DOCK3 (NM_004947) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MOCA; NEDIDHA; PBP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC303553 representing NM_004947. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGACCCCCACGGAGGAGGAGAAATACGGCGTAGTGATATGCAGCTTTCGAGGATCTGTCCCTCAA GGGTTGGTCTTAGAAATAGGAGAAACAGTCCAGATTCTTGAAAAATGTGAAGGTTGGTACAGAGGAGTT TCAACAAAGAAGCCAAATGTGAAGGGGATCTTTCCTGCAAATTACATTCACTTGAAAAAGGCAATTGTC AGTAATAGGGGGCAGTATGAAACTGTGGTTCCACTTGAAGATTCTATTGTGACTGAGGTTACAGCAACT CTACAAGAATGGGCAAGTTTGTGGAAACAGTTGTATGTGAAACACAAAGTAGATCTTTTCTACAAACTA CGCCATGTGATGAATGAACTTATTGACCTGCGAAGGCAGCTACTGTCTGGTCACCTGACTCAGGATCAG GTGCGGGAGGTTAAGCGGCACATCACCGTGCGCCTGGACTGGGGTAATGAACATTTGGGCCTGGACCTG GTGCCTCGGAAGGACTTTGAAGTAGTGGACTCGGACCAGATTAGTGTCTCAGATCTCTATAAGATGCAT TTATCTAGCCGGCAGAGTGTACAGCAAAGCACATCCCAGGTAGATACAATGCGCCCACGTCATGGGGAA ACATGTCGGATGCCAGTGCCACATCACTTCTTCCTCAGCCTGAAGAGTTTCACTTACAATACTATTGGG GAAGATACCGATGTCTTCTTTTCCTTATATGACATGAGGGAAGGCAAGCAGATCAGTGAGCGGTTTCTG GTAAGACTGAACAAGAATGGTGGGCCGAGGAACCCAGAGAAGATAGAACGAATGTGTGCCCTTTTTACA GATCTGAGCAGCAAAGATATGAAGAGAGATTTGTATATCGTTGCCCATGTGATCCGAATAGGCCGGATG CTCCTGAACGACTCAAAGAAAGGTCCTCCTCACCTGCACTACAGGCGACCATATGGCTGTGCGGTCCTA AGCATCTTGGATGTCCTACAGTCACTCACAGAAGTAAAGGAAGAAAAGGATTTTGTTCTTAAGGTTTAC ACGTGCAACAACGAGAGTGAGTGGTCCCAGATCCACGAGAACATCATCCGAAAGTCCAGTGCCAAGTAC TCTGCCCCCAGCGCCAGCCATGGTCTTATCATTTCTCTGCAGCTTCTTCGTGGAGACATGGAACAGATT CGGAGAGAAAATCCCATGATATTTAATAGGGGATTGGCAATTACAAGAAAATTGGGATTTCCTGATGTC ATTATGCCAGGTGATATCCGCAATGACCTGTACCTAACCCTGGAGAAGGGGGATTTCGAGAGAGGAGGA AAGAGTGTACAAAAGAATATTGAAGTGACCATGTATGTGCTTTATGCAGATGGAGAAATCTTGAAGGAT TGCATCAGCTTGGGTTCAGGAGAGCCAAATAGGAGTTCCTACCACTCCTTTGTCCTCTACCACAGTAAT AGTCCTCGCTGGGGAGAAATTATCAAATTGCCTATCCCCATTGACCGGTTCCGGGGCTCCCACCTGCGC TTTGAGTTCAGACATTGTTCCACAAAGGACAAAGGGGAAAAGAAACTCTTTGGCTTTGCATTCTCAACC CTGATGCGTGATGATGGCACCACCCTCTCAGATGATATTCACGAGCTTTATGTGTACAAGTGTGATGAG AATAGCACGTTTAATAACCATGCTCTGTACCTGGGCCTGCCCTGCTGCAAAGAGGACTACAATGGCTGC CCTAATATTCCTTCTAGCCTCATCTTCCAGCGCAGCACCAAAGAGTCTTTCTTCATCTCCACTCAGCTC TCCTCTACCAAACTCACCCAGAATGTGGACCTCCTAGCTCTGCTGAAGTGGAAAGCCTTCCCCGACCGG ATCATGGATGTACTAGGGCGGCTGCGGCATGTCAGTGGGGAGGAAATTGTTAAGTTTCTGCAGGACATC TTAGATACACTCTTTGTGATTTTGGATGATAATACAGAGAAGTACGGCCTGTTGGTTTTTCAGTCTCTG GTGTTCATCATCAACCTGCTCCGAGACATCAAGTATTTTCACTTTCGACCTGTGATGGACACGTATATC CAGAAGCACTTTGCTGGAGCTCTGGCATACAAGGAGCTCATCCGCTGTTTGAAGTGGTATATGGACTGC TCAGCAGAACTGATTCGACAGGACCACATTCAAGAAGCTATGCGGGCCTTGGAGTACCTTTTCAAGTTC ATTGTACAGTCACGGATCCTGTACTCACGAGCCACTTGTGGAATGGAAGAGGAACAATTCAGATCCAGT ATCCAAGAACTTTTCCAGTCCATCCGGTTTGTGCTCAGTCTGGACAGCCGAAACTCAGAAACACTCCTT TTTACTCAGGCTGCACTCCTCAATTCTTTCCCAACCATCTTTGATGAGCTTCTGCAAATGTTCACCGTG CAAGAGGTGGCAGAGTTTGTGAGAGGGACACTGGGGAGCATGCCCAGCACTGTGCACATTGGGCAGTCA ATGGACGTGGTCAAGCTGCAGTCCATTGCCAGGACAGTGGATAGCCGCCTGTTTTCTTTCTCAGAATCC CGCCGCATCCTGCTTCCTGTGGTTCTCCATCACATTCACCTTCACCTGAGGCAGCAGAAAGAGCTGCTA ATTTGCTCAGGGATTCTTGGCAGCATCTTCTCCATCGTCAAGACCAGCTCTCTGGAGGCAGATGTCATG GAGGAAGTAGAGATGATGGTGGAGAGCCTCCTGGACGTGCTCTTGCAGACTCTGCTCACCATCATGAGC AAATCGCACGCTCAGGAGGCGGTAAGAGGGCAGCGGTGCCCGCAGTGCACAGCCGAGATCACTGGCGAG TATGTGTCCTGCCTTCTCTCACTGCTCCGCCAGATGTGTGACACCCATTTCCAGCACCTCCTGGACAAC TTCCAGAGCAAAGATGAACTCAAGGAATTTCTGCTGAAGATTTTTTGCGTGTTCCGGAACCTGATGAAG ATGAGTGTCTTCCCTCGGGACTGGATGGTAATGAGACTGCTCACAAGCAATATTATAGTCACTACTGTC CAGTACCTGTCCTCTGCACTGCACAAGAATTTCACAGAGACTGACTTTGACTTTAAGGTGTGGAATTCT TACTTTAGCCTGGCAGTTCTATTCATAAATCAGCCAAGCCTTCAGCTAGAAATTATCACCTCAGCCAAA AGGAAGAAGATTCTAGATAAGTATGGGGACATGCGTGTAATGATGGCCTATGAACTGTTCAGCATGTGG CAGAATTTGGGTGAACATAAGATCCACTTTATTCCGGGAATGATTGGTCCTTTTCTGGGTGTGACACTG GTCCCACAGCCAGAAGTACGGAATATCATGATTCCCATCTTTCATGACATGATGGACTGGGAGCAGAGA AAAAATGGCAACTTCAAACAGGTGGAGGCCGAGTTGATTGACAAGCTGGACAGCATGGTGTCAGAAGGG AAAGGTGACGAGAGCTACAGGGAGCTCTTCAGCCTACTAACCCAGCTGTTTGGGCCCTACCCCAGCCTG CTGGAGAAGGTTGAACAAGAAACATGGCGCGAGACCGGCATTTCCTTTGTGACCTCAGTCACCCGCCTC ATGGAACGTCTTCTTGACTACAGGGACTGCATGAAAGGAGAGGAAACAGAGAATAAGAAGATAGGCTGC ACTGTTAACCTGATGAATTTTTACAAATCTGAGATTAACAAGGAAGAAATGTATATCCGCTACATCCAT AAGCTTTGTGACATGCACTTGCAGGCCGAAAACTACACAGAGGCCGCATTTACCCTGCTCCTTTACTGT GAGCTGCTGCAGTGGGAGGACCGGCCACTACGGGAATTCCTCCACTACCCATCGCAGACAGAGTGGCAG CGGAAGGAGGGACTGTGCCGGAAGATCATTCACTACTTCAACAAAGGCAAGAGCTGGGAGTTTGGGATC CCACTGTGCAGGGAGCTGGCGTGTCAGTACGAGAGCCTCTATGATTACCAGAGCCTCAGCTGGATTCGG AAAATGGAGGCCAGCTACTATGACAACATTATGGAGCAGCAACGCCTGGAGCCTGAGTTCTTTCGGGTC GGCTTCTATGGCAGGAAGTTTCCTTTCTTTCTTCGGAACAAAGAATACGTGTGCCGTGGCCATGACTAC GAGAGGCTGGAGGCCTTCCAGCAGAGGATGCTCAGTGAGTTTCCGCAGGCTGTCGCCATGCAGCACCCC AACCATCCTGATGACGCCATCCTACAGTGCGATGCCCAGTACTTGCAGATCTATGCAGTGACGCCCATT CCAGATTATGTGGATGTTCTGCAGATGGATAGGGTACCAGATCGAGTCAAGAGCTTCTATCGCGTCAAC AATGTGAGGAAGTTCCGGTATGACAGGCCTTTTCACAAAGGCCCCAAGGACAAGGAGAATGAATTCAAG AGCCTGTGGATTGAACGTACCACACTGACCCTGACCCACAGCTTGCCTGGCATCTCTCGGTGGTTTGAA GTGGAGAGGAGGGAACTGGTGGAGGTGAGCCCTCTGGAGAATGCCATCCAAGTGGTTGAGAATAAGAAC CAGGAGCTACGCTCCCTGATCAGCCAGTATCAACACAAGCAGGTGCATGGCAACATTAACCTGCTAAGC ATGTGCCTGAATGGTGTCATTGATGCAGCTGTCAATGGAGGCATTGCACGCTATCAGGAGGCCTTCTTT GATAAAGATTACATCAACAAGCACCCAGGAGATGCTGAGAAGATCACCCAGCTCAAGGAGCTTATGCAG GAGCAGGTTCATGTCCTTGGAGTTGGGCTAGCAGTTCATGAGAAGTTTGTGCACCCAGAAATGCGGCCT CTGCATAAGAAGCTAATTGATCAGTTCCAGATGATGCGGGCCAGTCTCTACCATGAGTTTCCAGGTTTG GATAAGCTAAGTCCTGCATGTTCAGGCACCAGCACCCCACGGGGAAATGTTCTGGCATCCCATAGCCCC ATGAGTCCGGAGAGCATCAAGATGACCCACCGGCACAGCCCCATGAACTTGATGGGCACAGGCCGCCAT TCATCATCCTCTCTCTCCTCACATGCGTCTAGTGAAGCAGGAAACATGGTGATGCTGGGTGACGGCTCC ATGGGTGATGCTCCTGAGGACCTGTACCACCACATGCAGCTCGCGTATCCCAACCCCAGGTACCAAGGC TCAGTCACCAACGTCTCTGTTCTGTCCTCGTCCCAGGCAAGCCCTTCTTCCTCCAGCCTGAGTTCCACT CACTCAGCACCATCCCAGATGATTACCTCTGCCCCTTCCAGTGCCCGAGGCTCTCCCTCTCTGCCAGAT AAGTACCGCCATGCCCGTGAAATGATGTTGTTGCTGCCCACATACCGGGACCGCCCAAGCAGTGCCATG TATCCAGCAGCCATCCTGGAGAACGGACAGCCGCCGAATTTCCAGCGAGCCCTGTTCCAGCAAGTGGTC GGAGCCTGCAAACCCTGCAGTGATCCCAATCTGTCTGTGGCTGAAAAAGGTCATTACTCCCTACACTTT GACGCCTTCCACCACCCTCTGGGTGATACCCCCCCAGCCCTCCCTGCCCGGACCCTGCGCAAGTCTCCT CTCCACCCTATCCCAGCCTCCCCCACAAGCCCCCAGTCAGGTCTGGACGGCAGCAACTCTACGCTGTCC GGCAGTGCCAGCAGCGGCGTGTCCTCCTTGAGTGAGAGTAACTTTGGGCACTCCTCGGAGGCCCCACCT CGCACTGACACCATGGACTCCATGCCAAGTCAGGCCTGGAATGCTGACGAAGATCTTGAGCCACCCTAC CTCCCTGTCCACTACAGCCTCTCTGAGTCTGCCGTCCTGGACTCCATCAAGGCCCAGCCATGCCGAAGC CACTCAGCCCCAGGGTGCGTCATCCCTCAGGACCCCATGGACCCGCCTGCGCTGCCGCCCAAGCCCTAC CACCCCCGCCTGCCGGCCCTGGAGCACGATGAGGGGGTGCTGCTGCGTGAAGAGACTGAGAGGCCTCGA GGCCTGCACCGCAAGGCTCCATTGCCTCCTGGGAGCGCTAAGGAGGAGCAGGCCCGCATGGCCTGGGAG CACGGCCGAGGGGAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_004947
Insert Size	6093 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_004947.4
RefSeq Size	8777 bp
RefSeq ORF	6093 bp
Locus ID	1795
UniProt ID	Q8IZD9
MW	233.1 kDa
Gene Summary	This gene is specifically expressed in the central nervous system (CNS). It encodes a member of the DOCK (dedicator of cytokinesis) family of guanine nucleotide exchange factors (GEFs). This protein, dedicator of cytokinesis 3 (DOCK3), is also known as modifier of cell adhesion (MOCA) and presenilin-binding protein (PBP). The DOCK3 and DOCK1, -2 and -4 share several conserved amino acids in their DHR-2 (DOCK homology region 2) domains that are required for GEF activity, and bind directly to WAVE proteins [Wiskott-Aldrich syndrome protein (WASP) family Verprolin-homologous proteins] via their DHR-1 domains. The DOCK3 induces axonal outgrowth in CNS by stimulating membrane recruitment of the WAVE complex and activating the small G protein Rac1. This gene is associated with an attention deficit hyperactivity disorder-like phenotype by a complex chromosomal rearrangement. [provided by RefSeq, Aug 2010]

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