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POLE (NM_006231) Human Untagged Clone
CAT＃: SC303754
POLE (untagged)-Human polymerase (DNA directed), epsilon (POLE)
CNY 44,080.00
货期*
4周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CRCS12; FILS; IMAGEI; POLE1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC303754 representing NM_006231.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTCTGAGGAGCGGCGGGCGGCGGCGCGCGGACCCAGGCGCGGATGGCGAGGCCAGCAGGGATGAT GGCGCCACTTCCTCAGTTTCGGCACTCAAGCGCCTGGAACGGAGTCAGTGGACGGATAAGATGGATTTG CGGTTTGGTTTTGAGCGGCTGAAGGAGCCTGGTGAGAAGACAGGCTGGCTCATTAACATGCATCCTACC GAGATTTTAGATGAAGATAAGCGCTTAGGCAGTGCAGTGGATTACTACTTTATTCAAGATGACGGAAGC AGATTTAAGGTGGCTTTGCCCTATAAACCGTATTTCTACATTGCGACCAGAAAGGGTTGTGAGCGAGAA GTTTCATCTTTTCTCTCCAAGAAGTTTCAGGGCAAAATTGCAAAAGTGGAGACTGTCCCCAAAGAGGAT CTGGACTTGCCAAATCACTTGGTGGGTTTGAAGCGAAATTACATCAGGCTGTCCTTCCACACTGTGGAG GATCTTGTCAAAGTGAGGAAGGAGATCTCCCCTGCCGTGAAGAAGAACAGGGAGCAGGATCACGCCAGC GACGCGTACACAGCTCTGCTTTCCAGTGTTCTGCAGAGGGGCGGTGTCATTACTGATGAAGAGGAAACC TCTAAGAAGATAGCTGACCAGTTGGACAACATTGTGGACATGCGCGAGTACGATGTTCCCTACCACATC CGCCTCTCCATTGACCTGAAGATCCACGTGGCTCATTGGTACAATGTCAGATACCGAGGAAATGCTTTT CCGGTAGAAATCACCCGCCGAGATGACCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATT GAGACGACCAAACTGCCCCTCAAGTTTCCTGATGCTGAGACAGACCAGATTATGATGATTTCCTACATG ATCGATGGCCAGGGCTACCTCATCACCAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTC ACCCCCAAGCCAGAATATGAAGGCCCCTTTTGTGTCTTCAATGAACCCGATGAGGCTCATCTGATCCAA AGGTGGTTTGAACACGTCCAGGAGACCAAACCCACCATCATGGTCACCTACAACGGGGACTTTTTTGAC TGGCCATTTGTGGAGGCCCGGGCAGCAGTCCACGGTCTGAGCATGCAGCAGGAGATAGGCTTCCAGAAG GACAGCCAGGGGGAGTACAAGGCGCCCCAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGAC AGTTACCTTCCTGTGGGCAGTCATAATCTCAAGGCGGCCGCCAAGGCCAAGCTAGGCTATGATCCCGTG GAGCTAGACCCGGAGGACATGTGCCGGATGGCCACGGAGCAGCCCCAGACTCTGGCCACGTATTCTGTG TCAGATGCTGTCGCCACTTACTACCTGTACATGAAGTACGTCCACCCATTCATCTTTGCTCTGTGCACC ATTATTCCCATGGAGCCCGACGAGGTGCTGCGGAAGGGCTCTGGCACTCTGTGTGAGGCCTTGCTGATG GTGCAGGCCTTCCACGCCAACATCATCTTCCCCAACAAGCAAGAGCAGGAGTTCAATAAGCTGACGGAC GACGGACACGTGCTGGACTCTGAGACCTACGTCGGGGGCCACGTGGAGGCCCTCGAGTCTGGGGTTTTC CGCAGCGATATCCCTTGCCGGTTTAGGATGAATCCTGCCGCCTTTGACTTCCTGCTGCAGCGGGTTGAG AAGACCTTGCGCCACGCCCTTGAGGAAGAGGAGAAAGTGCCTGTGGAGCAAGTCACCAACTTTGAAGAG GTGTGTGATGAGATTAAGAGCAAGCTTGCCTCCCTGAAGGACGTTCCCAGCCGCATCGAGTGTCCACTC ATCTACCACCTGGACGTGGGGGCCATGTACCCCAACATCATCCTGACCAACCGCCTGCAGCCCTCTGCC ATGGTGGACGAAGCCACCTGTGCTGCCTGTGACTTCAATAAGCCTGGAGCAAACTGCCAGCGGAAGATG GCCTGGCAGTGGAGGGGCGAGTTCATGCCAGCCAGTCGCAGCGAATACCATCGGATCCAGCACCAGCTG GAGTCAGAGAAGTTCCCCCCCTTGTTCCCAGAGGGGCCAGCTCGGGCCTTTCATGAACTGTCCCGCGAG GAACAGGCGAAATACGAGAAGAGAAGGCTGGCGGATTACTGCCGGAAAGCCTACAAGAAGATCCACATC ACCAAGGTGGAAGAGCGTCTCACCACCATCTGCCAGCGGGAAAACTCCTTCTACGTGGACACCGTGCGT GCCTTCCGGGACAGGCGTTACGAGTTCAAAGGGCTCCACAAGGTGTGGAAAAAGAAGCTCTCGGCGGCC GTGGAGGTGGGCGACGCGGCTGAGGTGAAGCGCTGCAAGAACATGGAGGTGCTGTATGACTCGCTGCAG CTGGCCCACAAGTGCATCCTGAACTCCTTCTATGGCTATGTCATGCGCAAGGGGGCTCGCTGGTACTCC ATGGAGATGGCTGGCATCGTCTGCTTCACAGGGGCCAACATCATCACCCAGGCACGGGAGCTGATCGAG CAGATTGGGAGGCCCTTAGAGCTGGACACAGATGGTATATGGTGCGTCCTGCCCAACAGCTTCCCAGAA AATTTTGTCTTCAAGACGACCAATGTGAAGAAGCCCAAAGTGACCATCTCCTACCCAGGCGCCATGTTG AACATCATGGTCAAGGAAGGCTTCACCAATGACCAGTACCAGGAGCTGGCTGAGCCGTCCTCACTCACC TACGTCACCCGCTCAGAGAACAGCATCTTTTTTGAGGTTGATGGGCCCTACCTTGCCATGATTCTTCCA GCCTCCAAGGAAGAAGGCAAGAAATTGAAGAAGAGGTATGCTGTGTTCAATGAAGACGGTTCTCTGGCT GAGCTCAAGGGCTTTGAGGTCAAACGCCGCGGGGAACTGCAGCTGATTAAGATCTTCCAATCCTCGGTG TTTGAGGCCTTCCTCAAGGGCAGCACGCTGGAAGAGGTGTATGGCTCTGTAGCCAAGGTGGCTGACTAC TGGCTGGACGTGCTGTACAGCAAGGCAGCCAACATGCCTGACTCTGAGCTATTCGAGCTCATCTCTGAG AACCGTTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCAGAAGTCTACGTCCATCAGCACAGCAAAG CGCCTGGCCGAGTTCCTGGGAGACCAGATGGTCAAGGATGCAGGGCTGAGTTGCCGCTACATCATCTCC CGCAAGCCCGAGGGCTCCCCTGTCACGGAGAGGGCCATCCCACTTGCCATTTTCCAAGCAGAGCCCACG GTGAGGAAGCACTTTCTCCGGAAATGGCTCAAGAGCTCTTCCCTTCAAGACTTTGATATTCGAGCAATT CTGGATTGGGACTACTACATTGAGCGGCTGGGAAGCGCCATCCAGAAGATCATCACCATCCCTGCGGCC CTGCAGCAGGTAAAGAACCCAGTGCCACGTGTCAAACACCCCGACTGGCTGCACAAAAAACTGCTGGAG AAGAATGATGTCTACAAGCAGAAGAAGATCAGTGAGCTCTTCACCCTGGAGGGCAGGAGACAGGTCACG ATGGCCGAGGCCTCAGAAGACAGTCCGAGGCCAAGTGCTCCTGACATGGAGGACTTCGGCCTCGTAAAG CTGCCTCACCCAGCAGCCCCTGTCACTGTGAAGAGGAAGCGAGTTCTTTGGGAGAGCCAGGAGGAGTCC CAGGACCTCACGCCGACTGTGCCCTGGCAGGAAATCTTGGGGCAGCCTCCCGCCCTGGGAACCAGCCAG GAGGAATGGCTTGTCTGGCTCCGGTTCCACAAGAAGAAGTGGCAGCTGCAGGCCCGGCAGCGCCTCGCC CGCAGGAAGAGGCAGCGTCTGGAGTCGGCAGAGGGTGTGCTCAGGCCCGGGGCCATCCGGGATGGTCCT GCCACGGGGCTGGGGAGCTTCTTGCGAAGAACTGCCCGCAGCATCCTGGACCTTCCGTGGCAGATTGTG CAGATCAGCGAGACCAGCCAGGCCGGCCTGTTCAGGCTGTGGGCGCTCGTTGGCAGTGACTTGCACTGC ATCAGGCTGAGCATCCCCCGTGTGTTCTACGTGAACCAGCGAGTCGCTAAAGCGGAGGAGGGTGCTTCG TATCGCAAGGTAAATCGGGTCCTTCCTCGCTCCAACATGGTCTACAATCTCTATGAGTATTCAGTGCCA GAGGACATGTACCAGGAACACATCAACGAGATCAACGCTGAGCTGTCAGCGCCAGACATCGAGGGCGTA TATGAGACTCAGGTTCCGTTACTGTTCCGGGCCCTGGTGCACCTGGGCTGTGTGTGTGTGGTCAATAAA CAGCTGGTGAGGCACCTTTCAGGCTGGGAAGCAGAGACCTTTGCTCTTGAGCACCTGGAGATGCGCTCT CTGGCCCAGTTCAGCTACCTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCACCACGCACAGGCC CACAAAGCGCTCTTCGGGATCTTCATCCCCTCACAGCGCAGGGCATCCGTCTTTGTGCTGGACACTGTG CGCAGCAACCAGATGCCCAGCCTTGGCGCCCTGTACTCAGCAGAGCACGGCCTCCTCCTGGAGAAGGTG GGCCCTGAGCTCCTGCCACCCCCCAAACACACCTTCGAAGTTCGGGCAGAAACTGACCTGAAGACCATC TGCAGAGCCATCCAGCGATTCCTGCTCGCCTACAAGGAGGAGCGCCGGGGGCCCACACTCATCGCTGTT CAGTCCAGCTGGGAGCTGAAGAGGCTGGCCAGTGAAATTCCTGTCTTGGAGGAATTCCCACTGGTGCCT ATCTGTGTGGCTGACAAGATCAACTATGGGGTCCTGGACTGGCAGCGCCATGGAGCCCGGCGCATGATC CGTCACTACCTCAACCTGGACACCTGCCTGTCGCAGGCCTTCGAGATGAGCAGGTACTTTCACATTCCC ATTGGGAACCTACCAGAGGACATCTCCACATTCGGCTCCGACCTCTTCTTTGCCCGCCACCTCCAGCGC CACAACCACCTGCTCTGGCTGTCCCCTACAGCCCGCCCTGACCTGGGTGGAAAGGAGGCTGATGACAAC TGTCTTGTCATGGAGTTCGATGACCAAGCCACTGTTGAGATCAACAGTTCAGGCTGTTACTCCACAGTG TGTGTGGAGCTGGACCTTCAGAACCTGGCCGTCAACACCATTCTCCAGTCTCACCATGTCAACGACATG GAGGGGGCCGACAGCATGGGGATCAGCTTCGACGTGATCCAGCAGGCCTCCCTGGAGGACATGATCACG GGTGGTCAGGCTGCCAGTGCCCCGGCCAGCTACGATGAGACAGCCCTGTGCTCTAACACCTTCAGGATC CTGAAGAGCATGGTCGTGGGCTGGGTGAAGGAGATCACCCAGTACCACAACATCTATGCAGACAACCAG GTGATGCACTTCTACCGCTGGCTTCGGTCGCCATCCTCTCTGCTTCATGACCCTGCCCTGCACCGCACA CTCCACAACATGATGAAGAAGCTCTTCCTGCAGCTCATCGCTGAGTTCAAGCGCCTGGGGTCATCAGTC ATCTACGCCAACTTCAACCGCATCATCCTCTGTACAAAGAAGCGCCGTGTGGAAGATGCCATCGCTTAC GTGGAGTACATCACCAGCAGCATCCATTCAAAGGAGACCTTCCATTCTCTGACAATTTCTTTCTCTCGA TGCTGGGAATTTCTTCTCTGGATGGATCCATCTAACTATGGCGGAATCAAAGGAAAAGTTTCATCTCGT ATTCACTGTGGACTGCAAGACTCCCAGAAAGCAGGGGGAGCAGAGGATGAGCAGGAAAATGAGGACGAT GAGGAGGAAAGAGATGGGGAGGAGGAGGAAGAGGCGGAGGAATCCAACGTGGAGGATTTACTGGAAAAC AACTGGAACATTTTGCAGTTTTTGCCACAGGCAGCCTCCTGCCAGAACTACTTCCTCATGATTGTTTCA GCGTACATCGTGGCCGTGTACCACTGCATGAAGGACGGGCTGAGGCGCAGTGCTCCAGGGAGCACCCCC GTGAGGAGGAGGGGGGCCAGCCAGCTCTCCCAGGAGGCCGAGGGGGCGGTCGGAGCCCTTCCCGGAATG ATCACCTTCTCTCAGGATTATGTCGCAAATGAGCTCACTCAGAGCTTCTTCACCATCACTCAGAAGATT CAGAAGAAAGTCACAGGCTCTCGGAACTCCACTGAGCTCTCAGAGATGTTTCCTGTCCTCCCCGGTTCC CACTTGCTGCTCAATAACCCTGCCCTGGAGTTCATCAAATACGTGTGCAAGGTGCTGTCCCTGGACACC AACATCACAAACCAGGTGAATAAGCTGAACCGAGACCTGCTTCGCCTGGTGGATGTCGGCGAGTTCTCC GAGGAGGCCCAGTTCCGAGACCCCTGCCGCTCCTACGTGCTTCCTGAGGTCATCTGCCGCAGCTGTAAC TTCTGCCGCGACCTGGACCTGTGTAAAGACTCTTCCTTCTCAGAGGATGGGGCGGTCCTGCCTCAGTGG CTCTGCTCCAACTGTCAGGCGCCCTACGACTCCTCTGCCATCGAGATGACGCTGGTGGAAGTTCTACAG AAGAAGCTGATGGCCTTCACCCTGCAGGACCTGGTCTGCCTGAAGTGCCGCGGGGTGAAGGAGACCAGC ATGCCTGTGTACTGCAGCTGCGCGGGAGACTTCGCCCTCACCATCCACACCCAGGTCTTCATGGAACAG ATCGGAATATTCCGGAACATTGCCCAGCACTACGGCATGTCGTACCTCCTGGAGACCCTGGAGTGGCTG CTGCAGAAGAACCCACAGCTGGGCCATTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_006231 |
| Insert Size | 6861 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006231.3 |
| RefSeq Size | 8024 bp |
| RefSeq ORF | 6861 bp |
| Locus ID | 5426 |
| UniProt ID | Q07864 |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
| MW | 261.5 kDa |
| Gene Summary | This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224742 | POLE (Myc-DDK-tagged)-Human polymerase (DNA directed), epsilon (POLE) |
CNY 15,088.00 |
|
| RC224742L1 | Lenti ORF clone of Human polymerase (DNA directed), epsilon (POLE), Myc-DDK-tagged |
CNY 17,488.00 |
|
| RG224742 | POLE (tGFP-tagged) - Human polymerase (DNA directed), epsilon (POLE) |
CNY 16,688.00 |
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