KIF20B (NM_016195) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CT90; KRMP1; MPHOSPH1; MPP-1; MPP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC215061 representing NM_016195 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATCTAATTTTAATCAAGAGGGAGTACCTCGACCATCTTATGTTTTTAGTGCTGACCCAATTGCAA GGCCTTCAGAAATAAATTTCGATGGCATTAAGCTTGATCTGTCTCATGAATTTTCCTTAGTTGCTCCAAA TACTGAGGCAAACAGTTTCGAATCTAAAGATTATCTCCAGGTTTGTCTTCGAATAAGACCATTTACACAG TCAGAAAAAGAACTTGAGTCTGAGGGCTGTGTGCATATTCTGGATTCACAGACTGTTGTGCTGAAAGAGC CTCAATGCATCCTTGGTCGGTTAAGTGAAAAAAGCTCAGGGCAGATGGCACAGAAATTCAGTTTTTCCAA GGTTTTTGGCCCAGCAACTACACAGAAGGAATTCTTTCAGGGTTGCATTATGCAACCAGTAAAAGACCTC TTGAAAGGACAGAGTCGTCTGATTTTTACTTACGGGCTAACCAATTCAGGAAAAACATATACATTTCAAG GGACAGAAGAAAATATTGGCATTCTGCCTCGAACTTTGAATGTATTATTTGATAGTCTTCAAGAAAGACT GTATACAAAGATGAACCTTAAACCACATAGATCCAGAGAATACTTAAGGTTATCATCAGAACAAGAGAAA GAAGAAATTGCTAGCAAAAGTGCATTGCTTCGGCAAATTAAAGAGGTTACTGTGCATAATGATAGTGATG ATACTCTTTATGGAAGTTTAACTAACTCTTTGAATATCTCAGAGTTTGAAGAATCCATAAAAGATTATGA ACAAGCCAACTTGAATATGGCTAATAGTATAAAATTTTCTGTGTGGGTTTCTTTCTTTGAAATTTACAAT GAATATATTTATGACTTATTTGTTCCTGTATCATCTAAATTCCAAAAGAGAAAGATGCTGCGCCTTTCCC AAGACGTAAAGGGCTATTCTTTTATAAAAGATCTACAATGGATTCAAGTATCTGATTCCAAAGAAGCCTA TAGACTTTTAAAACTAGGAATAAAGCACCAGAGTGTTGCCTTCACAAAATTGAATAATGCTTCCAGTAGA AGTCACAGCATATTCACTGTTAAAATATTACAGATTGAAGATTCTGAAATGTCTCGTGTAATTCGAGTCA GTGAATTATCTTTATGTGATCTTGCTGGTTCAGAACGAACTATGAAGACACAGAATGAAGGTGAAAGGTT AAGAGAGACTGGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATTAACGTCTTGAAGAATAGT GAAAAGTCAAAGTTTCAACAGCATGTGCCTTTCCGGGAAAGTAAACTGACTCACTATTTTCAAAGTTTTT TTAATGGTAAAGGGAAAATTTGTATGATTGTCAATATCAGCCAATGTTATTTAGCCTATGATGAAACACT CAATGTATTGAAGTTCTCCGCCATTGCACAAAAAGTTTGTGTCCCAGACACTTTAAATTCCTCTCAAGAG AAATTATTTGGACCTGTCAAATCTTCTCAAGATGTATCACTAGACAGTAATTCAAACAGTAAAATATTAA ATGTAAAAAGAGCCACCATTTCATGGGAAAATAGTCTAGAAGATTTGATGGAAGACGAGGATTTGGTTGA GGAGCTAGAAAACGCTGAAGAAACTCAAAATGTGGAAACTAAACTTCTTGATGAAGATCTAGATAAAACA TTAGAGGAAAATAAGGCTTTCATTAGCCACGAGGAGAAAAGAAAACTGTTGGACTTAATAGAAGACTTGA AAAAAAAACTGATAAATGAAAAAAAGGAAAAATTAACCTTGGAATTTAAAATTCGAGAAGAAGTTACACA GGAGTTTACTCAGTATTGGGCTCAACGGGAAGCTGACTTTAAGGAGACTCTGCTTCAAGAACGAGAGATA TTAGAAGAAAATGCTGAACGTCGTTTGGCTATCTTCAAGGATTTGGTTGGTAAATGTGACACTCGAGAAG AAGCAGCGAAAGACATTTGTGCCACAAAAGTTGAAACTGAAGAAGCTACTGCTTGTTTAGAACTAAAGTT TAATCAAATTAAAGCTGAATTAGCTAAAACCAAAGGAGAATTAATCAAAACCAAAGAAGAGTTAAAAAAG AGAGAAAATGAATCAGATTCATTGATTCAAGAGCTTGAGACATCTAATAAGAAAATAATTACACAGAATC AAAGAATTAAAGAATTGATAAATATAATTGATCAAAAAGAAGATACTATCAACGAATTTCAGAACCTAAA GTCTCATATGGAAAACACATTTAAATGCAATGACAAGGCTGATACATCTTCTTTAATAATAAACAATAAA TTGATTTGTAATGAAACAGTTGAAGTACCTAAGGACAGCAAATCTAAAATCTGTTCAGAAAGAAAAAGAG TAAATGAAAATGAACTTCAGCAAGATGAACCACCAGCAAAGAAAGGGTCTATCCATGTTAGTTCAGCTAT CACTGAAGACCAAAAGAAAAGTGAAGAAGTGCGACCGAACATTGCAGAAATTGAAGACATCAGAGTTTTA CAAGAAAATAATGAAGGACTGAGAGCATTTTTACTCACTATTGAGAATGAACTTAAAAATGAAAAGGAAG AAAAAGCAGAATTAAATAAACAGATTGTTCATTTTCAGCAGGAACTTTCTCTTTCTGAAAAAAAGAATTT AACTTTAAGTAAAGAGGTCCAACAAATTCAGTCAAATTATGATATTGCAATTGCTGAATTACATGTGCAG AAAAGTAAAAATCAAGAACAGGAGGAAAAGATCATGAAATTGTCAAATGAGATAGAAACTGCTACAAGAA GCATTACAAATAATGTTTCACAAATAAAATTAATGCACACGAAAATAGACGAACTACGTACTCTTGATTC AGTTTCTCAGATTTCAAACATAGATTTGCTCAATCTCAGGGATCTGTCAAATGGTTCTGAGGAGGATAAT TTGCCAAATACACAGTTAGACCTTTTAGGTAATGATTATTTGGTAAGTAAGCAAGTTAAAGAATATCGAA TTCAAGAACCCAATAGGGAAAATTCTTTCCACTCTAGTATTGAAGCTATTTGGGAAGAATGTAAAGAGAT TGTGAAGGCCTCTTCCAAAAAAAGTCATCAGATTGAGGAACTGGAACAACAAATTGAAAAATTGCAGGCA GAAGTAAAAGGCTATAAGGATGAAAACAATAGACTAAAGGAGAAGGAGCATAAAAACCAAGATGACCTAC TAAAAGAAAAAGAAACTCTTATACAGCAGCTGAAAGAAGAATTGCAAGAAAAAAATGTTACTCTTGATGT TCAAATACAGCATGTAGTTGAAGGAAAGAGAGCGCTTTCAGAACTTACACAAGGTGTTACTTGCTATAAG GCAAAAATAAAGGAACTTGAAACAATTTTAGAGACTCAGAAAGTTGAATGTAGTCATTCAGCCAAGTTAG AACAAGACATTTTGGAAAAGGAATCTATCATCTTAAAGCTAGAAAGAAATTTGAAGGAATTTCAAGAACA TCTTCAGGATTCTGTCAAAAACACCAAAGATTTAAATGTAAAGGAACTCAAGCTGAAAGAAGAAATCACA CAGTTAACAAATAATTTGCAAGATATGAAACATTTACTTCAATTAAAAGAAGAAGAAGAAGAAACCAACA GGCAAGAAACAGAAAAATTGAAAGAGGAACTCTCTGCAAGCTCTGCTCGTACCCAGAATCTGAAAGCAGA TCTTCAGAGGAAGGAAGAAGATTATGCTGACCTGAAAGAGAAACTGACTGATGCCAAAAAGCAGATTAAG CAAGTACAGAAAGAGGTATCTGTAATGCGTGATGAGGATAAATTACTGAGGATTAAAATTAATGAACTGG AGAAAAAGAAAAACCAGTGTTCTCAGGAATTAGATATGAAACAGCGAACCATTCAGCAACTCAAGGAGCA GTTAAATAATCAGAAAGTGGAAGAAGCTATACAACAGTATGAGAGAGCATGCAAAGATCTAAATGTTAAA GAGAAAATAATTGAAGACATGCGAATGACACTAGAAGAACAGGAACAAACTCAGGTAGAACAGGATCAAG TGCTTGAGGCTAAATTAGAGGAAGTTGAAAGGCTGGCCACAGAATTGGAAAAATGGAAGGAAAAATGCAA TGATTTGGAAACCAAAAACAATCAAAGGTCAAATAAAGAACATGAGAACAACACAGATGTGCTTGGAAAG CTCACTAATCTTCAAGATGAGTTACAGGAGTCTGAACAGAAATATAATGCTGATAGAAAGAAATGGTTAG AAGAAAAAATGATGCTTATCACTCAAGCGAAAGAAGCAGAGAATATACGAAATAAAGAGATGAAAAAATA TGCTGAGGACAGGGAGCGTTTTTTTAAGCAACAGAATGAAATGGAAATACTGACAGCCCAGCTGACAGAG AAAGATAGTGACCTTCAAAAGTGGCGAGAAGAACGAGATCAACTGGTTGCAGCTTTAGAAATACAGCTAA AAGCACTGATATCCAGTAATGTACAGAAAGATAATGAAATTGAACAACTAAAAAGGATCATATCAGAGAC TTCTAAAATAGAAACACAAATCATGGATATCAAGCCCAAACGTATTAGTTCAGCAGATCCTGACAAACTT CAAACTGAACCTCTATCGACAAGTTTTGAAATTTCCAGAAATAAAATAGAGGATGGATCTGTAGTCCTTG ACTCTTGTGAAGTGTCAACAGAAAATGATCAAAGCACTCGATTTCCAAAACCTGAGTTAGAGATTCAATT TACACCTTTACAGCCAAACAAAATGGCAGTGAAACACCCTGGTTGTACCACACCAGTGACAGTTAAGATT CCCAAGGCTCGGAAGAGGAAGAGTAATGAAATGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATG CTACACCCAGAACTAATTTGAAATTTCCTATTTCAGATGATAGAAATTCTTCTGTCAAAAAGGAACAAAA GGTTGCCATACGTCCATCATCTAAGAAAACATATTCTTTACGGAGTCAGGCATCCATAATTGGTGTAAAC CTGGCCACTAAGAAAAAAGAAGGAACACTACAGAAATTTGGAGACTTCTTACAACATTCTCCCTCAATTC TTCAATCAAAAGCAAAGAAGATAATTGAAACAATGAGCTCTTCAAAGCTCTCAAATGTAGAAGCAAGTAA AGAAAATGTGTCTCAACCAAAACGAGCCAAACGGAAATTATACACAAGTGAAATTTCATCTCCTATTGAT ATATCAGGCCAAGTGATTTTAATGGACCAGAAAATGAAGGAGAGTGATCACCAGATTATCAAACGACGAC TTCGAACAAAAACAGCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC215061 representing NM_016195 Red=Cloning site Green=Tags(s) MESNFNQEGVPRPSYVFSADPIARPSEINFDGIKLDLSHEFSLVAPNTEANSFESKDYLQVCLRIRPFTQ SEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKVFGPATTQKEFFQGCIMQPVKDL LKGQSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLFDSLQERLYTKMNLKPHRSREYLRLSSEQEK EEIASKSALLRQIKEVTVHNDSDDTLYGSLTNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYN EYIYDLFVPVSSKFQKRKMLRLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSR SHSIFTVKILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSLLTLGKCINVLKNS EKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVCVPDTLNSSQE KLFGPVKSSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELENAEETQNVETKLLDEDLDKT LEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFKIREEVTQEFTQYWAQREADFKETLLQEREI LEENAERRLAIFKDLVGKCDTREEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKK RENESDSLIQELETSNKKIITQNQRIKELINIIDQKEDTINEFQNLKSHMENTFKCNDKADTSSLIINNK LICNETVEVPKDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIRVL QENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQ KSKNQEQEEKIMKLSNEIETATRSITNNVSQIKLMHTKIDELRTLDSVSQISNIDLLNLRDLSNGSEEDN LPNTQLDLLGNDYLVSKQVKEYRIQEPNRENSFHSSIEAIWEECKEIVKASSKKSHQIEELEQQIEKLQA EVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYK AKIKELETILETQKVECSHSAKLEQDILEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEIT QLTNNLQDMKHLLQLKEEEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIK QVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDLNVK EKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRSNKEHENNTDVLGK LTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKYAEDRERFFKQQNEMEILTAQLTE KDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKL QTEPLSTSFEISRNKIEDGSVVLDSCEVSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKI PKARKRKSNEMEEDLVKCENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQASIIGVN LATKKKEGTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKLYTSEISSPID ISGQVILMDQKMKESDHQIIKRRLRTKTAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_016195
ORF Size	5340 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_016195.4
RefSeq Size	6319 bp
RefSeq ORF	5343 bp
Locus ID	9585
UniProt ID	Q96Q89
Protein Families	Druggable Genome
MW	206 kDa
Gene Summary	Plus-end-directed motor enzyme that is required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth (By similarity). Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).[UniProtKB/Swiss-Prot Function]

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