LAMB4 (NM_007356) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303920 representing NM_007356. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAATTTCAACTGACCCTTTTTTTGCACCTTGGGTGGCTCAGTTACTCAAAAGCTCAAGATGACTGC AACAGGGGTGCCTGTCATCCCACCACTGGTGATCTCCTGGTGGGCAGGAACACGCAGCTTATGGCTTCT TCTACCTGTGGGCTGAGCAGAGCCCAGAAATACTGCATCCTCAGTTACCTGGAGGGGGAACAAAAATGC TTCATCTGTGACTCTAGATTTCCATATGATCCGTATGACCAACCCAACAGCCACACCATTGAGAATGTC ATTGTAAGTTTTGAACCAGACAGAGAAAAGAAATGGTGGCAATCTGAAAATGGTCTTGATCATGTCAGC ATCAGACTGGACTTAGAGGCATTATTTCGGTTCAGCCACCTTATCCTGACCTTTAAGACTTTTCGGCCT GCTGCAATGTTAGTTGAACGTTCCACAGACTATGGACACAACTGGAAAGTGTTCAAATATTTTGCAAAA GACTGTGCCACTTCCTTTCCTAACATCACATCTGGCCAGGCCCAGGGAGTGGGAGACATTGTTTGTGAC TCCAAATACTCGGATATTGAACCCTCAACAGGTGGAGAGGTTGTTTTAAAAGTTTTGGATCCCAGTTTT GAAATTGAAAACCCTTATAGCCCCTACATCCAAGACCTTGTGACATTGACAAACCTGAGGATAAACTTT ACCAAGCTCCACACCCTTGGGGATGCTTTGCTTGGAAGGAGGCAAAATGATTCCCTTGATAAATACTAC TATGCTCTGTACGAGATGATTGTTCGGGGAAGCTGCTTTTGCAATGGCCATGCTAGCGAATGTCGCCCT ATGCAGAAGATGCGGGGAGATGTTTTCAGCCCTCCTGGAATGGTTCACGGTCAGTGTGTGTGTCAGCAC AATACAGATGGTCCGAACTGTGAGAGATGCAAGGACTTCTTCCAGGATGCTCCTTGGAGGCCAGCTGCA GACCTCCAGGACAACGCTTGCAGATCGTGCAGCTGTAATAGCCACTCCAGCCGCTGTCACTTTGACATG ACTACGTACCTGGCAAGCGGTGGCCTCAGCGGGGGCGTGTGTGAAGACTGCCAGCACAACACTGAGGGG CAGCACTGCGACCGCTGCAGACCCCTCTTCTACAGGGACCCGCTCAAGACCATCTCAGATCCCTACGCG TGCATTCCTTGTGAATGTGACCCCGATGGGACCATATCTGGTGGCATTTGTGTGAGCCACTCTGATCCT GCCTTAGGGTCTGTGGCCGGCCAGTGCCTTTGTAAAGAGAACGTGGAAGGAGCCAAATGCGACCAGTGC AAACCCAACCACTACGGACTAAGCGCCACCGACCCCCTGGGCTGCCAGCCCTGCGACTGTAACCCCCTT GGGAGTCTGCCATTCTTGACCTGTGATGTGGATACAGGCCAATGCTTGTGCCTGTCATATGTCACCGGA GCACACTGCGAAGAATGCACTGTTGGATACTGGGGCCTGGGAAATCATCTCCATGGGTGTTCTCCCTGT GACTGTGATATTGGAGGTGCTTATTCTAACGTGTGCTCACCCAAGAATGGGCAGTGTGAATGCCGCCCA CATGTCACTGGCCGTAGCTGCTCTGAACCAGCCCCTGGCTACTTCTTTGCTCCTTTGAATTTCTATCTC TACGAGGCAGAGGAAGCCACAACACTCCAAGGACTGGCGCCTTTGGGCTCGGAGACGTTTGGCCAGAGT CCTGCTGTTCACGTTGTTTTAGGAGAGCCAGTTCCTGGGAACCCTGTTACATGGACTGGACCTGGATTT GCCAGGGTTCTCCCTGGGGCTGGCTTGAGATTTGCTGTCAACAACATTCCCTTTCCTGTGGACTTCACC ATTGCCATTCACTATGAAACCCAGTCTGCAGCTGACTGGACTGTCCAGATTGTGGTGAACCCCCCTGGA GGGAGTGAGCACTGCATACCCAAGACTCTACAGTCAAAGCCTCAGTCTTTTGCCTTACCAGCGGCTACG AGAATCATGCTGCTTCCCACACCCATCTGTTTAGAACCAGATGTACAATATTCCATAGATGTCTATTTT TCTCAGCCTTTGCAAGGAGAGTCCCACGCTCATTCACATGTCCTGGTGGACTCTCTTGGCCTTATTCCC CAAATCAATTCATTGGAGAATTTCTGCAGCAAGCAGGACTTAGATGAGTATCAGCTTCACAACTGTGTT GAAATTGCCTCAGCAATGGGACCTCAAGTGCTCCCGGGTGCCTGTGAAAGGCTGATCATCAGCATGTCT GCCAAGCTGCATGATGGGGCTGTGGCCTGCAAGTGTCACCCCCAGGGCTCAGTCGGATCCAGCTGCAGC CGACTTGGAGGCCAGTGCCAGTGTAAACCTCTTGTGGTCGGGCGCTGCTGTGACAGGTGCTCAACTGGA AGCTATGATTTGGGGCATCACGGCTGTCACCCATGTCACTGCCATCCTCAAGGATCAAAGGACACTGTA TGTGACCAAGTAACAGGACAGTGCCCCTGCCATGGAGAGGTGTCTGGCCGCCGCTGTGATCGCTGCCTG GCAGGCTACTTTGGATTTCCCAGCTGCCACCCTTGCCCTTGTAATAGGTTTGCTGAACTTTGTGATCCT GAGACAGGGTCATGCTTCAATTGTGGAGGCTTTACAACTGGCAGAAACTGTGAAAGGTGTATTGATGGT TACTATGGAAATCCTTCTTCAGGACAGCCCTGTCGTCCTTGCCTGTGTCCAGATGATCCCTCAAGCAAT CAGTATTTTGCCCATTCCTGTTATCAGAATCTGTGGAGCTCAGATGTAATCTGCAATTGTCTTCAAGGT TATACGGGTACTCAGTGTGGAGAATGCTCTACTGGTTTCTATGGAAATCCAAGAATTTCAGGAGCACCT TGCCAACCATGTGCCTGCAACAACAACATAGATGTAACCGATCCAGAGTCCTGCAGCCGGGTAACAGGG GAGTGCCTTCGATGTTTGCACAACACTCAGGGCGCAAACTGCCAGCTCTGCAAACCAGGTCACTATGGA TCAGCCCTCAATCAGACCTGCAGAAGATGCTCCTGCCATGCTTCCGGCGTGAGTCCCATGGAGTGTCCC CCTGGTGGGGGAGCTTGCCTCTGTGACCCTGTCACTGGTGCATGTCCTTGTCTGCCGAATGTCACAGGC CTGGCCTGTGACCGTTGTGCTGATGGATACTGGAATCTGGTCCCTGGCAGAGGATGTCAGTCATGTGAC TGTGACCCTAGGACCTCTCAAAGTAGCCACTGTGACCAGCTTACAGGCCAGTGTCCGTGTAAATTAGGT TACGGCGGGAAACGTTGCAGTGAGTGCCAGGAAAATTATTATGGTGATCCACCTGGGCGATGCATTCCA TGTGATTGTAACAGGGCAGGTACCCAGAAGCCCATCTGTGATCCAGACACAGGCATGTGCCGCTGCCGG GAGGGTGTCAGCGGCCAGAGATGTGATCGCTGTGCCCGGGGACACAGCCAGGAATTCCCTACTTGTCTT CAATGTCACTTGTGCTTTGATCAGTGGGACCACACCATTTCTTCCCTCTCCAAAGCGGTGCAAGGGTTA ATGAGACTGGCTGCTAACATGGAAGATAAAAGAGAGACCCTGCCTGTCTGTGAGGCAGACTTCAAAGAC CTCAGAGGGAACGTGTCTGAAATAGAAAGGATTTTGAAACATCCTGTTTTCCCATCTGGGAAATTCTTA AAAGTCAAGGATTATCATGACTCTGTTAGAAGACAAATCATGCAGCTAAATGAACAACTGAAAGCAGTG TATGAATTTCAAGATCTGAAAGATACAATAGAAAGAGCAAAGAATGAAGCAGACCTCTTACTTGAAGAC CTTCAGGAAGAAATTGATTTGCAATCCAGTGTCCTTAATGCAAGCATTGCGGACTCCTCAGAAAACATC AAGAAATATTATCACATATCATCATCTGCTGAAAAGAAAATTAATGAAACTAGTTCCACCATTAATACC TCTGCAAATACAAGGAATGACTTACTTACCATCTTAGATACACTAACCTCAAAAGGAAACTTGTCATTG GAAAGATTAAAGCAGATTAAGATACCAGATATCCAAATATTGAATGAAAAGGTGTGCGGAGATCCAGGA AATGTGCCATGTGTGCCCTTGCCCTGTGGCGGTGCTCTCTGCACGGGCCGGAAGGGGCACAGGAAGTGT AGGGGTCCCGGCTGTCACGGCTCCCTGACCCTCTCAACGAATGCCCTCCAAAAAGCCCAGGAAGCAAAA TCCATTATTCGTAATTTGGACAAACAGGTTCGTGGGTTGAAAAATCAGATCGAAAGTATAAGTGAACAG GCAGAAGTCTCCAAAAACAATGCCTTACAGCTGAGGGAAAAACTGGGAAATATAAGAAACCAAAGTGAC TCTGAAGAAGAAAACATCAATCTTTTCATCAAAAAAGTGAAAAACTTTTTGTTAGAGGAAAACGTGCCT CCAGAAGACATCGAGAAGGTTGCGAATGGTGTGCTTGACATTCACCTACCAATTCCATCCCAAAATCTA ACCGATGAACTTGTCAAAATACAGAAACATATGCAACTCTGTGAGGATTACAGGACAGATGAAAACAGG TTAAATGAAGAAGCAGATGGAGCCCAAAAGCTTTTGGTGAAGGCCAAAGCAGCTGAGAAAGCAGCAAAT ATTCTATTAAATCTTGACAAAACATTGAACCAGTTACAACAAGCTCAAATCACTCAAGGACGGGCAAAC TCTACCATTACACAGCTGACTGCCAATATAACAAAAATAAAAAAGAATGTGCTGCAGGCTGAAAATCAA ACCAGGGAAATGAAGAGTGAGCTGGAGTTAGCAAAGCAGCGATCAGGGCTGGAGGATGGACTTTCCCTG CTGCAGACCAAGTTGCAAAGGCATCAAGACCACGCTGTCAATGCGAAAGTTCAGGCTGAATCTGCCCAA CACCAGGCTGGGAGTCTTGAGAAGGAATTTGTTGAGCTGAAAAAACAATATGCTATTCTCCAACGTAAG ACAAGCACTACAGGACTAACAAAGGAGACATTAGGAAAAGTTAAACAGCTAAAAGATGCGGCAGAAAAA TTGGCTGGAGATACAGAGGCCAAGATAAGAAGAATAACAGATTTAGAAAGGAAAATCCAAGATTTGAAT CTAAGTAGACAAGCAAAAGCTGATCAACTGAGAATATTGGAAGATCAAGTTGTTGCCATTAAAAATGAA ATTGTTGAACAAGAAAAAAAATATGCTAGGTGCTATAGCTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_007356
Insert Size	5286 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007356.2
RefSeq Size	5858 bp
RefSeq ORF	5286 bp
Locus ID	22798
UniProt ID	A4D0S4
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
MW	193.5 kDa
Gene Summary	Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.