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DKK2 (NM_014421) Human Untagged Clone
CAT＃: SC304099
DKK2 (untagged)-Human dickkopf homolog 2 (Xenopus laevis) (DKK2)
CNY 3,600.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DKK-2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_014421 edited
CTCCCTCCCTTTGCATTCCCACCCCTCCGGGCTTTGCGTCTTCCTGGGGACCCCCTCGCC GGGAGATGGCCGCGTTGATGCGGAGCAAGGATTCGTCCTGCTGCCTGCTCCTACTGGCCG CGGTGCTGATGGTGGAGAGCTCACAGATCGGCAGTTCGCGGGCCAAACTCAACTCCATCA AGTCCTCTCTGGGCGGGGAGACGCCTGGTCAGGCCGCCAATCGATCTGCGGGCATGTACC AAGGACTGGCATTCGGCGGCAGTAAGAAGGGCAAAAACCTGGGGCAGGCCTACCCTTGTA GCAGTGATAAGGAGTGTGAAGTTGGGAGGTATTGCCACAGTCCCCACCAAGGATCATCGG CCTGCATGGTGTGTCGGAGAAAAAAGAAGCGCTGCCACCGAGATGGCATGTGCTGCCCCA GTACCCGCTGCAATAATGGCATCTGTATCCCAGTTACTGAAAGCATCTTAACCCCTCACA TCCCGGCTCTGGATGGTACTCGGCACAGAGATCGAAACCACGGTCATTACTCAAACCATG ACTTGGGATGGCAGAATCTAGGAAGACCACACACTAAGATGTCACATATAAAAGGGCATG AAGGAGACCCCTGCCTACGATCATCAGACTGCATTGAAGGGTTTTGCTGTGCTCGTCATT TCTGGACCAAAATCTGCAAACCAGTGCTCCATCAGGGGGAAGTCTGTACCAAACAACGCA AGAAGGGTTCTCATGGGCTGGAAATTTTCCAGCGTTGCGACTGTGCGAAGGGCCTGTCTT GCAAAGTATGGAAAGATGCCACCTACTCCTCCAAAGCCAGACTCCATGTGTGTCAGAAAA TTTGATCACCATTGAGGAACATCATCAATTGCAGACTGTGAAGTTGTGTATTTAATGCAT TATAGCATGGTGGAAAATAAGGTTCAGATGCAGAAGAATGG >OriGene 5' read for NM_014421 unedited
GTGGAGTTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGCCCTTCTCCCTC CCTTTGCATTCCCACCCCTCCGGGCTTTGCGTCTTCCTGGGGACCCCCTCGCCGGGAGAT GGCCGCGTTGATGCGGAGCAAGGATTCGTCCTGCTGCCTGCTCCTACTGGCCGCGGTGCT GATGGTGGAGAGCTCACAGATCGGCAGTTCGCGGGCCAAACTCAACTCCATCAAGTCCTC TCTGGGCGGGGAGACGCCTGGTCAGGCCGCCAATCGATCTGCGGGCATGTACCAAGGACT GGCATTCGGCGGCAGTAAGAAGGGCAAAAACCTGGGGCAGGCCTACCCTTGTAGCAGTGA TAAGGAGTGTGAAGTTGGGAGGTATTGCCACAGTCCCCACCAAGGATCATCGGCCTGCAT GGTGTGTCGGAGAAAAAAGAAGCGCTGCCACCGAGATGGCATGTGCTGCCCCAGTACCCG CTGCAATAATGGCATCTGTATCCCAGTTACTGAAAGCATCTTAACCCCTCACATCCCGGC TCTGGATGGTACTCGGCACAGAGATCGAAACCACGGTCATTACTCAAACCATGACTTGGG ATGGCAGAATCTAGGAAGACCACACACTAAGATGTCACATATAAAAGGGCATGAAGGAGA CCCCTGCCTACGATCATCAGACTGCATTGAAGGGTTTTGCTGTGCTCGTCATTTCTGGAC CAAAATCTGCAAACCAGTGCTCCATCAGGGGGAAGTCTGTACCAAACAACGCAAGAAGGG TTCTCATGGGCTGGAAATTTTCCAGCGTTGCGACTGTGCGAAGGGCCTGTCTTGCAAGTA TGGAAAGATGCCACCTACTCCTCCCAAGCCAGACTCCATGTGTGTCAGAAAATTTG >OriGene 3' read for NM_014421 unedited
NGGCCCTGGNGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATGCC ACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTT GATATCGGTACCAGATCTGAATTCGCCCTTCCCATTCTTCTGCATCTGAACCTTATTTTC CACCATGCTATAATGCATTAAATACACAACTTCACAGTCTGCAATTGATGATGTTCCTCA ATGGTGATCAAATTTTCTGACACACATGGAGTCTGGCTTTGGAGGAGTAGGTGGCATCTT TCCATACTTTGCAAGACAGGCCCTTCGCACAGTCGCAACGCTGGAAAATTTCCAGCCCAT GAGAACCCTTCTTGCGTTGTTTGGTACAGACTTCCCCCTGATGGAGCACTGGTTTGCAGA TTTTGGTCCAGAAATGACGAGCACAGCAAAACCCTTCAATGCAGTCTGATGATCGTAGGC AGGGGTCTCCTTCATGCCCTTTTATATGTGACATCTTAGTGTGTGGTCTTCCTAGATTCT GCCATCCCAAGTCATGGTTTGAGTAATGACCGTGGTTTCGATCTCTGTGCCGAGTACCAT CCAGAGCCGGGATGTGAGGGGTTAAGATGCTTTCAGTAACTGGGATACAGATGCCATTAT TGCAGCGGGTACTGGGGCAGCACATGCCATCTCGGTGGCAGCGCTTCTTTTTTCTCCGAC ACACCATGCAGGCCGATGATCCTTGGTGGGGACTGTGGCAATACCTCCCAACTTCACACT CCTTATCACTGCTACAAGGGTAGGCCTGCCCCANGTTTTTGCCCTTCTTACTGCCGCCGA ATGCCAGTCCTTGGTACATGCCCGCAGATCGATTGGCGGCCTGACCAGGCGTCTCCCCGC CG |
| Restriction Sites | Please inquire |
| ACCN | NM_014421 |
| Insert Size | 950 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014421.2, NP_055236.1 |
| RefSeq Size | 3659 bp |
| RefSeq ORF | 780 bp |
| Locus ID | 27123 |
| UniProt ID | Q9UBU2 |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway |
| Protein Pathways | Wnt signaling pathway |
| Gene Summary | This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Genistein downregulates onco-miR-1260b and inhibits Wnt-signalling in renal cancer cells
,H Hirata, K Ueno, K Nakajima, Z L Tabatabai, Y Hinoda, + et al.,
British Journal of Cancer doi:10.1038/bjc.2013.173
,PubMed ID 23591200
[Dkk2]
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Epigenetic silencing of DKK2 and Wnt signal pathway components in human ovarian carcinoma
,Jing Zhu, Shu Zhang, Liying Gu, and Wen Di,
Carcinogenesis, Dec 2012; 33: 2334 - 2343.
[DKK2]
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Wnt Antagonist Gene DKK2 Is Epigenetically Silenced and Inhibits Renal Cancer Progression through Apoptotic and Cell Cycle Pathways
,Hiroshi Hirata, Yuji Hinoda, Koichi Nakajima, Ken Kawamoto, Nobuyuki Kikuno, Kazumori Kawakami, Soichiro Yamamura, Koji Ueno, Shahana Majid, Sharanjot Saini, Nobuhisa Ishii, and Rajvir Dahiya,
Clin. Cancer Res., Sep 2009; 15: 5678 - 5687
[DKK2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224432 | DKK2 (Myc-DDK-tagged)-Human dickkopf homolog 2 (Xenopus laevis) (DKK2) |
CNY 3,600.00 |
|
| RC224432L1 | Lenti ORF clone of Human dickkopf homolog 2 (Xenopus laevis) (DKK2), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC224432L2 | Lenti ORF clone of Human dickkopf homolog 2 (Xenopus laevis) (DKK2), mGFP tagged |
CNY 5,890.00 |
|
| RC224432L3 | Lenti ORF clone of Human dickkopf homolog 2 (Xenopus laevis) (DKK2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224432L4 | Lenti ORF clone of Human dickkopf homolog 2 (Xenopus laevis) (DKK2), mGFP tagged |
CNY 6,000.00 |
|
| RG224432 | DKK2 (tGFP-tagged) - Human dickkopf homolog 2 (Xenopus laevis) (DKK2) |
CNY 5,200.00 |
