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SARDH (NM_007101) Human Untagged Clone
CAT＃: SC303875
SARDH (untagged)-Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BPR-2; DMGDHL1; SAR; SARD; SDH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303875 representing NM_007101.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCTCACTGAGCCGAGCCCTACGTGTGGCTGCTGCCCACCCTCGCCAGAGCCCTACCCGGGGCATG GGGCCATGCAACCTGTCCAGCGCAGCTGGCCCCACAGCCGAGAAGAGTGTGCCATATCAGCGGACCCTG AAGGAGGGACAGGGCACCTCGGTGGTGGCCCAAGGCCCAAGCCGGCCCCTGCCCAGCACGGCCAACGTG GTGGTCATTGGTGGAGGCAGCTTGGGCTGCCAGACCCTGTACCACCTGGCCAAGCTGGGCATGAGTGGG GCGGTGCTGCTGGAGCGGGAGCGGCTGACCTCCGGGACCACCTGGCACACGGCAGGCCTGCTGTGGCAG CTGCGGCCCAGTGACGTGGAGGTGGAGCTTCTGGCCCACACTCGGCGGGTGGTGAGCCGGGAGCTGGAG GAGGAGACGGGACTACACACGGGCTGGATCCAGAATGGGGGCCTCTTCATCGCGTCCAACCGGCAGCGC CTGGACGAGTACAAGAGGCTCATGTCGCTGGGCAAGGCGTATGGTGTGGAATCCCATGTGCTGAGCCCG GCAGAGACCAAGACTCTGTACCCGCTGATGAATGTGGACGACCTCTACGGGACCCTGTATGTGCCGCAC GACGGTACCATGGACCCCGCTGGCACCTGTACCACCCTCGCCAGGGCAGCTTCTGCCCGAGGAGCACAG GTCATTGAGAACTGCCCAGTGACCGGCATTCGTGTGTGGACGGATGATTTTGGGGTGCGGCGGGTCGCG GGTGTGGAGACTCAGCATGGTTCCATCCAGACACCCTGCGTGGTCAACTGTGCAGGAGTGTGGGCAAGT GCTGTGGGCCGGATGGCTGGAGTCAAGGTCCCGCTGGTGGCCATGCACCATGCCTATGTCGTCACCGAG CGCATCGAGGGGATTCAGAACATGCCCAATGTCCGTGATCATGATGCCTCTGTCTACCTCCGCCTCCAA GGGGATGCCTTGTCTGTGGGTGGCTATGAGGCCAACCCCATCTTTTGGGAGGAGGTGTCAGACAAGTTT GCCTTCGGCCTCTTTGACCTGGACTGGGAGGTGTTCACCCAGCACATTGAAGGCGCCATCAACAGGGTC CCCGTGCTGGAGAAGACAGGAATCAAGTCCACGGTCTGCGGCCCTGAATCCTTCACGCCCGACCACAAG CCCCTGATGGGGGAGGCACCTGAGCTCCGAGGGTTCTTCCTGGGCTGTGGCTTCAACAGCGCAGGAATG ATGCTGGGTGGTGGCTGTGGGCAGGAGCTGGCCCACTGGATCATCCATGGGCGCCCGGAGAAGGACATG CATGGCTATGACATCAGGCGCTTCCATCACTCGCTCACGGACCACCCCCGCTGGATCCGAGAGCGAAGC CATGAGTCCTACGCCAAGAACTACTCCGTCGTCTTCCCCCACGATGAGCCGCTGGCCGGGCGCAACATG AGGAGAGACCCGCTGCACGAGGAACTCCTTGGACAAGGCTGCGTGTTCCAGGAGCGGCATGGCTGGGAG CGACCGGGATGGTTTCATCCCCGAGGCCCAGCTCCGGTCCTCGAGTACGACTACTACGGGGCTTACGGG AGCCGCGCGCACGAGGACTACGCCTACCGCAGGCTGCTGGCAGACGAGTACACCTTCGCCTTCCCGCCC CACCACGACACGATCAAGAAGGAGTGCCTGGCCTGCAGAGGGGCCGCCGCTGTGTTTGACATGTCCTAC TTCGGGAAGTTCTACCTGGTGGGGCTGGATGCAAGGAAGGCTGCCGACTGGCTCTTCTCCGCAGATGTC AGCCGACCCCCAGGCTCCACCGTGTACACGTGCATGCTCAACCACCGTGGGGGCACCGAGAGTGACCTG ACTGTCAGCCGCCTGGCACCCAGCCACCAGGCCTCCCCGCTGGCCCCCGCCTTTGAAGGGGACGGTTAC TACCTGGCCATGGGCGGGGCCGTGGCCCAGCACAACTGGTCCCACATCACCACCGTGCTGCAGGACCAG AAGTCCCAGTGCCAGCTCATCGACAGCTCCGAGGACCTGGGTATGATCAGTATCCAGGGCCCAGCCAGC CGAGCCATTTTGCAGGAGGTGCTGGACGCAGACCTGAGCAACGAGGCCTTCCCGTTCTCCACCCACAAG CTACTGAGAGCCGCAGGGCACCTGGTCCGAGCCATGCGGCTGTCCTTTGTGGGGGAGCTGGGCTGGGAG CTGCACATTCCAAAGGCGTCCTGCGTGCCTGTGTACCGGGCTGTGATGGCCGCGGGTGCCAAGCACGGC CTCATCAACGCAGGGTACCGCGCCATCGACTCCCTGAGCATTGAGAAAGGCTACCGGCACTGGCACGCG GACCTGCGGCCAGACGACAGCCCCCTGGAGGCAGGCCTGGCCTTCACCTGCAAGCTCAAGTCGCCGGTG CCCTTCCTGGGGAGGGAGGCCCTGGAGCAGCAGCGGGCCGCAGGCCTCCGCCGGCGCCTGGTGTGCTTC ACCATGGAGGACAAAGTACCCATGTTTGGCCTGGAGGCCATCTGGAGGAACGGCCAAGTGGTGGGCCAT GTCCGGAGGGCTGACTTTGGGTTCGCCATCGACAAGACCATCGCCTACGGTTACATCCATGACCCCAGC GGTGGGCCGGTCTCGCTGGACTTTGTGAAGAGCGGGGACTATGCCCTGGAGAGAATGGGGGTGACCTAT GGTGCCCAGGCTCACCTGAAGTCGCCCTTCGACCCCAACAACAAGAGGGTGAAGGGAATCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_007101 |
| Insert Size | 2757 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007101.3 |
| RefSeq Size | 3398 bp |
| RefSeq ORF | 2757 bp |
| Locus ID | 1757 |
| UniProt ID | Q9UL12 |
| Protein Pathways | Glycine, serine and threonine metabolism, Metabolic pathways |
| MW | 101 kDa |
| Gene Summary | This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same isoform. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219173 | SARDH (Myc-DDK-tagged)-Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,720.00 |
|
| RC219173L3 | Lenti ORF clone of Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219173L4 | Lenti ORF clone of Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG219173 | SARDH (tGFP-tagged) - Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 8,320.00 |
