CACNA1F (NM_005183) Human Untagged Clone

CAT#: SC303598

CACNA1F (untagged)-Human calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F)



  "NM_005183" in other vectors (3)

CNY 38,100.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AIED; Cav1.4; Cav1.4alpha1; COD3; COD4; CORDX; CORDX3; CSNB2; CSNB2A; CSNBX2; JM8; JMC8; OA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC303598 representing NM_005183.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCGGAATCTGAAGGCGGGAAAGACACCACCCCAGAGCCCAGTCCAGCCAATGGGGCAGGCCCTGGT
CCCGAATGGGGGCTGTGCCCCGGGCCCCCAGCTGTGGAAGGTGAAAGCAGTGGGGCATCAGGCCTAGGG
ACCCCTAAGCGAAGAAACCAGCACAGCAAGCACAAGACAGTGGCAGTGGCCAGTGCCCAGCGGTCACCT
CGGGCACTCTTCTGCCTCACCCTGGCCAATCCTCTGCGACGGTCCTGCATCAGCATCGTGGAGTGGAAG
CCCTTCGACATCCTCATCCTGCTGACCATCTTTGCCAACTGCGTGGCCCTGGGAGTTTACATCCCCTTC
CCTGAGGACGACTCCAACACTGCCAACCACAACCTGGAGCAGGTGGAGTACGTATTCCTGGTGATTTTC
ACTGTGGAGACGGTGCTCAAGATCGTGGCCTACGGGCTGGTGCTCCACCCCAGCGCCTACATCCGCAAT
GGCTGGAACCTACTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTTCTGCTGGAGCAGGGCCCC
GGACGGCCAGGCGACGCCCCGCACACCGGGGGAAAGCCAGGAGGCTTCGATGTGAAGGCATTGAGGGCG
TTTCGGGTGCTGCGGCCACTGAGGCTGGTGTCTGGGGTCCCGAGCCTGCACATAGTGCTCAATTCCATC
ATGAAGGCTCTGGTGCCGCTGCTGCACATTGCACTGCTCGTGCTCTTCGTCATCATCATTTATGCCATC
ATTGGGCTCGAGCTGTTCCTTGGACGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAAGCG
GAGGAGGACCCATCGCCCTGTGCGTCTTCGGGATCAGGGCGTGCGTGCACGCTGAACCAGACTGAGTGC
CGCGGGCGCTGGCCAGGGCCCAATGGAGGCATCACCAACTTTGACAACTTCTTCTTCGCCATGCTGACA
GTCTTCCAGTGTGTCACCATGGAAGGCTGGACCGATGTGCTCTACTGGATGCAAGATGCCATGGGGTAT
GAACTGCCCTGGGTGTACTTTGTGAGCCTTGTCATCTTTGGGTCCTTCTTCGTCCTCAACCTTGTGCTT
GGCGTCCTGAGTGGGGAGTTCTCCAAGGAGAGAGAGAAAGCGAAAGCTCGCGGGGACTTCCAGAAGCAG
CGGGAGAAGCAGCAGATGGAGGAAGACCTGCGGGGCTACCTGGACTGGATCACTCAAGCCGAAGAGCTG
GACATGGAGGACCCCTCCGCCGATGACAACCTTGGTTCTATGGCTGAAGAGGGCCGGGCGGGCCATCGG
CCACAGCTGGCCGAGCTGACCAATAGGAGGCGTGGACGTCTGCGCTGGTTCAGTCATTCTACTCGCTCC
ACACACTCCACCAGCAGCCATGCCAGCCTCCCAGCCAGTGACACCGGTTCCATGACAGAGACCCAAGGC
GATGAGGATGAGGAGGAGGGGGCTCTGGCCAGCTGTACACGCTGCCTAAACAAGATCATGAAAACCAGA
GTCTGCCGCCGCCTCCGCCGAGCCAACCGGGTCCTTCGGGCACGCTGCCGTCGGGCAGTGAAGTCCAAT
GCCTGCTACTGGGCTGTGCTGTTGCTCGTCTTCCTCAACACGTTGACCATCGCCTCTGAGCACCACGGG
CAGCCTGTGTGGCTCACCCAGATCCAGGAGTATGCCAACAAAGTGTTGCTCTGTCTGTTCACGGTGGAG
ATGCTTCTCAAATTGTACGGTCTGGGCCCCTCTGCCTATGTGTCTTCCTTCTTCAACCGCTTTGACTGC
TTTGTGGTCTGTGGGGGCATCCTAGAGACCACCTTGGTGGAGGTGGGTGCCATGCAGCCCTTGGGCATC
TCAGTGCTCCGATGTGTGCGCCTCCTCAGGATCTTTAAGGTCACCAGACACTGGGCTTCTCTGAGCAAT
CTGGTGGCATCCCTGCTCAATTCAATGAAATCCATCGCATCCTTGCTGCTTCTCCTCTTCCTCTTCATC
ATTATCTTCTCCCTGCTTGGCATGCAGCTGTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACCAAG
CGAAGCACCTTTGACACGTTCCCCCAGGCCCTCCTCACTGTCTTTCAGATCCTGACAGGTGAGGACTGG
AACGTGGTCATGTATGATGGTATCATGGCATATGGTGGCCCCTTCTTCCCAGGAATGTTGGTGTGCATC
TATTTCATCATTCTCTTCATCTGTGGCAACTACATCCTGTTGAACGTGTTTCTTGCCATTGCTGTGGAC
AACCTGGCCAGTGGAGATGCAGGCACTGCCAAGGACAAGGGCGGGGAGAAGAGCAATGAGAAGGATCTC
CCACAGGAGAATGAAGGCCTGGTGCCTGGTGTGGAGAAAGAGGAAGAGGAGGGTGCAAGGAGGGAAGGA
GCAGACATGGAGGAGGAGGAGGAGGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAGGGTGCAGGG
GGTGTGGAACTCCTGCAGGAAGTTGTACCCAAGGAGAAGGTGGTACCCATCCCTGAGGGCAGCGCCTTC
TTCTGCCTCAGCCAAACCAACCCGCTGAGGAAGGGCTGCCACACCCTCATCCACCATCATGTCTTCACC
AATCTTATCCTGGTGTTCATCATCCTCAGCAGTGTGTCCCTGGCCGCTGAGGACCCCATCCGAGCCCAC
TCCTTCCGCAACCATATTCTGGGTTACTTCGATTATGCCTTCACCTCCATTTTCACTGTGGAGATTCTA
CTAAAGATGACAGTGTTTGGGGCCTTCCTGCACCGCGGCTCCTTCTGCCGTAGCTGGTTTAATATGTTG
GATCTGCTGGTGGTCAGTGTGTCCCTCATCTCCTTTGGCATCCACTCCAGCGCCATCTCGGTGGTGAAG
ATTCTGCGAGTACTCCGAGTACTGCGGCCCCTCCGAGCCATCAACAGGGCCAAGGGACTCAAGCATGTG
GTGCAGTGTGTATTTGTGGCCATCCGGACCATCGGAAACATCATGATTGTCACCACACTTCTGCAATTT
ATGTTCGCCTGCATCGGGGTGCAGCTCTTCAAGGGGAAATTCTACACCTGCACGGACGAGGCCAAACAC
ACCCCTCAAGAATGCAAGGGCTCCTTCCTGGTATACCCAGATGGAGACGTGTCACGGCCCCTGGTCCGG
GAGCGGCTCTGGGTCAACAGTGATTTCAACTTTGACAATGTCCTTTCAGCCATGATGGCCCTGTTCACT
GTCTCCACCTTTGAAGGCTGGCCTGCACTGCTATACAAGGCCATCGATGCATATGCAGAGGACCACGGC
CCCATCTATAATTACCGTGTGGAGATCTCAGTGTTCTTCATTGTCTACATCATCATCATTGCGTTCTTC
ATGATGAACATCTTCGTGGGCTTCGTCATCATCACTTTCCGTGCCCAGGGCGAGCAGGAGTACCAAAAC
TGTGAGCTGGACAAGAACCAGCGTCAATGTGTGGAATATGCCCTCAAGGCCCAGCCACTCCGCCGTTAC
ATCCCCAAGAACCCGCATCAGTATCGTGTGTGGGCCACTGTGAACTCTGCTGCCTTTGAGTACCTGATG
TTCCTGCTCATCCTGCTCAACACAGTTGCCCTAGCCATGCAGCACTATGAGCAGACTGCTCCCTTCAAC
TATGCCATGGACATCCTCAACATGGTCTTCACTGGCCTCTTCACTATTGAGATGGTGCTCAAAATCATC
GCCTTCAAGCCCAAGCATTACTTCACTGATGCCTGGAACACGTTTGACGCTCTTATTGTGGTGGGCAGC
ATAGTGGATATTGCCGTCACTGAAGTCAATAATGGTGGCCACCTTGGCGAGAGCTCTGAGGACAGCTCC
CGCATTTCCATTACCTTCTTTCGCCTCTTCCGAGTTATGCGGCTGGTCAAGCTTCTCAGTAAGGGTGAA
GGGATCCGCACATTGCTCTGGACATTCATCAAGTCCTTCCAGGCCTTGCCCTATGTGGCTCTTCTCATC
GCAATGATATTCTTCATCTATGCCGTCATTGGCATGCAGATGTTCGGCAAGGTGGCTCTTCAGGATGGC
ACACAGATAAACCGAAACAACAACTTCCAGACCTTTCCACAGGCTGTGCTGCTTCTGTTCAGGTGTGCC
ACTGGTGAGGCATGGCAGGAGATAATGCTTGCCAGCCTTCCCGGAAATCGGTGTGATCCTGAGTCTGAC
TTCGGCCCTGGTGAAGAGTTTACCTGTGGTAGCAATTTTGCCATCGCCTATTTCATCAGCTTCTTCATG
CTCTGTGCCTTCCTGATCATAAATCTCTTTGTGGCTGTGATCATGGACAACTTTGATTATCTCACCAGA
GATTGGTCCATCCTGGGCCCCCATCACCTTGATGAATTCAAGAGGATCTGGTCTGAATATGACCCTGGG
GCCAAGGGCCGCATCAAACACTTGGATGTGGTTGCCCTGCTGAGACGTATCCAGCCCCCTCTGGGATTT
GGGAAGCTGTGCCCACACCGAGTGGCCTGCAAGAGACTTGTGGCAATGAACATGCCCCTCAACTCAGAT
GGGACGGTGACATTCAACGCCACACTCTTTGCCCTGGTCCGGACATCCCTGAAGATCAAAACAGAAGGG
AACCTGGAGCAAGCCAACCAGGAGCTGCGGATTGTCATCAAAAAGATCTGGAAGCGGATGAAACAGAAG
CTGCTAGATGAGGTCATCCCCCCACCAGACGAGGAGGAGGTCACCGTGGGCAAATTCTACGCCACATTT
CTGATCCAGGACTATTTCCGCAAATTCCGGCGGAGGAAAGAAAAAGGGCTACTAGGCAACGACGCCGCC
CCTAGCACCTCTTCCGCCCTTCAGGCTGGTCTGCGGAGCCTGCAGGACTTGGGTCCTGAGATGCGGCAG
GCCCTCACCTGTGACACAGAGGAGGAGGAAGAAGAGGGGCAGGAGGGAGTGGAGGAGGAAGATGAAAAG
GACTTGGAAACTAACAAAGCCACGATGGTCTCCCAGCCCTCAGCTCGCCGGGGCTCCGGGATTTCTGTG
TCTCTGCCTGTCGGGGACAGACTTCCAGATTCACTCTCCTTTGGGCCCAGTGATGATGACAGGGGGACT
CCCACCTCCAGTCAGCCCAGTGTGCCCCAGGCTGGATCCAACACCCACAGGAGAGGCTCTGGGGCTCTC
ATTTTCACCATCCCAGAAGAAGGAAATTCTCAGCCCAAGGGAACCAAAGGGCAAAACAAGCAAGATGAG
GATGAGGAAGTCCCTGATCGGCTTTCCTACCTAGATGAGCAGGCAGGGACTCCCCCGTGCTCAGTCCTT
TTGCCACCTCACAGAGCTCAGAGATACATGGATGGGCACCTGGTACCACGCCGCCGTCTGCTGCCCCCC
ACACCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAGCGCCAGGGCAGTTGTGAGGATTTA
CCCATCCCAGGCACCTATCATCGTGGGCGAAATTCAGGGCCCAATAGGGCTCAGGGTTCCTGGGCAACA
CCACCTCAGCGGGGTCGGCTCCTGTATGCCCCGCTGTTGTTGGTGGAAGAGGGCGCAGCGGGGGAGGGG
TACCTCGGCAGATCCAGTGGCCCACTGCGCACCTTCACCTGTCTGCACGTGCCTGGAACCCACTCGGAC
CCCAGCCATGGGAAGAGGGGCAGTGCCGACAGCTTGGTGGAGGCTGTGCTTATCTCAGAGGGTCTGGGC
CTCTTTGCTCGAGACCCACGTTTCGTGGCCCTGGCCAAGCAGGAGATTGCAGATGCGTGTCGCCTGACG
CTGGATGAGATGGACAATGCTGCCAGTGACCTGCTGGCACAGGGAACCAGCTCTCTCTATAGCGACGAG
GAGTCCATCCTCTCCCGCTTCGATGAGGAGGACTTGGGAGACGAGATGGCCTGCGTCCACGCCCTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_005183
Insert Size 5934 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005183.3
RefSeq Size 6070 bp
RefSeq ORF 5934 bp
Locus ID 778
UniProt ID O60840
Protein Families Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction
MW 220.7 kDa
Gene Summary This gene encodes a multipass transmembrane protein that functions as an alpha-1 subunit of the voltage-dependent calcium channel, which mediates the influx of calcium ions into the cell. The encoded protein forms a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Mutations in this gene can cause X-linked eye disorders, including congenital stationary night blindness type 2A, cone-rod dystropy, and Aland Island eye disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2013]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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