SPTBN2 (NM_006946) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GTRAP41; SCA5; SCAR14
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303857 representing NM_006946. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCAGCACGCTGTCACCCACAGACTTTGACAGCTTGGAAATCCAGGGCCAGTACAGTGACATCAAC AACCGCTGGGACCTTCCTGACTCGGACTGGGACAATGACAGCAGCTCGGCCCGCCTCTTTGAGAGGTCT CGCATTAAGGCTCTGGCAGATGAACGAGAAGCTGTGCAGAAGAAAACCTTCACCAAGTGGGTAAACTCG CACCTGGCCCGGGTCACGTGCCGGGTGGGGGACCTGTACAGCGACCTCCGGGACGGACGCAACCTGCTG AGGCTCCTCGAGGTGCTCTCGGGAGAGATACTGCCAAAGCCTACAAAGGGCCGCATGCGGATCCACTGC CTGGAGAACGTGGACAAGGCACTGCAGTTCCTCAAGGAGCAGAAAGTGCACTTGGAAAACATGGGCTCC CATGACATTGTGGACGGAAACCACCGACTGACCCTTGGGCTGGTCTGGACCATCATCCTTCGATTCCAG ATCCAAGACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGATGCCCTGCTTCTG TGGTGCCAGATGAAGACTGCAGGTTATCCCAACGTCAATGTACACAACTTCACCACCAGCTGGAGAGAT GGACTAGCTTTCAACGCCATCGTGCATAAACACCGGCCAGACCTGCTGGATTTTGAGTCTCTGAAGAAG TGTAATGCACACTATAATCTGCAGAATGCATTCAATCTGGCTGAAAAGGAACTGGGACTTACCAAGCTG CTGGATCCCGAAGACGTGAATGTGGACCAGCCAGATGAGAAGTCAATCATTACCTATGTGGCTACTTAC TACCATTACTTCTCCAAGATGAAGGCCCTGGCCGTGGAAGGCAAGAGAATTGGCAAGGTGCTGGACCAT GCCATGGAGGCAGAGCGCCTGGTGGAGAAATACGAGTCCCTGGCCTCGGAGCTGCTGCAGTGGATCGAG CAAACGATCGTGACCCTCAATGACCGGCAGTTGGCCAACTCCCTTAGCGGGGTCCAGAACCAGCTGCAG TCCTTCAACTCCTACCGCACCGTGGAGAAGCCGCCCAAGTTTACCGAGAAAGGGAACTTGGAAGTGCTG CTCTTCACCATCCAGAGCAAGCTTCGGGCCAACAACCAGAAGGTCTACACGCCCCGCGAGGGCCGGCTC ATCTCGGACATCAACAAGGCTTGGGAGCGGCTGGAGAAGGCGGAGCACGAGCGTGAGCTGGCCCTGCGC ACCGAGCTCATCCGCCAGGAGAAGCTGGAGCAGCTGGCCGCCCGCTTCGACCGCAAGGCTGCCATGCGG GAGACCTGGCTCAGCGAGAACCAGCGCCTCGTGTCCCAGGACAACTTTGGGCTGGAGCTGGCAGCTGTC GAGGCAGCAGTACGGAAGCACGAAGCCATTGAGACGGACATCGTGGCCTACAGCGGCCGGGTGCAGGCA GTGGACGCCGTGGCTGCAGAGCTGGCCGCCGAGCGCTACCACGACATCAAGCGCATCGCCGCTCGGCAG CACAACGTGGCACGGCTCTGGGACTTCTTGCGGCAGATGGTGGCCGCCCGGCGGGAGCGGCTCCTCCTC AACCTGGAGCTGCAGAAGGTGTTCCAGGACCTGCTCTACCTCATGGACTGGATGGAAGAGATGAAGGGC CGGCTGCAGTCTCAGGACCTGGGCAGGCACCTAGCAGGAGTGGAGGACCTGCTGCAGCTGCACGAGCTG GTGGAGGCAGACATCGCCGTGCAGGCCGAGAGGGTGCGGGCCGTCAGCGCCTCTGCCCTGCGCTTCTGC AACCCAGGGAAAGAGTATAGACCTTGCGACCCGCAGCTGGTGTCGGAGCGGGTGGCCAAGCTAGAGCAG AGCTATGAGGCACTGTGCGAGTTGGCAGCGGCGCGGCGGGCCCGGCTGGAGGAATCACGGCGGCTCTGG CGTTTCCTCTGGGAGGTGGGTGAAGCTGAGGCCTGGGTGCGGGAGCAGCAGCACCTCCTGGCCTCAGCC GACACGGGCCGAGACCTGACCGGTGCCCTCCGCCTGCTCAACAAGCACACAGCCCTGCGGGGCGAGATG AGCGGCCGGCTGGGGCCCCTGAAGCTCACCCTGGAGCAGGGCCAGCAGTTGGTGGCCGAGGGTCACCCT GGGGCAAGCCAGGCCTCTGCCCGTGCAGCTGAACTCCAAGCCCAGTGGGAGCGGCTAGAGGCCCTGGCC GAGGAGCGTGCCCAGCGGCTGGCCCAAGCCGCCAGCCTCTACCAGTTCCAGGCCGATGCAAACGACATG GAGGCCTGGTTGGTTGACGCACTGCGCCTGGTGTCCAGCCCCGAGCTGGGGCACGACGAGTTCTCCACG CAGGCTCTAGCCAGGCAGCATCGGGCCCTGGAGGAGGAGATTCGAAGCCACCGGCCAACCCTGGACGCC TTGAGGGAACAGGCAGCAGCCCTGCCCCCCACACTGAGCCGCACGCCCGAGGTGCAGGGCCGGGTGCCC ACCCTGGAGCGGCACTACGAGGAGCTGCAGGCCCGGGCAGGCGAGCGAGCGCGGGCCTTGGAGGCAGCC CTGGCGCTCTACACCATGCTCAGCGAGGCCGGGGCCTGTGGACTCTGGGTGGAGGAGAAGGAGCAGTGG CTCAACGGGCTGGCCCTGCCTGAACGCCTGGAGGACCTGGAGGTCGTGCAGCAGAGGTTCGAGACCCTG GAGCCTGAAATGAACACCCTTGCAGCACAAATCACCGCGGTGAATGACATTGCCGAGCAGTTACTGAAG GCCAACCCCCCAGGCAAAGACCGCATTGTCAACACCCAGGAGCAGCTCAACCACAGGTGGCAGCAGTTT CGGCGTCTGGCAGACGGCAAGAAGGCAGCTCTCACCTCAGCCCTGAGCATCCAGAACTACCACTTAGAG TGCACGGAGACCCAGGCCTGGATGAGAGAGAAGACCAAAGTCATCGAGTCCACCCAGGGCCTAGGCAAC GATCTGGCTGGGGTGCTGGCCCTGCAGCGCAAGCTGGCCGGCACGGAGCGGGACCTGGAGGCCATCGCC GCCCGGGTGGGCGAACTGACTCGAGAGGCAAATGCCCTGGCTGCCGGCCATCCCGCTCAGGCAGTGGCC ATCAACGCCCGGCTGAGAGAGGTGCAGACCGGCTGGGAGGACCTCAGGGCCACCATGCGGCGTCGAGAA GAGTCGCTGGGGGAGGCGCGGCGGCTGCAGGACTTCTTGCGCAGCTTGGATGACTTCCAGGCCTGGCTA GGCCGCACTCAGACTGCTGTGGCCTCTGAAGAAGGGCCGGCCACCCTGCCTGAGGCAGAGGCCCTCCTG GCCCAACATGCAGCCCTGCGGGGAGAGGTGGAGCGGGCCCAGAGCGAGTATAGCCGGCTGCGAGCCCTG GGCGAGGAGGTGACCCGGGACCAGGCTGACCCCCAGTGCCTCTTCCTACGACAGCGACTGGAGGCCCTG GGAACTGGCTGGGAGGAGCTGGGCCGAATGTGGGAGAGCCGGCAAGGTCGCCTGGCCCAGGCCCACGGC TTCCAGGGATTCCTGCGGGATGCTCGTCAGGCTGAGGGCGTGCTCAGCAGCCAGGAATATGTTCTGTCT CACACGGAGATGCCAGGGACACTCCAGGCTGCTGATGCTGCCATTAAAAAACTGGAGGACTTCATGAGC ACCATGGACGCCAATGGGGAACGGATCCACGGGCTCCTGGAGGCTGGCCGCCAGCTGGTATCTGAAGGC AACATCCACGCCGACAAGATTCGGGAAAAGGCAGACTCCATTGAGAGGAGGCACAAGAAGAATCAAGAC GCAGCGCAGCAATTTCTGGGCCGTCTTCGGGACAACCGGGAGCAGCAGCATTTCCTGCAAGATTGTCAC GAGCTGAAGCTCTGGATCGACGAGAAGATGCTGACAGCCCAGGACGTGTCCTATGACGAGGCCCGCAAC CTGCATACTAAGTGGCAGAAGCACCAGGCATTCATGGCCGAGCTGGCTGCCAACAAAGACTGGCTGGAC AAGGTGGACAAGGAAGGGCGAGAGCTCACCCTTGAGAAGCCAGAGCTGAAAGCCCTGGTGTCGGAGAAG CTGAGAGACCTGCACAGGCGCTGGGACGAGCTGGAGACCACCACCCAAGCCAAGGCCCGCAGCCTCTTT GATGCCAACCGAGCTGAGCTGTTTGCCCAGAGCTGCTGTGCCCTGGAGAGCTGGCTGGAGAGCCTGCAG GCCCAGCTGCACTCGGATGACTACGGCAAGGACCTCACCAGCGTCAACATCCTGCTCAAGAAGCAGCAG ATGCTGGAATGGGAGATGGCTGTGAGAGAGAAGGAGGTGGAGGCAATCCAGGCCCAGGCCAAAGCACTG GCCCAGGAGGACCAGGGTGCAGGGGAGGTGGAGAGAACCTCGAGGGCCGTGGAGGAGAAGTTCAGGGCC TTGTGCCAGCCCATGCGGGAACGCTGCCGGCGCCTGCAGGCTTCTCGCGAGCAGCACCAGTTCCACCGC GATGTGGAAGATGAGATTTTGTGGGTGACAGAGCGGCTGCCCATGGCCAGCTCCATGGAGCATGGCAAG GACCTGCCCAGCGTCCAGCTTCTCATGAAGAAAAACCAGACCCTGCAGAAAGAGATTCAGGGCCATGAG CCCCGGATCGCGGACCTGAGGGAGCGGCAGCGTGCTCTAGGTGCAGCAGCAGCAGGTCCAGAGCTGGCT GAGCTGCAGGAAATGTGGAAACGCCTGGGCCACGAGCTGGAACTTCGAGGGAAGCGACTGGAGGATGCC CTGCGAGCCCAGCAGTTCTACCGCGATGCCGCCGAGGCGGAGGCCTGGATGGGCGAGCAGGAATTACAC ATGATGGGCCAGGAGAAGGCCAAGGATGAGCTGAGTGCCCAGGCAGAGGTGAAGAAGCACCAGGTGCTG GAGCAAGCCCTGGCCGACTACGCGCAGACCATCCACCAGCTGGCGGCCAGCAGCCAGGACATGATTGAC CACGAGCACCCAGAGAGCACTCGGATATCCATCCGCCAAGCCCAGGTGGACAAGCTGTATGCCGGCCTG AAGGAGCTGGCTGGAGAGCGGCGGGAGCGCCTGCAGGAGCACCTCCGGCTGTGCCAGCTCCGCCGCGAG CTGGATGACCTGGAACAGTGGATCCAGGAGCGCGAGGTGGTGGCGGCCTCCCACGAGCTGGGCCAGGAC TACGAGCATGTGACTATGCTCCGAGACAAATTCCGAGAGTTCTCCCGGGACACAAGCACCATCGGTCAG GAGCGCGTAGATAGCGCCAATGCGCTGGCCAATGGGCTCATTGCTGGGGGCCATGCTGCACGGGCCACC GTGGCCGAGTGGAAGGACAGTCTCAACGAGGCCTGGGCTGACCTGCTTGAGCTGCTGGACACACGGGGT CAGGTGCTGGCCGCGGCGTACGAGCTGCAGCGCTTCCTGCACGGGGCACGCCAAGCCCTGGCGCGGGTG CAGCACAAGCAGCAGCAGCTTCCGGACGGGACTGGCCGCGACCTCAACGCTGCCGAGGCCCTGCAGCGC CGACACTGTGCCTACGAGCATGACATTCAGGCCCTCAGCCCCCAGGTCCAGCAGGTGCAGGACGACGGC CACCGGCTCCAGAAGGCCTACGCTGGAGACAAGGCTGAGGAGATCGGCCGCCACATGCAGGCCGTGGCC GAGGCCTGGGCCCAGCTTCAGGGAAGCTCTGCCGCCCGCCGGCAGCTGCTGCTGGACACCACAGACAAG TTCCGCTTCTTCAAGGCTGTCCGGGAACTGATGCTCTGGATGGATGAGGTCAACCTGCAGATGGATGCC CAGGAGCGTCCCCGGGATGTGTCCTCCGCGGATCTAGTCATCAAGAACCAGCAAGGCATCAAGGCAGAG ATAGAGGCCCGGGCAGACCGCTTCTCCTCCTGCATCGACATGGGGAAGGAGCTGCTGGCCAGGAGCCAC TATGCGGCCGAGGAGATCTCAGAGAAGCTGTCTCAGCTGCAGGCACGGCGCCAGGAGACAGCTGAGAAG TGGCAGGAGAAGATGGACTGGCTTCAGCTGGTTTTGGAGGTGCTTGTGTTTGGAAGAGATGCAGGGATG GCAGAGGCCTGGCTCTGCAGCCAGGAGCCACTGGTGCGCAGCGCTGAGCTGGGTTGCACGGTCGACGAA GTTGAGAGCCTCATCAAGCGGCACGAGGCCTTCCAGAAGTCAGCAGTGGCCTGGGAGGAGCGATTCTGT GCGCTGGAGAAGCTTACTGCGCTAGAGGAGCGGGAGAAGGAGCGAAAGAGAAAGAGGGAGGAGGAGGAG CGGCGGAAACAGCCGCCTGCTCCCGAACCCACAGCCAGTGTGCCTCCAGGGGACCTGGTGGGCGGCCAG ACAGCTTCTGACACCACCTGGGACGGAACCCAGCCACGGCCACCACCATCCACACAAGCACCCAGTGTT AATGGAGTCTGCACAGATGGAGAGCCCTCACAGCCCCTGCTGGGACAACAGAGACTTGAGCACAGCAGC TTCCCCGAAGGGCCGGGACCTGGCTCAGGGGACGAAGCCAATGGGCCCCGGGGAGAGAGGCAGACCCGG ACTCGGGGCCCGGCCCCATCTGCAATGCCCCAGAGCAGGTCTACCGAGTCAGCCCATGCTGCCACCCTG CCGCCTCGAGGCCCAGAGCCATCTGCCCAGGAGCAGATGGAGGGGATGCTGTGCCGCAAGCAGGAGATG GAGGCCTTCGGGAAGAAGGCTGCCAACAGGTCCTGGCAGAACGTGTACTGTGTCCTGCGGCGTGGGAGC CTCGGCTTTTACAAGGATGCCAAGGCAGCCAGCGCGGGAGTGCCATACCACGGAGAAGTGCCTGTCAGC CTGGCCAGGGCCCAGGGCAGCGTCGCCTTTGATTACCGAAAGCGCAAACATGTCTTCAAGCTGGGCTTA CAGGATGGAAAAGAATATTTATTCCAGGCCAAGGATGAGGCAGAGATGAGCTCGTGGCTACGGGTGGTG AATGCAGCCATTGCCACAGCGTCTTCTGCCTCTGGAGAGCCTGAAGAGCCGGTGGTGCCCAGCACCACC CGGGGCATGACCCGGGCCATGACCATGCCCCCAGTGTCACCCGTCGGGGCTGAGGGGCCTGTTGTGCTC CGCAGCAAAGACGGCAGAGAACGAGAGCGAGAAAAACGCTTCAGCTTCTTTAAGAAGAACAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_006946
Insert Size	7173 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006946.2
RefSeq Size	7884 bp
RefSeq ORF	7173 bp
Locus ID	6712
UniProt ID	O15020
MW	271.3 kDa
Gene Summary	Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]

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