SPTBN2 (NM_006946) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GTRAP41; SCA5; SCAR14
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC222257 representing NM_006946 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCACGCTGTCACCCACAGACTTTGACAGCTTGGAAATCCAGGGCCAGTACAGTGACATCAACA ACCGCTGGGACCTTCCTGACTCGGACTGGGACAATGACAGCAGCTCGGCCCGCCTCTTTGAGAGGTCTCG CATTAAGGCTCTGGCAGATGAACGAGAAGCTGTGCAGAAGAAAACCTTCACCAAGTGGGTAAACTCGCAC CTGGCCCGGGTCACGTGCCGGGTGGGGGACCTGTACAGCGACCTCCGGGACGGACGCAACCTGCTGAGGC TCCTCGAGGTGCTCTCGGGAGAGATACTGCCAAAGCCTACAAAGGGCCGCATGCGGATCCACTGCCTGGA GAACGTGGACAAGGCACTGCAGTTCCTCAAGGAGCAGAAAGTGCACTTGGAAAACATGGGCTCCCATGAC ATTGTGGACGGAAACCACCGACTGACCCTTGGGCTGGTCTGGACCATCATCCTTCGATTCCAGATCCAAG ACATCAGTGTGGAGACAGAAGACAACAAGGAGAAGAAGTCAGCCAAGGATGCCCTGCTTCTGTGGTGCCA GATGAAGACTGCAGGTTATCCCAACGTCAATGTACACAACTTCACCACCAGCTGGAGAGATGGACTAGCT TTCAACGCCATCGTGCATAAACACCGGCCAGACCTGCTGGATTTTGAGTCTCTGAAGAAGTGTAATGCAC ACTATAATCTGCAGAATGCATTCAATCTGGCTGAAAAGGAACTGGGACTTACCAAGCTGCTGGATCCCGA AGACGTGAATGTGGACCAGCCAGATGAGAAGTCAATCATTACCTATGTGGCTACTTACTACCATTACTTC TCCAAGATGAAGGCCCTGGCCGTGGAAGGCAAGAGAATTGGCAAGGTGCTGGACCATGCCATGGAGGCAG AGCGCCTGGTGGAGAAATACGAGTCCCTGGCCTCGGAGCTGCTGCAGTGGATCGAGCAAACGATCGTGAC CCTCAATGACCGGCAGTTGGCCAACTCCCTTAGCGGGGTCCAGAACCAGCTGCAGTCCTTCAACTCCTAC CGCACCGTGGAGAAGCCGCCCAAGTTTACCGAGAAAGGGAACTTGGAAGTGCTGCTCTTCACCATCCAGA GCAAGCTTCGGGCCAACAACCAGAAGGTCTACACGCCCCGCGAGGGCCGGCTCATCTCGGACATCAACAA GGCTTGGGAGCGGCTGGAGAAGGCGGAGCACGAGCGTGAGCTGGCCCTGCGCACCGAGCTCATCCGCCAG GAGAAGCTGGAGCAGCTGGCCGCCCGCTTCGACCGCAAGGCTGCCATGCGGGAGACCTGGCTCAGCGAGA ACCAGCGCCTCGTGTCCCAGGACAACTTTGGGCTGGAGCTGGCAGCTGTCGAGGCAGCAGTACGGAAGCA CGAAGCCATTGAGACGGACATCGTGGCCTACAGCGGCCGGGTGCAGGCAGTGGACGCCGTGGCTGCAGAG CTGGCCGCCGAGCGCTACCACGACATCAAGCGCATCGCCGCTCGGCAGCACAACGTGGCACGGCTCTGGG ACTTCTTGCGGCAGATGGTGGCCGCCCGGCGGGAGCGGCTCCTCCTCAACCTGGAGCTGCAGAAGGTGTT CCAGGACCTGCTCTACCTCATGGACTGGATGGAAGAGATGAAGGGCCGGCTGCAGTCTCAGGACCTGGGC AGGCACCTAGCAGGAGTGGAGGACCTGCTGCAGCTGCACGAGCTGGTGGAGGCAGACATCGCCGTGCAGG CCGAGAGGGTGCGGGCCGTCAGCGCCTCTGCCCTGCGCTTCTGCAACCCAGGGAAAGAGTATAGACCTTG CGACCCGCAGCTGGTGTCGGAGCGGGTGGCCAAGCTAGAGCAGAGCTATGAGGCACTGTGCGAGTTGGCA GCGGCGCGGCGGGCCCGGCTGGAGGAATCACGGCGGCTCTGGCGTTTCCTCTGGGAGGTGGGTGAAGCTG AGGCCTGGGTGCGGGAGCAGCAGCACCTCCTGGCCTCAGCCGACACGGGCCGAGACCTGACCGGTGCCCT CCGCCTGCTCAACAAGCACACAGCCCTGCGGGGCGAGATGAGCGGCCGGCTGGGGCCCCTGAAGCTCACC CTGGAGCAGGGCCAGCAGTTGGTGGCCGAGGGTCACCCTGGGGCAAGCCAGGCCTCTGCCCGTGCAGCTG AACTCCAAGCCCAGTGGGAGCGGCTAGAGGCCCTGGCCGAGGAGCGTGCCCAGCGGCTGGCCCAAGCCGC CAGCCTCTACCAGTTCCAGGCCGATGCAAACGACATGGAGGCCTGGTTGGTTGACGCACTGCGCCTGGTG TCCAGCCCCGAGCTGGGGCACGACGAGTTCTCCACGCAGGCTCTAGCCAGGCAGCATCGGGCCCTGGAGG AGGAGATTCGAAGCCACCGGCCAACCCTGGACGCCTTGAGGGAACAGGCAGCAGCCCTGCCCCCCACACT GAGCCGCACGCCCGAGGTGCAGGGCCGGGTGCCCACCCTGGAGCGGCACTACGAGGAGCTGCAGGCCCGG GCAGGCGAGCGAGCGCGGGCCTTGGAGGCAGCCCTGGCGCTCTACACCATGCTCAGCGAGGCCGGGGCCT GTGGACTCTGGGTGGAGGAGAAGGAGCAGTGGCTCAACGGGCTGGCCCTGCCTGAACGCCTGGAGGACCT GGAGGTCGTGCAGCAGAGGTTCGAGACCCTGGAGCCTGAAATGAACACCCTTGCAGCACAAATCACCGCG GTGAATGACATTGCCGAGCAGTTACTGAAGGCCAACCCCCCAGGCAAAGACCGCATTGTCAACACCCAGG AGCAGCTCAACCACAGGTGGCAGCAGTTTCGGCGTCTGGCAGACGGCAAGAAGGCAGCTCTCACCTCAGC CCTGAGCATCCAGAACTACCACTTAGAGTGCACGGAGACCCAGGCCTGGATGAGAGAGAAGACCAAAGTC ATCGAGTCCACCCAGGGCCTAGGCAACGATCTGGCTGGGGTGCTGGCCCTGCAGCGCAAGCTGGCCGGCA CGGAGCGGGACCTGGAGGCCATCGCCGCCCGGGTGGGCGAACTGACTCGAGAGGCAAATGCCCTGGCTGC CGGCCATCCCGCTCAGGCAGTGGCCATCAACGCCCGGCTGAGAGAGGTGCAGACCGGCTGGGAGGACCTC AGGGCCACCATGCGGCGTCGAGAAGAGTCGCTGGGGGAGGCGCGGCGGCTGCAGGACTTCTTGCGCAGCT TGGATGACTTCCAGGCCTGGCTAGGCCGCACTCAGACTGCTGTGGCCTCTGAAGAAGGGCCGGCCACCCT GCCTGAGGCAGAGGCCCTCCTGGCCCAACATGCAGCCCTGCGGGGAGAGGTGGAGCGGGCCCAGAGCGAG TATAGCCGGCTGCGAGCCCTGGGCGAGGAGGTGACCCGGGACCAGGCTGACCCCCAGTGCCTCTTCCTAC GACAGCGACTGGAGGCCCTGGGAACTGGCTGGGAGGAGCTGGGCCGAATGTGGGAGAGCCGGCAAGGTCG CCTGGCCCAGGCCCACGGCTTCCAGGGATTCCTGCGGGATGCTCGTCAGGCTGAGGGCGTGCTCAGCAGC CAGGAATATGTTCTGTCTCACACGGAGATGCCAGGGACACTCCAGGCTGCTGATGCTGCCATTAAAAAAC TGGAGGACTTCATGAGCACCATGGACGCCAATGGGGAACGGATCCACGGGCTCCTGGAGGCTGGCCGCCA GCTGGTATCTGAAGGCAACATCCACGCCGACAAGATTCGGGAAAAGGCAGACTCCATTGAGAGGAGGCAC AAGAAGAATCAAGACGCAGCGCAGCAATTTCTGGGCCGTCTTCGGGACAACCGGGAGCAGCAGCATTTCC TGCAAGATTGTCACGAGCTGAAGCTCTGGATCGACGAGAAGATGCTGACAGCCCAGGACGTGTCCTATGA CGAGGCCCGCAACCTGCATACTAAGTGGCAGAAGCACCAGGCATTCATGGCCGAGCTGGCTGCCAACAAA GACTGGCTGGACAAGGTGGACAAGGAAGGGCGAGAGCTCACCCTTGAGAAGCCAGAGCTGAAAGCCCTGG TGTCGGAGAAGCTGAGAGACCTGCACAGGCGCTGGGACGAGCTGGAGACCACCACCCAAGCCAAGGCCCG CAGCCTCTTTGATGCCAACCGAGCTGAGCTGTTTGCCCAGAGCTGCTGTGCCCTGGAGAGCTGGCTGGAG AGCCTGCAGGCCCAGCTGCACTCGGATGACTACGGCAAGGACCTCACCAGCGTCAACATCCTGCTCAAGA AGCAGCAGATGCTGGAATGGGAGATGGCTGTGAGAGAGAAGGAGGTGGAGGCAATCCAGGCCCAGGCCAA AGCACTGGCCCAGGAGGACCAGGGTGCAGGGGAGGTGGAGAGAACCTCGAGGGCCGTGGAGGAGAAGTTC AGGGCCTTGTGCCAGCCCATGCGGGAACGCTGCCGGCGCCTGCAGGCTTCTCGCGAGCAGCACCAGTTCC ACCGCGATGTGGAAGATGAGATTTTGTGGGTGACAGAGCGGCTGCCCATGGCCAGCTCCATGGAGCATGG CAAGGACCTGCCCAGCGTCCAGCTTCTCATGAAGAAAAACCAGACCCTGCAGAAAGAGATTCAGGGCCAT GAGCCCCGGATCGCGGACCTGAGGGAGCGGCAGCGTGCTCTAGGTGCAGCAGCAGCAGGTCCAGAGCTGG CTGAGCTGCAGGAAATGTGGAAACGCCTGGGCCACGAGCTGGAACTTCGAGGGAAGCGACTGGAGGATGC CCTGCGAGCCCAGCAGTTCTACCGCGATGCCGCCGAGGCGGAGGCCTGGATGGGCGAGCAGGAATTACAC ATGATGGGCCAGGAGAAGGCCAAGGATGAGCTGAGTGCCCAGGCAGAGGTGAAGAAGCACCAGGTGCTGG AGCAAGCCCTGGCCGACTACGCGCAGACCATCCACCAGCTGGCGGCCAGCAGCCAGGACATGATTGACCA CGAGCACCCAGAGAGCACTCGGATATCCATCCGCCAAGCCCAGGTGGACAAGCTGTATGCCGGCCTGAAG GAGCTGGCTGGAGAGCGGCGGGAGCGCCTGCAGGAGCACCTCCGGCTGTGCCAGCTCCGCCGCGAGCTGG ATGACCTGGAACAGTGGATCCAGGAGCGCGAGGTGGTGGCGGCCTCCCACGAGCTGGGCCAGGACTACGA GCATGTGACTATGCTCCGAGACAAATTCCGAGAGTTCTCCCGGGACACAAGCACCATCGGTCAGGAGCGC GTAGATAGCGCCAATGCGCTGGCCAATGGGCTCATTGCTGGGGGCCATGCTGCACGGGCCACCGTGGCCG AGTGGAAGGACAGTCTCAACGAGGCCTGGGCTGACCTGCTTGAGCTGCTGGACACACGGGGTCAGGTGCT GGCCGCGGCGTACGAGCTGCAGCGCTTCCTGCACGGGGCACGCCAAGCCCTGGCGCGGGTGCAGCACAAG CAGCAGCAGCTTCCGGACGGGACTGGCCGCGACCTCAACGCTGCCGAGGCCCTGCAGCGCCGACACTGTG CCTACGAGCATGACATTCAGGCCCTCAGCCCCCAGGTCCAGCAGGTGCAGGACGACGGCCACCGGCTCCA GAAGGCCTACGCTGGAGACAAGGCTGAGGAGATCGGCCGCCACATGCAGGCCGTGGCCGAGGCCTGGGCC CAGCTTCAGGGAAGCTCTGCCGCCCGCCGGCAGCTGCTGCTGGACACCACAGACAAGTTCCGCTTCTTCA AGGCTGTCCGGGAACTGATGCTCTGGATGGATGAGGTCAACCTGCAGATGGATGCCCAGGAGCGTCCCCG GGATGTGTCCTCCGCGGATCTAGTCATCAAGAACCAGCAAGGCATCAAGGCAGAGATAGAGGCCCGGGCA GACCGCTTCTCCTCCTGCATCGACATGGGGAAGGAGCTGCTGGCCAGGAGCCACTATGCGGCCGAGGAGA TCTCAGAGAAGCTGTCTCAGCTGCAGGCACGGCGCCAGGAGACAGCTGAGAAGTGGCAGGAGAAGATGGA CTGGCTTCAGCTGGTTTTGGAGGTGCTTGTGTTTGGAAGAGATGCAGGGATGGCAGAGGCCTGGCTCTGC AGCCAGGAGCCACTGGTGCGCAGCGCTGAGCTGGGTTGCACGGTCGACGAAGTTGAGAGCCTCATCAAGC GGCACGAGGCCTTCCAGAAGTCAGCAGTGGCCTGGGAGGAGCGATTCTGTGCGCTGGAGAAGCTTACTGC GCTAGAGGAGCGGGAGAAGGAGCGAAAGAGAAAGAGGGAGGAGGAGGAGCGGCGGAAACAGCCGCCTGCT CCCGAACCCACAGCCAGTGTGCCTCCAGGGGACCTGGTGGGCGGCCAGACAGCTTCTGACACCACCTGGG ACGGAACCCAGCCACGGCCACCACCATCCACACAAGCACCCAGTGTTAATGGAGTCTGCACAGATGGAGA GCCCTCACAGCCCCTGCTGGGACAACAGAGACTTGAGCACAGCAGCTTCCCCGAAGGGCCGGGACCTGGC TCAGGGGACGAAGCCAATGGGCCCCGGGGAGAGAGGCAGACCCGGACTCGGGGCCCGGCCCCATCTGCAA TGCCCCAGAGCAGGTCTACCGAGTCAGCCCATGCTGCCACCCTGCCGCCTCGAGGCCCAGAGCCATCTGC CCAGGAGCAGATGGAGGGGATGCTGTGCCGCAAGCAGGAGATGGAGGCCTTCGGGAAGAAGGCTGCCAAC AGGTCCTGGCAGAACGTGTACTGTGTCCTGCGGCGTGGGAGCCTCGGCTTTTACAAGGATGCCAAGGCAG CCAGCGCGGGAGTGCCATACCACGGAGAAGTGCCTGTCAGCCTGGCCAGGGCCCAGGGCAGCGTCGCCTT TGATTACCGAAAGCGCAAACATGTCTTCAAGCTGGGCTTACAGGATGGAAAAGAATATTTATTCCAGGCC AAGGATGAGGCAGAGATGAGCTCGTGGCTACGGGTGGTGAATGCAGCCATTGCCACAGCGTCTTCTGCCT CTGGAGAGCCTGAAGAGCCGGTGGTGCCCAGCACCACCCGGGGCATGACCCGGGCCATGACCATGCCCCC AGTGTCACCCGTCGGGGCTGAGGGGCCTGTTGTGCTCCGCAGCAAAGACGGCAGAGAACGAGAGCGAGAA AAACGCTTCAGCTTCTTTAAGAAGAACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC222257 representing NM_006946 Red=Cloning site Green=Tags(s) MSSTLSPTDFDSLEIQGQYSDINNRWDLPDSDWDNDSSSARLFERSRIKALADEREAVQKKTFTKWVNSH LARVTCRVGDLYSDLRDGRNLLRLLEVLSGEILPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHD IVDGNHRLTLGLVWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLA FNAIVHKHRPDLLDFESLKKCNAHYNLQNAFNLAEKELGLTKLLDPEDVNVDQPDEKSIITYVATYYHYF SKMKALAVEGKRIGKVLDHAMEAERLVEKYESLASELLQWIEQTIVTLNDRQLANSLSGVQNQLQSFNSY RTVEKPPKFTEKGNLEVLLFTIQSKLRANNQKVYTPREGRLISDINKAWERLEKAEHERELALRTELIRQ EKLEQLAARFDRKAAMRETWLSENQRLVSQDNFGLELAAVEAAVRKHEAIETDIVAYSGRVQAVDAVAAE LAAERYHDIKRIAARQHNVARLWDFLRQMVAARRERLLLNLELQKVFQDLLYLMDWMEEMKGRLQSQDLG RHLAGVEDLLQLHELVEADIAVQAERVRAVSASALRFCNPGKEYRPCDPQLVSERVAKLEQSYEALCELA AARRARLEESRRLWRFLWEVGEAEAWVREQQHLLASADTGRDLTGALRLLNKHTALRGEMSGRLGPLKLT LEQGQQLVAEGHPGASQASARAAELQAQWERLEALAEERAQRLAQAASLYQFQADANDMEAWLVDALRLV SSPELGHDEFSTQALARQHRALEEEIRSHRPTLDALREQAAALPPTLSRTPEVQGRVPTLERHYEELQAR AGERARALEAALALYTMLSEAGACGLWVEEKEQWLNGLALPERLEDLEVVQQRFETLEPEMNTLAAQITA VNDIAEQLLKANPPGKDRIVNTQEQLNHRWQQFRRLADGKKAALTSALSIQNYHLECTETQAWMREKTKV IESTQGLGNDLAGVLALQRKLAGTERDLEAIAARVGELTREANALAAGHPAQAVAINARLREVQTGWEDL RATMRRREESLGEARRLQDFLRSLDDFQAWLGRTQTAVASEEGPATLPEAEALLAQHAALRGEVERAQSE YSRLRALGEEVTRDQADPQCLFLRQRLEALGTGWEELGRMWESRQGRLAQAHGFQGFLRDARQAEGVLSS QEYVLSHTEMPGTLQAADAAIKKLEDFMSTMDANGERIHGLLEAGRQLVSEGNIHADKIREKADSIERRH KKNQDAAQQFLGRLRDNREQQHFLQDCHELKLWIDEKMLTAQDVSYDEARNLHTKWQKHQAFMAELAANK DWLDKVDKEGRELTLEKPELKALVSEKLRDLHRRWDELETTTQAKARSLFDANRAELFAQSCCALESWLE SLQAQLHSDDYGKDLTSVNILLKKQQMLEWEMAVREKEVEAIQAQAKALAQEDQGAGEVERTSRAVEEKF RALCQPMRERCRRLQASREQHQFHRDVEDEILWVTERLPMASSMEHGKDLPSVQLLMKKNQTLQKEIQGH EPRIADLRERQRALGAAAAGPELAELQEMWKRLGHELELRGKRLEDALRAQQFYRDAAEAEAWMGEQELH MMGQEKAKDELSAQAEVKKHQVLEQALADYAQTIHQLAASSQDMIDHEHPESTRISIRQAQVDKLYAGLK ELAGERRERLQEHLRLCQLRRELDDLEQWIQEREVVAASHELGQDYEHVTMLRDKFREFSRDTSTIGQER VDSANALANGLIAGGHAARATVAEWKDSLNEAWADLLELLDTRGQVLAAAYELQRFLHGARQALARVQHK QQQLPDGTGRDLNAAEALQRRHCAYEHDIQALSPQVQQVQDDGHRLQKAYAGDKAEEIGRHMQAVAEAWA QLQGSSAARRQLLLDTTDKFRFFKAVRELMLWMDEVNLQMDAQERPRDVSSADLVIKNQQGIKAEIEARA DRFSSCIDMGKELLARSHYAAEEISEKLSQLQARRQETAEKWQEKMDWLQLVLEVLVFGRDAGMAEAWLC SQEPLVRSAELGCTVDEVESLIKRHEAFQKSAVAWEERFCALEKLTALEEREKERKRKREEEERRKQPPA PEPTASVPPGDLVGGQTASDTTWDGTQPRPPPSTQAPSVNGVCTDGEPSQPLLGQQRLEHSSFPEGPGPG SGDEANGPRGERQTRTRGPAPSAMPQSRSTESAHAATLPPRGPEPSAQEQMEGMLCRKQEMEAFGKKAAN RSWQNVYCVLRRGSLGFYKDAKAASAGVPYHGEVPVSLARAQGSVAFDYRKRKHVFKLGLQDGKEYLFQA KDEAEMSSWLRVVNAAIATASSASGEPEEPVVPSTTRGMTRAMTMPPVSPVGAEGPVVLRSKDGRERERE KRFSFFKKNK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_006946
ORF Size	7170 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006946.3
RefSeq Size	7864 bp
RefSeq ORF	7173 bp
Locus ID	6712
UniProt ID	O15020
MW	271.1 kDa
Gene Summary	Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. [provided by RefSeq, Dec 2009]

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