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valyl tRNA synthetase (VARS) (NM_006295) Human Untagged Clone
CAT＃: SC303765
VARS (untagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein
CNY 10,450.00
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | G7A; NDMSCA; VARS; VARS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303765 representing NM_006295.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCACCCTCTACGTCTCCCCTCACCCAGATGCCTTCCCCAGCCTCCGAGCCCTCATAGCCGCTCGC TATGGGGAGGCTGGGGAGGGTCCCGGATGGGGAGGAGCCCACCCCCGCATCTGTCTCCAGCCACCCCCG ACTAGCAGGACTCCCTTTCCCCCACCCCGCCTGCCGGCCCTGGAGCAGGGGCCCGGTGGGCTCTGGGTG TGGGGGGCCACGGCTGTGGCCCAGCTGCTGTGGCCAGCAGGCCTGGGGGGCCCAGGGGGCAGCCGGGCG GCTGTCCTTGTCCAACAGTGGGTCAGTTACGCCGACACGGAGTTAATACCAGCTGCCTGTGGAGCAACG CTGCCGGCCCTGGGACTCCGAAGCTCGGCCCAGGACCCCCAGGCTGTGCTGGGGGCCCTGGGCAGGGCC CTGAGCCCCTTGGAGGAGTGGCTTCGGCTGCACACCTACTTGGCCGGGGAGGCCCCCACTCTGGCTGAC CTGGCGGCTGTCACAGCCTTGCTGCTGCCTTTCCGATACGTCCTAGACCCACCTGCCCGCCGGATCTGG AATAATGTGACTCGCTGGTTTGTCACGTGTGTCCGGCAGCCAGAATTCCGAGCCGTGCTAGGAGAAGTG GTTCTATACTCAGGAGCCAGGCCTCTCTCTCATCAGCCAGGCCCCGAGGCTCCTGCCCTCCCAAAGACA GCTGCTCAGCTCAAGAAAGAGGCAAAGAAACGGGAGAAGCTAGAGAAATTCCAACAGAAGCAGAAGATC CAACAGCAGCAGCCACCTCCAGGGGAGAAGAAACCAAAACCAGAGAAGAGGGAGAAACGGGATCCTGGG GTCATTACCTATGACCTCCCAACCCCACCCGGGGAAAAGAAAGATGTCAGTGGCCCCATGCCCGACTCC TACAGCCCTCGGTATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCAGCAGGGCTTCTTCAAGCCAGAG TATGGGCGTCCTAATGTGTCAGCAGCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAAT GTGACAGGCTCCCTGCACCTGGGCCATGCACTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCAC CGCATGCGTGGGGAGACCACCCTGTGGAACCCTGGCTGTGACCATGCAGGTATTGCCACCCAGGTGGTG GTGGAGAAGAAGCTATGGCGTGAGCAGGGACTGAGCCGGCACCAGCTGGGCCGCGAGGCCTTTCTACAG GAAGTCTGGAAGTGGAAGGAGGAGAAAGGTGACCGGATTTACCACCAGTTGAAGAAGCTTGGCAGCTCC TTGGACTGGGATCGAGCCTGTTTCACCATGGACCCTAAACTCTCAGCAGCTGTGACAGAGGCCTTTGTC CGGCTTCACGAGGAAGGCATCATCTATCGCAGTACCCGCCTTGTTAACTGGTCCTGCACCCTCAACTCC GCCATCTCTGACATTGAGGTGGATAAGAAGGAGCTGACAGGTCGCACCCTGCTCTCCGTGCCTGGCTAC AAGGAGAAGGTGGAGTTCGGGGTCCTCGTGTCCTTTGCCTATAAGGTCCAAGGCTCAGATAGCGACGAG GAGGTGGTGGTGGCAACAACTCGGATCGAGACAATGCTGGGAGATGTGGCTGTAGCTGTGCACCCCAAA GATACCAGATACCAGCACCTGAAGGGGAAGAACGTGATCCACCCATTCCTGTCTCGGAGCCTTCCCATT GTCTTCGATGAATTTGTGGACATGGACTTTGGCACAGGTGCTGTGAAGATCACCCCCGCACATGACCAA AATGACTATGAAGTTGGGCAGCGGCACGGGCTGGAGGCCATCAGCATCATGGACTCCCGGGGGGCCCTC ATCAATGTGCCTCCGCCTTTCCTGGGCCTGCCCAGGTTTGAGGCCAGGAAAGCGGTGCTGGTGGCGCTG AAGGAGCGGGGACTGTTCCGTGGCATTGAGGACAACCCCATGGTGGTGCCACTTTGCAACCGGTCGAAG GACGTGGTAGAGCCTCTGCTGCGGCCGCAGTGGTACGTTCGCTGCGGGGAGATGGCCCAGGCTGCCAGC GCCGCTGTGACTCGGGGTGACCTCCGCATCCTGCCTGAGGCCCATCAGCGCACATGGCATGCCTGGATG GACAACATCCGGGAGTGGTGCATTTCCAGGCAGCTGTGGTGGGGCCATCGCATCCCAGCCTACTTTGTC ACTGTCAGTGACCCAGCGGTGCCCCCTGGGGAGGACCCTGATGGGCGGTACTGGGTGAGTGGACGCAAT GAGGCGGAGGCCCGGGAGAAGGCAGCCAAGGAGTTCGGAGTGTCCCCTGACAAGATCAGTCTCCAGCAA GATGAGGATGTATTGGATACCTGGTTCTCCTCTGGCCTCTTCCCCTTATCCATTTTGGGCTGGCCCAAC CAGTCAGAAGACCTGAGTGTGTTCTACCCCGGGACACTGCTGGAGACCGGTCATGACATCCTCTTCTTC TGGGTGGCCCGGATGGTCATGCTGGGCCTGAAGCTCACGGGCAGGCTGCCCTTTAGAGAGGTCTACCTC CATGCCATCGTGCGAGATGCTCACGGCCGGAAGATGAGCAAGTCTCTAGGCAATGTCATCGATCCCCTG GACGTCATCTATGGAATCTCCCTGCAGGGCCTCCACAACCAGCTGCTGAACAGCAACCTGGATCCCAGC GAGGTGGAGAAGGCCAAAGAAGGGCAGAAAGCTGACTTCCCAGCGGGGATTCCTGAATGTGGCACCGAT GCTCTCCGGTTTGGATTATGTGCCTACATGTCCCAGGGTCGTGACATCAACCTGGATGTGAACCGGATA CTGGGTTACCGCCACTTCTGCAACAAGCTCTGGAATGCCACCAAGTTTGCCCTTCGTGGCCTTGGGAAG GGTTTTGTGCCCTCACCCACCTCCCAGCCCGGAGGCCATGAGAGCCTGGTGGACCGCTGGATCCGCAGC CGCCTGACAGAGGCTGTGAGGCTCAGCAATCAAGGCTTCCAGGCCTACGACTTCCCGGCCGTCACCACT GCCCAGTACAGCTTCTGGCTCTATGAGCTCTGTGATGTCTACTTGGAGTGCCTGAAACCTGTACTGAAT GGGGTGGACCAGGTGGCAGCTGAGTGTGCCCGCCAGACCCTGTACACTTGCCTGGACGTTGGCCTGCGG CTGCTCTCACCCTTCATGCCCTTCGTGACGGAGGAGCTGTTCCAGAGGCTGCCCCGGAGGATGCCGCAA GCTCCCCCTAGCCTCTGTGTTACCCCCTACCCGGAGCCCTCAGAGTGCTCCTGGAAGGACCCCGAGGCA GAAGCCGCCCTTGAGCTGGCGCTAAGCATCACGCGAGCCGTGCGCTCCCTGCGGGCCGACTACAACCTC ACCCGGATCCGGCCTGACTGTTTCCTGGAAGTGGCGGATGAGGCCACGGGCGCCCTGGCATCGGCGGTG TCGGGCTACGTGCAGGCCCTGGCCAGCGCAGGTGTGGTGGCTGTTCTGGCCCTGGGGGCTCCCGCCCCC CAGGGTTGCGCTGTGGCTCTGGCTTCTGATCGCTGCTCCATCCACCTGCAGCTTCAGGGGCTGGTGGAC CCTGCACGGGAGCTGGGCAAGCTGCAAGCCAAGCGAGTTGAGGCCCAGCGGCAGGCCCAGCGTCTGCGG GAACGCCGTGCTGCCTCGGGCTATCCTGTCAAGGTGCCGCTCGAAGTCCAGGAGGCAGATGAAGCCAAG CTCCAACAGACAGAAGCAGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTATTCCAGAAGATGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_006295 |
| Insert Size | 3795 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006295.2 |
| RefSeq Size | 4308 bp |
| RefSeq ORF | 3795 bp |
| Locus ID | 7407 |
| UniProt ID | P26640 |
| Protein Families | Druggable Genome |
| Protein Pathways | Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis |
| MW | 140.5 kDa |
| Gene Summary | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201089 | VARS (Myc-DDK-tagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein |
CNY 8,800.00 |
|
| RC201089L1 | Lenti ORF clone of Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 11,200.00 |
|
| RC201089L2 | Lenti ORF clone of Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 12,730.00 |
|
| RC201089L3 | Lenti ORF clone of Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 12,730.00 |
|
| RC201089L4 | Lenti ORF clone of Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 11,200.00 |
|
| RG201089 | VARS (tGFP-tagged) - Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein |
CNY 11,880.00 |
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