Zonula occludens protein 3 (TJP3) (NM_014428) Human Untagged Clone

CAT#: SC304103

TJP3 (untagged)-Human tight junction protein 3 (zona occludens 3) (TJP3)



  "NM_014428" in other vectors (4)

CNY 18,430.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (4)
TJP3 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ZO-3; ZO3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_014428 edited
ATGAACCTGTGTGGCCTCATGCCCATCTTCCCCGCTCCCCTCGACCAGGTGGCTGACATG
GAGGAGCTGACCATCTGGGAACAGCACACGGCCACACTGTCCAAGGACCCCCACCGGGGC
TTTGGCATTGCGATCTCTGGAGGCCGAGACCGGCCCGGTGGATCCATGGTTGTATCTGAC
GTGGTACCTGGAGGGCCGGCGGAGGGCAGGCTACAGACAGGCGACCACATTGTCATGGTG
AACGGGGTTTCCATGGAGAATGCCACCTCCGCGTTTGCCATTCAGATACTCAAGACCTGC
ACCAAGATGGCCAACATCACAGTGAAACGTCCCCGGAGGATCCACCTGCCCGCCACCAAA
GCCAGCCCCTCCAGCCCAGGGCGCCAGGACTCGGATGAAGACGATGGGCCCCAGCGGGTG
GAGGAGGTGGACCAGGGCCGGGGCTATGACGGCGACTCATCCAGTGGCTCCGGCCGCTCC
TGGGACGAGCGCTCCCGCCGGCCGAGGCCTGGTCGCCGGGGCCGGGCCGGCAGCCATGGG
CGTAGGAGCCCAGGTGGTGGCTCTGAGGCCAACGGGCTGGCCCTGGTGTCCGGCTTTAAG
CGGCTGCCACGGCAGGACGTGCAGATGAAGCCTGTGAAGTCAGTGCTGGTGAAGAGGAGA
GACAGCGAAGAGTTTGGCGTCAAGCTGGGCAGTCAGATCTTCATCAAGCACATTACAGAT
TCAGGCCTGGCTGCCCGGCACCGTGGGCTGCAGGAAGGAGATCTCATTCTACAGATCAAC
GGGGTGTCTAGCCAGAACCTGTCACTGAACGACACCCGGCGACTGATTGAGAAGTCAGAA
GGGAAGCTAAGCCTGCTGGTGCTGAGAGATCGTGGGCAGTTCCTGGTGAACATTCCGCCT
GCTGTCAGTGACAGCGACAGCTCGCCATTGGAGGAAGGCGTGACCATGGCTGATGAGATG
TCCTCTCCCCCTGCAGACATCTCGGACCTCGCCTCGGAGCTATCGCAGGCACCACCATCC
CACATCCCACCACCACCCCGGCATGCTCAGCGGAGCCCCGAGGCCAGCCAGACCGACTCT
CCCGTGGAGAGTCCCCGGCTTCGGCGGGAAAGTTCAGTAGATTCCAGAACCATCTCGGAA
CCAGATGAGCAACGGTCAGAGTTGCCCAGGGAAAGCAGCTATGACATCTACAGAGTGCCC
AGCAGTCAGAGCATGGAGGATCGTGGGTACAGCCCCGACACGCGTGTGGTCCGCTTCCTC
AAGGGCAAGAGCATCGGGCTGCGGCTGGCAGGGGGCAATGACGTGGGCATCTTCGTGTCC
GGGGTGCAGGCGGGCAGCCCGGCCGACGGGCAGGGCATCCAGGAGGGAGATCAGATTCTG
CAGGTGAATGACGTGCCATTCCAGAACCTGACACGGGAGGAGGCAGTGCAGTTCCTGCTG
GGGCTGCCACCAGGCGAGGAGATGGAGCTGGTGACGCAGCGGAAGCAGGACATTTTCTGG
AAAATGGTGCAGTCCCGCGTGGGTGACTCCTTCTACATCCGCACTCACTTTGAGCTGGAG
CCCAGTCCGCCGTCTGGCCTGGGCTTCACCCGTGGCGACGTCTTCCACGTGCTGGACACG
CTGCACCCCGGCCCCGGGCAGAGCCACGCACGAGGAGGCCACTGGCTGGCGGTGCGCATG
GGTCGTGACCTGCGGGAGCAAGAGCGGGGCATCATTCCCAACCAGAGCAGGGCGGAGCAG
CTGGCCAGCCTGGAAGCTGCCCAGAGGGCCGTGGGAGTCGGGCCCGGCTCCTCCGCGGGC
TCCAATGCTCGGGCCGAGTTCTGGCGGCTGCGGGGTCTGCGTCGAGGAGCCAAGAAGACC
ACTCAGCGGAGCCGTGAGGACCTCTCAGCTCTGACCCGACAGGGCCGCTACCCGCCCTAC
GAACGAGTGGTGTTGCGAGAAGCCAGTTTCAAGCGCCCGGTAGTGATCCTGGGACCCGTG
GCCGACATTGCTATGCAGAAGTTGACTGCTGAGATGCCTGACCAGTTTGAAATCGCAGAG
ACTGTGTCCAGGACCGACAGCCCCTCCAAGATCATCAAACTAGACACCGTGCGGGTGATT
GCAGAAAAAGACAAGCATGCGCTCCTGGATGTGACCCCCTCCGCCATCGAGCGCCTCAAC
TATGTGCAGTACTACCCCATTGTGGTCTTCTTCATCCCCGAGAGCCGGCCGGCCCTCAAG
GCACTGCGCCAGTGGCTGGCGCCTGCCTCCCGCCGCAGCACCCGTCGCCTCTACGCACAA
GCCCAGAAGCTGCGAAAACACAGCAGCCACCTCTTCACAGCCACCATCCCTCTGAATGGC
ACGAGTGACACCTGGTACCAGGAGCTCAAGGCCATCATTCGAGAGCAGCAGACGCGGCCC
ATCTGGACGGCGGAAGATCAGCTGGATGGCTCCTTGGAGGACAACCTAGACCTCCCTCAC
CACGGCCTGGCCGACAGCTCCGCTGACCTCAGCTGCGACAGCCGCGTTAACAGCGACTAC
GAGACGGACGGCGAGGGCGGCGCGTACACGGATGGCGAGGGCTACACAGACGGCGAGGGG
GGGCCCTACACGGATGTGGATGATGAGCCCCCGGCTCCAGCCCTGGCCCGGTCCTCGGAG
CCCGTGCAGGCAGATGAGTCCCAGAGCCCGAGGGATCGTGGGAGAATCTCGGCTCATCAG
GGGGCCCAGGTGGACAGCCGCCACCCCCAGGGACAGTGGCGACAGGACAGCATGCGAACC
TATGAACGGGAAGCCCTGAAGAAAAAGTTTACGCGAGTCCATGATGCGGAGTCCTCCGAT
GAAGACGGCTATGACTGGGGTCCGGCCACTGACCTGTGA
Restriction Sites Please inquire     
ACCN NM_014428
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to contain 3 SNPs compared with NM_014428.1.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014428.1, NP_055243.1
RefSeq Size 2859 bp
RefSeq ORF 2859 bp
Locus ID 27134
Protein Pathways Tight junction
Gene Summary The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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