAAAAATGAAAAGGAAGAAAAAGCAGAATTAAATAAACAGATTGTTCAT TTTCAGCAGGAACTTTCTCTTTCTGAAAAAAAGAATTTAACTTTAAGTAAAGAGGTCCAACAAATTCAG TCAAATTATGATATTGCAATTGCTGAATTACATGTGCAGAAAAGTAAAAATCAAGAACAGGAGGAAAAG ATCATGAAATTGTCAAATGAGATAGAAACTGCTACAAGAAGCATTACAAATAATGTTTCACAAATAAAA TTAATGCACACGAAAATAGACGAACTACGTACTCTTGATTCAGTTTCTCAGATTTCAAACATAGATTTG CTCAATCTCAGGGATCTGTCAAATGGTTCTGAGGAGGATAATTTGCCAAATACACAGTTAGACCTTTTA GGTAATGATTATTTGGTAAGTAAGCAAGTTAAAGAATATCGAATTCAAGAACCCAATAGGGAAAATTCT TTCCACTCTAGTATTGAAGCTATTTGGGAAGAATGTAAAGAGATTGTGAAGGCCTCTTCCAAAAAAAGT CATCAGATTGAGGAACTGGAACAACAAATTGAAAAATTGCAGGCAGAAGTAAAAGGCTATAAGGATGAA AACAATAGACTAAAGGAGAAGGAGCATAAAAACCAAGATGACCTACTAAAAGAAAAAGAAACTCTTATA CAGCAGCTGAAAGAAGAATTGCAAGAAAAAAATGTTACTCTTGATGTTCAAATACAGCATGTAGTTGAA GGAAAGAGAGCGCTTTCAGAACTTACACAAGGTGTTACTTGCTATAAGGCAAAAATAAAGGAACTTGAA ACAATTTTAGAGACTCAGAAAGTTGAATGTAGTCATTCAGCCAAGTTAGAACAAGACATTTTGGAAAAG GAATCTATCATCTTAAAGCTAGAAAGAAATTTGAAGGAATTTCAAGAACATCTTCAGGATTCTGTCAAA AACACCAAAGATTTAAATGTAAAGGAACTCAAGCTGAAAGAAGAAATCACACAGTTAACAAATAATTTG CAAGATATGAAACATTTACTTCAATTAAAAGAAGAAGAAGAAGAAACCAACAGGCAAGAAACAGAAAAA TTGAAAGAGGAACTCTCTGCAAGCTCTGCTCGTACCCAGAATCTGAAAGCAGATCTTCAGAGGAAGGAA GAAGATTATGCTGACCTGAAAGAGAAACTGACTGATGCCAAAAAGCAGATTAAGCAAGTACAGAAAGAG GTATCTGTAATGCGTGATGAGGATAAATTACTGAGGATTAAAATTAATGAACTGGAGAAAAAGAAAAAC CAGTGTTCTCAGGAATTAGATATGAAACAGCGAACCATTCAGCAACTCAAGGAGCAGTTAAATAATCAG AAAGTGGAAGAAGCTATACAACAGTATGAGAGAGCATGCAAAGATCTAAATGTTAAAGAGAAAATAATT GAAGACATGCGAATGACACTAGAAGAACAGGAACAAACTCAGGTAGAACAGGATCAAGTGCTTGAGGCT AAATTAGAGGAAGTTGAAAGGCTGGCCACAGAATTGGAAAAATGGAAGGAAAAATGCAATGATTTGGAA ACCAAAAACAATCAAAGGTCAAATAAAGAACATGAGAACAACACAGATGTGCTTGGAAAGCTCACTAAT CTTCAAGATGAGTTACAGGAGTCTGAACAGAAATATAATGCTGATAGAAAGAAATGGTTAGAAGAAAAA ATGATGCTTATCACTCAAGCGAAAGAAGCAGAGAATATACGAAATAAAGAGATGAAAAAATATGCTGAG GACAGGGAGCGTTTTTTTAAGCAACAGAATGAAATGGAAATACTGACAGCCCAGCTGACAGAGAAAGAT AGTGACCTTCAAAAGTGGCGAGAAGAACGAGATCAACTGGTTGCAGCTTTAGAAATACAGCTAAAAGCA CTGATATCCAGTAATGTACAGAAAGATAATGAAATTGAACAACTAAAAAGGATCATATCAGAGACTTCT AAAATAGAAACACAAATCATGGATATCAAGCCCAAACGTATTAGTTCAGCAGATCCTGACAAACTTCAA ACTGAACCTCTATCGACAAGTTTTGAAATTTCCAGAAATAAAATAGAGGATGGATCTGTAGTCCTTGAC TCTTGTGAAGTGTCAACAGAAAATGATCAAAGCACTCGATTTCCAAAACCTGAGTTAGAGATTCAATTT ACACCTTTACAGCCAAACAAAATGGCAGTGAAACACCCTGGTTGTACCACACCAGTGACAGTTAAGATT CCCAAGGCTCGGAAGAGGAAGAGTAATGAAATGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAAT GCTACACCCAGAACTAATTTGAAATTTCCTATTTCAGATGATAGAAATTCTTCTGTCAAAAAGGAACAA AAGGTTGCCATACGTCCATCATCTAAGAAAACATATTCTTTACGGAGTCAGGCATCCATAATTGGTGTA AACCTGGCCACTAAGAAAAAAGAAGGAACACTACAGAAATTTGGAGACTTCTTACAACATTCTCCCTCA ATTCTTCAATCAAAAGCAAAGAAGATAATTGAAACAATGAGCTCTTCAAAGCTCTCAAATGTAGAAGCA AGTAAAGAAAATGTGTCTCAACCAAAACGAGCCAAACGGAAATTATACACAAGTGAAATTTCATCTCCT ATTGATATATCAGGCCAAGTGATTTTAATGGACCAGAAAATGAAGGAGAGTGATCACCAGATTATCAAA CGACGACTTCGAACAAAAACAGCCAAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_016195
Insert Size	5343 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016195.3
RefSeq Size	6339 bp
RefSeq ORF	5343 bp
Locus ID	9585
UniProt ID	Q96Q89
Protein Families	Druggable Genome
MW	206.1 kDa
Gene Summary	Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to isoform 1. The encoded isoform (2) is shorter compared to isoform 1.

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