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PPAR gamma (PPARG) (NM_015869) Human Untagged Clone
CAT＃: SC304361
PPARG (untagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2
CN¥ 5,648.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_015869 edited
ATGGGTGAAACTCTGGGAGATTCTCCTATTGACCCAGAAAGCGATTCCTTCACTGATACA CTGTCTGCAAACATATCACAAGAAATGACCATGGTTGACACAGAGATGCCATTCTGGCCC ACCAACTTTGGGATCAGCTCCGTGGATCTCTCCGTAATGGAAGACCACTCCCACTCCTTT GATATCAAGCCCTTCACTACTGTTGACTTCTCCAGCATTTCTACTCCACATTACGAAGAC ATTCCATTCACAAGAACAGATCCAGTGGTTGCAGATTACAAGTATGACCTGAAACTTCAA GAGTACCAAAGTGCAATCAAAGTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACT CAGCTCTACAATAAGCCTCATGAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGT GTCTGTGGAGATAAAGCTTCTGGATTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAG GGTTTCTTCCGGAGAACAATCAGATTGAAGCTTATCTATGACAGATGTGATCTTAACTGT CGGATCCACAAAAAAAGTAGAAATAAATGTCAGTACTGTCGGTTTCAGAAATGCCTTGCA GTGGGGATGTCTCATAATGCCATCAGGTTTGGGCGGATGCCACAGGCCGAGAAGGAGAAG CTGTTGGCGGAGATCTCCAGTGATATCGACCAGCTGAATCCAGAGTCCGCTGACCTCCGG GCCCTGGCAAAACATTTGTATGACTCATACATAAAGTCCTTCCCGCTGACCAAAGCAAAG GCGAGGGCGATCTTGACAGGAAAGACAACAGACAAATCACCATTCGTTATCTATGACATG AATTCCTTAATGATGGGAGAAGATAAAATCAAGTTCAAACACATCACCCCCCTGCAGGAG CAGAGCAAAGAGGTGGCCATCCGCATCTTTCAGGGCTGCCAGTTTCGCTCCGTGGAGGCT GTGCAGGAGATCACAGAGTATGCCAAAAGCATTCCTGGTTTTGTAAATCTTGACTTGAAC GACCAAGTAACTCTCCTCAAATATGGAGTCCACGAGATCATTTACACAATGCTGGCCTCC TTGATGAATAAAGATGGGGTTCTCATATCCGAGGGCCAAGGCTTCATGACAAGGGAGTTT CTAAAGAGCCTGCGAAAGCCTTTTGGTGACTTTATGGAGCCCAAGTTTGAGTTTGCTGTG AAGTTCAATGCACTGGAATTAGATGACAGCGACTTGGCAATATTTATTGCTGTCATTATT CTCAGTGGAGACCGCCCAGGTTTGCTGAATGTGAAGCCCATTGAAGACATTCAAGACAAC CTGCTACAAGCCCTGGAGCTCCAGCTGAAGCTGAACCACCCTGAGTCCTCACAGCTGTTT GCCAAGCTGCTCCAGAAAATGACAGACCTCAGACAGATTGTCACGGAACACGTGCAGCTA CTGCAGGTGATCAAGAAGACGGAGACAGACATGAGTCTTCACCCGCTCCTGCAGGAGATC TACAAGGACTTGTACTAG >OriGene 5' read for NM_015869 unedited
NCGTCAGATTTGTATACGACTCACTATAGGCGGCCGCGATTCAAATCTGGTACCGAGCTC GGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCGGATTGATCTTTTGCT AGATAGAGACAAAATATCAGTGTGAATTACAGCAAACCCCTATTCCATGCTGTTATGGGT GAAACTCTGGGAGATTCTCCTATTGACCCAGAAAGCGATTCCTTCACTGATACACTGTCT GCAAACATATCACAAGAAATGACCATGGTTGACACAGAGATGCCATTCTGGCCCACCAAC TTTGGGATCAGCTCCGTGGATCTCTCCGTAATGGAAGACCACTCCCACTCCTTTGATATC AAGCCCTTCACTACTGTTGACTTCTCCAGCATTTCTACTCCACATTACGAAGACATTCCA TTCACAAGAACAGATCCAGTGGTTGCAGATTACAAGTATGACCTGAAACTTCAAGAGTAC CAAAGTGCAATCAAAGTGGAGCCTGCATCTCCACCTTATTATTCTGAGAAGACTCAGCTC TACAATAAGCCTCATGAAGAGCCTTCCAACTCCCTCATGGCAATTGAATGTCGTGTCTGT GGAGATAAAGCTTCTGGATTTCACTATGGAGTTCATGCTTGTGAAGGATGCAAGGGTTTC TTCCGGAGAACAATCAGATTGAAGCTTATCTATGACAGATGTGATCTTAACTGTCGGATC CACAAAAAAAGTAGAAATAAATGTCAGTACTGTCGGTTTCAGAAATGCCTTGCAGTGGGG ATGTCTCATATGCCATCAGGTTTGGGCGGATGCCCACAGCCGAGAAGGAGAAGCTNGTTG GCGAGATCTCCAGTGATATCGACCAGCTGAATCCAGAGTCCGCTGACCTCCGGNNCCCTG NCAAACATTTGTATGA >Forward primer walk for NM_015869 unedited
NNNNCCCGGGGGGTGCAGGTGATACCANGATATGCCAAAGACTTCCTGGTTTTGTAAATC TTGACTTGAACGACCAAGTAACTCTCCTCAATATGGAGTCCACGAGATCATTTACACAAT GCTGGCCTCCTTGATGAATAAAGATGGGGTTCTCATATCCGAGGGCCAAGGCTTCATGAC AAGGGAGTTTCTAAAGAGCCTGCGAAAGCCTTTTGGTGACTTTATGGAGCCCAAGTTTGA GTTTGCTGTGAAGTTCAATGCACTGGAATTAGATGACAGCGACTTGGCAATATTTATTGC TGTCATTATTCTCAGTGGAGACCGCCCAGGTTTGCTGAATGTGAAGCCCATTGAAGACAT TCAAGACAACCTGCTACAAGCCCTGGAGCTCCAGCTGAAGCTGAACCACCCTGAGTCCTC ACAGCTGTTTGCCAAGCTGCTCCAGANAATGACAGACCTCAGACAGATTGTCACGGAACA CGTGCAGCTACTGCAGGTGATCAAGAAGACGGAGACAGACATGAGTCTTCACCCGCTCCT GCANGAGATCTACAAGGACTTGTACTAGCAGAGAGTCCCTGAGCACTGCCAACATTTCCC TTTCTCCAGTTGCACTATTCTGAGAAGGGNCGAATCTGCAGATATCCATCACACTGGCGG CCGCTCGACTCTAGATTGCNGCCGCGGTCATAGCTGTTTCCCTGACAGATCCCCGGGTGG CATCCCTGTGACCCCTCCCCAGTGCCTNTCCTGGCCCNTGNAGTTGCCACCTCAGTGCCC ACCCACCCTTGTTCCTAATAAAATAAAGNTGCATCATTTTGGCCTGACTAGGCGTCCTTC TTTAATATTATGGGGTGGAGGGGGGTGGGTATTGAACAGGGGCCAATTTTGGGAAAACAA CCGGTAGGGCCTGGGGGGGTCATT |
| Restriction Sites | Please inquire |
| ACCN | NM_015869 |
| Insert Size | 1600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015869.4, NP_056953.2 |
| RefSeq Size | 1820 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 5468 |
| UniProt ID | P37231 |
| Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
| Protein Pathways | Huntington's disease, Pathways in cancer, PPAR signaling pathway, Thyroid cancer |
| Gene Summary | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) encodes the longest isoform (2). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Human Receptor Activation By Aroclor 1260, a Polychlorinated Biphenyl Mixture
,Wahlang, B;Falkner, KC;Clair, HB;Al-Eryani, L;Prough, RA;States, JC;Coslo, DM;Omiecinski, CJ;Cave, MC;,
Toxicol. Sci.
,PubMed ID 24812009
[PPARG]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212502 | PPARG (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2 |
CN¥ 5,640.00 |
|
| RC212502L1 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2, Myc-DDK-tagged |
CN¥ 8,040.00 |
|
| RC212502L2 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2, mGFP tagged |
CN¥ 8,040.00 |
|
| RC212502L3 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2, Myc-DDK-tagged |
CN¥ 6,460.00 |
|
| RC212502L4 | Lenti ORF clone of Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2, mGFP tagged |
CN¥ 8,040.00 |
|
| RG212502 | PPARG (tGFP-tagged) - Human peroxisome proliferator-activated receptor gamma (PPARG), transcript variant 2 |
CN¥ 7,240.00 |
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